[Objective] The growth inhibitory effects of garlic polysaccharide(GPS) on human Hep G2 cells were evaluated in this paper. [Method] Hep G2 cells were treated with GPS for 48 h for morphology assay by transition elect...[Objective] The growth inhibitory effects of garlic polysaccharide(GPS) on human Hep G2 cells were evaluated in this paper. [Method] Hep G2 cells were treated with GPS for 48 h for morphology assay by transition electron microscope. Anti-proliferative effects with the same treatment for 24 hand 48 h were assayed by MTT method.Cell cycle distribution and apoptosis assay of treated cells were performed in flow cytometry. [Result] The results showed that GPS enhanced growth inhibitory effect on Hep G2 cells in a time-and dose-dependent manner. PI(Propidium iodide)/Annexin V staining analyzed by FCM(flow cytometry) demonstrated that GPS has a cytotoxic effect on tumor cells. Cell cycle arrest of Hep G2 treated with GPS occurred in G2 phase. [Conclusion] This study suggests that GPS could exert an antitumor effect and could be used as a therapeutic agent for live cancer.展开更多
In the present work,garlic polysaccharide(GP)in lowering lipid based on in vitro and in vivo approaches were investigated.The in vitro studies revealed significant effects of GP on cholesterol,sodium cholate,oil bindi...In the present work,garlic polysaccharide(GP)in lowering lipid based on in vitro and in vivo approaches were investigated.The in vitro studies revealed significant effects of GP on cholesterol,sodium cholate,oil binding,and lipase inhibition.Meanwhile,the liver index in the medium and high dose GP group was significantly lower than the control group in normal mice which relatively corresponded to the modulated effect of intestinal flora.A high dose of GP has markedly reduced the ratio of intestinal Firmicutes to Bacteroidetes and increased the abundance of gut probiotics Akkermansia.Furthermore,the high dose of GP also significantly reduced the levels of triglyceride and low-density lipoprotein cholesterol of diabetic mice indicating that GP could not only prevent hyperlipidemia of metabolic syndrome but also further ameliorate dyslipidemia of diabetes mellitus.This study provides new prospects for garlic polysaccharide as a hypolipidemic nutraceutical for the treatment of metabolic syndrome and type 2 diabetes.展开更多
基金Supported by Science and Technology Bureau of Zhengzhou City(141PGJHZ541)
文摘[Objective] The growth inhibitory effects of garlic polysaccharide(GPS) on human Hep G2 cells were evaluated in this paper. [Method] Hep G2 cells were treated with GPS for 48 h for morphology assay by transition electron microscope. Anti-proliferative effects with the same treatment for 24 hand 48 h were assayed by MTT method.Cell cycle distribution and apoptosis assay of treated cells were performed in flow cytometry. [Result] The results showed that GPS enhanced growth inhibitory effect on Hep G2 cells in a time-and dose-dependent manner. PI(Propidium iodide)/Annexin V staining analyzed by FCM(flow cytometry) demonstrated that GPS has a cytotoxic effect on tumor cells. Cell cycle arrest of Hep G2 treated with GPS occurred in G2 phase. [Conclusion] This study suggests that GPS could exert an antitumor effect and could be used as a therapeutic agent for live cancer.
基金financially supported by the Key Laboratory of Comprehensive Utilization of Plant Resources in Hebei Province through the Fundamental Research Grant Scheme(215A2801D).
文摘In the present work,garlic polysaccharide(GP)in lowering lipid based on in vitro and in vivo approaches were investigated.The in vitro studies revealed significant effects of GP on cholesterol,sodium cholate,oil binding,and lipase inhibition.Meanwhile,the liver index in the medium and high dose GP group was significantly lower than the control group in normal mice which relatively corresponded to the modulated effect of intestinal flora.A high dose of GP has markedly reduced the ratio of intestinal Firmicutes to Bacteroidetes and increased the abundance of gut probiotics Akkermansia.Furthermore,the high dose of GP also significantly reduced the levels of triglyceride and low-density lipoprotein cholesterol of diabetic mice indicating that GP could not only prevent hyperlipidemia of metabolic syndrome but also further ameliorate dyslipidemia of diabetes mellitus.This study provides new prospects for garlic polysaccharide as a hypolipidemic nutraceutical for the treatment of metabolic syndrome and type 2 diabetes.
