Fritillaria cirrhosa D.Don(Liliaceae)is an endangered perennial bulbous plant and its dry bulb is a valuable med-icinal material with antitussive and expectorant effects.Nevertheless,lack of resources and expensive pr...Fritillaria cirrhosa D.Don(Liliaceae)is an endangered perennial bulbous plant and its dry bulb is a valuable med-icinal material with antitussive and expectorant effects.Nevertheless,lack of resources and expensive prices make it difficult to meet clinical needs.This study presents a regeneration system aimed at overcoming the challenge of inadequate supply in F.cirrhosa,focusing on:(1)callus induction,(2)bulblets and adventitious bud induction,and(3)artificial seed production.Callus development was achieved in 84.93%on Murashige and Skoog(MS)medium fortified with 1.0 mg·L^(-1) picloram.The optimal medium for callus differentiation into regenerated bulb-lets was MS medium supplemented with 1.0 mg·L^(-1)6-benzyladenine(6-BA)and 0.2 mg·L^(-1)α-naphthaleneacetic acid(NAA).Subsequently,bulblets and adventitious buds were induced from regenerated bulblet sections cul-tured on MS medium fortified with 0.3 mg·L^(-1)6-BA+1.0 mg·L^(-1)2,4-dichlorophenoxyacetic acid(2,4-D),2.0 mg·L^(-1)6-BA+0.5 mg·L^(-1),and the induction rates were 88.17%and 84.24%,respectively.The regenerated bulblets were transplanted into a substrate of humus soil,river sand,and pearlite(1:1:1)after low-temperature treatment.The germination rate was 42.80%after culture for 30 days.Regenerated bulblets were used for encap-sulations in liquid MS medium containing 3%sucrose(w/v)+0.5 mg·L^(-1) NAA+2.0 mg·L^(-1)6-BA+3%sodium alginate(w/v)with a 10 min exposure to 2%CaCl_(2).Under non-aseptic conditions,the germination rate reached 81.67%,while the rooting rate was 20.56%after 45 days.The capsule added 1.0 g·L^(-1) carbendazim and 1.0 g·L^(-1) activated carbon was the best component of artificial seeds.This study successfully established an efficient regen-eration system for the rapid propagation of F.cirrhosa,involving in vitro bulblet regeneration and artificial seed production.This method introduces a novel approach for efficient breeding and germplasm preservation,making it suitable for large-scale industrial resource production.展开更多
Fritillariae Cirrhosae Bulbus is a well-known traditional Chine medicinal herb. However, the demand for this herb is leading to over-collection and its decline in the wild. This study aims to investigate the ecologica...Fritillariae Cirrhosae Bulbus is a well-known traditional Chine medicinal herb. However, the demand for this herb is leading to over-collection and its decline in the wild. This study aims to investigate the ecological conditions of Fritillaria cirrhosa under the influence of human disturbance, biotic species interactions and climatic conditions. We established a total of 78 plots at 14 sites of F. cirrhosa in its distribution center, the Hengduan Mountains area. At each site, we estimated the abundance of F. cirrhosa at different distances from roads. The diameter and height of F. cirrhosa fruit and bulbs were measured and compared to underground bulb depth. We then analyzed the effects of environmental conditions and human disturbance on the abundance of F. cirrhosa using variance partitioning. We found that(1)abundance of F. cirrhosa and their underground bulb depth showed a significant linear increase with the distance from the main road;(2) the diameter/height of fruits and the diameter/height of fruits/bulbs showed significantly different responses to the human disturbance;(3) the community associates,climate and spatial conditions can explain 58%, 22% and 27%, respectively, of the variance in the F. cirrhosa abundance. These results highlight the fact that human disturbance and biotic factors have a great influence on the survival of F. cirrhosa, even more than climate conditions.展开更多
