Synthesis of ZSM-5 zeolite typically utilizes small molecule polyamines or quaternary ammonium salts as organic structure guiding agent(OSDA).By contrast,the OSDA-free hydrothermal synthesis system eliminates the use ...Synthesis of ZSM-5 zeolite typically utilizes small molecule polyamines or quaternary ammonium salts as organic structure guiding agent(OSDA).By contrast,the OSDA-free hydrothermal synthesis system eliminates the use of organic templates and the subsequent calcination procedure.This not only reduces the cost of synthesis,but also prevents environmental pollution from the combustion of organic templates,representing an eco-friendly approach.Despite this,literature suggests that even so-called template-free synthesis systems often involve trace amount of organic substances like alcohol.In the present work,a calcined commercial ZSM-5 zeolite was served as seed,with sodium aluminate as aluminum source and silica sol as silicon source,ensuring an entirely template-free synthesis system.Polycrystalline ZSM-5 aggregates consisted of rod-like nanocrystals were successfully prepared in the completely OSDA-free system.Effects of the Si/Al ratio in ZSM-5 seed,dosage and crystallization conditions such as crystallization temperature and crystallization time on ZSM-5 synthesis were investigated.The results show that a highly crystallinity ZSM-5 aggregate consisting of primary nano-sized crystals less than 100 nm is produced from a gel precursor with 5.6%(in mass)seed after hydrothermal treatment for 48 h.Furthermore,the Si/Al ratio in ZSM-5 seed has little effect on the topological structure and pore structure of the synthesized samples.However,the seeds with a low Si/Al ratio facilitate faster crystallization of zeolite and enhance the acidity,especially the strong acid centers,of the catalyst.The catalytic performance of the synthesized polycrystalline ZSM-5 was evaluated during dehydration of methanol and compared with a commercial reference ZSM-5r.The results exhibit that as compared with the reference catalyst,the fabricated sample has a longer catalytic lifetime(16 h vs 8 h)attributed to its hierarchical pores derived from the loosely packed primary nanoparticles.Additionally,the prepared polycrystalline catalyst also exhibits a higher aromatics selectivity(28.1%-29.8%vs 26.5%).展开更多
Seeding is an important part of improving corn yield.Currently,seed guide tubes are mostly used as transport devices.But the existing seed guide tubes cannot meet the requirements or achieve the goal of fixing the see...Seeding is an important part of improving corn yield.Currently,seed guide tubes are mostly used as transport devices.But the existing seed guide tubes cannot meet the requirements or achieve the goal of fixing the seed falling trajectory.A seed collision phenomenon occurs occasionally.So,in response to the problems that the seeds and seed guide tube collide or bounce under high speed operation,which results in a lower sowing qualification rate and poor spacing uniformity,a seed receiving and conveying system comprising a belt-type high-speed corn seed guiding device was designed and optimized,to meet the needs of high-speed precision sowing operations and improve the spacing uniformity.The factors affecting the seed conveying performance were obtained by analyzing the mechanical properties of the seeds at various movement stages.These factors were the number of seed cavities between adjacent seeds,the forward speed,the height from the ground,and the installation angle.Single factor simulation experiments were conducted by selecting the paddle spacing as the test factor and using the pass rate,reseeding rate,omission rate and coefficient of variation as the evaluation indexes to investigate the influence of the paddle spacing on the seed guide performance of the device and further determine the structural parameters of the paddle belt.Orthogonal rotation combination tests of three factors and five levels were also conducted through bench testing.Then the test outcomes were optimized.The results indicated that the best results were obtained when the number of seed cavity intervals between adjacent seeds was 5.16,the installation angle was 79.40°,and the height from the ground was 31.84 mm.At this time,the qualified rate was 98.49%,the repeated sowing rate was 0.48%,the missed sowing rate was 1.03%,and the coefficient of variation was 6.80%.Experiments were used to validate the optimization results,and all of the obtained index data satisfied the criteria for accurate and quick corn sowing.The study’s findings can serve as a theoretical foundation for a belt-type high-speed corn seed guiding device optimization test.展开更多
