[Objectives]To explore the pharmacological effects of Gardenia jasminoides and its potential benefits on eye skin.[Methods]TCMSP and SymMap databases were used to screen the active components and corresponding targets...[Objectives]To explore the pharmacological effects of Gardenia jasminoides and its potential benefits on eye skin.[Methods]TCMSP and SymMap databases were used to screen the active components and corresponding targets of G.jasminoides.Human eye skin-related targets were screened,and the active component-target network and protein-protein interaction(PPI)network were established.Gene ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis were performed.[Results]Twenty-six active compounds were screened out from G.jasminoides,and 277 targets were obtained.From the Gencards database,26652 disease targets were retrieved and 205 related gene targets were screened.The active component-action target network of G.jasminoides constructed by Cytoscape software revealed the potential of G.jasminoides to play a role in multiple biological pathways.In addition,PPI-network analysis,GO function analysis and KEGG pathway enrichment analysis revealed that the active components of G.jasminoides mainly regulate the biological processes such as inflammatory response,oxidative stress and apoptosis,involving MAPK,NF-κB and other important signaling pathways.[Conclusions]This study provides a theoretical basis for the eye skin protection of G.jasminoides and an important clue for future drug development.展开更多
[Objectives]This study was conducted to investigate the response of Gardenia to purple soil drought stress,hoping to provide a reference for the selection of plants for vegetation restoration in purple soil regions.[M...[Objectives]This study was conducted to investigate the response of Gardenia to purple soil drought stress,hoping to provide a reference for the selection of plants for vegetation restoration in purple soil regions.[Methods]The pot-weighing water control method was used to apply different degrees of drought stress to Gardenia seedlings in purple soil,and the effects of drought stress on the electrical conductivity,chlorophyll content,leaf morphology and structure of Gardenia leaves were explored.[Results]The leaf electrical conductivity increased with the increase of drought stress intensity,and the leaf electrical conductivity under severe drought stress increased by 59.93%compared with the control;the chlorophyll content of Gardenia showed a single-peak changing trend that increased and then decreased with the development of drought stress,and it was the highest in each stress stage under severe drought stress;the leaf thickness,palisade tissue thickness and sponge tissue thickness of Gardenia were reduced with the stress degree increasing,and showed the largest decreases under severe stress;the stomatal length,stomatal width and stomatal opening of Gardenia gradually decreased with the increase of stress,while the stomatal density gradually increased.[Conclusions]This study provides a technical and resource basis for vegetation restoration in purple soil.展开更多
[Objectives]This study was conducted to improve the propagation efficiency of Gardenia jasminoides Ellis and improve the quality of plantlets.[Methods]A G.jasminoides cutting experiment was carried out with three diff...[Objectives]This study was conducted to improve the propagation efficiency of Gardenia jasminoides Ellis and improve the quality of plantlets.[Methods]A G.jasminoides cutting experiment was carried out with three different cuttage modes and two cutting treatment methods,and the rooting time,rooting rate,root amount and plantlet growth were measured and analyzed.[Results]The three-dimensional cutting model of CL1 could not only shorten the nursery cycle and promote root development,but also effectively improve the quality of plantlets and increase the yield of plantlets per unit area.It is an ideal way to improve the quality and efficiency of cutting plantlets.[Conclusions]This study has a positive role in promoting the healthy development of the nursery stock industry.展开更多
In order to protect and develop the local resources of Gardenia jasminoides Ellis with Hunan characteristics,this paper uses the existing technology,analyzes the existing achievements in the cultivation of G.jasminoid...In order to protect and develop the local resources of Gardenia jasminoides Ellis with Hunan characteristics,this paper uses the existing technology,analyzes the existing achievements in the cultivation of G.jasminoides Ellis,investigates and analyzes the actual situation of the seedling base and cooperatives in Hunan,and collects the relevant index data related to the cultivation of G.jasminoides Ellis.This paper summarizes the practical techniques related to land selection,soil preparation,seedling breeding,afforestation technology,tending management,pest control and so on,in order to provide reference basis for planting and cultivateon and industrial development of G.jasminoides Ellis in Hunan.展开更多
A new iridoid glycoside, 6＇-O-sinapoylgeniposide, was isolated from Gardeniajasminoides Ellis and its structure was elucidated on the basis of 1D and 2D NMR, HR-ESI-MS techniques.
