Health benefits of Grifola frondosa polysaccharide on intestinal microbiota in type 2 diabetic mice

Grifola frondosa polysaccharide(GFP)as the natural compounds have been reported to exert diverse bioactivities.The aim of this study was to investigate the regulatory effects of GFP on intestinal microbiota in type 2 diabetic mice.The changes of microbiota in faeces of the diabetic mice upon high fat diet were determined and the V3 region of the 16S rRNA was sequenced by Illumina MiSeq high-throughput sequencing platform.Eighty operational taxonomic unit were identified to be shared by all samples,and the quality and richness of sequencing were assessed via rarefaction and rank abundance curves.The diversity of gut flora was slightly improved in diabetic mice after GFP treatment.The composition and relative abundance of gut microbiota at phylum and genus levels were altered by GFP.The abundance of Robinsoniella,Flavonifractor,Anaerotruncus,and Desulfvibrio were found to concentrate on diabetic mice through LEfSe analysis.Furthermore,Alloprevotella and Burkholderia had strong relevancy with other gut flora.Based on the findings,GFP might be a desired candidate on ameliorating the intestinal unbalance in diabetes.

Effect of diets on the feeding behavior and physiological properties of suspension-feeding sea cucumber Cucumaria frondosa

The suspension-feeding sea cucumber Cucumaria frondosa has become commercially important in recent years.Finding proper diets is the first important step for intensive aquaculture of this sea cucumber.In this study,adult C.frondosa were exposed to one of the following diet treatments:control(no diet provided),two powdered seaweeds(Ascophyllum nodosum and Saccharina latissima),a commercially available microalgal diet(shellfish diet)and natural seston.The effects of diets on the feeding behavior and physiological properties of sea cucumbers were investigated after a 5-week rearing period.Results show that sea cucumbers fed with shellfish diet exhibited a significantly higher tentacle insertion rate(1.80±0.20 insertion/min)than these fed with seaweed powders,and there was no significant different between the two groups fed by seaweed powders.No significant difference was found on the fecal production rate among the feeding groups.The minimum oxygen consumption rate was observed in the control group(5.76±0.99μg O2/(g·h)),which is significantly lower than individuals fed with A.nodosum,shellfish diet,and natural seston;however,no significant difference was shown between those of control and S.latissimi groups.The maximum ammonium excretion rate was found in the A.nodosum group(0.03±0.01μmol/(g·h)),which is significantly higher than other groups.The minimum O/N ratio was observed in the A.nodosum group(14.57±1.04),which is significantly lower than the S.latissima,shellfish diet,and natural seston groups.Individuals fed with seaweed powders had similar physiological properties with these fed with microalgae diet and natural seston,indicating that A.nodosum and S.latissima can be explored as promising diets for intensive aquaculture of C.frondosa.

Optimization of Conditions for Extracting Intracellular Polysaccharide with Anti-tumor Activity from Grifola frondosa

The Box-Behnken design was used to determine the optimal time,temperature and solvent∶solid ratio for extracting intracellular polysaccharide from G.frondosa mycelium produced under submerged culture conditions.Application of response surface methodology predicted a maximum polysaccharide extraction yield of 7.28% using an extraction time of 2.24 h,an extraction temperature of 92.53 ℃,and a water∶mycelium ratio of 35.76∶1.Under these conditions,the actual yield was 7.15%.Both low(200 mg/kg/d)and high(500 mg/kg/d)doses of G.frondosa polysaccharide,and positive controls administered 20 mg/kg/d cyclophosphamide,significantly inhibited the growth of S180 tumors with inhibition values of 61.5%,44.42% and 73.8%,respectively compared with negative controls administered physiological saline.

灰树花菌(Griflola frondosa)子实体原基转录组测序及分析

采用Illumina测序平台对灰树花菌(Griflola frondosa)原基进行转录组测序,获得了大量转录组信息.结显示果,测序得到38189个非冗余Unigene,在Nr数据库中有31454个Unigene被注释;与KEGG数据库比对,共有1832个Unigene被注释,分属20条生物通路;在COG数据库中注释可划分为25类;与GO数据库进行注释可分为分子功能、细胞组分和生物过程3个主类和60个二级亚类.

