Epidemiology of cercarial stage of trematodes in freshwater snails from Chiang Mai province,Thailand

Objective:To investigate the epidemiological situation of cercarial trematodes infection in freshwater snails from different water resources in Chiang Mai province,Thailand.Methods:The snail specimens were collected from 13 districts of Chiang Mai province during April 2008 to February 2012.The prevalence of cercarial infection in snails was investigated using the crushing method.The drawing was done with the help of a camera lucida for the morphological study.Results:A total of 2479 snail individuals were collected and classified into 7 families,11 genera,and 14 species,Among them,8 snails species were found to be infected with an overall prevalence of 17.27%(428/2479),which infected with nine groups of cercariae;gymnocephalous cercaria,strigea cercaria,megalurous cercaria,monostome cercaria,parapleurolophocercous cercaria(Haplorchis cercaria),pleurolophocercous cercaria,furcocercous cercaria(Transversotrema cercaria),xiphidiocercaria,and virgulate cercaria.The parapleurolophocercous cercaria was found to be the dominant type among the cercarial infection in the snails(64.25%).Conclusions:The various species of snails found in the research location act as the intermediate hosts for the high prevalence of parasitic infection of many species of mammals.This work will provide new information on both the distribution and first intermediate host of trematodes.

Chromosome-level genome assembly of the freshwater snail Bellamya purificata(Caenogastropoda)

DEAR EDITOR,Bellamya purificata(Gastropoda:Caenogastropoda;Architaenioglossa;Viviparidae:Bellamyinae;Sinotaia),a homotypic synonym of S.purificata,is widely distributed in freshwater habitats in Asia.It is an economically important edible snail and plays a vital function in freshwater wetland ecology.However,genomic resources for this snail are lacking and no reference genome has been released.In this study,we constructed the first chromosome-level genome of B.purificata using PacBio long-read sequencing and high-throughput chromosome conformation capture(Hi-C)technology.

Epidemiological situation and molecular identification of cercarial stage in freshwater snails in Chao-Phraya Basin, Central Thailand

Objective: To investigate the prevalence of cercarial trematode infection in snails and to examine the reconstruction of the phylogenetic relationship to explain the molecular system of cercarial stage trematodes to estimate the infection rate of in the definite host from the Chao-Phraya Basin.Methods: The snails were collected from 10 provinces of the Chao-Phraya Basin,Thailand by stratified sampling method. The snails were examined for cercarial infection by the crushing method. All DNA specimens were amplified with internal transcribed spacer 3(ITS3) and ITS4 primer based on PCR technique. The sequence data were aligned and used to reconstruct the phylogenetic tree by unweighted pair-group method with arithmetic means with 10 000 bootstraps.Results: The overall rate of cercarial infection was found to be 5.90%(122/2 067). Snails in the family Thiaridae were found to be in the highest prevalence followed by Lymnaeidae, Bithyniidae, Planorbidae, Viviparidae, and Ampullariidae, respectively, while the Buccinidae family(Clea helena) did not reveal any infections. The frequently found species of cercariae were parapleurolophocercous cercariae, cercariae and megarulous cercariae. The monophyletic tree separated the snails into five groups comprised of Heterophyidae, Strigeidae, Lecithodendriidae, Philophthalmidae and Echinostomatidae using the sequence of Angiostrongylus cantonensis as an out-group.Conclusions: This study was the first to report on cercarial infection in the Chao-Phraya Basin, Thailand. This revealed that a high variety of freshwater snails were infected by cercariae stage trematodes with a high prevalence. The sequence data of ITS2 can be used to investigate the phylogenetic relationships of trematodes at the family level and in each clade of different families separated by the definitive hosts.