It was hypothesized that mesenchymal stem cells(MSCs) could provide necessary trophic factors when seeded onto the surfaces of commonly used nerve graft substitutes. We aimed to determine the gene expression of MSCs w...It was hypothesized that mesenchymal stem cells(MSCs) could provide necessary trophic factors when seeded onto the surfaces of commonly used nerve graft substitutes. We aimed to determine the gene expression of MSCs when influenced by Avance■ Nerve Grafts or Neura Gen■ Nerve Guides. Human adipose-derived MSCs were cultured and dynamically seeded onto 30 Avance■ Nerve Grafts and 30 Neura Gen■ Nerve Guides for 12 hours. At six time points after seeding, quantitative polymerase chain reaction analyses were performed for five samples per group. Neurotrophic [nerve growth factor(NGF), glial cell line-derived neurotrophic factor(GDNF), pleiotrophin(PTN), growth associated protein 43(GAP43) and brain-derived neurotrophic factor(BDNF)], myelination [peripheral myelin protein 22(PMP22) and myelin protein zero(MPZ)], angiogenic [platelet endothelial cell adhesion molecule 1(PECAM1/CD31) and vascular endothelial cell growth factor alpha(VEGFA)], extracellular matrix(ECM) [collagen type alpha I(COL1A1), collagen type alpha III(COL3A1), Fibulin 1(FBLN1) and laminin subunit beta 2(LAMB2)] and cell surface marker cluster of differentiation 96(CD96) gene expression was quantified. Unseeded Avance■ Nerve Grafts and Neura Gen■ Nerve Guides were used to evaluate the baseline gene expression, and unseeded MSCs provided the baseline gene expression of MSCs. The interaction of MSCs with the Avance■ Nerve Grafts led to a short-term upregulation of neurotrophic(NGF, GDNF and BDNF), myelination(PMP22 and MPZ) and angiogenic genes(CD31 and VEGFA) and a long-term upregulation of BDNF, VEGFA and COL1A1. The interaction between MSCs and the Neura Gen■ Nerve Guide led to short term upregulation of neurotrophic(NGF, GDNF and BDNF) myelination(PMP22 and MPZ), angiogenic(CD31 and VEGFA), ECM(COL1A1) and cell surface(CD96) genes and long-term upregulation of neurotrophic(GDNF and BDNF), angiogenic(CD31 and VEGFA), ECM genes(COL1A1, COL3A1, and FBLN1) and cell surface(CD96) genes. Analysis demonstrated MSCs seeded onto Neura Gen■ Nerve Guides expressed significantly higher levels of neurotrophic(PTN), angiogenic(VEGFA) and ECM(COL3A1, FBLN1) genes in the long term period compared to MSCs seeded onto Avance■ Nerve Grafts. Overall, the interaction between human MSCs and both nerve graft substitutes resulted in a significant upregulation of the expression of numerous genes important for nerve regeneration over time. The in vitro interaction of MSCs with the Neura Gen■ Nerve Guide was more pronounced, particularly in the long term period(> 14 days after seeding). These results suggest that MSC-seeding has potential to be applied in a clinical setting, which needs to be confirmed in future in vitro and in vivo research.展开更多
Endoscopic ultrasonography-guided fine needle aspiration(EUS-FNA) is a useful and relatively safe tool for the diagnosis and staging of pancreatic cancer. However, there have recently been several reports of tumor see...Endoscopic ultrasonography-guided fine needle aspiration(EUS-FNA) is a useful and relatively safe tool for the diagnosis and staging of pancreatic cancer. However, there have recently been several reports of tumor seeding after EUS-FNA of adenocarcinomas. A 78-year-old man was admitted to our hospital due to upper gastric pain. Examinations revealed a 20 mm mass in the pancreatic body, for which EUS-FNA was performed. The cytology of the lesion was adenocarcinoma, and the stage of the cancer was T3N0M0. The patient underwent surgery with curative intent, followed by adjuvant chemotherapy with S-1. An enlarging gastric submucosal tumor was found on gastroscopy at 28 mo after surgery accompanied by a rising level of CA19-9. Biopsy result was adenocarcinoma, consistent with a pancreatic primary tumor. Tumor seeding after EUS-FNA was strongly suspected. The patient underwent surgical resection of the gastric tumor with curative intent. The pathological result of the resected gastric specimen was adenocarcinoma with a perfectly matched mucin special stain result with the previously resected pancreatic cancer. This is the first case report of tumor seeding after EUS-FNA which was surgically resected and inspected pathologically.展开更多
