Different technical conditions in the process of solid-state fermentation of red yeast rice has a certain impact on the production of acid-form and lactone form Monacolin K.When cultured in the loose rice medium,the a...Different technical conditions in the process of solid-state fermentation of red yeast rice has a certain impact on the production of acid-form and lactone form Monacolin K.When cultured in the loose rice medium,the acid form Monacolin K accounts for 50%-60%;while the acid form Monacolin K accounts for 80%-90%cultured in the consolidated rice medium.And we also found that the proportion of the acid form and lactone form Monacolin K will decrease with the increase of fermentation time.展开更多
目的:分析鉴定实验室自制的功能红曲菌的固态发酵物中的主要莫纳可林类物质。方法:首先通过固态发酵制得功能红曲粉,然后分别使用极性不同的4种溶剂(蒸馏水、乙醇、乙酸乙酯和正己烷)超声提取提取功能红曲粉,再通过减压旋蒸及冷冻干燥...目的:分析鉴定实验室自制的功能红曲菌的固态发酵物中的主要莫纳可林类物质。方法:首先通过固态发酵制得功能红曲粉,然后分别使用极性不同的4种溶剂(蒸馏水、乙醇、乙酸乙酯和正己烷)超声提取提取功能红曲粉,再通过减压旋蒸及冷冻干燥制得提取物粉末。通过UPLC-Q-TOF-MS对功能红曲粉的不同溶剂提取物的化学成分进行定性分析与比较。采用Agilent Eclipse Plus-C18 RRHD色谱柱(2.1 mm x 100 mm,1.8μm),以乙腈-0.1%甲酸水为流动相进行梯度洗脱。质谱分析采用电喷雾正离子全扫描模式采集,扫描范围为m/z 100-1500Da。结论:本文通过UPLC-Q-TOF-MS对功能红曲粉的不同溶剂提取物的化学成分进行定性分析与比较。鉴定出17种主要的莫纳可林类物质。该方法快速、可靠、高效,能较全面地反映功能红曲粉的的提取物中的主要莫纳可林类物质,可为深入研究功能红曲粉中的药效物质提供参考。展开更多
文摘Different technical conditions in the process of solid-state fermentation of red yeast rice has a certain impact on the production of acid-form and lactone form Monacolin K.When cultured in the loose rice medium,the acid form Monacolin K accounts for 50%-60%;while the acid form Monacolin K accounts for 80%-90%cultured in the consolidated rice medium.And we also found that the proportion of the acid form and lactone form Monacolin K will decrease with the increase of fermentation time.
文摘目的:分析鉴定实验室自制的功能红曲菌的固态发酵物中的主要莫纳可林类物质。方法:首先通过固态发酵制得功能红曲粉,然后分别使用极性不同的4种溶剂(蒸馏水、乙醇、乙酸乙酯和正己烷)超声提取提取功能红曲粉,再通过减压旋蒸及冷冻干燥制得提取物粉末。通过UPLC-Q-TOF-MS对功能红曲粉的不同溶剂提取物的化学成分进行定性分析与比较。采用Agilent Eclipse Plus-C18 RRHD色谱柱(2.1 mm x 100 mm,1.8μm),以乙腈-0.1%甲酸水为流动相进行梯度洗脱。质谱分析采用电喷雾正离子全扫描模式采集,扫描范围为m/z 100-1500Da。结论:本文通过UPLC-Q-TOF-MS对功能红曲粉的不同溶剂提取物的化学成分进行定性分析与比较。鉴定出17种主要的莫纳可林类物质。该方法快速、可靠、高效,能较全面地反映功能红曲粉的的提取物中的主要莫纳可林类物质,可为深入研究功能红曲粉中的药效物质提供参考。