Prevalence of cercarial infections in freshwater snails and morphological and molecular identification and phylogenetic trends of trematodes

Objective:To investigate the prevalence of cercarial infections in freshwater snails from several water sources in Nakhon Nayok,Nonthaburi,and Pathum Thani provinces of Central Thailand,and to reconstruct a phylogenetic tree for improved understanding of the relationships in the cercarial stage.Methods:The snail specimens were collected from 34 total sampling sites and investigated for cercarial infections using the crushing method.The cercarial specimens were classified and used for the phylogenetic tree analysis using the Internal Transcribed Spacer 2(ITS2).Results:A total of 1921 snail specimens were classified into five families and seven species.The results showed that four snail species were identified as intermediate hosts of the larval stages of trematodes,with an overall prevalence of infection of 2.45%(47/1921).The infected snail specimens included five groups of the cercarial type:cercariaeum cercariae,echinostome cercaria,megalurous cercaria,parapleurolophocercous cercaria,and xiphidiocercariae.This is particularly true of xiphidiocercariae,which was found to be the dominant type among cercarial infections in bithyniid snails by approximately 38.00%.With regard to molecular identification,the phylogenetic tree was reconstructed using the neighbor-joining method with 10000 bootstraps and separated the trematodes into three clades:Echinostomatoidea,Microphalloidea and Opisthorchioidea.Conclusions:The study reveals a high prevalence of cercarial infection for each cercarial type and maturation into a definite trematode genus and delineates morphological characteristics and evolutionary trends among each larval trematode in Nakhon Nayok,Nonthaburi and Pathum Thani provinces.In addition,the ITS2 sequence data of cercariae could be used to examine classification of these species at the family level.

Water Quality Evaluation Index System for Freshwater Aquaculture Pond

[Objective] The aim was to establish the water quality evaluation index system for freshwater aquaculture pond.[Method] The expert survey,DELPHI,field research and other methods were used.Based on the analysis of factors influencing water quality of freshwater aquaculture pond,and the importance degree of 14 factors influencing water quality of freshwater aquaculture pond was ordered.Then,five factors were selected as index to establish the water quality evaluation index system of freshwater aquaculture with the determination for thresholds of these factors.[Result] The importance degree of water quality of freshwater aquaculture pond showed an order of Dissolved oxygenpHPhytoplankton biomassSecchi depthTotal NitrogenZooplankton biomassWater temperatureBiochemical Oxygen DemandWater colorSalinityTotal hardness;according to the value of importance degree,the dissolved oxygen,pH,secchi depth,phytoplankton biomass and total nitrogen these five factors were selected as the index system of water quality evaluation of pond.The level of water quality of freshwater aquaculture pond was divided into five,and the tolerance range of fish to each index was obtained by expert observation.[Conclusion] This study had provided a scientific basis for water quality evaluation system of freshwater aquaculture pond.

COQ10B在食管鳞癌中的表达及其调控Snail表达促进食管鳞癌细胞增殖与侵袭

目的:探讨COQ10B对食管鳞癌细胞增殖、侵袭能力的影响及可能的分子机制。方法:通过TIMER2.0数据库分析COQ10B基因在各种肿瘤和相邻正常组织之间的表达水平。免疫组织化学检测COQ10B在食管鳞癌组织中的表达水平并进行临床病理特征分析及生存分析;利用慢病毒构建COQ10B敲减稳转株及Snail过表达稳转株,qRT-PCR和Western blot方法检测转染效率。MTT法、Celigo细胞计数及Transwell侵袭小室检测COQ10B对KYSE150细胞系增殖和侵袭能力的影响。通过GEPIA2数据库分析COQ10B表达水平与Snail的相关性;Western blot检测敲减COQ10B对Snail蛋白表达的影响。通过拯救实验分析COQ10B是否通过正调控Snail促进KYSE150细胞系增殖和侵袭能力。结果:COQ10B在ESCC组织及细胞系中均为高表达水平,且表达水平与临床病理特征病理分级相关。COQ10B与ESCC患者预后相关,高表达COQ10B的ESCC患者预后明显较差,且发现性别、肿瘤TNM分期是影响ESCC预后的独立危险因素。敲减COQ10B可降低ESCC细胞增殖及侵袭能力。COQ10B表达水平与Snail呈正相关,并通过正调控Snail促进KYSE150细胞系增殖和侵袭能力。结论:COQ10B通过正向调控Snail基因表达促进食管鳞癌增殖与侵袭。