AIM： To investigate the effect of Garden/a fasm/noides （GJ） on cerulein-induced acute pancreatitis （AP） in mice. METHODS： C57BL/6 mice weighing 18-20 g were divided into three groups. （1） Normal salinetreated ...AIM： To investigate the effect of Garden/a fasm/noides （GJ） on cerulein-induced acute pancreatitis （AP） in mice. METHODS： C57BL/6 mice weighing 18-20 g were divided into three groups. （1） Normal salinetreated group, （2） treatment with GJ at a dose of 0.1 g/kg, （3） treatment with GJ at a dose of 1 g/kg. GJ was administered orally （n = 6 per group） for 1 wk. Three hours later, the mice were given anintraperitoneal injection of cerulein （50 μg/kg）, a stable cholecystokinin （CCK） analogue, every hour for a total of 6h as described previously. The mice were sacrificed at 6 h after completion of cerulein injections. Blood samples were obtained to determine serum amylase, lipase and cytokine levels. The pancreas was rapidly removed for morphologic examination and scoring. A portion of pancreas was stored at -70℃ and prepared for the measurement of tissue myeloperoxidase （MPO） activity, an indicator of neutrophil sequestration, and for reverse-transcriptase PCR （RT-PCR） and real-time PCR measurements. RESULTS： Treatment with G.1 decreased significantly the severity of pancreatitis and pancreatitis-associated lung injury. Treatment with G.1 attenuated the severity of AP compared with saline-treated mice, as shown by reduction in pancreatic edema, neutrophil infiltration, serum amylase and lipase levels, serum cytokine levels, and mRNA expression of multiple inflammatory mediators. CONCLUSION： These results suggest that GJ attenuated the severity of AP as well as pancreatitisassociated lung injury.展开更多
AIM:To investigate the anti-hepatofibrotic effects of Gardenia jasminoides in liver fibrosis.METHODS:Male Sprague-Dawley rats underwent common bile duct ligation(BDL) for 14 d and were treated with Gardenia jasminoide...AIM:To investigate the anti-hepatofibrotic effects of Gardenia jasminoides in liver fibrosis.METHODS:Male Sprague-Dawley rats underwent common bile duct ligation(BDL) for 14 d and were treated with Gardenia jasminoides by gavage.The ef-fects of Gardenia jasminoides on liver fibrosis and the detailed molecular mechanisms were also assessed in human hepatic stellate cells(LX-2) in vitro.RESULTS:Treatment with Gardenia jasminoides decreased serum alanine aminotransferase(BDL vs BDL + 100 mg/kg Gardenia jasminoides,146.6 ± 15 U/L vs 77 ± 6.5 U/L,P = 0.0007) and aspartate aminotransferase(BDL vs BDL + 100 mg/kg Gardenia jasminoides,188 ± 35.2 U/L vs 128 ± 19 U/L,P = 0.005) as well as hydroxyproline(BDL vs BDL + 100 mg/kg Gardenia jasminoides,438 ± 40.2 μg/g vs 228 ± 10.3 μg/g liver tissue,P = 0.004) after BDL.Furthermore,Gardenia jasminoides significantly reduced liver mRNA and/or protein expression of transforming growth factor β1(TGF-β1),collagen type?Ⅰ?(Col?Ⅰ) and α-smooth muscle actin(α-SMA).Gardenia jasminoides significantly suppressed the upregulation of TGF-β1,Col?Ⅰand α-SMA in LX-2 exposed to recombinant TGF-β1.Moreover,Gardenia jasminoides inhibited TGF-β1-induced Smad2 phosphorylation in LX-2 cells.CONCLUSION:Gardenia jasminoides exerts antifibrotic effects in the liver fibrosis and may represent a novel antifibrotic agent.展开更多
Objectives： Gardenia Jasminoides Ellis（Zhizi）, belonging to Rubiaceae family, has been traditionally used for treatment cholestasis and jaundice for centuries in Asian countries. In the theory of Traditional Chines...Objectives： Gardenia Jasminoides Ellis（Zhizi）, belonging to Rubiaceae family, has been traditionally used for treatment cholestasis and jaundice for centuries in Asian countries. In the theory of Traditional Chinese Medicines, Zhizi could dispel dampness and heat via the urine to execute its choleretic effects. However, the potential molecular mechanism has been still poorly clarified. Here, we investigated the effect of different dose of Zhizi aqueous extract powder（0.3 g/kg/d and 0.9 g/kg/d） on urinary