Objective:To evaluate in ritro antimicrobial activities of selected 58 ethno-medicinal plant extracts with a view to assess their therapeutic potential.Methods:A total of 58 traditional Chinese medicinal plants were c...Objective:To evaluate in ritro antimicrobial activities of selected 58 ethno-medicinal plant extracts with a view to assess their therapeutic potential.Methods:A total of 58 traditional Chinese medicinal plants were carefully selected based on the literature review and their traditional use.The antimicrobial activities of ethanol extracts of these medicinal plants were tested against fungi(Aspergillus funigaius),yeast(Candida albicans),gram-negative(Acirelobacter haumannii and Pseudornnruis aeruginosa)and gram-positive bacteria(Staphglococcus aureus).The activities were tested at three different concentrations of 1.00,0.10 and 0.01 mg/mL.The data was analysed using Gene data Screener program.Results:The measured antimicrobial activities indicated that out of the 58 plant extracts,15 extracts showed anti-fungal activity and 23 extracts exhibited anti-bacterial activity.Eight plant extracts have exhibited both anti-bacterial and anti-fungal activities.For instance,Eucommia ulmoides,Pohgonum cuspidcrtum,Poria cocas and Uncaria rhineophylla showed activity against both bacterial and fungal strains,indicating their broad spectrum of activity.Conclusions:The results revealed that the ethanol extracts of 30 plants out of the selected 58 possess significant antimicrobial activities.It is interesting to note that the findings from the current study are consistent with the traditional use.A clear correlation has also been found between the antimicrobial activity and the flavonoid content of the plant extracts which is in agreement with the literature.Hence.the results presented here can be used to guide the selection of potential plant species for the isolation and structure elucidation of novel antimicrobial compounds in order to establish the structure-activity relationship.This in turn is expected to lead the way to the discovery of novel antimicrobial agents for therapeutic use.展开更多
Objective: To evaluate the in vitro antimicrobial property of three different partitioned extracts(petroleum ether, ethanol and water) prepared from some fungal mycelia.Methods: Seven fungal mycelia were prepared, ini...Objective: To evaluate the in vitro antimicrobial property of three different partitioned extracts(petroleum ether, ethanol and water) prepared from some fungal mycelia.Methods: Seven fungal mycelia were prepared, initially extracted with acidified ethanol(0.2 mol/L HCl in 80% ethanol), yielding the raw crude extracts. The obtained extracts were then further partitioned with petroleum ether(F1), ethanol(F2) and water(F3). All the fractions were tested for antimicrobial activity using the disc diffusion assay.Results: Our data showed that all the fractions could inhibit the testing bacteria.However, the inhibitory activity was found to be dependent on(i) the fungal strains used;(ii) the solvent extracted; and(iii) the testing bacteria assayed. In general, the ethanolic extracts(F2) derived from all fungi displayed highest inhibitory activity against the testing bacteria except for Chaetomium sp.Conclusions: The findings of the present study concluded that the extracts prepared from the fungal mycelia had the bioactive compounds with antibacterial property. This study is a pioneering work and further study should be carried out for development of the new drug leads.展开更多
