镉超富集苋菜品种(Amaranthus mangostanus L.)的筛选

【目的】苋菜在中国的分布很广,品种资源丰富。通过筛选耐镉苋菜品种,发掘镉污染土壤植物修复资源。【方法】采用溶液培养方法,从来自不同生态区域的23个苋菜品种(Amaranthus mangostanus L.)中筛选出具有镉超富集能力的品种;采用赤红壤、黄棕壤、菜园土进行盆栽试验,研究苋菜在土壤Cd浓度分别为5、10、25mg·kg-1时,对镉污染土壤的修复潜力。【结果】在溶液Cd浓度3mg·L-1条件下培养,苋菜品种天星米地上部镉含量高达260mg·kg-1。在土壤Cd浓度25mg·kg-1条件下,苋菜天星米地上部镉浓度高达212mg·kg-1,富集系数达到8.5,地上部净化率达3.8%,各镉处理水平总生物量以及地上部生物量均未显著降低。【结论】苋菜天星米基本具备了镉超富集植物的特征,可用于镉污染土壤的生物修复。

不同镉积累型苋菜(Amaranthus mangostanus L.)根际低分子量有机酸与镉吸收的关系

【目的】研究低镉积累型苋菜紫背苋和高镉积累型苋菜天星米在镉胁迫下低分子量有机酸分泌特征及其与苋菜镉生物积累的关系。【方法】采用溶液培养法进行试验。【结果】随供镉浓度增加,紫背苋根、茎和叶生物量显著降低,而天星米生物量未发生明显改变。不同镉暴露条件下,苋菜分泌的有机酸总量表现为天星米远高于紫背苋,有机酸含量均表现为柠檬酸>苹果酸>乙酸>丙酸>丁酸,且以柠檬酸和苹果酸为主。两品种各有机酸数量占有机酸总量的比例基本一致。随供镉浓度增加,两个苋菜品种根分泌的各有机酸量增加,各有机酸分泌量的增幅均表现为天星米大大高于紫背苋,植株根、茎和叶镉含量与积累量也相应表现为天星米大大高于紫背苋。【结论】苋菜镉的生物积累与有机酸种类没有特异性关系,但与有机酸的总量有关。镉胁迫诱导根系分泌有机酸的数量,在引起两品种对镉吸收的差异方面起重要作用。

富钾植物籽粒苋(Amaranthus spp.)对土壤矿物钾的吸收利用研究

运用土培、石英砂培、有机酸释钾实验及矿物X衍射分析研究了不同基因型籽粒苋(Amaranthusspp.)对土壤矿物钾的吸收利用及其机制。结果表明,籽粒苋能有效地利用土壤和云母(黑云母和金云母)中的钾;籽粒苋品种R104、CX4对钾的吸收量高于一般型品种(CX77);籽粒苋根系能引起云母矿物向蛭石转化;籽粒苋根系分泌物中的草酸比一般有机酸具有更高的释放矿物中钾素的能力。

Effects of 1-MCP and Chitosan on Active Oxygen Metabolism and Quality of Fresh-cut Potato during Storage

[Objective] The research aimed to study the effects of 1-methylcyclopropene （1-MCP） and chitosan on active oxygen metabolism and quality of fresh-cut potato during storage. [Method] The fresh-cut potato slices were respectively treated with 1- MCP （2 μl/L）, chitosan or 1-MCP ＋ chitosan. During the storage period, the contents of superoxide anion （02）, malondialdehyde （MDA）, hydrogen dioxide （H202） and Vita- min C （Vc）, the activities of polyphenol oxidase （PPO）, peroxydase （POD） and super- oxide dismutase （SOD） as well as the respiratory rate of the fresh-cut potatoes in all the treatments were determined every day. The fresh-cut potato slices treated without any reagents were used as control. [Result] 1-MCP Treated could significantly de- creased the respiratory rate, PPO activity and the accumulations of 02 , H202 and MDA, increased the activities of SOD and POD and slowed down Vc content reduc- tion. On the contrary, potato treated with chitosan significantly inhibited the POD activi- ty in fresh-cut potato. Compared with the control, the combination of 1-MCP and chi- tosan showed a little but not significant better effects on potato preservation. [Conclu- sion] 1-MCP Treated showed the best effects on potato preservation.

Electron-Microscopic Study of Microsporogencsis in Male-sterile and Male-fertile Grain Amaranth(Amaranthus hypochondriacus L.)