结肠腺癌组织中Cripto-1蛋白和锌指转录因子Snail与基质金属蛋白酶-9的表达及临床意义

目的研究结肠腺癌组织中畸胎瘤衍生生长因子-1(Cripto-1)、锌指转录因子(Snail)和基质金属蛋白酶-9(MMP-9)的表达情况及临床病理特征。方法回顾性分析2020年1月至2021年8月于韶关市粤北人民医院胃肠外科92例行手术切除结肠腺癌患者的临床资料,手术切除标本92个。另选取癌旁不典型增生组织56例和正常结肠组织35例作为对照。采用免疫组化检测并比较Cripto-1、Snail和MMP-9在结肠腺癌组织、不典型增生和正常结肠黏膜组织中的表达情况,采用Spearman相关分析Cripto-1、Snail和MMP-9与结肠腺癌临床病理特征的相关性。结果结肠腺癌、不典型增生、正常结肠组织的CR-1、Snail和MMP-9阳性率比较差异有统计学意义(P<0.05);结肠腺癌及不典型增生组织中CR-1、Snail和MMP-9阳性率均高于正常结肠组织,结肠腺癌组织中Snail和MMP-9阳性率均高于不典型增生组织,差异均有统计学意义(P<0.05);结肠腺癌组织与不典型增生组织中CR-1阳性率比较差异无统计学意义。肿瘤的浸润深度、临床TNM分期及淋巴结转移情况不同的患者Cripto-1、Snail和MMP-9在结肠腺癌组织中的表达阳性率比较差异有统计学意义(P<0.05)。肿瘤浸润T3~T4、临床分期Ⅲ~Ⅳ期、有淋巴结转移的患者结肠腺癌组织中CR-l、Snail和MMP-9的表达阳性率均高于肿瘤浸润T1~T2、临床分期Ⅰ~Ⅱ期和无淋巴结转移的患者,差异有统计学意义(P<0.05)。结肠腺癌组织中CR-1表达与Snail和MMP-9的表达呈正相关(r>0,P<0.05),MMP-9表达与Snail表达亦呈正相关(r>0,P<0.05)。结论Cripto-1、Snail和MMP-9在结肠癌的浸润和转移中起协同调节作用。

Sensitivity of ENSO Variability to Pacific Freshwater Flux Adjustment in the Community Earth System Model

The effects of freshwater flux （FWF） on modulating ENSO have been of great interest in recent years. Large FWF bias is evident in Coupled General Circulation Models （CGCMs）, especially over the tropical Pacific where large precipitation bias exists due to the so-called ＂double ITCZ＂ problem. By applying an empirical correction to FWF over the tropical Pacific, the sensitivity of ENSO variability is investigated using the new version （version 1.0） of the NCAR＇s Community Earth System Model （CESM1.0）, which tends to overestimate the interannual variability of ENSO accompanied by large FWF into the ocean. In response to a small adjustment of FWF, interannual variability in CESM1.0 is reduced significantly, with the amplitude of FWF being reduced due to the applied adjustment part whose sign is always opposite to that of the original FWF field. Furthermore, it is illustrated that the interannual variability of precipitation weakens as a response to the reduced interannual variability of SST. Process analysis indicates that the interannual variability of SST is damped through a reduced FWF-salt-density-mixing-SST feedback, and also through a reduced SST-wind-thermocline feedback. These results highlight the importance of FWF in modulating ENSO, and thus should be adequately taken into account to improve the simulation of FWF in order to reduce the bias of ENSO simulations by CESM.

Effects of elevated CO_2 concentration and nitrogen supply on biomass and active carbon of freshwater marsh after two growing seasons in Sanjiang Plain, Northeast China

