Photodissociation Dynamics of 2-1odotoluene Investigated by Femtosecond Time-Resolved Mass Spectrometry

The photodissociation dynamics of 2-iodotoluene following excitation at 266 nm have been investigated employing femtosecond time-resolved mass spectrometry. The photofragments are detected by multiphoton ionization using an intense laser field centered at 800 nm. A dissociation time of 3804-50 fs was measured from the rising time of the co-fragments of toluene radical （C7H7） and iodine atom （I）, which is attributed to the averaged time needed for the C-I bond breaking for the simultaneously excited nσ and ππ＊ states by 266 nm pump light. In addition, a probe light centered at 298.23 nm corresponding to resonance wavelength of ground-state iodine atom is used to selectively ionize ground-state iodine atoms generated from the dissociation of initially populated hσ＊ and ππ＊ states. And a rise time of 4004-50 fs is extracted from the fitting of time-dependent I＋ transient, which is in agreement with the dissociation time obtained by multiphoton ionization with 800 nm, suggesting that the main dissociative products are ground-state iodine atoms.

Impact of time lags on diurnal estimates of canopy transpiration and canopy conductance from sap-flow measurements of Populus cathayana in the Qinghai–Tibetan Plateau

Recently, canopy transpiration （Ec） has been often estimated by xylem sap-flow measurements. However, there is a significant time lag between sap flow measured at the base of the stem and canopy transpiration due to the capacitive exchange between the transpiration stream and stem water storage. Significant errors will be introduced in canopy conductance （gc） and canopy transpiration estimation if the time lag is neglected. In this study, a cross-correlation analysis was used to quantify the time lag, and the sap flowbased transpiration was measured to pararneterize Jarvistype models of gc and thus to simulate Ec of Populus cathayana using the Penman-Monteith equation. The results indicate that solar radiation （Rs） and vapor pressure deficit （VPD） are not fully coincident with sap flow and have an obvious lag effect; the sap flow lags behind Rs and precedes VPD, and there is a 1-h time shift between Eo and sap flow in the 30-min interval data set. A parameterized Jarvis-type gc model is suitable to predict P. cathayana transpiration and explains more than 80% of the variation observed in go, and the relative error was less than 25%, which shows a preferable simulation effect. The root mean square error （RMSEs） between the predicted and measured Ec were 1.91×10^-3 （with the time lag） and 3.12×10^-3cm h^-1 （without the time lag）. More importantly, Ec simulation precision that incorporates time lag is improved by 6% compared to the results without the time lag, with the mean relative error （MRE） of only 8.32% and the mean absolute error （MAE） of 1.48 × 10^-3 cm h^-1.

Real-time crash prediction on freeways using data mining and emerging techniques

Recent advances in intelligent transportation system allow traffic safety studies to extend from historic data-based analyses to real-time applications. The study presents a new method to predict crash likelihood with traffic data collected by discrete loop detectors as well as the web-crawl weather data. Matched case-control method and support vector machines （SVMs） technique were employed to identify the risk status. The adaptive synthetic over-sampling technique was applied to solve the imbalanced dataset issues. Random forest technique was applied to select the contributing factors and avoid the over-fitting issues. The results indicate that the SVMs classifier could successfully classify 76.32% of the crashes on the test dataset and 87.52% of the crashes on the overall dataset, which were relatively satisfactory compared with the results of the previous studies. Compared with the SVMs classifier without the data, the SVMs classifier with the web-crawl weather data increased the crash prediction accuracy by 1.32% and decreased the false alarm rate by 1.72%, showing the potential value of the massive web weather data. Mean impact value method was employed to evaluate the variable effects, and the results are identical with the results of most of previous studies. The emerging technique based on the discrete traffic data and web weather data proves to be more applicable on real- time safety management on freeways.

A Numerical Algorithm Based on Quadratic Finite Element for Two-Dimensional Nonlinear Time Fractional Thermal Diffusion Model

In this article,a high-order scheme,which is formulated by combining the quadratic finite element method in space with a second-order time discrete scheme,is developed for looking for the numerical solution of a two-dimensional nonlinear time fractional thermal diffusion model.The time Caputo fractional derivative is approximated by using the L2-1formula,the first-order derivative and nonlinear term are discretized by some second-order approximation formulas,and the quadratic finite element is used to approximate the spatial direction.The error accuracy O(h3+
t2)is obtained,which is verified by the numerical results.