excretion of bile acids（BAs）, and defined the potential mechanism via renal BAs efflux transporters Mrp2 and Mrp4 in normal rats.Methods： Male Wistar rats were orally administrated with 0.3 or 0.9 g/kg/d dose of Zhizi aqueous extract powder for 2 weeks, then body weight, serum aminotransferase, total BAs concentrations in liver, bile,serum, kidney and urine, 1 h bile flow, 12-h urinary volume, biliary and urinary excretion amount of total BAs as well as protein expression of major renal BAs efflux transporter Mrp2 and Mrp4, were all evaluated.Results： Zhizi especially the high dose of Zhizi aqueous extract powder could reduce hepatic total BAs concentration. Additionally, bile flow and biliary excretion had no significant difference, but the remarkable increasing urinary excretion of BAs and 2 to 3 folds up-regulated renal Mrp2 expression were observed after administrated with Zhizi as compared with the control group.Conclusion： The findings indicate that Zhizi reduces hepatic total BAs level by increasing urinary excretion rather than the biliary excretion of BAs, which, in turn ascribed to elevated protein expression of Mrp2 at apical membrane of renal tubular epithelial cells.展开更多
文摘[Objectives]To explore the pharmacological effects of Gardenia jasminoides and its potential benefits on eye skin.[Methods]TCMSP and SymMap databases were used to screen the active components and corresponding targets of G.jasminoides.Human eye skin-related targets were screened,and the active component-target network and protein-protein interaction(PPI)network were established.Gene ontology(GO)analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis were performed.[Results]Twenty-six active compounds were screened out from G.jasminoides,and 277 targets were obtained.From the Gencards database,26652 disease targets were retrieved and 205 related gene targets were screened.The active component-action target network of G.jasminoides constructed by Cytoscape software revealed the potential of G.jasminoides to play a role in multiple biological pathways.In addition,PPI-network analysis,GO function analysis and KEGG pathway enrichment analysis revealed that the active components of G.jasminoides mainly regulate the biological processes such as inflammatory response,oxidative stress and apoptosis,involving MAPK,NF-κB and other important signaling pathways.[Conclusions]This study provides a theoretical basis for the eye skin protection of G.jasminoides and an important clue for future drug development.
基金Hunan Forestry Science and Technology Innovation Project(XLK201971)Changsha Science and Technology Program(kq1801028).
文摘[Objectives]This study was conducted to investigate the response of Gardenia to purple soil drought stress,hoping to provide a reference for the selection of plants for vegetation restoration in purple soil regions.[Methods]The pot-weighing water control method was used to apply different degrees of drought stress to Gardenia seedlings in purple soil,and the effects of drought stress on the electrical conductivity,chlorophyll content,leaf morphology and structure of Gardenia leaves were explored.[Results]The leaf electrical conductivity increased with the increase of drought stress intensity,and the leaf electrical conductivity under severe drought stress increased by 59.93%compared with the control;the chlorophyll content of Gardenia showed a single-peak changing trend that increased and then decreased with the development of drought stress,and it was the highest in each stress stage under severe drought stress;the leaf thickness,palisade tissue thickness and sponge tissue thickness of Gardenia were reduced with the stress degree increasing,and showed the largest decreases under severe stress;the stomatal length,stomatal width and stomatal opening of Gardenia gradually decreased with the increase of stress,while the stomatal density gradually increased.[Conclusions]This study provides a technical and resource basis for vegetation restoration in purple soil.
