The incidence of the airborne fungal spores was determined in the air of subalpine zone of the Karkonosze Mountains and of the Izerskie Mountains in the borderland between Poland and the Czech Republic. The experiment...The incidence of the airborne fungal spores was determined in the air of subalpine zone of the Karkonosze Mountains and of the Izerskie Mountains in the borderland between Poland and the Czech Republic. The experiment was conducted in2011 and 2012 at three to four week intervals from May to October. Air samples were taken from three locations in the Karkonosze Mts. and one from the Izerskie Mts. To examine the air, the Air Ideal 3P sampler and acidified PDA medium were used. The results show that Cladosporium cladosporioides was the most abundant spore type in all the sampling locations(up to 30%), followed by Alternaria alternata(16%–20%), Fusarium(up to 10%) and Sclerotinia sclerotiorium(up to 6%). The lower spore counts were recorded in May samples, compared to the other months. In this case the snow cover, that was still present in the area at the beginning of May,may be the reason for the lower, compared to June,July and August samplings, CFU(Colony Forming Unit) counts in that month.. The influx of air masses from SE, S and SW sectors in the area under study may affect dissemination of the plant-pathogenic fungi from the Czech Republic and from the South of Europe in general.展开更多
Pastoralism is considered a crucial factor in the eventual year-round occupation of high-altitude regions(>3000 m asl)of the Qinghai-Tibetan Plateau,and the northeastern Qinghai-Tibetan Plateau(NE-QTP)was an import...Pastoralism is considered a crucial factor in the eventual year-round occupation of high-altitude regions(>3000 m asl)of the Qinghai-Tibetan Plateau,and the northeastern Qinghai-Tibetan Plateau(NE-QTP)was an important corridor for early human occupation.We analyzed fossil fungal spore records for the last 8500 years from a high resolution aeolian section at Langgeri(LGR)on the NE-QTP.Thirty-two fungal spore types were identified in the LGR section,including seven coprophilous types.We combined analysis of coprophilous fungal spores,Cyperaceae,Artemisia,and Hippophae pollen,and the charcoal>50µm fraction to explore the timing and controls of pastoralism on the NE-QTP since the middle Holocene.Pastoralism commenced at LGR shortly before ca.5.5 ka and gradually increased between ca.5.5–3.5 ka,but markedly intensified after ca.2.2 ka,with three periods of growth at ca.2.1–1.9,1.4–1.2,and 0.6–0 ka,and a decline at ca.1.1–0.6 ka.The timing of changes in pastoral activity on the NE-QTP based on the coprophilous fungal spore record is supported by regional archeology and historical documents.Technological and political developments,rather than climate change,played key roles in the long-term fluctuations of regional pastoralism on the NE-QTP in the late Holocene.展开更多
Mycological tools to estimate the effects of diverse land-use practices on fungal diversity are scarce,because of poor knowledge of the taxonomic diversity of tropical fungi and their response to anthropogenic habitat...Mycological tools to estimate the effects of diverse land-use practices on fungal diversity are scarce,because of poor knowledge of the taxonomic diversity of tropical fungi and their response to anthropogenic habitat change.Here,we investigate assemblages of fungal spores,recently deposited in the bottom sediments of 24 small crater lakes in western Uganda,to assess the relationship between the local richness of fungi and environmental variation in the crater basin along regional gradients of natural vegetation and land use.We recovered~9500 fungal spore specimens,which could be attributed to 216 morphotypes.Using an information-theoretic approach based on the corrected Akaike Information Criterion(AICc),we determined the environmental factors which best explained variation in the diversity of fungal spores among three datasets:(i)the full set of 24 crater basins,(ii)the subset of 22 basins with freshwater lakes,and(iii)the subset of 17 basins partly or completely in agricultural use(cropland,fallow land,pasture and plantation).In these 17 human-impacted crater basins our results revealed a negative relationship between fungal spore richness and the areal fraction of basins in agricultural use.However,this detrimental effect of land use on fungal spore richness was not apparent across the full set of both disturbed and(presently)undisturbed basins.This was due to large variation in fungal spore richness among the undisturbed basins covered either with forest or savannah vegetation,probably resulting from site-specific controls on fungal habitat diversity,such as climatic moisture balance and the composition of natural and/or secondary vegetation.The land-use effects on fungal spore diversity,as documented in this study,suggest that communities of tropical fungi progressively exposed to land-use practices are threatened by species loss.Hence,our study demonstrates the need to develop conservation strategies mitigating the impacts of agriculture on the biodiversity of tropical fungi.展开更多