An experiments were carried out with treatments differing in nitrogen supply （0, 5 and 15 g N/m^2） and CO2 levels （350 and 700 μmol/mol） using OTC （open top chamber） equipment to investigate the biomass of Calamagrostis angustifolia and soil active carbon contents after two years. The results showed that elevated CO2 concentration increased the biomass of C. angustifolia and the magnitude of response varied with each growth period. Elevated CO2 concentration has increased aboveground biomass by 16.7% and 17.6% during the jointing and heading periods and only 3.5% and 9.4% during dough and maturity periods. The increases in belowground biomass due to CO2 elevation was 26.5%, 34.0% and 28.7% during the heading, dough and maturity periods, respectively. The responses of biomass to enhanced CO2 concentrations are differed in N levels. Both the increase of aboveground biomass and belowground biomass were greater under high level of N supply （15 g N/m^2）. Elevated CO2 concentration also increased the allocation of biomass and carbon in root. Under elevated CO2 concentration, the average values of active carbon tended to increase. The increases of soil active soil contents followed the sequence of microbial biomass carbon （10.6%） 〉 dissolved organic carbon （7.5%） 〉 labile oxidable carbon （6.6%） 〉 carbohydrate carbon （4.1%）. Stepwise regressions indicated there were significant correlations between the soil active carbon contents and plant biomass. Particularly, microbial biomass carbon, labile oxidable carbon and carbohydrate carbon were found to be correlated with belowground biomass, while dissolved organic carbon has correlation with aboveground biomass. Therefore, increased biomass was regarded as the main driving force for the increase in soil active organic carbon under elevated CO2 concentration.

Quantitative Analysis of the Feedback Induced by the Freshwater Flux in the Tropical Pacific Using CMIP5

Freshwater flux （FWF） directly affects sea surface salinity （SSS） and hence modulates sea surface temperature （SST） in the tropical Pacific. This paper quantifies a positive correlation between FWF and SST using observations and simulations of the fifth phase of the Coupled Model Intercomparison Project （CMIP5） to analyze the interannual variability in the tropical Pacific. Comparisons among the displacements of FWF, SSS and SST interannual variabilities illustrate that a large FWF variability is located in the west-central equatorial Pacific, covarying with a large SSS variability, whereas a large SST variability is located in the eastern equatorial Pacific. Most CMIP5 models can reproduce the fact that FWF leads to positive feedback to SST through an SSS anomaly as observed. However, the difference in each model＇s performance results from different simulation capabilities of the CMIP5 models in the magnitudes and positions of the interannual variabilities, including the mixed layer depth and the buoyancy flux in the equatorial Pacific. SSS anomalies simulated from the CMIP5 multi-model are sensitive to FWF interannual anomalies, which can lead to differences in feedback to interannual SST variabilities. The relationships among the FWF, SSS and SST interannual variabilities can be derived using linear quantitative measures from observations and the CMIP5 multi-model simulations. A 1 mm d-1 FWF anomaly corresponds to an SSS anomaly of nearly 0.12 psu in the western tropical Pacific and a 0.11℃ SST anomaly in the eastern tropical Pacific.

Ecological service assessment of human-dominated freshwater ecosystem with a case study in Yangzhou Prefecture, China

Freshwater ecosystems provide a host of services to humanity. These services are now rapidly being lost, not least because of the inability of making the impacts measurable. To overcome this obstacle, assessment frameworks for freshwater ecosystem services are needed. A simple water equivalent framework to assess the ecological services provided by freshwater ecosystems was developed in this study. It translated the occupation of freshwater ecosystem services into biologically freshwater volumes and then compares this consumption to the freshwater throughput, that is, the ecological capacity available in this region. In this way, we use the example of Yangzhou Prefecture, to account the main categories of human occupation of water ecosystem services. The result showed that there is a huge gap between the consumption and the supply of freshwater ecosystem services. This must encourage local government to make land-use and water management decisions both economically rational and environmentally sound.

Simulation of Salinity Variability and the Related Freshwater Flux Forcing in the Tropical Pacific: An Evaluation Using the Beijing Normal University Earth System Model(BNU-ESM)

