Acridone derivatives are widespread in nature and known to be capable of having a variety of biological activities, but there is not any information on the acridone series compounds in relation to higher fungi, includ...Acridone derivatives are widespread in nature and known to be capable of having a variety of biological activities, but there is not any information on the acridone series compounds in relation to higher fungi, including edible mushrooms available in literature at all. Research into the character of the mode of action of these substances on various living systems is necessary to elucidate the possible unfavorable biological consequences and improve measures on their ecological safety. In this work, the effect of acridone-N-acetic acid on mushroom mycelial growth was tested and the culture of basidiomycete Lentinula edocles （shiitake） has been used. The influence of acridone additive upon the fungal mycelium growth on liquid （submerged） and agar media was examined within the wide concentration range of acridone carboxy-derivative. The results obtained testify to the relative ecological safety of these substances for mushroom organism, and to the mycelial growth promoting capability of acridone-N-acetic acid at favorable concentrations, both under the solid-phase and liquid-phase culture conditions. In fact, the very first step toward the investigation into the systems ＂macromycete-acridone series compound＂ has been made.展开更多
As an insufficiently utilized energy resource,oil shale is conducive to the formation of characteristic microbial communities due to its special geological origins.However,little is known about fungal diversity in oil...As an insufficiently utilized energy resource,oil shale is conducive to the formation of characteristic microbial communities due to its special geological origins.However,little is known about fungal diversity in oil shale.Polymerase chain reaction cloning was used to construct the fungal ribosomal deoxyribonucleic acid internal transcribed spacer（r DNA ITS）clone libraries of Huadian Mine in Jilin Province,Maoming Mine in Guangdong Province,and Fushun Mine in Liaoning Province.Pure culture and molecular identification were applied for the isolation of cultivable fungi in fresh oil shale of each mine.Results of clone libraries indicated that each mine had over 50% Ascomycota（58.4%–98.9%）and 1.1%–13.5%unidentified fungi.Fushun Mine and Huadian Mine had 5.9% and 28.1% Basidiomycota,respectively.Huadian Mine showed the highest fungal diversity,followed by Fushun Mine and Maoming Mine.Jaccard indexes showed that the similarities between any two of three fungal communities at the genus level were very low,indicating that fungi in each mine developed independently during the long geological adaptation and formed a community composition fitting the environment.In the fresh oil-shale samples of the three mines,cultivable fungal phyla were consistent with the results of clone libraries.Fifteen genera and several unidentified fungi were identified as Ascomycota and Basidiomycota using pure culture.Penicillium was the only genus found in all three mines.These findings contributed to gaining a clear understanding of current fungal resources in major oil-shale mines in China and provided useful information for relevant studies on isolation of indigenous fungi carrying functional genes from oil shale.展开更多
文摘Acridone derivatives are widespread in nature and known to be capable of having a variety of biological activities, but there is not any information on the acridone series compounds in relation to higher fungi, including edible mushrooms available in literature at all. Research into the character of the mode of action of these substances on various living systems is necessary to elucidate the possible unfavorable biological consequences and improve measures on their ecological safety. In this work, the effect of acridone-N-acetic acid on mushroom mycelial growth was tested and the culture of basidiomycete Lentinula edocles （shiitake） has been used. The influence of acridone additive upon the fungal mycelium growth on liquid （submerged） and agar media was examined within the wide concentration range of acridone carboxy-derivative. The results obtained testify to the relative ecological safety of these substances for mushroom organism, and to the mycelial growth promoting capability of acridone-N-acetic acid at favorable concentrations, both under the solid-phase and liquid-phase culture conditions. In fact, the very first step toward the investigation into the systems ＂macromycete-acridone series compound＂ has been made.
基金supported by the National Natural Science Foundation of China(No.51204055)the Fundamental Research Funds for the Central Universities(No.N130420002)the China Postdoctoral Science Foundation(20100481205)
文摘As an insufficiently utilized energy resource,oil shale is conducive to the formation of characteristic microbial communities due to its special geological origins.However,little is known about fungal diversity in oil shale.Polymerase chain reaction cloning was used to construct the fungal ribosomal deoxyribonucleic acid internal transcribed spacer（r DNA ITS）clone libraries of Huadian Mine in Jilin Province,Maoming Mine in Guangdong Province,and Fushun Mine in Liaoning Province.Pure culture and molecular identification were applied for the isolation of cultivable fungi in fresh oil shale of each mine.Results of clone libraries indicated that each mine had over 50% Ascomycota（58.4%–98.9%）and 1.1%–13.5%unidentified fungi.Fushun Mine and Huadian Mine had 5.9% and 28.1% Basidiomycota,respectively.Huadian Mine showed the highest fungal diversity,followed by Fushun Mine and Maoming Mine.Jaccard indexes showed that the similarities between any two of three fungal communities at the genus level were very low,indicating that fungi in each mine developed independently during the long geological adaptation and formed a community composition fitting the environment.In the fresh oil-shale samples of the three mines,cultivable fungal phyla were consistent with the results of clone libraries.Fifteen genera and several unidentified fungi were identified as Ascomycota and Basidiomycota using pure culture.Penicillium was the only genus found in all three mines.These findings contributed to gaining a clear understanding of current fungal resources in major oil-shale mines in China and provided useful information for relevant studies on isolation of indigenous fungi carrying functional genes from oil shale.