A comparative study of microsporogenesis in male-sterile and male-fertile grain amaranth was conducted using electron-microscopy.The onset of microsporogenesis breakdown in male-sterile is at the mononuclear pollen stage following the release of microspores from tetrads.Abnormality in the behavior of degenerated tapetum was observed, which failed to envelope individual microspores after their release, leading to an abnormal vacuolation in the mononuclear pollen grains.As a result, the normal thickening of pollen wall could not occur and pollen grains could not engorge, causing male sterility.

Effects of Copper on the Photosynthesis and Oxidative Metabolism of Amaranthus tricolor Seedlings

The objective of the present study was to gain better insight into the physiological mechanisms on the effects of copper （Cu） on photosynthesis and active oxygen metabolism in three-colored amaranth plant （Amaranthus tricolor）. Three- colored amaranth seedlings were subjected to different Cu levels in soils during the entire experimental period. The parameters of growth, photosynthesis, mineral elements contents, and active oxygen metabolism were investigated using plant physiological methods. The results showed that 2.0 and 4.0 mmol Cu kg^-1 treatments decreased the whole plant biomass to 91 and 73% of the control, respectively. The net photosynthetic rate （Pn） and the stomatal conductance （gs） were similarly reduced in the third leaves of three-colored amaranth seedlings treated with 2.0 and 4.0 mmol Cu kg^-1 soil, respectively. None of the investigated Cu levels decreased the internal CO2 concentration （Ci）. The effect of Cu on the potential efficiency of photosystem Ⅱ （Fv/Fm） was negligible, whereas the effect of Cu on the PS Ⅱ quantum efficiency （ΦPS Ⅱ） after plant adaptation in actinic irradiation was more noticeable. On the other hand, decreases in water percentage, contents of photosynthetic pigments and mineral elements including Fe, K, and Mg, and significant increase in the Cu content were observed in the third leaves of Cu-treated plants. Superoxide dismutase （SOD） and peroxidase （POD） activities as well as the proline （Pro） content significantly increased in the third leaves of the three-colored amaranth seedlings treated with 2.0 and 4.0 mmol Cu kg^-1 soil, while catalase （CAT） and ascorbate peroxidase （APX） activities as well as the contents of carotenoid （Car）, glutathione （GSH）, and ascorbic acid （AsA） decreased, and accompanied by the increases in the contents of hydrogen peroxide （H2O2）, superoxide anion （O2^-）, and malondialdehyde （MDA）, and electrolyte leakage. As a result of the imbalance of active oxygen metabolism, Pn and ΦPS Ⅱ decreased, and peroxidization enhanced under levels of 2.0 and 4.0 mmol Cu kg^-1 soil. Finally, the growth of three-colored amaranth plant was significantly inhibited.

不同积累型苋菜(Amaranthus mangostanus L.)镉吸收转运特征差异性研究

【目的】比较高积累型苋菜品种Tianxingmi与低积累型苋菜品种Zibeixian在Cd胁迫下Cd吸收转运特征差异性,揭示苋菜品种Tianxingmi高积累镉的机理。【方法】采用水培试验,添加代谢抑制剂来研究两品种苋菜根系吸收Cd的主要途径。分别在30μmol/L Cd Cl2处理4 h、8 h、16 h、1 d和2 d采样测定,采用非损伤微测技术(Non-invasive micro-test technique,NMT),进行植物活体动态测试,调查、测量两品种苋菜根系Cd^(2+)离子流特征,比较了两品种对代谢抑制剂的反应。【结果】在30μmol/L Cd Cl2处理1 d后,Tianxingmi生物量达到最大值5.90 g/plant,是Zibeixian生物量的二倍;Tianxingmi根、茎和叶中Cd浓度分别为609、254和62.3mg/kg,分别是Zibeixian的1.4倍、1.9倍和1.6倍,地上部和全株的Cd累积量分别高达602.0、1308μg/plant;Tianxingmi富集系数(BCF)与转运系数(TF)分别为Zibeixian的2.1倍和1.5倍,这些结果两品种均表现出显著性差异(P<0.05)。NMT技术测定两品种根系Cd^(2+)离子流,通过扫描位点测定发现,距根尖0~300μm范围内两品种苋菜根系Cd^(2+)内流最强且差别最大,并在此做定点位点测定,发现Tianxingmi根系Cd^(2+)内流是Zibeixian根系Cd^(2+)内流的3.75倍,说明了两品种的富集特征与NMT结果一致。添加代谢抑制剂处理显著降低了高积累型Tianxingmi各器官中的Cd浓度,富集系数(BCF)与转运系数(TF),以及根系Cd^(2+)内流(P<0.05),主动吸收特征明显,而对低积累型Zibeixian的相关生理指标影响不大。【结论】两苋菜品种Cd吸收转运特征均表现出显著性差异。Tianxingmi具有更强的Cd吸收和向地上部转运与累积能力,并且根系对Cd的吸收与转运是主动需能的过程,共质体途径在Cd进入Tianxingmi根系并向地上部运输的过程中起着主要作用。

