The location of the toxin gene of B. thuringiensis subsp. galleriae (H5ab) on the Mr-130Mdplasmid is determined by molecular cloning. Double digestion fragments (BamHⅠ and SalⅠ)and PstⅠ restriction fragments as wel...The location of the toxin gene of B. thuringiensis subsp. galleriae (H5ab) on the Mr-130Mdplasmid is determined by molecular cloning. Double digestion fragments (BamHⅠ and SalⅠ)and PstⅠ restriction fragments as well, from the 130 Md plasmid of B. thuringiensis subsp.galleriae, are ligated with the cloning vector pAT 153 respectively and transformed into E.coli strain HB 101. Out of 208 transformants, three colonies (FG2, FG9, FG19) give posi-tive hybridization reaction using the HD-1 delta-endotoxin gene as a probe. They are presum-ed to contain the delta-endotoxin gene of B. thuringiensis subsp. galleriae. Western bolt assaysindicate that Mr-130 kDal and 68 kDal, crystal proteins produced by clone FG 2 react withanticrystal protein antibody. The protein extracts of clone FG2 are lethal to Ostrinia furna-calis (Guenee). This is the first report with regard to the cloning and expression of the B. thuringiensissubsp. galleriae (H5ab) delta-endotoxin gene.展开更多
The HeI photoelectron (PE) spectra of both 2(5H) furanone and its trans-chair-dimenc-compound (t-c-DFN) are reported.The assignment of the PES bands is made on the basis of band shapes,the PES results of the molecules...The HeI photoelectron (PE) spectra of both 2(5H) furanone and its trans-chair-dimenc-compound (t-c-DFN) are reported.The assignment of the PES bands is made on the basis of band shapes,the PES results of the molecules which have the similar atomic groups,and the restricted Hartree-Fock (RHF) calculations for the molecules studied.From the results of both PES experimental and theoretical calculations,it is proved that the ionization potential (IPs) of the HOMO for the dimenc-compound is lower than that of the HOMO for the monomer.And the total energy computed for the t-c-DFN is the lowest in the four possible configurations of dimeric-compounds of 2(5H) furanone Therefore the synthesis of t-c-DFN is also the easiest.展开更多
The stability constants of complex anion In(tiron)^-in mixed solvents of ethanol-water with weight percentages of ethanol being 0,5,15,25,35 and 50 have been measured spectrophotometrically at 293 K,using NaClO_4 as s...The stability constants of complex anion In(tiron)^-in mixed solvents of ethanol-water with weight percentages of ethanol being 0,5,15,25,35 and 50 have been measured spectrophotometrically at 293 K,using NaClO_4 as supporting electrolyte with ionic strength ranging from 0.1 to 3.3mol·dm^(-3). The thermodynamic stability constants of In(tiron)^-in various mixed solvents have been obtained by a polynomial approximation equation based on Pitzer's theory.As a comparison,the traditional Debye-Hückel equation has also been used to treat the same data.The results show that this polynomial approximate method is obviously superior to the old one.It was discovered that there is a linear rela- tion between the thermodynamic stability constants of In(tiron)^-and the mole fraction of organic component in mixed solvents or the reciprocal of dielectric constants of mixed solvents.The medium effect and the standard free energy of transfer,ΔG_(tr)~0,for the coordination reaction:In^(3+)+tiron^(4-)= In(tiron)^-,have also been discussed.展开更多
文摘The location of the toxin gene of B. thuringiensis subsp. galleriae (H5ab) on the Mr-130Mdplasmid is determined by molecular cloning. Double digestion fragments (BamHⅠ and SalⅠ)and PstⅠ restriction fragments as well, from the 130 Md plasmid of B. thuringiensis subsp.galleriae, are ligated with the cloning vector pAT 153 respectively and transformed into E.coli strain HB 101. Out of 208 transformants, three colonies (FG2, FG9, FG19) give posi-tive hybridization reaction using the HD-1 delta-endotoxin gene as a probe. They are presum-ed to contain the delta-endotoxin gene of B. thuringiensis subsp. galleriae. Western bolt assaysindicate that Mr-130 kDal and 68 kDal, crystal proteins produced by clone FG 2 react withanticrystal protein antibody. The protein extracts of clone FG2 are lethal to Ostrinia furna-calis (Guenee). This is the first report with regard to the cloning and expression of the B. thuringiensissubsp. galleriae (H5ab) delta-endotoxin gene.
基金Project supported by the National Natural Science Foundation of China
文摘The HeI photoelectron (PE) spectra of both 2(5H) furanone and its trans-chair-dimenc-compound (t-c-DFN) are reported.The assignment of the PES bands is made on the basis of band shapes,the PES results of the molecules which have the similar atomic groups,and the restricted Hartree-Fock (RHF) calculations for the molecules studied.From the results of both PES experimental and theoretical calculations,it is proved that the ionization potential (IPs) of the HOMO for the dimenc-compound is lower than that of the HOMO for the monomer.And the total energy computed for the t-c-DFN is the lowest in the four possible configurations of dimeric-compounds of 2(5H) furanone Therefore the synthesis of t-c-DFN is also the easiest.
基金the National Natural Science Foundation of China.
文摘The stability constants of complex anion In(tiron)^-in mixed solvents of ethanol-water with weight percentages of ethanol being 0,5,15,25,35 and 50 have been measured spectrophotometrically at 293 K,using NaClO_4 as supporting electrolyte with ionic strength ranging from 0.1 to 3.3mol·dm^(-3). The thermodynamic stability constants of In(tiron)^-in various mixed solvents have been obtained by a polynomial approximation equation based on Pitzer's theory.As a comparison,the traditional Debye-Hückel equation has also been used to treat the same data.The results show that this polynomial approximate method is obviously superior to the old one.It was discovered that there is a linear rela- tion between the thermodynamic stability constants of In(tiron)^-and the mole fraction of organic component in mixed solvents or the reciprocal of dielectric constants of mixed solvents.The medium effect and the standard free energy of transfer,ΔG_(tr)~0,for the coordination reaction:In^(3+)+tiron^(4-)= In(tiron)^-,have also been discussed.
