The ovary is an excellent system for studying stem cell renewal and differentiation, which is under the control of ovarian somatic cells. In order to understand oogenesis in Fugu rubripes （Temminck et. Schlegel） as ...The ovary is an excellent system for studying stem cell renewal and differentiation, which is under the control of ovarian somatic cells. In order to understand oogenesis in Fugu rubripes （Temminck et. Schlegel） as a marine fish model of aquaculture importance, we established cell lines called TSOC 1 and TSOC2 from a juvenile ovary of this organism. TSOC1 is composed of spindle epithelial-like cells, while the other is cobblestone-like ceils. Therefore, TSOC1 and TSOC2 appear to consist of ovarian somatic cells. Growth requirement condition was investigated including temperature, concentration of FBS and pH. Significant fluorescent signals were observed after TSOC1 and TSOC2 cells were transfected with pEGFP-N3 vector, indicating its potential utility for genetic manipulation such as gene function studies. It is shown that these cell lines are effective for infection by the turbot reddish body iridovirus and flounder lymphosystis disease virus as evidenced by the appearance of cytopathic effect and virus propagation in the virus-infected cells, and most convincingly, the observation of viral particles by electron microscopy, demonstrating that TSOC1 and TSOC2 are suitable to study interactions between virus and host cells. It is believed that TSOC1 and TSOC2 will be useful tools to study sex-related events and interactions between primordial germ cells and oogonia cells during oogenesis. Therefore, establishment of ovary cell lines from Fugu rubripes seems to be significant for those research areas.展开更多
Based on the Leaderman constitutive relations in nonlinear viscoelasticity and the linear geometrical assumption, a mathematical model for the bending of nonlinear viscoelastic beams was established in this paper. The...Based on the Leaderman constitutive relations in nonlinear viscoelasticity and the linear geometrical assumption, a mathematical model for the bending of nonlinear viscoelastic beams was established in this paper. The Laplace transformation method and the Titchmarsh theorem were used to prove that some relations exist between solutions to bending problems of visco- and elastic beams, which reveals the fugue effect of viscoelastic materials. The high-order Galerkin approximate solution to the quasi-static response of nonlinear viscoelastic beams under a step load was obtained by using the new method suggested in this paper as well as the Mathematica software and the Newton iteration technique.展开更多
Carson McCullers The Heart is a Lonely Hunter is her representative work. McCullers deploys the skill of fugue pattern through the multi-voices reflected in the characters, tactfully unite the form and content into a ...Carson McCullers The Heart is a Lonely Hunter is her representative work. McCullers deploys the skill of fugue pattern through the multi-voices reflected in the characters, tactfully unite the form and content into a whole. By adopting Bakhtin's theory of polyphony, this paper aims to analyze the fugue pattern together with its multi-voices in the three parts, namely, the exposition, the development and the return reflected in the novel, which highlights its theme of universal loneliness so impressively.展开更多
In this study, genes of two distinct tissue inhibitors of metalloproteinases-2 (TIMP-2) from Japanese puffer fishFugu rubripes, Fugu TIMP-2a and TIMP-2b, were cloned. The open reading frames of Fugu TIMP-2a and TIMP-2...In this study, genes of two distinct tissue inhibitors of metalloproteinases-2 (TIMP-2) from Japanese puffer fishFugu rubripes, Fugu TIMP-2a and TIMP-2b, were cloned. The open reading frames of Fugu TIMP-2a and TIMP-2b cDNAsare composed of 660 and 657 nucleotides and 220 and 219 amino acids, respectively. Both Fugu TIMP-2s contain 12 cysteineresidues, which might form six disulfide bonds as in other animals’ TIMP-2s. Reverse-transcribed polymerase chain reactionanalysis showed the mRNAs of Fugu TIMP-2a and TIMP-2b to be expressed in some tissues examined with different expres-sion patterns. These findings suggest that the two distinct Fugu TIMP-2s might perform different functions in Fugu tissues.展开更多
基金The finance special program of Tianjinthe transformation project of Tianjin Agricultural Achievements
文摘The ovary is an excellent system for studying stem cell renewal and differentiation, which is under the control of ovarian somatic cells. In order to understand oogenesis in Fugu rubripes （Temminck et. Schlegel） as a marine fish model of aquaculture importance, we established cell lines called TSOC 1 and TSOC2 from a juvenile ovary of this organism. TSOC1 is composed of spindle epithelial-like cells, while the other is cobblestone-like ceils. Therefore, TSOC1 and TSOC2 appear to consist of ovarian somatic cells. Growth requirement condition was investigated including temperature, concentration of FBS and pH. Significant fluorescent signals were observed after TSOC1 and TSOC2 cells were transfected with pEGFP-N3 vector, indicating its potential utility for genetic manipulation such as gene function studies. It is shown that these cell lines are effective for infection by the turbot reddish body iridovirus and flounder lymphosystis disease virus as evidenced by the appearance of cytopathic effect and virus propagation in the virus-infected cells, and most convincingly, the observation of viral particles by electron microscopy, demonstrating that TSOC1 and TSOC2 are suitable to study interactions between virus and host cells. It is believed that TSOC1 and TSOC2 will be useful tools to study sex-related events and interactions between primordial germ cells and oogonia cells during oogenesis. Therefore, establishment of ovary cell lines from Fugu rubripes seems to be significant for those research areas.
文摘Based on the Leaderman constitutive relations in nonlinear viscoelasticity and the linear geometrical assumption, a mathematical model for the bending of nonlinear viscoelastic beams was established in this paper. The Laplace transformation method and the Titchmarsh theorem were used to prove that some relations exist between solutions to bending problems of visco- and elastic beams, which reveals the fugue effect of viscoelastic materials. The high-order Galerkin approximate solution to the quasi-static response of nonlinear viscoelastic beams under a step load was obtained by using the new method suggested in this paper as well as the Mathematica software and the Newton iteration technique.
文摘Carson McCullers The Heart is a Lonely Hunter is her representative work. McCullers deploys the skill of fugue pattern through the multi-voices reflected in the characters, tactfully unite the form and content into a whole. By adopting Bakhtin's theory of polyphony, this paper aims to analyze the fugue pattern together with its multi-voices in the three parts, namely, the exposition, the development and the return reflected in the novel, which highlights its theme of universal loneliness so impressively.
基金partially supported by a grant from the Ministry of Education,Culture,Sports,Science and Technology of Japan.
文摘In this study, genes of two distinct tissue inhibitors of metalloproteinases-2 (TIMP-2) from Japanese puffer fishFugu rubripes, Fugu TIMP-2a and TIMP-2b, were cloned. The open reading frames of Fugu TIMP-2a and TIMP-2b cDNAsare composed of 660 and 657 nucleotides and 220 and 219 amino acids, respectively. Both Fugu TIMP-2s contain 12 cysteineresidues, which might form six disulfide bonds as in other animals’ TIMP-2s. Reverse-transcribed polymerase chain reactionanalysis showed the mRNAs of Fugu TIMP-2a and TIMP-2b to be expressed in some tissues examined with different expres-sion patterns. These findings suggest that the two distinct Fugu TIMP-2s might perform different functions in Fugu tissues.