Screening of Amaranth Cultivars(Amaranthus mangostanus L.) for Cadmium Hyperaccumulation

The potential harm of soil cadmium pollution to ecological environment and human health has been increasingly widely concerned. Phytoremediation, as a kind of new and effective technology, has become an important method for cleaning up cadmium in contaminated sites. The amaranth （Amaranthus mangostanus L.） is widely distributed and has abundant varieties in China, its rapid growth and large biomass can be served as candidate for cadmium hyperaccumulators for phytoremediation. To obtain cadmium hyper-accumulator, Cd uptake in 23 amaranth cultivars from different ecological region was investigated under hydroponic culture condition. Meanwhile, pot experiment was established to probe phytoremediation potentiality of Cd contaminated soil by amaranth. Three treatment （Cd 5, 10, and 25 mg kg^-1） were imposed to red soil, yellow brown soil, and vegetable soil. The results showed that under hydroponic culture with Cd 3 mg L^-1, the cadmium concentration in the shoots of the cultivar Tianxingmi reached 260 mg kg^-1, and its total cadmium uptake was the highest among various cultivars. In the treatment Cd 25 mg kg^-1, the cadmium concentration in the shoots of the cultivar Tianxingmi reached 212 mg kg^-1, while bioaccumulation factor and shoot purification rate reached 8.50 and 3.8%, respectively. Further, the total biomass and shoot biomass were not decreased significantly under Cd exposure. These results suggested that cultivar Tianxingmi is a typical Cd hyperaccumulator, and can be expected to be used in phytoremediation of Cd contaminated soil.

Morphological Characterization of <i>Amaranthus palmeri</i>x <i>A. spinosus</i>Hybrids

The growth of clones of seven Amaranthus palmeri x A. spinosus hybrids was compared to type specimens of A. palmeri and A. spinosus. The hybrids came from the field where they were originally discovered and clones of the type specimens and hybrids were established under greenhouse conditions and used to compare growth rates. A. palmeri had the highest growth rate and A. spinosus the lowest growth rate based on height, node counts, and dry weight accumulation. A. palmeri also had the greatest number of days to flowering and A. spinosus the fewest. Hybrids had intermediary growth rates and days to flowering, but differed from each other with regard to sex identity. The hybrids were either dioecious like A. palmeri or, if monoecious, had patterns unlike A. spinosus. Spine length and texture also varied in hybrids and some were without spines. Hybrid 16Ci was short compared to all others and had succulent leaves and stems, which easily separated from the plant body. These hybridizations resulted in morphologically distinct types with acquisition of physical traits intermediate to the type specimens which may drive evolution of these species.

Effects of fresh-cut and storage on glucosinolates profile using broccoli as a case study

Glucosinolates(GLS) contribute to the unique flavour, nutrition, and plant defence of the Cruciferous vegetables. Understanding the GLS changes through postharvest processing is essential for defined preservation. In this study, four different fresh-cut types, whole flower(W),floret(F), quarterly cut floret(QF) and shredded floret(FS) of broccoli, were stored for 0, 1, 2 and 3 day(s) to explore GLS responses to postharvest treatments. As a result, seven GLS were identified, mainly including glucoraphanin(RAA), neoglucobrassicin(NEO), and glucobrassicin(GBC)and accounting for 52.69%, 20.12% and 14.99% of the total GLS(21.92 ± 0.48) μmol · g ^(-1 )DW, respectively. FS had the sharpest decrease in GLS after three days of storage(6.55 ± 0.37) μmol · g-1DW, while QF had the least(10.16 ± 0.33) μmol · g ^(-1 )DW. All GLS components decreased over storage, except for 4-methoxyglucobrassicin(4 ME) in FS and QF, suggesting its key role in serious wound defence. The results suggested certain postharvest approaches influenced the flavour and nutrition of broccoli.