灰树花水溶物对EV71病毒的抑制作用

【目的】探讨灰树花水溶物(GFWE)的化学成分及其对EV71病毒的抑制作用,为促进灰树花综合利用、提高其经济附加值,以及开发预防和治疗EV71病毒感染的功能性食品提供依据。【方法】采用色谱法测定了GFWE的主要化学成分组成、多糖组成和分子质量。基于体外细胞培养采用CCK-8法确定了GFWE的安全上样量;以EV71病毒感染Vero细胞为模型,探究GFWE对EV71病毒的抑制率及对Vero细胞形态的影响,并采用荧光定量PCR检测了EV71病毒衣壳蛋白(VP1)编码基因mRNA的表达水平,初步评价了GFWE对EV71病毒的抑制作用。【结果】GFWE主要由低分子质量多糖、氨基酸、肽及其衍生物组成。GFWE富含的13种氨基酸中包含7种必需氨基酸;其多糖由葡萄糖、半乳糖、盐酸氨基葡萄糖和古罗糖醛酸4种单糖组成,物质的量比为0.907∶0.038∶0.029∶0.026,重均分子质量分别为15.67、10.10、6.60 ku和<1.00 ku。GFWE表现出较高的抗EV71病毒活性,100、200μg·mL^(-1) GFWE对EV71病毒的抑制率分别为91.32%、91.83%,25~400μg·mL^(-1) GFWE能显著降低EV71 VP1 mRNA的表达水平。【结论】GFWE主要由低分子质量多糖、氨基酸、肽及其衍生物组成,具有较高的抗EV71病毒活性,能够抑制感染EV71病毒的Vero细胞中VP1的合成。

基于IL-6/JAK2/STAT3信号通路探讨灰树花提取物对溃疡性结肠炎大鼠结肠组织炎症反应的影响

目的:探讨灰树花提取物通过调控白细胞介素6(IL-6)/蛋白酪氨酸激酶2(JAK2)/信号转导和激活因子3(STAT3)信号通路,对溃疡性结肠炎(UC)大鼠结肠组织炎症反应的影响及作用机制。方法:将40只SD大鼠随机分为空白对照组、UC模型组、灰树花治疗组、西药治疗组、联合治疗组,每组8只。采用3%DSS自由饮用法7 d建立UC大鼠模型后,治疗组分别灌胃灰树花提取物10 mg/(kg·d)、柳氮磺吡啶0.3 g/(kg·d)及等量两种药物,连续14 d。实验期间观察大鼠一般状态,计算疾病活动指数(DAI)评分;采用HE染色法观察大鼠结肠组织病理改变;Western blot检测大鼠结肠组织IL-6、JAK2、STAT3、pSTAT3蛋白表达水平;ELISA检测大鼠血清中IL-6含量;免疫组化法测定大鼠结肠组织中IL-6R、MPO蛋白含量及表达情况。结果:与空白对照组比较,UC模型组大鼠一般状态欠佳,DAI评分升高,HE染色可见明显的组织黏膜损伤与炎症细胞浸润;结肠组织IL-6、JAK2、STAT3、p-STAT3蛋白表达水平及IL-6R、MPO含量均显著升高(P<0.01);血清中IL-6含量显著升高(P<0.01),差异有统计学意义。与UC模型组比较,各治疗组大鼠的一般状态均有所改善,DAI评分降低,HE染色可见黏膜损伤有不同程度改善,偶见炎症细胞浸润;结肠组织IL-6、JAK2、STAT3、p-STAT3蛋白表达水平显著降低(P<0.01),结肠组织IL-6R和MPO含量及血清中IL-6含量均明显减少(P<0.01或P<0.05),差异有统计学意义。结论:灰树花提取物可通过调控IL-6/JAK2/STAT3信号通路相关因子表达,减轻UC大鼠结肠组织炎症反应。

橙足海参多肽的制备工艺优化及其抗氧化活性研究

以橙足海参(Cucumaria frondosa)为原料,通过水解度、可溶性多肽含量、DPPH自由基清除率、ABTS自由基清除率、总还原力和铁离子还原能力(ferric reducing antioxidant power,FRAP)等理化指标的分析,结合单因素实验和响应面实验,探究了复合酶酶解法制备海参多肽的最佳工艺参数。利用切向流超滤技术对海参酶解液进行分离,考察了不同分子量段的海参多肽在体外消化前后的抗氧化活性。结果表明,最佳工艺参数为时间5.0 h、温度50.0℃和pH值7.0,在此条件下测得ABTS自由基清除率为38.43%。当分子量<3000 Da时,DPPH自由基清除率IC 50达到2.76 mg/mL,ABTS自由基清除率IC 50达到2.61 mg/mL,总还原力为0.31,FRAP为0.16μmol/mL Fe^(2+)当量。同时,体外消化结果显示,海参多肽的ABTS自由基清除率上升,DPPH自由基清除率、总还原力和FRAP下降,但是仍具有较高的抗氧化活性。实验结果表明,海参多肽在消化前后均具有良好的抗氧化活性,为其功能性食品组分的添加和开发提供了理论依据。