Protected areas are generally regarded as a power tool to conserve biodiversity.Nonetheless,few protected areas could address three crucial problems simultaneously,namely funding,public participation and rural living....Protected areas are generally regarded as a power tool to conserve biodiversity.Nonetheless,few protected areas could address three crucial problems simultaneously,namely funding,public participation and rural living.Here,we introduced a new protective approach,Natural Fostering,which integrated herbal medicine production with community conservation.The principles of Natural Fostering adopted species–species interaction at community level.Most effective chemical components of herbal medicine are derived from such interaction.Fritillaria cirrhosa was selected as an economic botany,one of herbal medicines,to carry out Natural Fostering.Community habitats,herbal medicine production,funding and income of local family were investigated to verify the feasibility of Natural Fostering for biodiversity.We found the density of plant populations and the annual average personal income of rural people increased.F.cirrhosa production could provide sufficient funds for sustainable conservation.Local people gradually changed their life style of wild collection and overgrazing,instead of herbal medicine production.The fostering area set up a good sustainable economic cycle.Natural Fostering can be presented as an effective and pragmatic way to conserve biological diversity and sustainable utilization of traditional medicinal resources.展开更多
基于数据挖掘川贝母治疗肺系疾病的核心中药和基于网络药理学探讨“川贝母-人参”治疗肺系疾病之支气管哮喘的作用机制。通过数据处理软件,利用统计学方法对所收集的含贝母肺系疾病中药组方进行频数统计、关联规则分析和聚类分析。并利...基于数据挖掘川贝母治疗肺系疾病的核心中药和基于网络药理学探讨“川贝母-人参”治疗肺系疾病之支气管哮喘的作用机制。通过数据处理软件,利用统计学方法对所收集的含贝母肺系疾病中药组方进行频数统计、关联规则分析和聚类分析。并利用网络药理学知识对核心药对治疗肺系典型疾病支气管哮喘进行靶点收集筛选、基因本体论(gene ontology,GO)富集分析和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)富集分析,最后利用分子对接验证。共筛选出含贝母处方420个,涉及中药463味;高频药物有甘草、杏仁等;聚类分析可得8类;高频核心二联药物组合有“川贝母-阿胶”“川贝母-人参”等,三联药物组合包括“川贝母-天冬-麦冬”“川贝母-天冬-甘草”等,四联药物组合“川贝母-桑白皮-桔梗-甘草”“川贝母-桑白皮-杏仁-甘草”等;由于阿胶为动物药材,则分析“川贝母-人参”药对治疗作为治疗支气管哮喘这一典型肺系疾病的核心药对,其中Hub靶点包括“AKT1”“PTGS2”等;核心活性成分包括“beta-sitosterol”“kaempferol”等;预测跟脂质和动脉粥样硬化、流体剪切应力和动脉粥样硬化、弓形虫病、糖尿病并发症中的高级糖基化终末产物-受体(advanced glycation end products-receptor for AGE,AGE-RAGE)信号通路有关,在分子对接中高频活性药物成分Fumarine与疾病靶点的PTGS2最为紧密。基于统计学方法和网络药理学技术,川贝母常与人参等配伍使用治疗肺系疾病,且药物的活性成分会利用相关AKT1等靶点和脂质和动脉粥样硬化等通路起到治疗作用;本研究揭示了含川贝母治疗肺系疾病方剂潜在的一些规律,为进一步开放川贝母组方药物提供了思路。展开更多
基金funded by the National Key Research and Development Program of China(2018YFC1706101)the Science and Technology Program of Sichuan Province,China(2021YFS0045).
文摘Fritillaria cirrhosa D.Don(Liliaceae)is an endangered perennial bulbous plant and its dry bulb is a valuable med-icinal material with antitussive and expectorant effects.Nevertheless,lack of resources and expensive prices make it difficult to meet clinical needs.This study presents a regeneration system aimed at overcoming the challenge of inadequate supply in F.cirrhosa,focusing on:(1)callus induction,(2)bulblets and adventitious bud induction,and(3)artificial seed production.Callus development was achieved in 84.93%on Murashige and Skoog(MS)medium fortified with 1.0 mg·L^(-1) picloram.The optimal medium for callus differentiation into regenerated bulb-lets was MS medium supplemented with 1.0 mg·L^(-1)6-benzyladenine(6-BA)and 0.2 mg·L^(-1)α-naphthaleneacetic acid(NAA).Subsequently,bulblets and adventitious buds were induced from regenerated bulblet sections cul-tured on MS medium fortified with 0.3 mg·L^(-1)6-BA+1.0 mg·L^(-1)2,4-dichlorophenoxyacetic acid(2,4-D),2.0 mg·L^(-1)6-BA+0.5 mg·L^(-1),and the induction rates were 88.17%and 84.24%,respectively.The regenerated bulblets were transplanted into a substrate of humus soil,river sand,and pearlite(1:1:1)after low-temperature treatment.The germination rate was 42.80%after culture for 30 days.Regenerated bulblets were used for encap-sulations in liquid MS medium containing 3%sucrose(w/v)+0.5 mg·L^(-1) NAA+2.0 mg·L^(-1)6-BA+3%sodium alginate(w/v)with a 10 min exposure to 2%CaCl_(2).Under non-aseptic conditions,the germination rate reached 81.67%,while the rooting rate was 20.56%after 45 days.The capsule added 1.0 g·L^(-1) carbendazim and 1.0 g·L^(-1) activated carbon was the best component of artificial seeds.This study successfully established an efficient regen-eration system for the rapid propagation of F.cirrhosa,involving in vitro bulblet regeneration and artificial seed production.This method introduces a novel approach for efficient breeding and germplasm preservation,making it suitable for large-scale industrial resource production.
