Pear is an important fruit crop in the world. An uncharacterized disease has been observed on pear fruits during cold storage ir~ Suning, Shenzhou, Xinji and other locations in Hebei Province, China. The incidence rat...Pear is an important fruit crop in the world. An uncharacterized disease has been observed on pear fruits during cold storage ir~ Suning, Shenzhou, Xinji and other locations in Hebei Province, China. The incidence rate of the disease has reached 10%, and sometimes up to 20%. A particular fungus was consistently isolated from the infected pear fruit and cultured. Based on its morphology, molecular characteristics, pathogenicity and ITS sequence, the fungus was identified as Athelia bombacina. To our knowledge, this is the first report of Athelia bombacina causing postharvest fruit rot on pear.展开更多
Aims Ripe,fleshy fruits generally function as rewards to attract mutualistic seed dispersers,but many fruits also contain high concentrations of toxic secondary metabolites.These compounds may serve a variety of adapt...Aims Ripe,fleshy fruits generally function as rewards to attract mutualistic seed dispersers,but many fruits also contain high concentrations of toxic secondary metabolites.These compounds may serve a variety of adaptive roles in seed dispersal or as a defense against non-dispersing seed predators or pathogens.We tested the effects of iridoid glycosides from fruits of a hybrid bush honeysuckle,Lonicera×bella,on the growth of two pathogenic fungal strains associated with fruit rot,Alternaria tenuissima and Aspergillus tubingensis.Methods Fungi were isolated from field-collected L.×bella fruits and identified using molecular techniques.Their growth rates were assessed in vitro in the presence of varying concentrations of pure loganin,one of the most abundant iridoid glycosides in fruits,as well as fruit extracts containing a mix of at least seven different iridoid glycosides.Important FindingsLoganin had strong dose-dependent negative effects on the growth of both fungi.Extracts from fruits had no effect on Aspergillus but a strong antifungal effect on Alternaria that increased with fruit ripening.Total iridoid glycoside concentrations in extracts were not good predictors of variation in fungal growth,but several individual compounds had significant negative effects.Although iridoid glycosides have primarily been studied as antiherbivore defenses in leaves,these results indicate that they can also function to reduce the growth of fungi associated with fruit rot.展开更多
Fruit rot, besides causing losses in production, reduces the final quality of the product interfering in the commercialization. The objective of this work was to evaluate the efficiency of alternative products applied...Fruit rot, besides causing losses in production, reduces the final quality of the product interfering in the commercialization. The objective of this work was to evaluate the efficiency of alternative products applied to pre-harvest for control of brown rot (Monilinia fructicola) and soft rot (Rhizopus spp.) In peaches. The experiment was conducted in the municipality of Nepomuceno-MG, in a peach orchard of the cultivar Diamante. The experimental design was a randomized block design with 7 treatments and 3 replicates, with field plots formed by 12 plants (arranged in 3 rows), being considered for the evaluations only the two central plants. Three applications were made at flowering and at 21;7 and 3 days before harvest, with solutions containing the following treatments: 1-Clove oil 0.1%;2-silicate clay 1.5%;3-Phosphite K 0.20%;4-Benzalkonium chloride 0.25%;5-Azoxystrobin 0.02%;6-Iprodione 0.15%;7-Witness (water only). Treatments 4 and 5 were applied only at 21 and 7 days before harvest. Ten fruits were selected and placed in sterile trays under uncontrolled conditions, with disease evaluations at 3 and 6 days after the beginning of storage in 2005 and at 3;6 and 9 days in 2006. The iprodione controlled the incidence of M. fructicola and had no effect on Rhizopus spp. Clove oil controlled the incidence and severity of M. fructicola by the fifth day and Rhizopus spp. by the sixth day. The phosphites of k, benzalkonium chloride and azoxystrobin were efficient in controlling the incidence and severity of Rhizopus spp. and had no effect on M. fructicola.展开更多
A total of 123 Alternaria isolates were obtained from roting fruits of Pyrus bretschneideri "Ya Li" collected in Hebei Province, China. The isolates, according to morphological characteristics of conidia and...A total of 123 Alternaria isolates were obtained from roting fruits of Pyrus bretschneideri "Ya Li" collected in Hebei Province, China. The isolates, according to morphological characteristics of conidia and sporulation patterns, were segregated into four groups: A. alternata group, A. tenuissima group, A. yaliinficiens group and Alternaria sp. group. Molecular characteristics of part of the isolates were determined using random amplified polymorphism DNA (RAPD) analysis. Based on cluster analysis of RAPD data, large-and small-spored Alternaria spp. were evidently distinguished, and the small-spored species can form five clusters that fundamentally paralleled the morphological groupings.展开更多
Pear is a fruit crop of worldwide importance and cold storage is an integral part of the production and distribution of pears.An uncharacterized fungal disease has been observed on‘Huangguan’pear fruit during cold s...Pear is a fruit crop of worldwide importance and cold storage is an integral part of the production and distribution of pears.An uncharacterized fungal disease has been observed on‘Huangguan’pear fruit during cold storage in Hebei Province.The fungus was consistently isolated from diseased fruit by routine tissue separation method,and shown to be the causal agent according to Koch postulates.Based on its morphology,molecular characteristics,pathogenicity and ITS sequence,the fungus was identified as Rhizoctonia solani.This study recorded postharvest fruit rot caused by Rhizoctonia solani on pear fruit in China.展开更多
基金supported by a grant from the National Key R&D Program of China (2016YFD0400903-06)the earmarked fund for China Agriculture Research System (CARS-29-19)the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP)
文摘Pear is an important fruit crop in the world. An uncharacterized disease has been observed on pear fruits during cold storage ir~ Suning, Shenzhou, Xinji and other locations in Hebei Province, China. The incidence rate of the disease has reached 10%, and sometimes up to 20%. A particular fungus was consistently isolated from the infected pear fruit and cultured. Based on its morphology, molecular characteristics, pathogenicity and ITS sequence, the fungus was identified as Athelia bombacina. To our knowledge, this is the first report of Athelia bombacina causing postharvest fruit rot on pear.