Expression of the Grifola frondosa Trehalose Synthase Gene and Improvement of Drought-Tolerance in Sugarcane （Saccharum officinarum L.）

Trehalose Is a nonreduclng dlsaccharlde of glucose that functions as a protectant In the stabilization of blologlcal structures and enhances stress tolerance to abiotic stresses in organisms. We report here the expression of a Grlfola frondosa trehalose synthase （TSase） gene for Improving drought tolerance In sugarcane （Saccharum offlclnarum L.）. The expression of the transgene was under the control of two tandem copies of the CaMV35S promoter and transferred Into sugarcane by Agrobacterium tumefaciens EHA105. The transgenlc plants accumulated high levels of trehalose, up to 8.805-12.863 mg/g fresh weight, whereas It was present at undetectable level in nontransgenlc plants. It has been reported that transgenlc plants transformed with Escherlchla coil TPS （trehalose-6-phosphatesynthase） and/or TPP （trehalose-6-phosphate phosphatase） are severely stunted and have root morphologlc alterations. Interestingly, our transgenlc sugarcane plants had no obvious morphological changes and no growth Inhibition in the field. Trehalose accumulation in 35S-35S：TSase plants resulted In In- creased drought tolerance, as shown by the drought and the drought physiological Indexes, such as the rate of bound water/free water, plasma membrane permeability, malondlaldehyde content, chlorophyll a and b contents, and activity of SOD and POD of the excised leaves. These results suggest that transgenlc plants transformed with the TSase gene can accumulate high levels of trehalose and have enhanced tolerance to drought.

Immunomodulatory activity of macromolecular polysaccharide isolated from Grifola frondosa

The present study was designed to evaluate the immune-modulating effects of the polysaccharide from Grifola frondosa(GFP) by using mouse peritoneal macrophage and cytoxan(CTX) induced immunosuppression models. Our results from the phagocytotic and mononuclear phagocytic system function assays showed that GFP-A(one component from GFP) stimulated the phagocytosis of the phagocytes. The splenocyte proliferation assay showed that GFP-A acted the effect combing Con A or LPS in splenocyte proliferation. The results showed that GFP-A increased indices of thymus and spleen, the levels of LDH and ACP in the spleen, the m RNA levels of IL-1β, IL-2, IL-6 and IFN-γ in splenocyte. And GFP-A also significantly increased the expression of CD4^+ and CD8^+ splenic T lymphocytes, which were suppressed by the CTX in peripheral blood. In conclusion, our results indicate that the GFP-A is involved in immunomodulatory effects leading to its modulatory effects on immunosuppression.

Genetic Transformation of Tobacco with the Trehalose Synthase Gene from Grifola frondosa Fr. Enhances the Resistance to Drought and Salt in Tobacco

Trehalose is a non-reducing disaccharide of glucose that functions as a protectant in the stabilization of biological structures and enhances the tolerance of organisms to abiotic stress. In the present study, we report on the expression of the Grifola frondosa Fr. trehalose synthase (TSase) gene for manipulating abiotic stress tolerance in tobacco (Nicotiana tabaccum L.). The expression of the transgene was under the control of two tandem copies of the CaMV3 5 S promoter and was transferred into tobacco by Agrobacterium tumefaciens EHA105. Compared with non-transgenic plants, transgenic plants were able to accumulate high levels of products of trehalose, which were increased up to 2.126–2.556 mg/g FW, although levels were undetectable in non-transgenic plants. This level of trehalose in transgenic plants was 400-fold higher than that of transgenic tobacco plants cotransformed with Escherichia coli TPS and TPP on independent expression cassettes, twofold higher than that of transgenic rice plants transformed with a bi functional fusion gene (TPSP) of the trehalose-6-phosphate (T-6-P) synthase (TPS) and T-6-P phosphatase (TPP) of E. coli, and 12-fold higher than that of transgenic tobacco plants transformed the yeast TPS1 gene. It has been reported that transgenic plants with E. coli TPS and/or TPP were severely stunted and had morphological alterations of their roots. Interestingly, our transgenic plants have obvious morphological changes, including thick and deep-coloured leaves, but show no growth inhibition; moreover, these morphological changes can restore to normal type in T2 progenies. Trehalose accumulation in 35S–35S:TSase plants resulted in increased tolerance to drought and salt, as shown by the results of tests on drought, salt tolerance, and drought physiological indices, such as water content in excised leaves, malondialdehyde content, chlorophyll a and b contents, and the activity of superoxide dismutase and peroxidase in excised leaves. These results suggest that transgenic plants transformed with the TSase gene can accumulate high levels of trehalose and have enhanced tolerance to drought and salt.