Differential Response of Biomass Production and Nitrogen Uptake of Vegetable Amaranth to Two Types of Poultry Manure from Nigeria and Canada

Amaranthus cruentus is one of the sixty Amaranthus species of world. It is widely grown as a green leafy vegetable and for its grain in many tropical countries of Africa, Central and South American, Canada, Mexico and parts of Asia. Depleted soil fertility under intensive cultivation is a major limitation to production of the vegetable. The study investigated the effects of two types of poultry manure on biomass yield and nutrient uptake of Amaranth cruentus in a greenhouse at the University of Manitoba, Winnipeg, Manitoba, Canada in 2011. The experiment was a 2 × 3 × 3 factorial arranged into a Randomized Completely Block Design and replicated three times. The factors were two soil types, two sources of poultry manure and urea, and N rates at 0, 60 and 120 kg⋅N⋅ha−1. Nitrogen uptake by the plants that treated with poultry manure from Canada (PMC) and Urea was 172.5 vs. 171.4 mg⋅pot−1 and 169.5 vs. 163.7 mg⋅pot−1 respectively which were significantly greater than the poultry manure from Nigeria (PMN), 100.6 mg⋅pot−1 and 110.0 mg⋅pot−1 for Red River and Glenhope soil series, respectively. Nitrogen uptake was similar at the N rates of 60 and 120 kg⋅N⋅ha−1 (140.7 vs. 155.6 mg⋅pot−1) in the Red River soil, while N uptake at 120 kg⋅N⋅ha−1 was greater than at 60 kg⋅N⋅ha−1 in Glenhope soil. Shoot dry matter of the vegetable grown in soils treated with PMC and urea was significantly greater than from PMN in Red River soil (10.1, 9.6 and 6.7 g⋅pot−1) and in Glenhope soil (8.8, 8.1 and 6.4 g⋅pot−1). Phosphorus, K, Ca and Mg uptake at 60 and 120 kg⋅</

Genotypic Variations in Potassium Absorption and Utilization by Amaranthus spp.

The kinetics of K+ absorption and the utilization of both applied K and K in a calcareous alluvisol with low available K by different genotypes of grain amaranth (Amaranthus spp.), an ancient pseudo-cereal that produces a large biomass and a rich source of healthy nutrients and mineral elements, were studied by means of ion depletion technique and pot experiments. Grain amaranth had a high affinity for K+ uptake in comparison with corn and cotton. However, there were differences among Amaranthus spp. in K absorption and utilization. Some cultivars such as R104, CX-4, Du001, Hy015 and Hr029 which had a higher rate of K absorption and stronger affinity for K+ were typical varieties with high K-use efficiency (KUE). The results showed that high KUE cultivars grew quickly, possessed stronger ability to take up soil slowly available K and mineral K, and did not respond to K fertilization in the soil with low available K. Correspondingly, grain amaranth cultivars CX-77, Cr024, Vd001, Re003 and Sn003 were relatively low in KUE. Compared with high KUE cultivars, they took up more soil available K and both of their dry matter accumulation and K uptake responded to K applied significantly.

Bioassay and Characterization of Several Palmer Amaranth (<i>Amaranthus palmeri</i>) Biotypes with Varying Tolerances to Glyphosate

The wide distribution of Palmer amaranth (Amaranthus palmeri) in the southern US became a serious weed control problem prior to the extensive use of glyphosate-resistant crops. Currently glyphosate-resistant populations of Palmer amaranth occur in many areas of this geographic region creating an even more serious threat to crop production. Investigations were undertaken using four biotypes (one glyphosate-sensitive, one resistant from Georgia and two of unknown tolerance from Mississippi) of Palmer amaranth to assess bioassay techniques for the rapid detection and level of resistance in populations of this weed. These plants were characterized with respect to chlorophyll, betalain, and protein levels and immunological responses to an antibody of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) the target site of glyphosate. Only slight differences were found in four biotypes grown under greenhouse conditions regarding extractable soluble protein and chlorophyll content, but one biotype was found to be devoid of the red pigment, betalain. Measurement of early growth (seedling shoot elongation) of seedlings was a useful detection tool to determine glyphosate resistance. A leaf disc bioassay (using visual ratings and/or chlorophyll analysis) and an assay for shikimate accumulation were effective methods for determining herbicide resistance levels. The two unknown biotypes were found to be resistant to this herbicide. Some differences were found in the protein profiles of the biotypes, and western blots demonstrated a weak labeling of antibody in the glyphosate-sensitive biotype, whereas strong labeling occurred in the resistant plants. This latter point supports research by others, that increased copy number of the EPSPS gene (and increased EPSPS protein levels) is the resistance mechanism in this species. Results indicate the utility of certain bioassays for the determination of resistance and provide useful comparative information on the levels of inherent constituents among closely related plants.