Objective: To investigate the effect of temperature and pressure on supercritical CO_2 extraction of Triognella foenum graecum Linn seeds, to determine the optimal condition which leads to highest percentage of the ac...Objective: To investigate the effect of temperature and pressure on supercritical CO_2 extraction of Triognella foenum graecum Linn seeds, to determine the optimal condition which leads to highest percentage of the accumulative yield and revealing the chemical composition of supercritical CO_2 extract.Methods: Temperatures in the range of 40–60C and pressures in the range of 10–25 MPa were used. FTIR and GC–MS analysis were used to detect the bioactive compounds present in the extract. The broth dilution method and slope method were used to evaluate the anti-microbial and anti-tuberculosis activities and the in vitro anti-malarial assay was carried out according to the micro assay protocol of Rieckmann and his coworkers.Results: The temperature was more affected than the pressure on the extraction performance and the highest yield of the extract(3.111%) was attained at 60C and 10 MPa.FTIR and GC–MS showed that the chemical composition of the extract included conjugated linoleic acid methyl ester as the major active principle(with concentration of72.28%), followed by saturated fatty acid methyl esters(16.03%), steroids(8.09%) and organic siloxane compound(3.61%). The extract showed moderate anti-bacterial activity with MIC values 100, 250, 125 mg/m L towards Escherichia coli, Staphylococcus aureus and Streptococcus pyogenus respectively. It exhibited high inhibition effect towards the fungi Candida albican with MFC value(250 mg/m L). The extract had low antituberculosis activity with MIC value(100 mg/m L) and comparable MIC value(0.29 mg/m L) towards Plasmodium flaciparum.Conclusions: Supercritical CO_2 extraction as alternate and green technology is performed successfully to extract the bioactive compounds from the seeds of T. foenum graecum Linn and it is concluded that this extract can be used as an alternate source of synthetic anti-biotic drugs.展开更多
目的探索和评价提取致病性真菌DNA的简便方法。方法分别用6种方法(液氮冻融、-70℃冻融、溶胞酶消解、超声破碎、高盐溶液、尿素溶液)裂解真菌细胞壁,再用市售DNeasy B lood&Tissue K it试剂盒提取DNA,最后通过对提取物进行比色定量...目的探索和评价提取致病性真菌DNA的简便方法。方法分别用6种方法(液氮冻融、-70℃冻融、溶胞酶消解、超声破碎、高盐溶液、尿素溶液)裂解真菌细胞壁,再用市售DNeasy B lood&Tissue K it试剂盒提取DNA,最后通过对提取物进行比色定量和PCR检测评价各种提取方法的优弊。结果酶消解破壁法提取真菌DNA效率最高,重复性良好。模拟全血标本中白色念珠菌检测限为102个孢子,烟曲霉及新型隐球菌检测限为103个孢子。结论酶消解破壁法提取真菌DNA在6种方法中效率最高,可用于临床常见真菌感染的PCR快速诊断。展开更多
基金funded by the University of Western Sydney and the Fundacion MEDINAa public-private partnership of Merck Sharp&Dohme de Espana S.A./Universidad de Granada/Junta de Andalucia
文摘Objective:To evaluate in ritro antimicrobial activities of selected 58 ethno-medicinal plant extracts with a view to assess their therapeutic potential.Methods:A total of 58 traditional Chinese medicinal plants were carefully selected based on the literature review and their traditional use.The antimicrobial activities of ethanol extracts of these medicinal plants were tested against fungi(Aspergillus funigaius),yeast(Candida albicans),gram-negative(Acirelobacter haumannii and Pseudornnruis aeruginosa)and gram-positive bacteria(Staphglococcus aureus).The activities were tested at three different concentrations of 1.00,0.10 and 0.01 mg/mL.The data was analysed using Gene data Screener program.Results:The measured antimicrobial activities indicated that out of the 58 plant extracts,15 extracts showed anti-fungal activity and 23 extracts exhibited anti-bacterial activity.Eight plant extracts have exhibited both anti-bacterial and anti-fungal activities.For instance,Eucommia ulmoides,Pohgonum cuspidcrtum,Poria cocas and Uncaria rhineophylla showed activity against both bacterial and fungal strains,indicating their broad spectrum of activity.Conclusions:The results revealed that the ethanol extracts of 30 plants out of the selected 58 possess significant antimicrobial activities.It is interesting to note that the findings from the current study are consistent with the traditional use.A clear correlation has also been found between the antimicrobial activity and the flavonoid content of the plant extracts which is in agreement with the literature.Hence.the results presented here can be used to guide the selection of potential plant species for the isolation and structure elucidation of novel antimicrobial compounds in order to establish the structure-activity relationship.This in turn is expected to lead the way to the discovery of novel antimicrobial agents for therapeutic use.