基金Supported by Changsha Science and Technology Planning Project (kq1801028)Major Landmark Innovation Demonstration Project (2019XK2002)
文摘[Objectives]This study was conducted to improve the propagation efficiency of Gardenia jasminoides Ellis and improve the quality of plantlets.[Methods]A G.jasminoides cutting experiment was carried out with three different cuttage modes and two cutting treatment methods,and the rooting time,rooting rate,root amount and plantlet growth were measured and analyzed.[Results]The three-dimensional cutting model of CL1 could not only shorten the nursery cycle and promote root development,but also effectively improve the quality of plantlets and increase the yield of plantlets per unit area.It is an ideal way to improve the quality and efficiency of cutting plantlets.[Conclusions]This study has a positive role in promoting the healthy development of the nursery stock industry.
基金Supported by Changsha Science and Technology Project(kq1801028)Major Landmark Innovation Demonstration Project(2019XK2002).
文摘In order to protect and develop the local resources of Gardenia jasminoides Ellis with Hunan characteristics,this paper uses the existing technology,analyzes the existing achievements in the cultivation of G.jasminoides Ellis,investigates and analyzes the actual situation of the seedling base and cooperatives in Hunan,and collects the relevant index data related to the cultivation of G.jasminoides Ellis.This paper summarizes the practical techniques related to land selection,soil preparation,seedling breeding,afforestation technology,tending management,pest control and so on,in order to provide reference basis for planting and cultivateon and industrial development of G.jasminoides Ellis in Hunan.
基金supported by the National Natural Science Foundation of China(No.30600805)supported by Program for New Century Excellent Talents in University(No.NCET-06-0497).
文摘A new iridoid glycoside, 6＇-O-sinapoylgeniposide, was isolated from Gardeniajasminoides Ellis and its structure was elucidated on the basis of 1D and 2D NMR, HR-ESI-MS techniques.
基金The Ministry of Science & Technology (MoST)/Korea Science & Engineering Foundation (KOSEF) through the Vestibulocochlear Research Center (VCRC) at Wonkwang University (R13-2002-055-00000-0)
文摘AIM： To investigate the effect of Garden/a fasm/noides （GJ） on cerulein-induced acute pancreatitis （AP） in mice. METHODS： C57BL/6 mice weighing 18-20 g were divided into three groups. （1） Normal salinetreated group, （2） treatment with GJ at a dose of 0.1 g/kg, （3） treatment with GJ at a dose of 1 g/kg. GJ was administered orally （n = 6 per group） for 1 wk. Three hours later, the mice were given anintraperitoneal injection of cerulein （50 μg/kg）, a stable cholecystokinin （CCK） analogue, every hour for a total of 6h as described previously. The mice were sacrificed at 6 h after completion of cerulein injections. Blood samples were obtained to determine serum amylase, lipase and cytokine levels. The pancreas was rapidly removed for morphologic examination and scoring. A portion of pancreas was stored at -70℃ and prepared for the measurement of tissue myeloperoxidase （MPO） activity, an indicator of neutrophil sequestration, and for reverse-transcriptase PCR （RT-PCR） and real-time PCR measurements. RESULTS： Treatment with G.1 decreased significantly the severity of pancreatitis and pancreatitis-associated lung injury. Treatment with G.1 attenuated the severity of AP compared with saline-treated mice, as shown by reduction in pancreatic edema, neutrophil infiltration, serum amylase and lipase levels, serum cytokine levels, and mRNA expression of multiple inflammatory mediators. CONCLUSION： These results suggest that GJ attenuated the severity of AP as well as pancreatitisassociated lung injury.