基金supported by Yunnan Environmental Protection Special Fund 2013, Grant No. 214203the National Natural Science Foundation of China (NSFC) Grant No. 31560063Key Disciplines (Ecology) Project of Yunnan Education Department
文摘Fritillariae Cirrhosae Bulbus is a well-known traditional Chine medicinal herb. However, the demand for this herb is leading to over-collection and its decline in the wild. This study aims to investigate the ecological conditions of Fritillaria cirrhosa under the influence of human disturbance, biotic species interactions and climatic conditions. We established a total of 78 plots at 14 sites of F. cirrhosa in its distribution center, the Hengduan Mountains area. At each site, we estimated the abundance of F. cirrhosa at different distances from roads. The diameter and height of F. cirrhosa fruit and bulbs were measured and compared to underground bulb depth. We then analyzed the effects of environmental conditions and human disturbance on the abundance of F. cirrhosa using variance partitioning. We found that(1)abundance of F. cirrhosa and their underground bulb depth showed a significant linear increase with the distance from the main road;(2) the diameter/height of fruits and the diameter/height of fruits/bulbs showed significantly different responses to the human disturbance;(3) the community associates,climate and spatial conditions can explain 58%, 22% and 27%, respectively, of the variance in the F. cirrhosa abundance. These results highlight the fact that human disturbance and biotic factors have a great influence on the survival of F. cirrhosa, even more than climate conditions.
基金The study was funded by the National Natural Science Foundation of China(Grant No.30572324 and 81130069).
文摘Protected areas are generally regarded as a power tool to conserve biodiversity.Nonetheless,few protected areas could address three crucial problems simultaneously,namely funding,public participation and rural living.Here,we introduced a new protective approach,Natural Fostering,which integrated herbal medicine production with community conservation.The principles of Natural Fostering adopted species–species interaction at community level.Most effective chemical components of herbal medicine are derived from such interaction.Fritillaria cirrhosa was selected as an economic botany,one of herbal medicines,to carry out Natural Fostering.Community habitats,herbal medicine production,funding and income of local family were investigated to verify the feasibility of Natural Fostering for biodiversity.We found the density of plant populations and the annual average personal income of rural people increased.F.cirrhosa production could provide sufficient funds for sustainable conservation.Local people gradually changed their life style of wild collection and overgrazing,instead of herbal medicine production.The fostering area set up a good sustainable economic cycle.Natural Fostering can be presented as an effective and pragmatic way to conserve biological diversity and sustainable utilization of traditional medicinal resources.
文摘基于数据挖掘川贝母治疗肺系疾病的核心中药和基于网络药理学探讨“川贝母-人参”治疗肺系疾病之支气管哮喘的作用机制。通过数据处理软件,利用统计学方法对所收集的含贝母肺系疾病中药组方进行频数统计、关联规则分析和聚类分析。并利用网络药理学知识对核心药对治疗肺系典型疾病支气管哮喘进行靶点收集筛选、基因本体论(gene ontology,GO)富集分析和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)富集分析,最后利用分子对接验证。共筛选出含贝母处方420个,涉及中药463味;高频药物有甘草、杏仁等;聚类分析可得8类;高频核心二联药物组合有“川贝母-阿胶”“川贝母-人参”等,三联药物组合包括“川贝母-天冬-麦冬”“川贝母-天冬-甘草”等,四联药物组合“川贝母-桑白皮-桔梗-甘草”“川贝母-桑白皮-杏仁-甘草”等;由于阿胶为动物药材,则分析“川贝母-人参”药对治疗作为治疗支气管哮喘这一典型肺系疾病的核心药对,其中Hub靶点包括“AKT1”“PTGS2”等;核心活性成分包括“beta-sitosterol”“kaempferol”等;预测跟脂质和动脉粥样硬化、流体剪切应力和动脉粥样硬化、弓形虫病、糖尿病并发症中的高级糖基化终末产物-受体(advanced glycation end products-receptor for AGE,AGE-RAGE)信号通路有关,在分子对接中高频活性药物成分Fumarine与疾病靶点的PTGS2最为紧密。基于统计学方法和网络药理学技术,川贝母常与人参等配伍使用治疗肺系疾病,且药物的活性成分会利用相关AKT1等靶点和脂质和动脉粥样硬化等通路起到治疗作用;本研究揭示了含川贝母治疗肺系疾病方剂潜在的一些规律,为进一步开放川贝母组方药物提供了思路。
