Breeding of male-sterile lines has become the mainstream for the heterosis utilization in foxtail millet,but the genetic basis of most male-sterile lines used for the hybrid is still an area to be elucidated.In this s...Breeding of male-sterile lines has become the mainstream for the heterosis utilization in foxtail millet,but the genetic basis of most male-sterile lines used for the hybrid is still an area to be elucidated.In this study,a highly male-sterile line Gao146A was investigated.Genetic analysis indicated that the highly male-sterile phenotype was controlled by a single recessive gene a single recessive gene.Using F 2 population derived from cross Gao146A/K103,one gene controlling the highly male- sterility,tentatively named as ms1,which linked to SSR marker b234 with genetic distance of 16.7 cM,was mapped on the chromosome VI.These results not only laid the foundation for fine mapping of this highly male-sterile gene,but also helped to accelerate the improvement of highly male-sterile lines by using molecular marker assisted breeding method.展开更多
Arid and semi-arid regions of China account for more than half of the country. Because of drought resistance and high nutritive value, elite foxtail millet (Setaria Italica (L.) P. Beauv.) is one of the most important...Arid and semi-arid regions of China account for more than half of the country. Because of drought resistance and high nutritive value, elite foxtail millet (Setaria Italica (L.) P. Beauv.) is one of the most important cereal crops in China. Evaluation of germplasm and genetic diversity of foxtail millet is still in its infancy, but prolamin could play an important role as a protein marker. To investigate the genetic diversity and population structure of foxtail millet from different ecological zones of China, 90 accessions of foxtail millet were collected from three major ecological areas: North, Northwest, and Northeast China. The prolamin contents were examined by acid polyacrylamide gel electrophoresis (acid-PAGE). Five to twenty-two prolamin bands appeared in tested varieties, of which were polymorphic, so prolamin patterns of foxtail millet varieties can be used in variety identification and evaluation. Structure analysis identified six groups, which matches their pedigree information but not their geographic origins. This indicated a high degree (87.78%) of consistency with a phylogenetic classification based on SSR. The results showed prolamin banding patterns were an effective method for analyzing foxtail millet genetic variability.展开更多
Rice false smut is caused by ascomycete Villosiclava virens, whose potential alternative hosts have been assumed previ- ously. Here its potential alternative hosts were surveyed and identified from 2008 to 2013 in the...Rice false smut is caused by ascomycete Villosiclava virens, whose potential alternative hosts have been assumed previ- ously. Here its potential alternative hosts were surveyed and identified from 2008 to 2013 in the main rice-growing regions in China. Two common weeds in paddy fields, Digitaria sanguinalis Scop. and Echinochloa crusgalli （L.） Beauv., were found to present the similar symptoms to smut diseases in a few individuals in 2012 and 2013 in Zhejiang and Sichuan provinces of China, respectively. After the examinations of the spore morphology, their infection and extension mode in hosts, pathogen cell wall components, and molecular identification, the two pathogens were identified to be the Basidiomycetes, Ustilago syntherismae and Ustilago trichophora, respectively. So far there has been no alternative host of V. virens to be identified in China. These suggest that the alternative hosts of V. virens, if they do exist, are not possible to play an important role in the pathogen life cycle and the disease epidemics.展开更多
[ Objective] The paper was to optimize the optimal conditions for seed germination and seedling emergence of green foxtail [ Setar/a viridis （ L. ） Beauv. ], so as to solve the problem of serious damage caused by gr...[ Objective] The paper was to optimize the optimal conditions for seed germination and seedling emergence of green foxtail [ Setar/a viridis （ L. ） Beauv. ], so as to solve the problem of serious damage caused by green foxtail. [Method] Using the orthogenal test and the LSD method of software SPSS 18.0, with the number of emerged seedlings of green foxtail as the evaluation index, the influencing factors such as pretreatment of green foxtail, earth covering depth, soil temperature and soil moisture content were compared in the test. [Result] These four experimental factors had great impact on seed germination characteristics of green foxtail. The optimal scheme for seedling emergence of green foxtail was as follows： pretreatment of green foxtail for 3 d, earth covering depth 0.5 cm, soil temperature 32 ℃, soil moisture content 15%. The optimum condition was consistent with the actual situation of serious damage of green foxtail （ suffering moderate and heavy rainfall after sowing and before seedling of millet ）. Avoiding sowing millet before moderate and heavy rainfall could effectively control serious damage of green fox＇tail. [ Conclusion] The study provided theoretical support for effective control against the vicious weed green foxtail.展开更多
基金supported by the Postdoctoral Management Committee,China(92948)the Natural Science Foundation of Shanxi Province,China(2012011032-1)the Chinese Agricultural Research System(CARS-07)
文摘Breeding of male-sterile lines has become the mainstream for the heterosis utilization in foxtail millet,but the genetic basis of most male-sterile lines used for the hybrid is still an area to be elucidated.In this study,a highly male-sterile line Gao146A was investigated.Genetic analysis indicated that the highly male-sterile phenotype was controlled by a single recessive gene a single recessive gene.Using F 2 population derived from cross Gao146A/K103,one gene controlling the highly male- sterility,tentatively named as ms1,which linked to SSR marker b234 with genetic distance of 16.7 cM,was mapped on the chromosome VI.These results not only laid the foundation for fine mapping of this highly male-sterile gene,but also helped to accelerate the improvement of highly male-sterile lines by using molecular marker assisted breeding method.