Poly(amaranth) film based sensor for resolution of dopamine in the presence of uric acid:A voltammetric study

The electropolymerized film of amaranth was prepared on the surface of graphite pencil electrode（GPE） using cyclic voltammetric technique.This poly（amaranth） film coated electrode exhibited an excellent electrocatalytic activity towards the detection of dopamine（DA） in the presence of uric acid（UA） in 0.2 mol/L phosphate buffer solution at pH 7.0.The effect of interference study was carried out using differential pulse voltammetric technique.The poly（amaranth） modified GPE was applied for the detection of DA in dopamine injection with satisfactory results.

Interaction of the Bioherbicide <i>Myrothecium verrucaria</i>with Technical-Grade Glyphosate on Glyphosate-Susceptible and -Resistant Palmer Amaranth

Previously we found that a strain of Myrothecium verrucaria (MV) exhibited bioherbicidal activity against several important weeds, and that some commercial formulations of glyphosate applied with MV resulted in synergistic interactions that improved weed control efficacy. We also found that MV had bioherbicidal activity against glyphosate-resistant Palmer amaranth. We have also reported that some commercial formulations are inhibitory to MV. Our objectives were to test the effect of unformulated glyphosate (high purity, technical-grade glyphosate) alone and in combination with MV for bioherbicidal activity on glyphosate-susceptible and -resistant Palmer amaranth biotypes under greenhouse conditions and to examine technical-grade glyphosate on the growth of this bioherbicide. High purity glyphosate (without adjuvants/surfactants) was not toxic to MV growth and sporulation at concentrations up to 2.0 mM when grown on agar supplemented with the herbicide. Both biotypes were injured by MV and MV plus glyphosate treatments as early as 19 h after application (3 h after a dew period of 16 h). These injury effects increased and were more evident through the 6-day time course, when after 120 h the MV plus glyphosate treatment had killed all glyphosate-susceptible and -resistant plants. The interaction of glyphosate plus MV was synergistic toward the control of Palmer amaranth. Data strongly suggest that the active ingredient is responsible for the synergy previously found when this bioherbicide was combined with some commercial formulations of glyphosate. Results demonstrated that MV can control both glyphosate-resistant and -susceptible Palmer amaranth seedlings and act synergistically with high-purity glyphosate to provide improved weed control.

Effects of <i>Myrothecium verrucaria</i>on Two Glyphosate-Resistant <i>Amaranthus palmeri</i>Biotypes Differing in Betacyanin Content

Previously we found two biotypes of Amaranthus palmeri (Palmer amaranth) in a population of this economically important weed that were resistant to glyphosate but differed with respect to pigmentation. One biotype was typically red-pigmented (betacyanin) while the other was green, with no visual appearance of red hue on any plant part at any growth stage. We have also reported that a strain of Myrothecium verrucaria (MV) exhibited bioherbicidal activity against several important weeds including glyphosate-resistant Palmer amaranth. In greenhouse tests, MV was applied to these two biotypes (red and green) at two ages (3-week- and 6-week-old) and effects of this fungus monitored over a 5-day time course. Initial symptoms of MV (16 to 24 h after inoculation) were: epinastic curvature, wilting and development of lesions on leaves and stems. Generally, the younger plants tended to be more sensitive to MV than older plants. Bioherbicidal damage increased with time leading to necrosis and plant mortality and increasing disease progress. Severe loss of fresh weight occurred in both biotypes as compared to untreated plants. Results indicated that MV was effective on both biotypes, but effects on growth reduction and disease progression were more rapid and generally greater in the green biotype, suggesting that compounds responsible for red pigmentation may be more potent as defense against pathogen attack.