The climatology and interannual variability of sea surface salinity （SSS） and freshwater flux （FWF） in the equatorial Pacific are analyzed and evaluated using simulations from the Beijing Normal University Earth System Model （BNU-ESM）. The simulated annual climatology and interannual variations of SSS, FWF, mixed layer depth （MLD）, and buoyancy flux agree with those observed in the equatorial Pacific. The relationships among the interannual anomaly fields simulated by BNU-ESM are analyzed to illustrate the climate feedbacks induced by FWF in the tropical Pacific. The largest interannual variations of SSS and FWF are located in the western-central equatorial Pacific. A positive FWF feedback effect on sea surface temperature （SST） in the equatorial Pacific is identified. As a response to El Nino-Southern Oscillation （ENSO）, the interannual variation of FWF induces ocean processes which, in turn, enhance ENSO. During El Nino, a positive FWF anomaly in the western-central Pacific （an indication of increased precipitation rates） acts to enhance a negative salinity anomaly and a negative surface ocean density anomaly, leading to stable stratification in the upper ocean. Hence, the vertical mixing and entrainment of subsurface water into the mixed layer are reduced, and the associated E1 Nino is enhanced. Related to this positive feedback, the simulated FWF bias is clearly reflected in SSS and SST simulations, with a positive FWF perturbation into the ocean corresponding to a low SSS and a small surface ocean density in the western-central equatorial Pacific warm pool.

On a unique behavior of freshwater bacteria

Magnetic sensitivity has been found in bacteria from freshwater sites around Pavia, Italy.

UPPER OCEAN RESPONSE TO SURFACE MOMENTUM AND FRESHWATER FLUXES IN THE WESTERN PACIFIC WARM POOL

A series of numerical experiments are carried out to study the tropical upper ocean response to combined momentum and buoyancy forcing, with emphasis on the three-dimensional thermohaline structure in the western Pacific warm pool. In response to climatological winds, heat fluxes and freshwater input, the model is able to simulate the salient dynamic and hydrographic features of the tropical Pacific Ocean and their seasonal variability. In response to idealized episodic westerly wind bursts and rainfall, the simulated upper ocean conditions compare favorably with available observations, thus enabling us to identify important physical processes involved. Local forcing, vertical mixing and meridional advection dominate the salt and heat budgets in the warm pool on short time scales, but it is necessary to include the saline water coming from the east with the South Equatorial Current to close the salt budget on seasonal and longer time scales. Strong westerly wind bursts generate a swift eastward equatorial jet and a pair of meridional circulation cells with convergence at the equator. This results in an equatorward advection of relatively fresh water from the north and a depression of the thermocline at the equator. Heavy rainfall reduces the surface mixed layer depth by creating a shallow halocline, thus trapping the momentum and heat inputs near the surface. The remote influences of the episodic momentum and buoyancy fluxes are very different. Westerly wind bursts can generate large downstream disturbances in both dynamic and thermal fields through the propagation of equatorial waves, while the effect of rainfall is mostly confined to the forcing area.

Bioaccumulation and depuration of chromium in the selected organs and whole body tissues of freshwater fish Cirrhinus mrigala individually and in binary solutions with nickel

Contamination of aquatic ecosystems with heavy metals has been receiving increased worldwide attention due to their harmful effects on human health and other organisms in the environment. Most of the studies dealing with toxic effects of metals deal with single metal species, while the aquatic organisms are typically exposed to mixtures of metals. Hence, in order to provide data supporting the usefulness of freshwater fish as indicators of heavy metal pollution, it has been proposed in the present study to investigate the bioaccumulation and depuration of chromium in the selected organs of freshwater fingerlings Cirrhinus mrigala, individually and in binary solutions with nickel. The results show that the kidney is a target organ for chromium accumulation, which implies that it is also the ＂critical＂ organ for toxic symptoms. The results further show that accumulation of nickel in all the tissues of C. mrigala is higher than that of chromium. In addition, the metal accumulations of the binary mixtures of chromium and nickel are substantially higher than those of the individual metals, indicating synergistic interactions between the two metals. Theoretically the simplest explanation for an additive joint action of toxicants in a mixture is that they act in a qualitatively similar way. The observed data suggest that C. mrigala could be suitable monitoring organisms to study the bioavailability of water-bound metals in freshwater habitats.

