This study compared the clinical outcomes of the frozen-thawed cycles of high-quality cleavage embryos with low-quality blastocysts to provide a reference for the choice of frozen-thawed embryo transfer schemes and to...This study compared the clinical outcomes of the frozen-thawed cycles of high-quality cleavage embryos with low-quality blastocysts to provide a reference for the choice of frozen-thawed embryo transfer schemes and to improve clinical pregnancy rates.A retrospective analysis was performed on the clinical data of patients undergoing frozen-thawed embryo transfer at the Reproductive Medicine Center of Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology from 2016 to 2017.In total,845 cases were divided into a high-quality cleavage embryo group(group A)and a low-quality blastocyst group(group B).Each group was further divided into subgroups based on the number of transplants.Group A was categorized into two subgroups comprising of 94 cases in subgroup Al(1 high-quality 8-cell group)and 201 cases in subgroup A2(2 high-quality 8-cell group).Group B was divided into four subgroups consisting of 73 cases in subgroup B I(D53BC group),65 cases in subgroup B2(D54BC group),110 cases in subgroup B3(D63BC group),and 282 cases in subgroup B4(D64BC group).The pregnancy outcomes and neonatal outcomes between the groups were compared.The clinical pregnancy rates(56.72%and 60.00%)and live birth rates(47.76%and 46.15%)in subgroups A2 and B2 showed no significant differences,but these rates were significantly higher in subgroups A2 and B2 than in the rest subgroups(P<0.05).The multiple birth rate(26.32%)in the subgroup A2 was significantly higher than that in the rest subgroups(P<0.05).There were no statistically significant differences in the abortion rates among all groups(P>0.05).In terms of neonatal outcomes,there were no statistically significant differences in the proportion of premature births,sex ratios,and birth defects among the low-weight and gigantic infants(P>0.05).Transplanting two high-quality cleavage embryos during the frozen-thawed embryo transfer cycles could significantly increase clinical pregnancy rates and live birth rates,but at the same time,it also increased the risks of multiple births and complications to mothers and infants.The D54BC subgroup had the most significant advantages among all groups(P<0.05).The rest low-quality blastocysts had clinical outcomes similar to the single high-quality cleavage embryo group.展开更多
目的比较玻璃化冻融D 3卵裂期胚胎当日解冻和提前解冻移植的结局。方法回顾性分析2014年1月至2016年3月在沈阳东方菁华医院进行的398例冻胚复苏移植周期的临床资料,根据解冻时间不同分为两组:过夜培养组(提前解冻过夜培养后移植,346例)...目的比较玻璃化冻融D 3卵裂期胚胎当日解冻和提前解冻移植的结局。方法回顾性分析2014年1月至2016年3月在沈阳东方菁华医院进行的398例冻胚复苏移植周期的临床资料,根据解冻时间不同分为两组:过夜培养组(提前解冻过夜培养后移植,346例);当日解冻组(当日解冻移植,52例)。又将上述两组根据解冻前胚胎评分情况分为3组:优胚组共293例(≥1个6细胞Ⅱ级胚胎);混合胚组共86例(1个≥6细胞Ⅱ级胚胎+≥1个一般质量胚胎)和一般质量胚胎组共19例。比较各组间的移植结局。结果优胚组和一般质量胚胎组中,过夜培养组种植率和临床妊娠率略高于当日解冻组,而混合胚组中当日解冻组的种植率和临床妊娠率略高于过夜培养组,差异均无统计学意义(P>0.05)。结论 D 3卵裂期胚胎提前解冻过夜培养并不能显著提高种植率和临床妊娠率,故在平常工作中,可根据患者自身情况以及实验室的工作安排,选择合适的解冻时间。展开更多
基金This project was supported by grants from National Key R&D Program of China(No.2018YFC1002103)Natural Science Foundation of China(No.81801531).
文摘This study compared the clinical outcomes of the frozen-thawed cycles of high-quality cleavage embryos with low-quality blastocysts to provide a reference for the choice of frozen-thawed embryo transfer schemes and to improve clinical pregnancy rates.A retrospective analysis was performed on the clinical data of patients undergoing frozen-thawed embryo transfer at the Reproductive Medicine Center of Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology from 2016 to 2017.In total,845 cases were divided into a high-quality cleavage embryo group(group A)and a low-quality blastocyst group(group B).Each group was further divided into subgroups based on the number of transplants.Group A was categorized into two subgroups comprising of 94 cases in subgroup Al(1 high-quality 8-cell group)and 201 cases in subgroup A2(2 high-quality 8-cell group).Group B was divided into four subgroups consisting of 73 cases in subgroup B I(D53BC group),65 cases in subgroup B2(D54BC group),110 cases in subgroup B3(D63BC group),and 282 cases in subgroup B4(D64BC group).The pregnancy outcomes and neonatal outcomes between the groups were compared.The clinical pregnancy rates(56.72%and 60.00%)and live birth rates(47.76%and 46.15%)in subgroups A2 and B2 showed no significant differences,but these rates were significantly higher in subgroups A2 and B2 than in the rest subgroups(P<0.05).The multiple birth rate(26.32%)in the subgroup A2 was significantly higher than that in the rest subgroups(P<0.05).There were no statistically significant differences in the abortion rates among all groups(P>0.05).In terms of neonatal outcomes,there were no statistically significant differences in the proportion of premature births,sex ratios,and birth defects among the low-weight and gigantic infants(P>0.05).Transplanting two high-quality cleavage embryos during the frozen-thawed embryo transfer cycles could significantly increase clinical pregnancy rates and live birth rates,but at the same time,it also increased the risks of multiple births and complications to mothers and infants.The D54BC subgroup had the most significant advantages among all groups(P<0.05).The rest low-quality blastocysts had clinical outcomes similar to the single high-quality cleavage embryo group.
文摘目的比较玻璃化冻融D 3卵裂期胚胎当日解冻和提前解冻移植的结局。方法回顾性分析2014年1月至2016年3月在沈阳东方菁华医院进行的398例冻胚复苏移植周期的临床资料,根据解冻时间不同分为两组:过夜培养组(提前解冻过夜培养后移植,346例);当日解冻组(当日解冻移植,52例)。又将上述两组根据解冻前胚胎评分情况分为3组:优胚组共293例(≥1个6细胞Ⅱ级胚胎);混合胚组共86例(1个≥6细胞Ⅱ级胚胎+≥1个一般质量胚胎)和一般质量胚胎组共19例。比较各组间的移植结局。结果优胚组和一般质量胚胎组中,过夜培养组种植率和临床妊娠率略高于当日解冻组,而混合胚组中当日解冻组的种植率和临床妊娠率略高于过夜培养组,差异均无统计学意义(P>0.05)。结论 D 3卵裂期胚胎提前解冻过夜培养并不能显著提高种植率和临床妊娠率,故在平常工作中,可根据患者自身情况以及实验室的工作安排,选择合适的解冻时间。