Kinetic and Thermodynamic Study for Fenton-Like Oxidation of Amaranth Red Dye

Oxidation by Fenton like reactions (Fe3+/H2O2) is economically process for destructive hazardous pollutants in waste water. The effects of different parameters such as, amaranth red dye, ferric chloride, hydrogen peroxide concentrations, pH value of solution, temperature and the presence of inorganic ions (carbonate, nitrate, chloride) on oxidative decolorization of amaranth were investigated. Amaranth degradation by (Fe3+/H2O2) reagent was found to follow first order kinetic model. Under optimum condition, pH = 2.6 and [FeCl3] = 3.75 × 10-4 mol·dm-3, the amaranth in aqueous solution with an initial concentration of 5 × 10-5 mol·dm-3 was degraded by 95% within 6 minutes. Increasing temperature in the range of 298 - 308 K increases the rate of dye degradation. Thermodynamic constants, ΔH*, ΔS* and ΔG* were evaluated. The results implied that the oxidation process was favorable and endothermic.

Effect of Storage Conditions on the Sensory Quality, Colour and Texture of Fresh-Cut Minimally Processed Cabbage with the Addition of Ascorbic Acid, Citric Acid and Calcium Chloride

The aim of this study was to investigate the effect of storage conditions on the sensory quality, colour and texture of fresh-cut cabbage during the addition of ascorbic acid, citric acid and calcium chloride. Ascorbic acid maintained the overall quality for 14 days at 0℃ and 7 days at 5℃;no difference, however, was observed regarding browning of cut surface compared to the control sample at both storage temperatures. Calcium chloride maintained the overall quality and cut surface browning for 14 days at both storage temperatures. It was also found that citric acid 1% can be used for minimally processed cabbage. Soaking with citric acid helped retain the color and increased the overall acceptance and organoleptic quality of fresh cut cabbage;it reduced browning of the cut surface and protected against formation of black specks. Citric acid treatment combined with low temperature storage (0℃) prolonged the shelf life of minimally processed cabbage for 22 days, time sufficient for acceptable marketing of the product. The lightness of minimally processed cabbage decreased linearly from 70.94 ± 6 to 63.8 ± 8.5 - 61.3 ± 8 units for the chemical treatments during 22 days of storage at 0℃. Hue angle values during storage time were also significantly influenced by chemical treatments mainly at 0℃.

Effects of Stable Chlorine Dioxide on Sterilization and Preservation of Fresh-cut Narrow-leaf Cattail

At present, chlorine dioxide has been widely used as a bactericide that may become an alternative to chlorine. In this study, fresh-cut narrow-leaf cattail was soaked with 45, 70 and 95 mg/L chlorine dioxide solution, respectively. Fresh-cut narrow-leaf cattail samples were collected regularly to determine changes in surface microbe amount, cellulose content, Vc content, reduction sugar content and sensory quality of narrow-leaf cattail, thus analyzing the effects of chlorine diox- ide on sterilization and preservation of fresh-cut narrow-leaf cattail. The results showed that all three concentrations of chlorine dioxide solution could significantly reduce the amount of microbes on the surface of fresh-cut narrow-leaf cattail and improve the edible safety of products. The initial sterilization efficiency was im- proved gradually as the concentration of chlorine dioxide increased. In addition, chlorine dioxide treatment postponed the increase of cellulose content of fresh-cut narrow-leaf cattail. However, chlorine dioxide oxidized Vc and reducing sugar, and its bleaching effect also exerted a certain impact on the sensory quality of fresh- cut narrow-leaf cattail. Based on comprehensive comparisons, 45 - 70 mg/L chlorine dioxide solution exerted the best effects on sterilization and preservation of fresh-cut narrow-leaf cattail. This study laid the foundation for the production and application of chlorine dioxide solution and promotion of rapid development of nar- row-leaf cattail industry.

Optimization of Preservation on Fresh-cut Yam under Simulated Cold-chain Process

[Objectives]This study was conducted to investigate the effect of compound fresh-keeping liquid on the browning and quality of fresh-cut yam during the cold chain process.[Methods]Under the simulated cold chain condition of 4℃,a uniform design(U6*(64))experiment was carried out to optimize the compound preservative formula,and a better compound preservative formula was obtained using the regression equation.[Results]The compound preservative formula was:AA 0.25%,CA 0.5%,Nisin 0.09%,GSH 0.15%.The compound fresh-keeping liquid effectively delayed the browning of fresh-cut yam and the accumulation of MDA,maintained the content of TSS,inhibited the activity of PAL and delayed the appearance of the maximum activity of POD and POD.[Conclusions]This study provides technical support for the actual extension of the shelf life of fresh-cut yam.