响应面法优化栗蘑多糖提取工艺及其抗氧化活性研究

为优化栗蘑多糖提取工艺,以栗蘑子实体为研究对象,通过硫酸-苯酚滴定法,结合紫外分光光度法测定栗蘑多糖提取率。在单因素多水平实验的基础上,采用响应面法,利用Design-Expert软件,选取提取时间、提取温度、提取液料比3个因素,设置三水平实验方案优化提取工艺。结果表明,栗蘑多糖最佳提取工艺为提取时间1.9 h、提取温度80℃、提取液料比37∶1(mL/g),此条件下栗蘑多糖提取率为14.45%。抗氧化活性研究结果表明,栗蘑多糖对DPPH自由基和羟基自由基都具有较好的清除能力,在一定范围内,抗氧化活性随着多糖浓度的增加而提高。

灰树花多糖的促进合成及其对酒精性胃损伤的缓解作用

目的探究鱼腥草(Houttuynia cordata Thunb,HCT)醇提物对灰树花多糖(Grifolafrondosa polysaccharide,GFP)产量的影响及对慢性酒精性胃组织损伤的缓解作用。方法基于HCT醇提物与GF发酵制得灰树花多糖(命名为HCTGFP),将108只KM雄性小鼠随机分为9组,除空白组外,各处理组每周二、四、六给药1 h后以5 mL/kg剂量灌胃50%食品级乙醇,连续给药4周;测定小鼠胃组织中氧化应激指标、抗炎标记物、炎症因子含量及其基因表达;测定小鼠血清中炎症因子含量并观察小鼠胃组织病理变化;采用16sRNA高通量测序法检测小鼠肠道菌群的变化。结果HCT提高了37.6%胞外多糖产量(P<0.01),且产生的HCTGFP能够有效增加超氧化物歧化酶活性、谷胱甘肽含量、降低丙二醛含量,提高机体抗氧化水平;有效抑制环氧合酶-2、白细胞介素-1β、白细胞介素-6和一氧化氮合酶基因的表达,缓解体内炎症反应。在门水平上,与模型组相比,HCTGFPH显著回调了F/B比值(P<0.05),显著增加了拟杆菌门丰度(P<0.05)以及显著降低了厚壁菌门、脱硫菌门与变形菌门的相对丰度(P<0.05);在属水平上,摄入HCTGFP回调了拟普雷沃氏菌属(Alloprevotella)的相对丰度,抑制了毛螺菌科属(Lachnospiraceae_NK4A136_group)、脱硫弧菌属(Desulfovibrio)的相对丰度,有效调节小鼠肠道菌群稳态。结论HCT能够显著提高GFP产量以及提升其调控小鼠胃组织氧化应激、炎症因子基因表达、肠道微生物菌群的效能,对慢性酒精性胃损伤起到更好的保护作用。

灰树花多肽-锌螯合物对孕期缺锌鼠后代的改善作用

灰树花多肽-锌螯合物(Grifola frondosa Polypeptide Zinc Chelate,GPs-Zn)是灰树花多肽(Grifola frondosa Polypeptide,GPs)和锌离子的螯合产物,利用电镜扫描、X-射线粉末衍射技术、红外光谱和紫外光谱等技术对GPs-Zn进行结构分析;通过构建ICR母鼠缺锌模型,进一步探究GPs-Zn对孕期缺锌鼠后代的影响。表征结果表明,GPs和锌离子螯合成一种新的物质;红外光谱对比图发现肽链上的氨基和羧基都参与了锌离子的配位反应;紫外光谱图发现,GPs-Zn中多肽的羰基原子和锌离子结合,诱导紫外光谱的红移,表明GPs与锌离子结合形成GPs-Zn。动物实验表明:GPs-Zn可将缺锌仔鼠胸腺指数提高78.69%(雌鼠)和87.55%(雄鼠);脾脏指数提高40.28%(雌鼠)和43.22%(雄鼠);体质量提高89.98%(雌鼠)和88.30%(雄鼠);且血清的超氧化物歧化酶(SOD)活力提高了108.07%(雌鼠)和26.16%(雄鼠);锌浓度提高14.74%(雌鼠)和29.33%(雄鼠);碱性磷酸酶(AKP)水平降低52.28%(雌鼠)和62.48%(雄鼠)。综上可知,GPs-Zn对孕期缺锌鼠后代仔鼠的胸腺指数、脾脏指数、超氧化物歧化酶活力、锌浓度和碱性磷酸酶水平有一定改善作用。