基金Supported by“Operational Program Prague–Competitiveness”(CZ.2.16/3.1.00/24503)the“National Program of Sustainability I”-NPU I(LO1601-No.:MSMT-43760/2015)from the University of Chemistry and Technology,Czech Republic
文摘Objective: To evaluate the in vitro antimicrobial property of three different partitioned extracts(petroleum ether, ethanol and water) prepared from some fungal mycelia.Methods: Seven fungal mycelia were prepared, initially extracted with acidified ethanol(0.2 mol/L HCl in 80% ethanol), yielding the raw crude extracts. The obtained extracts were then further partitioned with petroleum ether(F1), ethanol(F2) and water(F3). All the fractions were tested for antimicrobial activity using the disc diffusion assay.Results: Our data showed that all the fractions could inhibit the testing bacteria.However, the inhibitory activity was found to be dependent on(i) the fungal strains used;(ii) the solvent extracted; and(iii) the testing bacteria assayed. In general, the ethanolic extracts(F2) derived from all fungi displayed highest inhibitory activity against the testing bacteria except for Chaetomium sp.Conclusions: The findings of the present study concluded that the extracts prepared from the fungal mycelia had the bioactive compounds with antibacterial property. This study is a pioneering work and further study should be carried out for development of the new drug leads.
基金acknowledge to Hodeidah University, Yemen for supporting this work
文摘Objective: To investigate the effect of temperature and pressure on supercritical CO_2 extraction of Triognella foenum graecum Linn seeds, to determine the optimal condition which leads to highest percentage of the accumulative yield and revealing the chemical composition of supercritical CO_2 extract.Methods: Temperatures in the range of 40–60C and pressures in the range of 10–25 MPa were used. FTIR and GC–MS analysis were used to detect the bioactive compounds present in the extract. The broth dilution method and slope method were used to evaluate the anti-microbial and anti-tuberculosis activities and the in vitro anti-malarial assay was carried out according to the micro assay protocol of Rieckmann and his coworkers.Results: The temperature was more affected than the pressure on the extraction performance and the highest yield of the extract(3.111%) was attained at 60C and 10 MPa.FTIR and GC–MS showed that the chemical composition of the extract included conjugated linoleic acid methyl ester as the major active principle(with concentration of72.28%), followed by saturated fatty acid methyl esters(16.03%), steroids(8.09%) and organic siloxane compound(3.61%). The extract showed moderate anti-bacterial activity with MIC values 100, 250, 125 mg/m L towards Escherichia coli, Staphylococcus aureus and Streptococcus pyogenus respectively. It exhibited high inhibition effect towards the fungi Candida albican with MFC value(250 mg/m L). The extract had low antituberculosis activity with MIC value(100 mg/m L) and comparable MIC value(0.29 mg/m L) towards Plasmodium flaciparum.Conclusions: Supercritical CO_2 extraction as alternate and green technology is performed successfully to extract the bioactive compounds from the seeds of T. foenum graecum Linn and it is concluded that this extract can be used as an alternate source of synthetic anti-biotic drugs.
文摘目的探索和评价提取致病性真菌DNA的简便方法。方法分别用6种方法(液氮冻融、-70℃冻融、溶胞酶消解、超声破碎、高盐溶液、尿素溶液)裂解真菌细胞壁,再用市售DNeasy B lood&Tissue K it试剂盒提取DNA,最后通过对提取物进行比色定量和PCR检测评价各种提取方法的优弊。结果酶消解破壁法提取真菌DNA效率最高,重复性良好。模拟全血标本中白色念珠菌检测限为102个孢子,烟曲霉及新型隐球菌检测限为103个孢子。结论酶消解破壁法提取真菌DNA在6种方法中效率最高,可用于临床常见真菌感染的PCR快速诊断。