Yellow rust(Puccinia striiformis f.sp.Tritici)is a frequently occurring fungal disease of winter wheat(Triticum aestivum L.).During yellow rust infestation,fungal spores appear on the surface of the leaves as yellow a...Yellow rust(Puccinia striiformis f.sp.Tritici)is a frequently occurring fungal disease of winter wheat(Triticum aestivum L.).During yellow rust infestation,fungal spores appear on the surface of the leaves as yellow and narrow stripes parallel to the leaf veins.We analyzed the effect of the fungal spores on the spectra of the diseased leaves to find a band sensitive to yellow rust and established a new vegetation index called the yellow rust spore index(YRSI).The estimation accuracy and stability were evaluated using two years of leaf spectral data,and the results were compared with eight indices commonly used for yellow rust detection.The results showed that the use of the YRSI ranked first for estimating the disease ratio for the 2017 spectral data(R^(2)=0.710,RMSE=0.097)and outperformed the published indices(R^(2)=0.587,RMSE=0.120)for the validation using the 2002 spectral data.The random forest(RF),k-nearest neighbor(KNN),and support vector machine(SVM)algorithms were used to test the discrimination ability of the YRSI and the eight commonly used indices using a mixed dataset of yellow-rust-infested,healthy,and aphid–infested wheat spectral data.The YRSI provided the best performance.展开更多
Isolating cellulase-secreting microbes followed-by screening their cellulolytic activities has been an essential approach to discover novel and potential cellulases for cellulolytic industrial applications. This study...Isolating cellulase-secreting microbes followed-by screening their cellulolytic activities has been an essential approach to discover novel and potential cellulases for cellulolytic industrial applications. This study was aimed to explore competitive exoglucanases by screening avicelase activities for 92 fungal strains isolated from environmental airborne-fungal-spore samples. Results showed that an isolated fungal strain numbered 58 exhibited the best avicelase activity of 0.209 U/mL when cultured for six days at pH 5.0 - 5.3 and 25℃ - 27℃, and was lately identified as a yeast strain of Meyerozyma sp. (96% ITS fragment similar with Meyerozyma caribbica, HG970748). Based on amino acid sequences revealed from LC/MS/MS, the target exoglucanase was identical to 1,4-beta-D-glucan cellobiohydrolases and was named Mc-CBHI which had optimal avicelase reaction conditions of pH 5 and 70℃ and could remain fairly stable after 4hr incubation at acid conditions (pH 3 - 5) or wide temperature ranges (30℃ - 80℃). Additionally, the Mc-CBHI (~70 kDa and ~3.6% of crude enzyme) had specific FPase and avicelase activities of 0.179 U/mg and 0.126 U/mg, respectively (which were about 40% - 50% activities of a commercial cellulase Accellerase-1000). These results demonstrated that the newly-found Mc-CBHI could become one of potential exoglucanase resources for related cellulolytic industrial applications.展开更多
Recently, the pharmacological study of Ganoderma spores and active components has be-come a focus of attention in the world. The present reviewis based on the auctorial research on Ganoder-maspores. It involves pharma...Recently, the pharmacological study of Ganoderma spores and active components has be-come a focus of attention in the world. The present reviewis based on the auctorial research on Ganoder-maspores. It involves pharmacological effects of Ganodermaspores and its active components, includingimmunomodulating effect, antitumor activity and its mechanismin vivoandin vitro, liver-protectiveeffect, gastric ulcer preventing effect, serum glucose and blood fat depressing effects, anti-hypoxia andscavenging free redical, etc. The possible problems and their solutions in this research area are also dis-cussed.展开更多
基金owe a debt of gratitude to the Department of Plant Protection, Wroclaw University of Environmental and Life Sciences for financing the project
文摘The incidence of the airborne fungal spores was determined in the air of subalpine zone of the Karkonosze Mountains and of the Izerskie Mountains in the borderland between Poland and the Czech Republic. The experiment was conducted in2011 and 2012 at three to four week intervals from May to October. Air samples were taken from three locations in the Karkonosze Mts. and one from the Izerskie Mts. To examine the air, the Air Ideal 3P sampler and acidified PDA medium were used. The results show that Cladosporium cladosporioides was the most abundant spore type in all the sampling locations(up to 30%), followed by Alternaria alternata(16%–20%), Fusarium(up to 10%) and Sclerotinia sclerotiorium(up to 6%). The lower spore counts were recorded in May samples, compared to the other months. In this case the snow cover, that was still present in the area at the beginning of May,may be the reason for the lower, compared to June,July and August samplings, CFU(Colony Forming Unit) counts in that month.. The influx of air masses from SE, S and SW sectors in the area under study may affect dissemination of the plant-pathogenic fungi from the Czech Republic and from the South of Europe in general.