Suppressive Influence of Time- Space White Noise on the Explosion of Solutions of Stochastic Fokker- Planck Delay Differential Equations

It is generally known that the solutions of deterministic and stochastic differential equations （SDEs） usually grow linearly at such a rate that they may become unbounded after a small lapse of time and may eventually blow up or explode in finite time. If the drift and diffusion functions are globally Lipschitz, linear growth may still be experienced, as well as a possible blow-up of solutions in finite time. In this paper, a nonlinear scalar delay differential equation with a constant time lag is perturbed by a multiplicative Ito-type time - space white noise to form a stochastic Fokker-Planck delay differential equation. It is established that no explosion is possible in the presence of any intrinsically slow time - space white noise of Ito - type as manifested in the resulting stochastic Fokker- Planck delay differential equation. Time - space white noise has a role to play since the solution of the classical nonlinear equation without it still exhibits explosion.

人5-羟色胺2c受体基因实时荧光定量PCR方法的建立

目的建立5-羟色胺2c受体（5-HTR2c）基因mRNA表达水平的TaqMan real—time PcR检测方法。方法以β-actin为内参基因，根据GenBank中人5-HTR2c及β-actin基因序列，分别设计了2套特异性引物和TaqMan探针，接着对反应的退火温度、引物浓度、探针浓度、Mg^2＋浓度进行优化，然后以优化的条件建立相对定量标准曲线，并对该方法的稳定性进行了分析。结果5-HTR2c及β-actin基因的real—time PCR扩增效率分别为99.9％和100.0％；相对定量标准曲线的CT值线性范围分别为12．2～35．1和11．5～31．5，相关系数分别为0．999及1．000；批内及批问变异系数（8．0％。结论本研究所建立的针对5-HTR2c mRNA表达水平的Taqman real-time PCR检测方法具有扩增效率高、稳定性好等特点，为进一步探索5-HTR2c的功能及其mRNA表达水平的变化和各种疾病发生、发展的相关性提供了有用的方法学基础。

基于交流注入法的大容量铅碳电池内阻测量

电池内阻是判断电池健康程度和放电能力的重要参数,而目前针对大容量铅碳电池内阻检测方面的相关研究较少,因此提出一种大容量铅碳电池的交流注入检测法。首先对铅碳电池等效电路模型进行推导分析;其次应用Simulink软件建立检测系统仿真模型,验证其电阻检测过程,仿真结果与被测铅碳电池内阻一致,说明该检测系统方案可行;最后采用新威高性能电池检测系统作为标准检测仪器进行对比测试验证,达到实际应用要求。该测试方法对大容量铅碳电池内阻的检测具有一定的借鉴作用。

MALDI-TOF-MS法对重组人内皮抑素蛋白分子质量测定

肿瘤的生长依赖于血管的生成,新生血管不仅为肿瘤生长提供必需的营养物质,而且为肿瘤细胞扩散提供了重要的途径[1].1997年哈佛大学的O'Reilly等[2]发现了一种内源性新血管生成抑制因子内皮抑素(Endostatin),显示出特异抑制激活的血管内皮细胞增殖和肿瘤新血管生成的生物学活性,其抗肿瘤作用具有高效、低毒、无耐药性的优点.……

小麦醇溶-谷蛋白盒结合因子基因表达特性的研究

为了给小麦品质改良的基因工程研究提供参考依据,以小麦醇溶-谷蛋白盒结合因子(Wheat Prola-min-Box Binding Factor,WPBF)基因为研究对象,从定性和定量两个方面,利用RT-PCR与Real Time PCR系统地研究了WPBF基因在小麦不同组织器官中以及在小麦胚乳整个发育过程中表达的时空特异性。结果表明,WPBF基因的表达是胚乳特异性的,表达跨胚乳的整个发育过程,在其表达的高峰阶段,表达强度为参照基因β-Actin的43%。