We reported two cases of liver metastasis with portal vein tumor thrombus that developed after liver transplantation for hepato cellular carcinoma (HCC). Both the patients were women aged 43 and 55 years, who had live...We reported two cases of liver metastasis with portal vein tumor thrombus that developed after liver transplantation for hepato cellular carcinoma (HCC). Both the patients were women aged 43 and 55 years, who had liver metastasis and portal vein tumor thrombus formation after liver transplantations for HCC.展开更多
To meet the requirement of“zero speed”for precision sowing,the instantaneous speed of seed falling into the seed bed should be equal to the opposite direction of the forward speed of the machine.Through analyzing th...To meet the requirement of“zero speed”for precision sowing,the instantaneous speed of seed falling into the seed bed should be equal to the opposite direction of the forward speed of the machine.Through analyzing the principle of seed guiding process,the main influencing factors affecting the contour curve of the seed tube were determined to plan the seed guiding path and realize zero speed seed delivery.With the help of EDEM simulation software,taking the inclination angle of seed tube end,operating speed of the metering disc,and machine’s forward speed as the experimental factors,a single-factor simulation test was conducted with the pass rate,replay rate,and missing seeding rate as experimental indexes.The results of the simulation test showed that the range of inclination angle of the seed tube end was 23°-40°,the range of machine forward speed was 5-9 km/h,and the range of operating speed of the metering disc was 15-40 r/min.The quadratic orthogonal rotation combination test method was carried out using the bench test,and a mathematical regression model was established between experimental factors and experimental indexes to determine the priority order of factors,and the best combination of factors.The test results showed that the pass rate was 93.25%,the replay rate was 2.97%and the missing seeding rate was 3.78%under the condition with the inclination angle of seed tube end of 32.28°,the forward speed of the machine of 6.89 km/h and the operating speed of the metering disc of 28.67 r/min,which can meet the requirements of relevant national standards.展开更多
基金National Natural Science Foundation of China(U19B2003,21706177,21975174)Foundation Supported by China Petroleum&Chemical Corporation(121014-2)。
文摘Synthesis of ZSM-5 zeolite typically utilizes small molecule polyamines or quaternary ammonium salts as organic structure guiding agent(OSDA).By contrast,the OSDA-free hydrothermal synthesis system eliminates the use of organic templates and the subsequent calcination procedure.This not only reduces the cost of synthesis,but also prevents environmental pollution from the combustion of organic templates,representing an eco-friendly approach.Despite this,literature suggests that even so-called template-free synthesis systems often involve trace amount of organic substances like alcohol.In the present work,a calcined commercial ZSM-5 zeolite was served as seed,with sodium aluminate as aluminum source and silica sol as silicon source,ensuring an entirely template-free synthesis system.Polycrystalline ZSM-5 aggregates consisted of rod-like nanocrystals were successfully prepared in the completely OSDA-free system.Effects of the Si/Al ratio in ZSM-5 seed,dosage and crystallization conditions such as crystallization temperature and crystallization time on ZSM-5 synthesis were investigated.The results show that a highly crystallinity ZSM-5 aggregate consisting of primary nano-sized crystals less than 100 nm is produced from a gel precursor with 5.6%(in mass)seed after hydrothermal treatment for 48 h.Furthermore,the Si/Al ratio in ZSM-5 seed has little effect on the topological structure and pore structure of the synthesized samples.However,the seeds with a low Si/Al ratio facilitate faster crystallization of zeolite and enhance the acidity,especially the strong acid centers,of the catalyst.The catalytic performance of the synthesized polycrystalline ZSM-5 was evaluated during dehydration of methanol and compared with a commercial reference ZSM-5r.The results exhibit that as compared with the reference catalyst,the fabricated sample has a longer catalytic lifetime(16 h vs 8 h)attributed to its hierarchical pores derived from the loosely packed primary nanoparticles.Additionally,the prepared polycrystalline catalyst also exhibits a higher aromatics selectivity(28.1%-29.8%vs 26.5%).
