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Study on prebiotic effectiveness of neutral garlic fructan in vitro
被引量:
1
1
作者
Ning Zhang
Xuesong Huang
+2 位作者
Yanhua Zeng
Xiyang Wu
Xichun Peng
《Food Science and Human Wellness》
SCIE
2013年第3期119-123,共5页
Garlic is traditionally believed to have many health benefits including prevention of gastrointestinal diseases.One of the major components,garlic fructan(GF),was evaluated for its prebiotic effectiveness on human int...
Garlic is traditionally believed to have many health benefits including prevention of gastrointestinal diseases.One of the major components,garlic fructan(GF),was evaluated for its prebiotic effectiveness on human intestinal microflora.Garlic fructans A(DP 16)and B(DP 21)were prepared by ethanol fractionation precipitation.Then,they were added to an in vitro fermentation system as the sole carbon source inoculated with human fecal suspension.The total anaerobic bacteria,Bacteroides and Bifidobacteria,were enumerated by plate counting on selective media.Terminal restriction fragment length polymerization(tRFLP)was used to analyze DNA extracted from the in vitro cultures.The results indicated that the log CFUs of both Bacteroides(GF A 6.96,GF B 7.15)and Bifidobacteria(GF A 7.74,GF B 7.74)grown in the GF cultures at 24 h were significantly higher than those at 0 h(Bacteroides 4.93,Bifidobacteria 4.78)(P<0.05).Terminal restriction fragments(TRFs)256–258 bp(ascribed to Bifidobacterium)in the profiles were also observed higher in the TRFLP profiles from the garlic fructans’cultures.In this study,GFs were found to selectively stimulate the growth of beneficial Bifidobacteria from human fecal microflora.The prebiotic effectiveness of GFs supports the use of garlic as a way to prevent some gastrointestinal diseases.©2013 Beijing Academy of Food Sciences.Production and hosting by Elsevier B.V.All rights reserved.
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关键词
garlic
fructans
Prebiotic
effectiveness
In
vitro
fermentation
Fecal
bacteria
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题名
Study on prebiotic effectiveness of neutral garlic fructan in vitro
被引量:
1
1
作者
Ning Zhang
Xuesong Huang
Yanhua Zeng
Xiyang Wu
Xichun Peng
机构
Department of Food Science and Engineering
出处
《Food Science and Human Wellness》
SCIE
2013年第3期119-123,共5页
基金
We are grateful to the National Natural Science Foundation of China for financial support(No.31171722).
文摘
Garlic is traditionally believed to have many health benefits including prevention of gastrointestinal diseases.One of the major components,garlic fructan(GF),was evaluated for its prebiotic effectiveness on human intestinal microflora.Garlic fructans A(DP 16)and B(DP 21)were prepared by ethanol fractionation precipitation.Then,they were added to an in vitro fermentation system as the sole carbon source inoculated with human fecal suspension.The total anaerobic bacteria,Bacteroides and Bifidobacteria,were enumerated by plate counting on selective media.Terminal restriction fragment length polymerization(tRFLP)was used to analyze DNA extracted from the in vitro cultures.The results indicated that the log CFUs of both Bacteroides(GF A 6.96,GF B 7.15)and Bifidobacteria(GF A 7.74,GF B 7.74)grown in the GF cultures at 24 h were significantly higher than those at 0 h(Bacteroides 4.93,Bifidobacteria 4.78)(P<0.05).Terminal restriction fragments(TRFs)256–258 bp(ascribed to Bifidobacterium)in the profiles were also observed higher in the TRFLP profiles from the garlic fructans’cultures.In this study,GFs were found to selectively stimulate the growth of beneficial Bifidobacteria from human fecal microflora.The prebiotic effectiveness of GFs supports the use of garlic as a way to prevent some gastrointestinal diseases.©2013 Beijing Academy of Food Sciences.Production and hosting by Elsevier B.V.All rights reserved.
关键词
garlic
fructans
Prebiotic
effectiveness
In
vitro
fermentation
Fecal
bacteria
分类号
TS255.1 [轻工技术与工程—农产品加工及贮藏工程]
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Study on prebiotic effectiveness of neutral garlic fructan in vitro
Ning Zhang
Xuesong Huang
Yanhua Zeng
Xiyang Wu
Xichun Peng
《Food Science and Human Wellness》
SCIE
2013
1
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