Variation of freshwater components in the Canada Basin during 1967–2010

As a conservative tracer, oxygen isotopes in seawater are widely used for water mass analysis, along with temperature and salinity. In this study, seawater oxygen-18 datasets in the Canada Basin during 1967-2010 were obtained from the four cruises of the Chinese National Arctic Research Expedition （1999, 2003, 2008, and 2010） and the NASA database. Fractions of sea ice meltwater and river runoffwere determined from the salinity-5180 system. Our results showed that the river runoff decreased from the south to the north in the Canada Basin. The enhanced amount of river runoff observed in the southern Canada Basin may originate from the Mackenzie River, transported by the Beaufort Gyre. The river runoff component showed maximum fractions during 1967-1969, 1978-1979, 1984-1985, 1993-1994, and 2008-2010, indicating the refresh time of the river runoffwas 5.0-16.0 a in the Canada Basin. The temporal variation of the river runoffwas related to the change of the Arctic Oscillation （AO） index, suggesting the freshwater stored in the Canada Basin was affected by surface sea ice drift and water mass movement driven by atmospheric circulation.

Molecular Fingerprints of Soil Organic Matter in a Typical Freshwater Wetland in Northeast China

Natural wetlands are known to store huge amounts of organic carbon in their soils. Despite the importance of this storage,uncertainties remain about the molecular characteristics of soil organic matter(SOM), a key factor governing the stability of soil organic carbon(SOC). In this study, the molecular fingerprints of SOM in a typical freshwater wetland in Northeast China were investigated using pyrolysis gas-chromatography/mass-spectrometry technology(Py-GC/MS). Results indicated that the SOC, total nitrogen(TN),and total sulfur contents of the cores varied between 16.88% and 45.83%, 0.93% and 2.82%, and 1.09% and 3.79%, respectively. The bulk δ^13C and δ^15N varied over a range of 9.85‰, between –26.85‰ and –17.00‰, and between –0.126‰ and 1.002‰, respectively. A total of 134 different pyrolytic products were identified, and they were grouped into alkyl(including n-alkanes(C:0) and n-alkenes(C:1),aliphatics(Al), aromatics(Ar), lignin(Lg), nitrogen-containing compounds(Nc), polycyclic aromatic hydrocarbons(PAHs), phenols(Phs), polysaccharides(Ps), and sulfur-containing compounds(Sc). On average, Phs moieties accounted for roughly 24.11% peak areas of the total pyrolysis products, followed by Lg(19.27%), alkyl(18.96%), other aliphatics(12.39%), Nc compounds(8.08%), Ps(6.49%), aromatics(6.32%), Sc(3.26%), and PAHs(1.12%). Soil organic matter from wetlands had more Phs and Lg and less Nc moieties in pyrolytic products than soil organic matters from forests, lake sediments, pastures, and farmland.δ^13 C distribution patterns implied more C3 plant-derived soil organic matter, but the vegetation was in succession to C4 plant from C3 plant. Significant negative correlations between Lg or Ps proportions and C3 plant proportions were observed. Multiple linear analyses implied that the Ar and Al components had negative effects on SOC. Alkyl and Ar could facilitate ratios between SOC and total nitrogen(C/N), while Al plays the opposite role. Al was positively related to the ratio of dissolved organic carbon(DOC) to SOC. In summary, SOM of wetlands might characterize by more Phs and lignin and less Nc moieties in pyrolytic products. The use of Pyrolysis gas-chromatography/mass-spectrometry(Py-GC/MS) technology provided detailed information on the molecular characteristics of SOM from a typical freshwater wetland.

Modulation of El Nio-Southern Oscillation by Freshwater Flux and Salinity Variability in the Tropical Pacific