基金financially supported by the National Natural Science Foundation(Grant No.52275246)the Key Research and Development Plan Project of Heilongjiang Province(Grant No.2022ZX05B02).
文摘Seeding is an important part of improving corn yield.Currently,seed guide tubes are mostly used as transport devices.But the existing seed guide tubes cannot meet the requirements or achieve the goal of fixing the seed falling trajectory.A seed collision phenomenon occurs occasionally.So,in response to the problems that the seeds and seed guide tube collide or bounce under high speed operation,which results in a lower sowing qualification rate and poor spacing uniformity,a seed receiving and conveying system comprising a belt-type high-speed corn seed guiding device was designed and optimized,to meet the needs of high-speed precision sowing operations and improve the spacing uniformity.The factors affecting the seed conveying performance were obtained by analyzing the mechanical properties of the seeds at various movement stages.These factors were the number of seed cavities between adjacent seeds,the forward speed,the height from the ground,and the installation angle.Single factor simulation experiments were conducted by selecting the paddle spacing as the test factor and using the pass rate,reseeding rate,omission rate and coefficient of variation as the evaluation indexes to investigate the influence of the paddle spacing on the seed guide performance of the device and further determine the structural parameters of the paddle belt.Orthogonal rotation combination tests of three factors and five levels were also conducted through bench testing.Then the test outcomes were optimized.The results indicated that the best results were obtained when the number of seed cavity intervals between adjacent seeds was 5.16,the installation angle was 79.40°,and the height from the ground was 31.84 mm.At this time,the qualified rate was 98.49%,the repeated sowing rate was 0.48%,the missed sowing rate was 1.03%,and the coefficient of variation was 6.80%.Experiments were used to validate the optimization results,and all of the obtained index data satisfied the criteria for accurate and quick corn sowing.The study’s findings can serve as a theoretical foundation for a belt-type high-speed corn seed guiding device optimization test.
基金supported by the National Institute of Neurological Disorders and Stroke of the National Institutes of Health (No. R01NS102360)。
文摘It was hypothesized that mesenchymal stem cells(MSCs) could provide necessary trophic factors when seeded onto the surfaces of commonly used nerve graft substitutes. We aimed to determine the gene expression of MSCs when influenced by Avance■ Nerve Grafts or Neura Gen■ Nerve Guides. Human adipose-derived MSCs were cultured and dynamically seeded onto 30 Avance■ Nerve Grafts and 30 Neura Gen■ Nerve Guides for 12 hours. At six time points after seeding, quantitative polymerase chain reaction analyses were performed for five samples per group. Neurotrophic [nerve growth factor(NGF), glial cell line-derived neurotrophic factor(GDNF), pleiotrophin(PTN), growth associated protein 43(GAP43) and brain-derived neurotrophic factor(BDNF)], myelination [peripheral myelin protein 22(PMP22) and myelin protein zero(MPZ)], angiogenic [platelet endothelial cell adhesion molecule 1(PECAM1/CD31) and vascular endothelial cell growth factor alpha(VEGFA)], extracellular matrix(ECM) [collagen type alpha I(COL1A1), collagen type alpha III(COL3A1), Fibulin 1(FBLN1) and laminin subunit beta 2(LAMB2)] and cell surface marker cluster of differentiation 96(CD96) gene expression was quantified. Unseeded Avance■ Nerve Grafts and Neura Gen■ Nerve Guides were used to evaluate the baseline gene expression, and unseeded MSCs provided the baseline gene expression of MSCs. The interaction of MSCs with the Avance■ Nerve Grafts led to a short-term upregulation of neurotrophic(NGF, GDNF and BDNF), myelination(PMP22 and MPZ) and angiogenic genes(CD31 and VEGFA) and a long-term upregulation of BDNF, VEGFA and COL1A1. The interaction between MSCs and the Neura Gen■ Nerve Guide led to short term upregulation of neurotrophic(NGF, GDNF and BDNF) myelination(PMP22 and MPZ), angiogenic(CD31 and VEGFA), ECM(COL1A1) and cell surface(CD96) genes and long-term upregulation of neurotrophic(GDNF and BDNF), angiogenic(CD31 and VEGFA), ECM genes(COL1A1, COL3A1, and FBLN1) and cell surface(CD96) genes. Analysis demonstrated MSCs seeded onto Neura Gen■ Nerve Guides expressed significantly higher levels of neurotrophic(PTN), angiogenic(VEGFA) and ECM(COL3A1, FBLN1) genes in the long term period compared to MSCs seeded onto Avance■ Nerve Grafts. Overall, the interaction between human MSCs and both nerve graft substitutes resulted in a significant upregulation of the expression of numerous genes important for nerve regeneration over time. The in vitro interaction of MSCs with the Neura Gen■ Nerve Guide was more pronounced, particularly in the long term period(> 14 days after seeding). These results suggest that MSC-seeding has potential to be applied in a clinical setting, which needs to be confirmed in future in vitro and in vivo research.
