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Inhibition of M-MuLV reverse transcriptase activity by fullerene derivatives 被引量:4
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作者 MENG Xianmei CHEN Zhe LI Bo ZHANG Yufei ZHAO Dongxu YANG Xinlin 《Chinese Science Bulletin》 SCIE EI CAS 2006年第20期2550-2552,共3页
Influence of fullerene (C60) derivatives on M-MuLV reverse transcriptase activity is investi- gated. Two water-soluble fullerene derivatives, fullerol (C60(OH)23-24) and trimalonic acid C60 (TMA C60, C63(COOH)6) are u... Influence of fullerene (C60) derivatives on M-MuLV reverse transcriptase activity is investi- gated. Two water-soluble fullerene derivatives, fullerol (C60(OH)23-24) and trimalonic acid C60 (TMA C60, C63(COOH)6) are used in the experiments and their effects on in vitro reverse transcription-polymerase chain reaction (RT-PCR) of β-actin mRNA in HeLa cells are determined. PCR products are detected by agarose gel electrophoresis. It is found that the amounts of PCR products decrease with addition of either of two fullerene derivatives to RT reaction mixture. The inhibition of fullerene derivatives is dose-dependent, and IC50 values of fullerol and TMA C60 are 0.089 and 0.039 mmol/L, respectively. The amount of PCR products obtained by direct addition of fullerol or TMA C60 to PCR are greater than those obtained by addition of the equivalent amount of fullerol or TMA C60 to RT, indicating an inhibitory ef- fect of fullerol or TMA C60 on RT. The compensative experiment of M-MuLV reverse transcriptase shows that increasing enzyme amounts can antagonize the activity of fullerol or TMA C60. These results imply that fullerenes can inhibit M-MuLV reverse transcriptase activity, with the inhibition of TMA C60 slightly stronger than fullerol, and that their potential in treatment of diseases induced by RNA viruses such as leukemia virus needs further investigation. 展开更多
关键词 m-mulv 反转录酶 抑制作用 水溶性 丙二酸
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