Acrylamide(AA)is ubiquitous in packaging materials,paints,cosmetics,food and drinking water.However,AA has obvious neurotoxicity,reproductive toxicity and carcinogenicity,which seriously endangers human health.As one ...Acrylamide(AA)is ubiquitous in packaging materials,paints,cosmetics,food and drinking water.However,AA has obvious neurotoxicity,reproductive toxicity and carcinogenicity,which seriously endangers human health.As one of precious traditional Chinese medicines,Gastrodia elata Bl.has been mainly used to lower blood pressure and resist myocardial ischemia.However,the antioxidant activity of Gastrodia elata Bl.on AA-induced DNA damage has not been reported.We found that AA(25,30 and 50 mg/kg)could cause DNA damage and the damage was time/dose dependent.After AA administration,Tail moment,micronuclei formation rate,8-OHdG and MDA level were significantly increased and SOD activity was decreased.Gastrodia elata Bl.decoction,gastrodin and vitamin C could reduce AA-induced DNA damage by decreasing AA-induced increment of tail moment and micronuclei formation rate.What’s more,Gastrodia elata Bl.decoction,gastrodin and vitamin C could lower 8-OHdG and MDA level and improve SOD activity.In conclusion,our research showed that Gastrodia elata Bl.decoction and gastrodin had protective effects on AA-induced DNA damage,and the potential protective mechanism might be related to anti-oxidative stress.展开更多
A genomic DNA containing 5'-upstream region and complete open reading frame of a Gastrodia antifungal protein was isolated by screening of a genomic library from Gastrodia elata B1. To investigate the promoter act...A genomic DNA containing 5'-upstream region and complete open reading frame of a Gastrodia antifungal protein was isolated by screening of a genomic library from Gastrodia elata B1. To investigate the promoter activity, the 5'-flanking region - 1 157 lip upstream from the putative transcription start site was fused to the coding sequence of beta-glucuronidase (GUS) gene and transformed into Nicotiana tabacum. The strongest GUS activity was detected in the roots of transgenic tobacco, followed by stems. The leaves only showed a low GUS activity. Furthermore, the promoter established inducible expression pattern in transgenic tobacco upon fungus Trichoderma viride inoculation and jasmonic acid and salicylic acid treatments.展开更多
为探究天麻(Gastrodia elata Bl.)质量评价的重要指标,本研究测定了27份陕西省汉中市天麻样品的外观性状、气味、颜色、含水量、浸出物和主要有效成分的含量,采用主成分分析(Principal Component Analysis,PCA)法确定其主要成分,结合熵...为探究天麻(Gastrodia elata Bl.)质量评价的重要指标,本研究测定了27份陕西省汉中市天麻样品的外观性状、气味、颜色、含水量、浸出物和主要有效成分的含量,采用主成分分析(Principal Component Analysis,PCA)法确定其主要成分,结合熵权TOPSIS(Technique for Order Preference by Similarity to an Ideal Solution)法得出天麻质量评价体系中各评价指标的权重,并对天麻质量进行综合评价。结果表明汉中市宁强县、略阳县、勉县产出的天麻在块茎粗、螺环纹数、鲜重、干重、颜色明亮度和总色度值上有一定差异;水分、浸出物含量以略阳县最高;天麻素与对羟基苯甲醇总含量以宁强县最高,勉县所产天麻的天麻素与对羟基苯甲醇含量接近,其成分含量比例与其他县差异显著;此外,宁强县所产天麻的巴利森苷类成分含量最高。PCA熵权TOPSIS法评价结果显示,宁强县、略阳县天麻排名靠前,各项指标中颜色因子、巴利森苷类因子、气味因子权重较大,因此天麻质量评价应注重颜色、气味以及巴利森苷类成分含量。展开更多
Gastrodia elata Bl.is a traditional Chinese herbal medicine with special pharmacological effects on headache and numbness.However,its wild sources are now in short supply because of over-excavation.A total of 14 endop...Gastrodia elata Bl.is a traditional Chinese herbal medicine with special pharmacological effects on headache and numbness.However,its wild sources are now in short supply because of over-excavation.A total of 14 endophytic fungi were isolated from Gastrodia elata Bl collected from Yinjing,in which 10 strains were identified,including Neonectria,Ascomycota,Fusarium,Oidiodendron and Dothiorella.All crude extracts showed some degrees of antioxidant activities by T-AOC method and DPPH methods,especially those from Oidiodendron GeR6 and Ascomycota sp.GeR2,which might be attributed due to their high phenolic contents.However,only Oidiodendron GeR6 contained gastrodin(39.2μg/g)as the host plant in the crude extracts.In conclusion,the notable activities of GeR6 indicated its medical use as a natural antioxidant agent,as well as substitutable use for Gastrodia elata Bl.in the future.展开更多