The E1 Nifio-Southern Oscillation （ENSO） is emphasized the roles of wind stress and heat flux environmental forcing to the ocean; its effect and modulated by many factors; most previous studies have in the tropical Pacific. Freshwater flux （FWF） is another the related ocean salinity variability in the ENSO region have been of increased interest recently. Currently, accurate quantifications of the FWF roles in the climate remain challenging; the related observations and coupled ocean-atmosphere modeling involve large elements of uncertainty. In this study, we utilized satellite-based data to represent FWF-induced feedback in the tropical Pacific climate system; we then incorporated these data into a hybrid coupled ocean-atmosphere model （HCM） to quantify its effects on ENSO. A new mechanism was revealed by which interannual FWF forcing modulates ENSO in a significant way. As a direct forcing, FWF exerts a significant influence on the ocean through sea surface salinity （SSS） and buoyancy flux （QB） in the western-central tropical Pacific. The SSS perturbations directly induced by ENSO-related interannual FWF variability affect the stability and mixing in the upper ocean. At the same time, the ENSO-induced FWF has a compensating effect on heat flux, acting to reduce interannual Qs variability during ENSO cycles. These FWF-induced processes in the ocean tend to modulate the vertical mixing and entrainment in the upper ocean, enhancing cooling during La Nifia and enhancing warming during E1 Nifio, respectively. The interannual FWF forcing-induced positive feedback acts to enhance ENSO amplitude and lengthen its time scales in the tropical Pacific coupled climate system.

Influences of Freshwater from Major Rivers on Global Ocean Circulation and Temperatures in the MIT Ocean General Circulation Model

Responses of global ocean circulation and temperature to freshwater runoff from major rivers were studied by blocking regional runoff in the global ocean general circulation model （OGCM） developed at the Massachusetts Institute of Technology. Runoff into the tropical Atlantic, the western North Pacific, and the Bay of Bengal and northern Arabian Sea were selectively blocked. The blocking of river runoff first resulted in a salinity increase near the river mouths （2 practical salinity units）. The saltier and, therefore, denser water was then transported to higher latitudes in the North Atlantic, North Pacific, and southern Indian Ocean by the mean currents. The subsequent density contrasts between northern and southern hemispheric oceans resulted in changes in major ocean currents. These anomalous ocean currents lead to significant temperature changes （I^C -2~C） by the resulting anomalous heat transports. The current and temperature anomalies created by the blocked river runoff propagated from one ocean basin to others via coastal and equatorial Kelvin waves. This study suggests that river runoff may be playing an important role in oceanic salinity, temperature, and circulations; and that partially or fully blocking major rivers to divert freshwater for societal purposes might significantly change ocean salinity, circulations, temperature, and atmospheric climate. Further studies are necessary to assess the role of river runoff in the coupled atmosphere-ocean system.

Phosphorus accumulation and eutrophication in feed-supply freshwater fishponds

The rapid growth and intensification of freshwater fishery can cause imbalances between phosphorus （P） input in feed and its output in produce. This aquaculture can result in enriching exogenous P in fishponds and, consequently, accelerates the process of eutrophication. To assess relations among input, accumulation, release of P and as a consequence degrading water quality in terms of chlorophyll-a （Chl-a） in freshwater fishponds, fourteen fishponds with feed supply, nine fishponds without feed supply, and five nonfish ponds in Shaoxing Plain, southeast China were selected for comparing P accumulation in their waters and surface sediments. Surface sediment samples were collected from each pond to evaluate their total P, water soluble P, Olsen P, algal available P, and P fractions. Water samples were also collected from the ponds to measure concentrations of dissolved P and Chl-a. Total P in the sediments ranged from 0.88 to 1.73 g/kg in the fishponds with feed supply, that in the non-fish ponds ranged from 0.47 to 0.86 g/kg. Organic P, accounted for 23% to 60% of total P in the sediments, was an important P fraction and increased linearly with increasing organic matter. Long-term application of feeds resulted in increased P availability in the bottom sediments and degradation of water quality in the freshwater fishponds. Compared with non-fish ponds, sediments from the feed-supplied fishponds contained considerably higher Olsen P, algal available P, and water soluble P. Higher proportions of the labile P （NH4Cl-P） and potentially labile P （NaOH-IP） were also found in the sediments from the fishponds. High solubility of P in the sediments resulted in elevation of P and chlorophyll-a concentration in the pond water. The dissolved P concentration in the pond water increased in the order of non-fish ponds （12μg/L） 〈 fishponds without feed supply （24 μg/L） 〈 fishponds with feed supply （66 μg/L）. Linear correlations between concentrations of total P, Olsen-P, algal available P, water-soluble P and P concentration in saturation extracts in the sediments and dissolved P in the pond water indicated that there was a buffering action of the sediment constituents on the dissolved P.