基金Supported by The Natural Science Foundation of China,No.81170450 to Lu MQ and No.81200308 to Lan TThe PhD Start-up Fund of Natural Science Foundation of Guangdong Province,China,No.S2012040008026The New Star of Science and Technology Foundation of Zhu Jiang in Guangzhou City
文摘AIM:To investigate the anti-hepatofibrotic effects of Gardenia jasminoides in liver fibrosis.METHODS:Male Sprague-Dawley rats underwent common bile duct ligation(BDL) for 14 d and were treated with Gardenia jasminoides by gavage.The ef-fects of Gardenia jasminoides on liver fibrosis and the detailed molecular mechanisms were also assessed in human hepatic stellate cells(LX-2) in vitro.RESULTS:Treatment with Gardenia jasminoides decreased serum alanine aminotransferase(BDL vs BDL + 100 mg/kg Gardenia jasminoides,146.6 ± 15 U/L vs 77 ± 6.5 U/L,P = 0.0007) and aspartate aminotransferase(BDL vs BDL + 100 mg/kg Gardenia jasminoides,188 ± 35.2 U/L vs 128 ± 19 U/L,P = 0.005) as well as hydroxyproline(BDL vs BDL + 100 mg/kg Gardenia jasminoides,438 ± 40.2 μg/g vs 228 ± 10.3 μg/g liver tissue,P = 0.004) after BDL.Furthermore,Gardenia jasminoides significantly reduced liver mRNA and/or protein expression of transforming growth factor β1(TGF-β1),collagen type?Ⅰ?(Col?Ⅰ) and α-smooth muscle actin(α-SMA).Gardenia jasminoides significantly suppressed the upregulation of TGF-β1,Col?Ⅰand α-SMA in LX-2 exposed to recombinant TGF-β1.Moreover,Gardenia jasminoides inhibited TGF-β1-induced Smad2 phosphorylation in LX-2 cells.CONCLUSION:Gardenia jasminoides exerts antifibrotic effects in the liver fibrosis and may represent a novel antifibrotic agent.
基金supported by the Prof. Xinan Wu’s,Dr. Yuhui Wei’s and Mr Guoqiang Zhang’s National Natural Science Foundation of China (Number:81373494,81373927,81641142 and 81702853)
文摘Objectives： Gardenia Jasminoides Ellis（Zhizi）, belonging to Rubiaceae family, has been traditionally used for treatment cholestasis and jaundice for centuries in Asian countries. In the theory of Traditional Chinese Medicines, Zhizi could dispel dampness and heat via the urine to execute its choleretic effects. However, the potential molecular mechanism has been still poorly clarified. Here, we investigated the effect of different dose of Zhizi aqueous extract powder（0.3 g/kg/d and 0.9 g/kg/d） on urinary excretion of bile acids（BAs）, and defined the potential mechanism via renal BAs efflux transporters Mrp2 and Mrp4 in normal rats.Methods： Male Wistar rats were orally administrated with 0.3 or 0.9 g/kg/d dose of Zhizi aqueous extract powder for 2 weeks, then body weight, serum aminotransferase, total BAs concentrations in liver, bile,serum, kidney and urine, 1 h bile flow, 12-h urinary volume, biliary and urinary excretion amount of total BAs as well as protein expression of major renal BAs efflux transporter Mrp2 and Mrp4, were all evaluated.Results： Zhizi especially the high dose of Zhizi aqueous extract powder could reduce hepatic total BAs concentration. Additionally, bile flow and biliary excretion had no significant difference, but the remarkable increasing urinary excretion of BAs and 2 to 3 folds up-regulated renal Mrp2 expression were observed after administrated with Zhizi as compared with the control group.Conclusion： The findings indicate that Zhizi reduces hepatic total BAs level by increasing urinary excretion rather than the biliary excretion of BAs, which, in turn ascribed to elevated protein expression of Mrp2 at apical membrane of renal tubular epithelial cells.