文摘Arid and semi-arid regions of China account for more than half of the country. Because of drought resistance and high nutritive value, elite foxtail millet (Setaria Italica (L.) P. Beauv.) is one of the most important cereal crops in China. Evaluation of germplasm and genetic diversity of foxtail millet is still in its infancy, but prolamin could play an important role as a protein marker. To investigate the genetic diversity and population structure of foxtail millet from different ecological zones of China, 90 accessions of foxtail millet were collected from three major ecological areas: North, Northwest, and Northeast China. The prolamin contents were examined by acid polyacrylamide gel electrophoresis (acid-PAGE). Five to twenty-two prolamin bands appeared in tested varieties, of which were polymorphic, so prolamin patterns of foxtail millet varieties can be used in variety identification and evaluation. Structure analysis identified six groups, which matches their pedigree information but not their geographic origins. This indicated a high degree (87.78%) of consistency with a phylogenetic classification based on SSR. The results showed prolamin banding patterns were an effective method for analyzing foxtail millet genetic variability.
基金supported by the National Natural Science Foundation of China (31271999)the Special Fund for Agro-Scientific Research in the Public Interest, China (200903039-5)
文摘Rice false smut is caused by ascomycete Villosiclava virens, whose potential alternative hosts have been assumed previ- ously. Here its potential alternative hosts were surveyed and identified from 2008 to 2013 in the main rice-growing regions in China. Two common weeds in paddy fields, Digitaria sanguinalis Scop. and Echinochloa crusgalli （L.） Beauv., were found to present the similar symptoms to smut diseases in a few individuals in 2012 and 2013 in Zhejiang and Sichuan provinces of China, respectively. After the examinations of the spore morphology, their infection and extension mode in hosts, pathogen cell wall components, and molecular identification, the two pathogens were identified to be the Basidiomycetes, Ustilago syntherismae and Ustilago trichophora, respectively. So far there has been no alternative host of V. virens to be identified in China. These suggest that the alternative hosts of V. virens, if they do exist, are not possible to play an important role in the pathogen life cycle and the disease epidemics.
基金Supported by Science and Technology Support Program of Hebei Province(09250307D)Special Fund for Agro-scientific Research in the Public Interest(20120304201)
文摘[ Objective] The paper was to optimize the optimal conditions for seed germination and seedling emergence of green foxtail [ Setar/a viridis （ L. ） Beauv. ], so as to solve the problem of serious damage caused by green foxtail. [Method] Using the orthogenal test and the LSD method of software SPSS 18.0, with the number of emerged seedlings of green foxtail as the evaluation index, the influencing factors such as pretreatment of green foxtail, earth covering depth, soil temperature and soil moisture content were compared in the test. [Result] These four experimental factors had great impact on seed germination characteristics of green foxtail. The optimal scheme for seedling emergence of green foxtail was as follows： pretreatment of green foxtail for 3 d, earth covering depth 0.5 cm, soil temperature 32 ℃, soil moisture content 15%. The optimum condition was consistent with the actual situation of serious damage of green foxtail （ suffering moderate and heavy rainfall after sowing and before seedling of millet ）. Avoiding sowing millet before moderate and heavy rainfall could effectively control serious damage of green fox＇tail. [ Conclusion] The study provided theoretical support for effective control against the vicious weed green foxtail.