基金University of Colorado Biological Sciences(Initiative BURST grant to J.T.,S.R.W.and M.D.B.)National Science Foundation(grant DEB 1210884 to S.R.W.and M.D.B.).
文摘Aims Ripe,fleshy fruits generally function as rewards to attract mutualistic seed dispersers,but many fruits also contain high concentrations of toxic secondary metabolites.These compounds may serve a variety of adaptive roles in seed dispersal or as a defense against non-dispersing seed predators or pathogens.We tested the effects of iridoid glycosides from fruits of a hybrid bush honeysuckle,Lonicera×bella,on the growth of two pathogenic fungal strains associated with fruit rot,Alternaria tenuissima and Aspergillus tubingensis.Methods Fungi were isolated from field-collected L.×bella fruits and identified using molecular techniques.Their growth rates were assessed in vitro in the presence of varying concentrations of pure loganin,one of the most abundant iridoid glycosides in fruits,as well as fruit extracts containing a mix of at least seven different iridoid glycosides.Important FindingsLoganin had strong dose-dependent negative effects on the growth of both fungi.Extracts from fruits had no effect on Aspergillus but a strong antifungal effect on Alternaria that increased with fruit ripening.Total iridoid glycoside concentrations in extracts were not good predictors of variation in fungal growth,but several individual compounds had significant negative effects.Although iridoid glycosides have primarily been studied as antiherbivore defenses in leaves,these results indicate that they can also function to reduce the growth of fungi associated with fruit rot.
文摘Fruit rot, besides causing losses in production, reduces the final quality of the product interfering in the commercialization. The objective of this work was to evaluate the efficiency of alternative products applied to pre-harvest for control of brown rot (Monilinia fructicola) and soft rot (Rhizopus spp.) In peaches. The experiment was conducted in the municipality of Nepomuceno-MG, in a peach orchard of the cultivar Diamante. The experimental design was a randomized block design with 7 treatments and 3 replicates, with field plots formed by 12 plants (arranged in 3 rows), being considered for the evaluations only the two central plants. Three applications were made at flowering and at 21;7 and 3 days before harvest, with solutions containing the following treatments: 1-Clove oil 0.1%;2-silicate clay 1.5%;3-Phosphite K 0.20%;4-Benzalkonium chloride 0.25%;5-Azoxystrobin 0.02%;6-Iprodione 0.15%;7-Witness (water only). Treatments 4 and 5 were applied only at 21 and 7 days before harvest. Ten fruits were selected and placed in sterile trays under uncontrolled conditions, with disease evaluations at 3 and 6 days after the beginning of storage in 2005 and at 3;6 and 9 days in 2006. The iprodione controlled the incidence of M. fructicola and had no effect on Rhizopus spp. Clove oil controlled the incidence and severity of M. fructicola by the fifth day and Rhizopus spp. by the sixth day. The phosphites of k, benzalkonium chloride and azoxystrobin were efficient in controlling the incidence and severity of Rhizopus spp. and had no effect on M. fructicola.
文摘A total of 123 Alternaria isolates were obtained from roting fruits of Pyrus bretschneideri "Ya Li" collected in Hebei Province, China. The isolates, according to morphological characteristics of conidia and sporulation patterns, were segregated into four groups: A. alternata group, A. tenuissima group, A. yaliinficiens group and Alternaria sp. group. Molecular characteristics of part of the isolates were determined using random amplified polymorphism DNA (RAPD) analysis. Based on cluster analysis of RAPD data, large-and small-spored Alternaria spp. were evidently distinguished, and the small-spored species can form five clusters that fundamentally paralleled the morphological groupings.
基金supported by the National Key R&D Program of China(Grant No.2016YFD0400903–06)the earmarked fund for China Agriculture Research System(Grant No.CARS-29–19)the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences。
文摘Pear is a fruit crop of worldwide importance and cold storage is an integral part of the production and distribution of pears.An uncharacterized fungal disease has been observed on‘Huangguan’pear fruit during cold storage in Hebei Province.The fungus was consistently isolated from diseased fruit by routine tissue separation method,and shown to be the causal agent according to Koch postulates.Based on its morphology,molecular characteristics,pathogenicity and ITS sequence,the fungus was identified as Rhizoctonia solani.This study recorded postharvest fruit rot caused by Rhizoctonia solani on pear fruit in China.