灰树花可溶固形物提取工艺探究

在热水提取条件下,研究灰树花可溶固形物提取工艺。通过考查子实体形态、干燥方式对灰树花可溶固形物提取率的影响,再以提取温度、提取时间、料液比、浸洗次数开展单因素试验及正交试验,筛选最佳热水提取工艺。结果表明灰树花菌盖部分的开片程度越高,越利于可溶固形物的溶出。影响灰树花可溶固形物提取率高低的干燥方式依次为鼓风干燥>冷冻干燥>微波干燥。灰树花可溶固形物的最佳热水提取工艺为,鼓风干燥预处理,提取温度70℃,料液比1∶20,提取时间2.5 h,pH自然,浸洗1次。最佳工艺下灰树花可溶固形物提取率达到54.74%。

灰树花工厂化栽培技术

灰树花工厂化栽培具有生产周期短、占地少、产品品质优、管理方便等优势。介绍适合浙江地区的灰树花工厂化栽培技术,包括设施要求、品种选择、培养基质配方、原料准备、制棒灭菌、冷却接种、发菌管理、开孔、上架、出菇管理、采收及病虫害防控等。

灰树花多糖对胃溃疡大鼠肠激素及氧化因子的影响

探讨灰树花多糖对胃溃疡大鼠胃泌素(gastrin,GAS)、生长抑素(somatostatin,SS)、胃动素(motilin,MTL)、超氧化物歧化酶(Superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)和谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)含量的影响。选取SPF级健康SD雄性大鼠70只,随机分为空白组10只、实验组60只,实验组采用“乙酸灌胃法”制作大鼠胃溃疡模型,1周后将造模成功的50只大鼠随机分为模型组、奥美拉唑组和灰树花多糖低、中、高剂量组,每组各10只。奥美拉唑组灌胃125 mg/(kg·d)、灰树花多糖组大鼠灌胃低剂量0.243 g/(kg·d)、中剂量0.486 g/(kg·d)、高剂量0.972 g/(kg·d),1次/d,连续8周,观察各组大鼠状态,记录体质量和进食量、溃疡面积、溃疡指数;采用ELISA法检测大鼠血清GAS(pg/mL)、SS(pg/mL)、MTL(pg/mL)水平;采用胺法测定SOD的活力、 TBA比色法测定MDA、 GSH-Px的含量。模型组大鼠较空白对照组GAS、SS、MDA升高(P<0.05或P<0.01),MTL、SOD、GSH-Px降低(P<0.05或P<0.01),药物干涉组随着灰树花多糖浓度的提高SOD、GSH-Px升高(P<0.05或P<0.01),GAS、MTL、SS、MDA指标降低(P<0.05或P<0.01),灰树花多糖具有降低胃肠激素分泌以及提高抗氧化酶(SOD、GSH-Px)含量的作用。

灰树花多糖对小鼠抗疲劳作用的影响

以雄性小鼠为研究对象,口服喂食剂量分别为100、200、400 mg/(kg·d)的灰树花多糖(GFP),通过强迫游泳试验评价小鼠的耐力,探究GFP不同剂量对小鼠抗疲劳作用的影响。结果表明:不同剂量GFP显著减少了小鼠悬浮时间(P<0.05),小鼠脑干中去甲肾上腺素、5-羟色胺、5-羟基吲哚乙酸、多巴胺含量以及血清中血清氨、无机磷酸盐含量、乳酸和血清肌酸激酶活性与阴性对照组相比均呈现下降趋势(P<0.05),肌糖原含量、肌肉乳酸脱氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶活性与阴性对照组相比显著提高(P<0.05)。GFP具有提高小鼠抗疲劳的作用,可用于抗疲劳产品的开发。