气象观测场在汽车试验场中的应用研究

汽车试验场作为汽车开展道路测试的重要场所,用于验证汽车产品的品质以及可靠性。除了场地道路外,气象条件作为汽车道路测试的重要一环,在《GB/T12534-1990汽车道路试验方法通则》中也有明确要求,如:试验时应是无雨无雾天气,相对湿度小于95%,气温0-40℃,风速不大于3m/s。同时气象条件也作为试验场道路管控的重要依据,实时风速、雨量、能见度等信息为场地管理者发布限速、限行、封场等通知提供必要参考依据,直接影响道路测试安全管控的及时性。因此,文章从气象观测场的建设、气象服务、异常天气道路管控等方面开展气象观测场在汽车试验场中的应用研究。

Stratigraphical Time——Correlation and Mass Extinction Event Near Permian——Triassic Boundary in South China

Shaw's method used to correlate 40 sections across the Permo-Triassic boundary in South China is applied in the paper. Two steps are adopted to get an Integral Composite Section (ICS) by synthesizing these data : First , South China is divided into five areas and composite section developed for each area . Then the second step . the Changxing composite section is regarded as a composite standard (CSRS) while the ICS is produced by matching the CSRS with composite sections of the other areas. Three biozones in the Changxingian and two biozones in the Griesbachian can be discerned on the basis of computing Z values in the ICS. These biozones are marked by the Z values which quantitatively represent their time ranges ; therefore , they may increase accuracy of stratigraphic time correlation . The mass extinction at the end of the Permian is an abrupt event that is supported by the relative rate of extinction near the P/T boundary . About 90% of invertebrate species died out by the end of the Permian . The duration of the mass extinction is rather short ,approximately 0.018Ma .

Characterizing long-term forest disturbance history and its drivers in the Ning-Zhen Mountains, Jiangsu Province of eastern China using yearly Landsat observations (1987–2011)

Forest losses or gains have long been recognized as critical processes modulating the carbon flux between the biosphere and the atmosphere. Timely, accurate and spatially explicit information on forest disturbance and recovery history is required for assessing the effectiveness of existing forest management. The major objectives of our research focused on testing the mapping efficacy of the vegetation change tracker （VCT） model over a forested area in China. We used a new version of VCT algorithm built upon the Landsat time series stacks （LTSS）. The LTSS consisted of yearly image acquisitions to map forest disturbance history from 1987 to 2011 over the Ning-Zhen Mountains, Jiangsu Province of east China. The LTSS consisted of TM and ETM＋ scenes with different projec- tions due to distinct data sources （Beijing remote sensing ground station and the USGS EROS Center）. The valida- tion results of the disturbance year maps showed that most spatial agreement measures ranged from 70 to 86 %, comparable with the VCT accuracies reported for many places in USA. Very low accuracies were identified in 1995 （38.3 %） and 1992 （56.2 %） in the current analysis. These resulted from the insensitivity of the VCT algorithm to detect low intensity disturbances and also from the mis- registration errors of the image pairs. Major forest distur- bance types existing in our study area were identified as agricultural expansion （39.8 %）, urbanization （24.9 %）, forest management practice （19.3 %）, and mining （12.8 %）. In general, there was a gradual decreasing trend in forest cover throughout this region, caused principally by China＇s economic, demographic, environmental and political policies and decisions, as well as some weather events. While VCT has largely been used to assess long term changes and trends in the USA, it has great potential for assessing landscape level change elsewhere throughout the world.

2-(2-Benzofuranyl)-2-imidazoline treatment within 5 hours after cerebral ischemia/reperfusion protects the brain

We previously demonstrated that administering 2-（2-benzofuranyl）-2-imidazolin（2-BFI）, an imidazoline I2 receptor agonist, immediately after ischemia onset can protect the brain from ischemic insult. However, immediate administration after stroke is difficult to realize in the clinic. Thus, the therapeutic time window of 2-BFI should be determined. Sprague-Dawley rats provided by Wenzhou Medical University in China received right middle cerebral artery occlusion for 120 minutes, and were treated with 2-BFI（3 mg/kg） through the caudal vein at 0, 1, 3, 5, 7, and 9 hours after reperfusion. Neurological function was assessed using the Longa＇s method. Infarct volume was measured by 2,3,5-triphenyltetrazolium chloride assay. Morphological changes in the cortical penumbra were observed by hematoxylin-eosin staining under transmission electron microscopy. The apoptosis levels in the ipsilateral cortex were examined with terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling（TUNEL） assay. The protein expression of Bcl-2 and BAX was detected using immunohistochemistry. We found the following： Treatment with 2-BFI within 5 hours after reperfusion obviously improved neurological function. Administering 2-BFI within 9 hours after ischemia/reperfusion decreased infarct volume and alleviated apoptosis. 2-BFI administration at different time points after reperfusion alleviated the pathological damage of the ischemic penumbra and reduced the number of apoptotic neurons, but the protective effect was more obvious when administered within 5 hours. Administration of 2-BFI within 5 hours after reperfusion remarkably increased Bcl-2 expression and decreased BAX expression. To conclude, 2-BFI shows potent neuroprotective effects when administered within 5 hours after reperfusion, seemingly by up-regulating Bcl-2 and down-regulating BAX expression. The time window provided clinical potential for ischemic stroke by 2-BFI.