基金supported by the National Natural Science Foundation of China(Grant No.41877455)the Science and Technology Innovation Platform Project of Qinghai Province(Grant No.2020-ZJ-T06)+2 种基金the Open Foundation of State Key Laboratory of Paleobiology and Stratigraphy(Nanjing Institute of Geology and Palaeontology,CASGrant No.183123)the Second Tibetan Plateau Scientific Expedition and Research Program(STEP)(Grant No.2019QZKK0805).
文摘Pastoralism is considered a crucial factor in the eventual year-round occupation of high-altitude regions(>3000 m asl)of the Qinghai-Tibetan Plateau,and the northeastern Qinghai-Tibetan Plateau(NE-QTP)was an important corridor for early human occupation.We analyzed fossil fungal spore records for the last 8500 years from a high resolution aeolian section at Langgeri(LGR)on the NE-QTP.Thirty-two fungal spore types were identified in the LGR section,including seven coprophilous types.We combined analysis of coprophilous fungal spores,Cyperaceae,Artemisia,and Hippophae pollen,and the charcoal>50µm fraction to explore the timing and controls of pastoralism on the NE-QTP since the middle Holocene.Pastoralism commenced at LGR shortly before ca.5.5 ka and gradually increased between ca.5.5–3.5 ka,but markedly intensified after ca.2.2 ka,with three periods of growth at ca.2.1–1.9,1.4–1.2,and 0.6–0 ka,and a decline at ca.1.1–0.6 ka.The timing of changes in pastoral activity on the NE-QTP based on the coprophilous fungal spore record is supported by regional archeology and historical documents.Technological and political developments,rather than climate change,played key roles in the long-term fluctuations of regional pastoralism on the NE-QTP in the late Holocene.
基金This study was funded by the‘Science for a Sustainable Development’programme of the Belgian Federal Science Policy(project SD/BD/03 CLANIMAE)The fieldwork was conducted with permission of the Uganda National Council of Science and Technology(NS 162)and the Uganda Wildlife Authority(UWA/TBDP/RES/50).
文摘Mycological tools to estimate the effects of diverse land-use practices on fungal diversity are scarce,because of poor knowledge of the taxonomic diversity of tropical fungi and their response to anthropogenic habitat change.Here,we investigate assemblages of fungal spores,recently deposited in the bottom sediments of 24 small crater lakes in western Uganda,to assess the relationship between the local richness of fungi and environmental variation in the crater basin along regional gradients of natural vegetation and land use.We recovered~9500 fungal spore specimens,which could be attributed to 216 morphotypes.Using an information-theoretic approach based on the corrected Akaike Information Criterion(AICc),we determined the environmental factors which best explained variation in the diversity of fungal spores among three datasets:(i)the full set of 24 crater basins,(ii)the subset of 22 basins with freshwater lakes,and(iii)the subset of 17 basins partly or completely in agricultural use(cropland,fallow land,pasture and plantation).In these 17 human-impacted crater basins our results revealed a negative relationship between fungal spore richness and the areal fraction of basins in agricultural use.However,this detrimental effect of land use on fungal spore richness was not apparent across the full set of both disturbed and(presently)undisturbed basins.This was due to large variation in fungal spore richness among the undisturbed basins covered either with forest or savannah vegetation,probably resulting from site-specific controls on fungal habitat diversity,such as climatic moisture balance and the composition of natural and/or secondary vegetation.The land-use effects on fungal spore diversity,as documented in this study,suggest that communities of tropical fungi progressively exposed to land-use practices are threatened by species loss.Hence,our study demonstrates the need to develop conservation strategies mitigating the impacts of agriculture on the biodiversity of tropical fungi.
