Sensibility of male rats fertility against olive oil, Nigella sativa oil and pomegranate extract

Objective:To clarify the modulatory effects of daily consumption of pomegranate extract(PE),olive oil(OO)andNagilla sativaoil(NSO)on antioxidant activity,sperm quality and pituitary-testicular axis of adult male wistar rats.Methods:Thirty-two adult male Wistar rats were dividedinto four equal groups,eight rats each.Using rat gastric tubes,1.0 mL distilled water,1.0 mL PE,0.4mL NSO and 0.4 mL OO were orally administered daily for 6 weeks in the first,second,third andfourth groups,respectively.Reproductive organs,body weight,sperm criteria,testosterone,FSH,LH,inhibin-B,lipid peroxidation,and antioxidant enzyme activities were investigated.At the endof the study protocol,analyses occurred at the same time.Data were analysed by ANOVA test and P<0.05 was considered to be a significant value.Results:In all studied groups,malondialdehydelevel was significantly decreased accompanied with an increases in glutathione peroxidase andglutathione.Rats treated with PE showed an increase in catalase activities accompanied withan increase in sperm concentration which was also observed in NSO group.In PE treated group,sperm motility was also increased accompanied with decreased abnormal sperm rate.NSO,OOand PE treated groups shows an insignificant effect on testosterone,inhibin-B,FSH and LH incomparison with control group.Conclusions:These results show that administration of PE,NSOand OO could modify sperm characteristics and antioxidant activity of adult male wistar rats.

荆芥、防风挥发油抗炎作用的实验研究

对荆芥防风挥发油的抗炎作用进行试验 ,结果显示 :荆芥防风挥发油对二甲苯所致小鼠耳廓肿胀、小鼠腹腔毛细血管通透性、角叉菜胶致大鼠胸膜炎等急性炎症 ;对小鼠棉球肉芽种等慢性炎症和大鼠弗氏完全佐剂致关节炎肿胀、对小鼠耳异种被动皮肤过敏反应等过敏性炎症均有抑制作用 。

鱼油对KKAy糖尿病小鼠糖代谢及PI3K/Akt信号通路的影响

目的:探讨鱼油对KKAy糖尿病小鼠糖代谢及磷脂酰肌醇3-激酶(phosphatidylinositol 3-kinase,PI3K)/蛋白激酶B(protein kinase B,Akt)信号通路的影响。方法:40只KKAy小鼠,分为模型组和鱼油低、中、高剂量组,每组10只。另设10只C57BL/6小鼠为正常对照组。灌胃12周后,测定血糖、胰岛素、脂联素、瘦素及PI3K/Akt信号通路中相关基因的表达水平。结果:与模型组相比,鱼油低剂量组血糖水平极显著降低(P<0.01),鱼油低、中剂量组胰岛素水平显著提高(P<0.05),鱼油各剂量组的脂联素水平显著提高(P<0.05)。此外,鱼油各剂量组可上调胰岛素受体底物-1、PI3K、Akt、葡萄糖转运蛋白-4基因的表达,其中低剂量鱼油对Akt和葡萄糖转运蛋白-4基因的调控效果更显著。结论:低剂量鱼油可能通过调节PI3K/Akt信号通路改善糖代谢。

水酶法提取虹鳟鱼骨中鱼油工艺条件及优化

利用水酶法对虹鳟鱼加工副产物鱼头和鱼骨中的鱼油进行提取,并对酶解工艺条件进行优化。结果显示,中性蛋白酶作用鱼骨泥的提油效果优于动物蛋白酶、碱性蛋白酶和氨基肽酶;鱼骨泥加等质量水混匀后,添加0.4%中性蛋白酶在自然p H、40℃下酶解3.5 h,提油效果最佳,提油率达90.55%。利用水酶法提取的鱼油酸价为2.25~4.25mg/g,碘值为138~139 g/100 g,皂化值为492.06~501.39 mg/g,茴香胺值为9.1~22.9;鱼油中饱和脂肪酸含量21.78 g/100g,单不饱和脂肪酸含量30.80 mg/100 g,多不饱和脂肪酸含量48.43 g/100 g,EPA含量1.07 g/100 g,DHA含量3.61 g/100g。利用水酶法提取虹鳟鱼骨中鱼油提油效果较好,鱼油品质较高,可进行资源开发与利用。