灰树花多糖调节脂肪代谢作用的研究进展

灰树花是一种食、药兼用的蕈菌,含有多种对人类有益活性成分,其多糖是一种功能性多糖,具有独特的结构和多种生理活性,可以改善高热量饮食所造成的脂肪代谢异常,在调节脂肪代谢作用中具有毒性小、作用广和靶点多等特点。脂肪代谢紊乱是血液和组织中脂质及其代谢产物发生异常,由高脂饮食、缺乏运动以及代谢综合症等因素诱发其发生和发展,严重危害人类健康。脂肪代谢障碍会导致血脂异常、动脉硬化和肥胖等代谢相关疾病,严重影响身体健康甚至危及生命。近年来,许多天然多糖可以作为益生元,通过调控关键基因的表达以及肠道微生物群来预防代谢紊乱。灰树花多糖可以作为功能性食品用于治疗或预防高脂血症、动脉硬化以及肥胖等疾病。该研究将从调节甘油三酯和脂肪酸、胆固醇以及肠道菌群结构等方面探讨灰树花多糖对脂肪代谢的影响。

灰树花不同浓度醇提物的活性成分分析及抗氧化、抗EV71病毒的作用研究

【目的】探究灰树花适宜的乙醇提取浓度,分析不同乙醇浓度提取对灰树花活性成分及抗氧化、抗EV71病毒活性的影响,促进灰树花的开发利用。【方法】分别采用55%、70%和95%的乙醇溶液作为提取溶剂对灰树花子实体进行超声波辅助提取,测定不同浓度乙醇提取物主要活性成分含量并对其抗氧化、抗EV71病毒能力进行相关性分析。【结果】不同乙醇浓度提取物活性成分含量存在差异,灰树花95%乙醇提取物(GFE95)中多酚和三萜含量最高分别为0.042%和1.82%,灰树花55%乙醇提取物(GFE55)中多糖和蛋白质含量最高,分别为33.08%和17.45%。GFE55、灰树花70%乙醇提取物(GFE70)和GFE95具有不同程度的抗氧化和EV71病毒抑制作用。其中,GFE95的抗氧化能力最好,对2,2-联苯基-1-苦基肼基(DPPH)、2,2′-联氨双二胺盐(ABTS+)、超氧阴离子(O_(2)^(-))和过氧化氢(H_(2)O_(2))自由基的清除能力的IC50值分别为0.7427、0.7414、0.902、0.4146mg·mL^(-1)。GFE95的抗病毒作用较强,当样品质量浓度为200μg·mL^(-1)时,GFE95对EV71病毒的抑制率最高,达88.18%,其对EV71病毒抑制率的IC50值为194.80μg·mL^(-1),TI值为9.65。皮尔逊相关性分析结果表明DPPH、ABTS^(+)、O_(2)^(-)自由基清除率与三萜类成分含量呈极显著正相关,相关系数分别为0.992、0.971和0.613(P<0.01)。O_(2)^(-)、H_(2)O_(2)自由基清除率与多酚类成分含量呈极显著正相关,相关系数分别为0.921和0.997(P<0.01)。EV71病毒抑制作用与多酚、三萜类成分含量呈极显著正相关(P<0.01),相关系数分别为0.536和0.954。灰树花中三萜和多酚的含量与抗氧化、抗病毒活性具有较强的相关性。【结论】以体积分数为95%的乙醇溶液为提取剂,可有效提取灰树花中具有抗氧化、抗病毒活性的多酚、三萜、多糖等活性物质,提高灰树花的生物活性,是进一步促进灰树花的高值化应用开发的关键。研究结果为灰树花的提取溶剂选择及活性作用的研究奠定基础。

灰树花醇提物对α-葡萄糖苷酶和EV71的抑制作用及其活性成分分析

为了探明灰树花的降血糖与抗病毒作用,以及发挥活性作用的潜在活性物质,采用70%乙醇超声提取灰树花,依次用石油醚、乙酸乙酯、正丁醇3种不同极性溶剂对其提取物进行分步萃取,分别得到石油醚萃取物、乙酸乙酯萃取物、正丁醇萃取物和水溶物,测定其对α-葡萄糖苷酶和肠道病毒71型(EV71)的抑制作用.结果显示正丁醇萃取物对α-葡萄糖苷酶和EV71的抑制作用强于其他萃取物,其半抑制浓度分别为(174.12±10.67)、(84.41±5.12)μg·mL^(-1).利用超高效液相色谱质谱联用和分子对接技术发现,正丁醇萃取物中含量较高(11.58%)的亚油酸甲酯可通过与α-葡萄糖苷酶和EV71表面活性位点相结合而发挥活性作用.