基金The research was funded by the Chinese Academy of Sciences[183611KYSB20200080]the National Natural Science Foundation of China[41871339,42071320,42071423,41801338]+2 种基金the National Special Support Program for High-level Personnel Recruitment(Wenjiang Huang)the Youth Innovation Promotion Association CAS(Huichun Ye)the Future Star Talent Program of Aerospace Information Research Institute,CAS(Huichun Ye).
文摘Yellow rust(Puccinia striiformis f.sp.Tritici)is a frequently occurring fungal disease of winter wheat(Triticum aestivum L.).During yellow rust infestation,fungal spores appear on the surface of the leaves as yellow and narrow stripes parallel to the leaf veins.We analyzed the effect of the fungal spores on the spectra of the diseased leaves to find a band sensitive to yellow rust and established a new vegetation index called the yellow rust spore index(YRSI).The estimation accuracy and stability were evaluated using two years of leaf spectral data,and the results were compared with eight indices commonly used for yellow rust detection.The results showed that the use of the YRSI ranked first for estimating the disease ratio for the 2017 spectral data(R^(2)=0.710,RMSE=0.097)and outperformed the published indices(R^(2)=0.587,RMSE=0.120)for the validation using the 2002 spectral data.The random forest(RF),k-nearest neighbor(KNN),and support vector machine(SVM)algorithms were used to test the discrimination ability of the YRSI and the eight commonly used indices using a mixed dataset of yellow-rust-infested,healthy,and aphid–infested wheat spectral data.The YRSI provided the best performance.
文摘Isolating cellulase-secreting microbes followed-by screening their cellulolytic activities has been an essential approach to discover novel and potential cellulases for cellulolytic industrial applications. This study was aimed to explore competitive exoglucanases by screening avicelase activities for 92 fungal strains isolated from environmental airborne-fungal-spore samples. Results showed that an isolated fungal strain numbered 58 exhibited the best avicelase activity of 0.209 U/mL when cultured for six days at pH 5.0 - 5.3 and 25℃ - 27℃, and was lately identified as a yeast strain of Meyerozyma sp. (96% ITS fragment similar with Meyerozyma caribbica, HG970748). Based on amino acid sequences revealed from LC/MS/MS, the target exoglucanase was identical to 1,4-beta-D-glucan cellobiohydrolases and was named Mc-CBHI which had optimal avicelase reaction conditions of pH 5 and 70℃ and could remain fairly stable after 4hr incubation at acid conditions (pH 3 - 5) or wide temperature ranges (30℃ - 80℃). Additionally, the Mc-CBHI (~70 kDa and ~3.6% of crude enzyme) had specific FPase and avicelase activities of 0.179 U/mg and 0.126 U/mg, respectively (which were about 40% - 50% activities of a commercial cellulase Accellerase-1000). These results demonstrated that the newly-found Mc-CBHI could become one of potential exoglucanase resources for related cellulolytic industrial applications.
文摘Recently, the pharmacological study of Ganoderma spores and active components has be-come a focus of attention in the world. The present reviewis based on the auctorial research on Ganoder-maspores. It involves pharmacological effects of Ganodermaspores and its active components, includingimmunomodulating effect, antitumor activity and its mechanismin vivoandin vitro, liver-protectiveeffect, gastric ulcer preventing effect, serum glucose and blood fat depressing effects, anti-hypoxia andscavenging free redical, etc. The possible problems and their solutions in this research area are also dis-cussed.