Decentralized Stabilization of Large-scale Uncertain Systems with State-Delays——LMIs Approach

This paper deals with the problem of decentralized robustcontrol for a class of interconnected uncertain systemswith state delays.The parameter uncertainties are un-known but norm-bounded.A new sufficient condition isobtained for each subsystem and overall system to be sta-bilizable via linear memoryless state feedback robust de-centralized controllers.The results depend on the size of the delays and are given in terms of linear matrix ine-qualities,so they are less conservative than those of delay-independent.Moreover,matching condition is not a necessary condition.Finally,an example is presented to illustrative the effectiveness of the proposed method.

Non-stationary Buffeting Response Analysis of Long Span Suspension Bridge Under Strong Wind Loading

The non-stationary buffeting response of long span suspension bridge in time domain under strong wind loading is computed. Modeling method for generating non-stationary fluctuating winds with probabilistic model for non-stationary strong wind fields is first presented. Non-stationary wind forces induced by strong winds on bridge deck and tower are then given a brief introduction. Finally,Non-stationary buffeting response of Pulite Bridge in China,a long span suspension bridge,is computed by using ANSYS software under four working conditions with different combination of time-varying mean wind and time-varying variance. The case study further confirms that it is necessity of considering non-stationary buffeting response for long span suspension bridge under strong wind loading,rather than only stationary buffeting response.

Creative Design Fulfills Brand and Fashion China Fashion Week A/W 2010-2011 Collection held in Beijing

March is a right party time for friends to get together to enjoy the smell of early spring. Moreover, people would like to take off the thick winter coat to showcase their colorful attitude towards the warm spring sunshine. Here, an annual fashion feast in Beijing not only could provide you a visual inspiration of trend, but also would tell you what’s the most "in" factors in the coming season.

高校一线专职辅导员队伍建设的现状、困境与出路

通过对广州某市属高校一线专职辅导员队伍建设现状的调研显示,辅导员队伍建设制度完善、队伍结构相对合理,但是人员配备有待加强、行政职务难以提升,专业化、职业化建设程度不高等,需要政府落实政策、学校提供平台、辅导员做好工作三方共同努力,促进辅导员队伍朝专业化、职业化发展。

高校专职辅导员+兼职班主任队伍模式探析

专职辅导员加班主任的学生工作模式,将辅导员从繁重的日常事务管理中解脱出来;专业教师担当班主任工作也避免了过去辅导员专业知识的不足,为高校学生的学业指导提供了保障。

高校专职辅导员团队建设的实践与探索

加强高校专职辅导员团队建设对促进学生健康成长和维护校园安全稳定等方面,具有十分重要的意义。针对目前高校专职辅导员团队建设现状,笔者从团队目标、成员素质、团队结构和完善机制等几方面提出高校专职辅导员团队建设的实现途径。

GM计数管量程扩展技术进展

GM计数管是一种应用广泛的核辐射探测器,但量程范围较窄,扩展其量程能进一步拓宽其应用范围。回顾了国内外扩展GM计数管量程所采取的主要方法和措施,分析了采用死时间模型和函数拟合修正方法扩展量程存在的不足;简要介绍和分析了改变计数管工件方式或改进外部电路的量程扩展方法,重点介绍了近年来出现的time-to-count方法的工作原理。指出通过革新使用方式,改进计数管结构设计和筛选恰当材料,研制复合型探测器等途径,可大幅提高计数管的性能。