文摘Acrylamide(AA)is ubiquitous in packaging materials,paints,cosmetics,food and drinking water.However,AA has obvious neurotoxicity,reproductive toxicity and carcinogenicity,which seriously endangers human health.As one of precious traditional Chinese medicines,Gastrodia elata Bl.has been mainly used to lower blood pressure and resist myocardial ischemia.However,the antioxidant activity of Gastrodia elata Bl.on AA-induced DNA damage has not been reported.We found that AA(25,30 and 50 mg/kg)could cause DNA damage and the damage was time/dose dependent.After AA administration,Tail moment,micronuclei formation rate,8-OHdG and MDA level were significantly increased and SOD activity was decreased.Gastrodia elata Bl.decoction,gastrodin and vitamin C could reduce AA-induced DNA damage by decreasing AA-induced increment of tail moment and micronuclei formation rate.What’s more,Gastrodia elata Bl.decoction,gastrodin and vitamin C could lower 8-OHdG and MDA level and improve SOD activity.In conclusion,our research showed that Gastrodia elata Bl.decoction and gastrodin had protective effects on AA-induced DNA damage,and the potential protective mechanism might be related to anti-oxidative stress.
文摘A genomic DNA containing 5'-upstream region and complete open reading frame of a Gastrodia antifungal protein was isolated by screening of a genomic library from Gastrodia elata B1. To investigate the promoter activity, the 5'-flanking region - 1 157 lip upstream from the putative transcription start site was fused to the coding sequence of beta-glucuronidase (GUS) gene and transformed into Nicotiana tabacum. The strongest GUS activity was detected in the roots of transgenic tobacco, followed by stems. The leaves only showed a low GUS activity. Furthermore, the promoter established inducible expression pattern in transgenic tobacco upon fungus Trichoderma viride inoculation and jasmonic acid and salicylic acid treatments.
文摘为探究天麻(Gastrodia elata Bl.)质量评价的重要指标,本研究测定了27份陕西省汉中市天麻样品的外观性状、气味、颜色、含水量、浸出物和主要有效成分的含量,采用主成分分析(Principal Component Analysis,PCA)法确定其主要成分,结合熵权TOPSIS(Technique for Order Preference by Similarity to an Ideal Solution)法得出天麻质量评价体系中各评价指标的权重,并对天麻质量进行综合评价。结果表明汉中市宁强县、略阳县、勉县产出的天麻在块茎粗、螺环纹数、鲜重、干重、颜色明亮度和总色度值上有一定差异;水分、浸出物含量以略阳县最高;天麻素与对羟基苯甲醇总含量以宁强县最高,勉县所产天麻的天麻素与对羟基苯甲醇含量接近,其成分含量比例与其他县差异显著;此外,宁强县所产天麻的巴利森苷类成分含量最高。PCA熵权TOPSIS法评价结果显示,宁强县、略阳县天麻排名靠前,各项指标中颜色因子、巴利森苷类因子、气味因子权重较大,因此天麻质量评价应注重颜色、气味以及巴利森苷类成分含量。
基金The Foundation of Industrialization Demonstration and Promotion of Standardized Planting of Dominant Species of Gastrodia elata in Yingjing(General Service Station)the Foundation of Sichuan Sci ence and Technology Plan Project(Grant No.2019YFS0170).
文摘Gastrodia elata Bl.is a traditional Chinese herbal medicine with special pharmacological effects on headache and numbness.However,its wild sources are now in short supply because of over-excavation.A total of 14 endophytic fungi were isolated from Gastrodia elata Bl collected from Yinjing,in which 10 strains were identified,including Neonectria,Ascomycota,Fusarium,Oidiodendron and Dothiorella.All crude extracts showed some degrees of antioxidant activities by T-AOC method and DPPH methods,especially those from Oidiodendron GeR6 and Ascomycota sp.GeR2,which might be attributed due to their high phenolic contents.However,only Oidiodendron GeR6 contained gastrodin(39.2μg/g)as the host plant in the crude extracts.In conclusion,the notable activities of GeR6 indicated its medical use as a natural antioxidant agent,as well as substitutable use for Gastrodia elata Bl.in the future.