文摘Endoscopic ultrasonography-guided fine needle aspiration(EUS-FNA) is a useful and relatively safe tool for the diagnosis and staging of pancreatic cancer. However, there have recently been several reports of tumor seeding after EUS-FNA of adenocarcinomas. A 78-year-old man was admitted to our hospital due to upper gastric pain. Examinations revealed a 20 mm mass in the pancreatic body, for which EUS-FNA was performed. The cytology of the lesion was adenocarcinoma, and the stage of the cancer was T3N0M0. The patient underwent surgery with curative intent, followed by adjuvant chemotherapy with S-1. An enlarging gastric submucosal tumor was found on gastroscopy at 28 mo after surgery accompanied by a rising level of CA19-9. Biopsy result was adenocarcinoma, consistent with a pancreatic primary tumor. Tumor seeding after EUS-FNA was strongly suspected. The patient underwent surgical resection of the gastric tumor with curative intent. The pathological result of the resected gastric specimen was adenocarcinoma with a perfectly matched mucin special stain result with the previously resected pancreatic cancer. This is the first case report of tumor seeding after EUS-FNA which was surgically resected and inspected pathologically.
文摘We reported two cases of liver metastasis with portal vein tumor thrombus that developed after liver transplantation for hepato cellular carcinoma (HCC). Both the patients were women aged 43 and 55 years, who had liver metastasis and portal vein tumor thrombus formation after liver transplantations for HCC.
基金sponsored by the Natural Science Foundation of Shandong Province(Grant No.ZR202111230084)Qingdao Science and Technology Demonstration and Guidance Project(Grant No.2237xdny17nsh)+2 种基金Shandong Science and Technology SMEs Innovation Capacity Improvement Project(Grant No.2021TSGC 1428)Key R&D Plan of Shandong Province(Grant No.2019GNC106056)Key R&D Plan of Shandong Province(Grant No.2017NC212006)and Qingdao Minsheng Science and Technology Plan(Grant No.173352nsh).
文摘To meet the requirement of“zero speed”for precision sowing,the instantaneous speed of seed falling into the seed bed should be equal to the opposite direction of the forward speed of the machine.Through analyzing the principle of seed guiding process,the main influencing factors affecting the contour curve of the seed tube were determined to plan the seed guiding path and realize zero speed seed delivery.With the help of EDEM simulation software,taking the inclination angle of seed tube end,operating speed of the metering disc,and machine’s forward speed as the experimental factors,a single-factor simulation test was conducted with the pass rate,replay rate,and missing seeding rate as experimental indexes.The results of the simulation test showed that the range of inclination angle of the seed tube end was 23°-40°,the range of machine forward speed was 5-9 km/h,and the range of operating speed of the metering disc was 15-40 r/min.The quadratic orthogonal rotation combination test method was carried out using the bench test,and a mathematical regression model was established between experimental factors and experimental indexes to determine the priority order of factors,and the best combination of factors.The test results showed that the pass rate was 93.25%,the replay rate was 2.97%and the missing seeding rate was 3.78%under the condition with the inclination angle of seed tube end of 32.28°,the forward speed of the machine of 6.89 km/h and the operating speed of the metering disc of 28.67 r/min,which can meet the requirements of relevant national standards.