Oxygen tension modulates cell function in an in vitro three-dimensional glioblastoma tumor model

Hypoxia is a typical feature of the tumor microenvironment,one of the most critical factors affecting cell behavior and tumor progression.However,the lack of tumor models able to precisely emulate natural brain tumor tissue has impeded the study of the effects of hypoxia on the progression and growth of tumor cells.This study reports a three-dimensional(3D)brain tumor model obtained by encapsulating U87MG(U87)cells in a hydrogel containing type I collagen.It also documents the effect of various oxygen concentrations(1%,7%,and 21%)in the culture environment on U87 cell morphology,proliferation,viability,cell cycle,apoptosis rate,and migration.Finally,it compares two-dimensional(2D)and 3D cultures.For comparison purposes,cells cultured in flat culture dishes were used as the control(2D model).Cells cultured in the 3D model proliferated more slowly but had a higher apoptosis rate and proportion of cells in the resting phase(G0 phase)/gap I phase(G1 phase)than those cultured in the 2D model.Besides,the two models yielded significantly different cell morphologies.Finally,hypoxia(e.g.,1%O2)affected cell morphology,slowed cell growth,reduced cell viability,and increased the apoptosis rate in the 3D model.These results indicate that the constructed 3D model is effective for investigating the effects of biological and chemical factors on cell morphology and function,and can be more representative of the tumor microenvironment than 2D culture systems.The developed 3D glioblastoma tumor model is equally applicable to other studies in pharmacology and pathology.

Three-dimensional structural models,evolution and petroleum geological significances of transtensional faults in the Ziyang area,central Sichuan Basin,SW China

With drilling and seismic data of Transtensional(strike-slip)Fault System in the Ziyang area of the central Sichuan Basin,SW China plane-section integrated structural interpretation,3-D fault framework model building,fault throw analyzing,and balanced profile restoration,it is pointed out that the transtensional fault system in the Ziyang 3-D seismic survey consists of the northeast-trending F_(I)19 and F_(I)20 fault zones dominated by extensional deformation,as well as 3 sets of northwest-trending en echelon normal faults experienced dextral shear deformation.Among them,the F_(I)19 and F_(I)20 fault zones cut through the Neoproterozoic to Lower Triassic Jialingjiang Formation,presenting a 3-D structure of an“S”-shaped ribbon.And before Permian and during the Early Triassic,the F_(I)19 and F_(I)20 fault zones underwent at least two periods of structural superimposition.Besides,the 3 sets of northwest-trending en echelon normal faults are composed of small normal faults arranged in pairs,with opposite dip directions and partially left-stepped arrangement.And before Permian,they had formed almost,restricting the eastward growth and propagation of the F_(I)19 fault zone.The F_(I)19 and F_(I)20 fault zones communicate multiple sets of source rocks and reservoirs from deep to shallow,and the timing of fault activity matches well with oil and gas generation peaks.If there were favorable Cambrian-Triassic sedimentary facies and reservoirs developing on the local anticlinal belts of both sides of the F_(I)19 and F_(I)20 fault zones,the major reservoirs in this area are expected to achieve breakthroughs in oil and gas exploration.

Three-Dimensional Analytical Modeling of Axial-Flux Permanent Magnet Drivers

In this paper, the axial-flux permanent magnet driver is modeledand analyzed in a simple and novel way under three-dimensional cylindricalcoordinates. The inherent three-dimensional characteristics of the deviceare comprehensively considered, and the governing equations are solved bysimplifying the boundary conditions. The axial magnetization of the sectorshapedpermanent magnets is accurately described in an algebraic form bythe parameters, which makes the physical meaning more explicit than thepurely mathematical expression in general series forms. The parameters of theBessel function are determined simply and the magnetic field distribution ofpermanent magnets and the air-gap is solved. Furthermore, the field solutionsare completely analytical, which provides convenience and satisfactoryaccuracy for modeling a series of electromagnetic performance parameters,such as the axial electromagnetic force density, axial electromagnetic force,and electromagnetic torque. The correctness and accuracy of the analyticalmodels are fully verified by three-dimensional finite element simulations and a15 kW prototype and the results of calculations, simulations, and experimentsunder three methods are highly consistent. The influence of several designparameters on magnetic field distribution and performance is studied and discussed.The results indicate that the modeling method proposed in this papercan calculate the magnetic field distribution and performance accurately andrapidly, which affords an important reference for the design and optimizationof axial-flux permanent magnet drivers.

Discontinuity development patterns and the challenges for 3D discrete fracture network modeling on complicated exposed rock surfaces

Natural slopes usually display complicated exposed rock surfaces that are characterized by complex and substantial terrain undulation and ubiquitous undesirable phenomena such as vegetation cover and rockfalls.This study presents a systematic outcrop research of fracture pattern variations in a complicated rock slope,and the qualitative and quantitative study of the complex phenomena impact on threedimensional(3D)discrete fracture network(DFN)modeling.As the studies of the outcrop fracture pattern have been so far focused on local variations,thus,we put forward a statistical analysis of global variations.The entire outcrop is partitioned into several subzones,and the subzone-scale variability of fracture geometric properties is analyzed(including the orientation,the density,and the trace length).The results reveal significant variations in fracture characteristics(such as the concentrative degree,the average orientation,the density,and the trace length)among different subzones.Moreover,the density of fracture sets,which is approximately parallel to the slope surface,exhibits a notably higher value compared to other fracture sets across all subzones.To improve the accuracy of the DFN modeling,the effects of three common phenomena resulting from vegetation and rockfalls are qualitatively analyzed and the corresponding quantitative data processing solutions are proposed.Subsequently,the 3D fracture geometric parameters are determined for different areas of the high-steep rock slope in terms of the subzone dimensions.The results show significant variations in the same set of 3D fracture parameters across different regions with density differing by up to tenfold and mean trace length exhibiting differences of 3e4 times.The study results present precise geological structural information,improve modeling accuracy,and provide practical solutions for addressing complex outcrop issues.

Implementation of a particle-in-cell method for the energy solver in 3D spherical geodynamic modeling

The thermal evolution of the Earth’s interior and its dynamic effects are the focus of Earth sciences.However,the commonly adopted grid-based temperature solver is usually prone to numerical oscillations,especially in the presence of sharp thermal gradients,such as when modeling subducting slabs and rising plumes.This phenomenon prohibits the correct representation of thermal evolution and may cause incorrect implications of geodynamic processes.After examining several approaches for removing these numerical oscillations,we show that the Lagrangian method provides an ideal way to solve this problem.In this study,we propose a particle-in-cell method as a strategy for improving the solution to the energy equation and demonstrate its effectiveness in both one-dimensional and three-dimensional thermal problems,as well as in a global spherical simulation with data assimilation.We have implemented this method in the open-source finite-element code CitcomS,which features a spherical coordinate system,distributed memory parallel computing,and data assimilation algorithms.

Research on Three-Dimensional Simulation of the Internal Arc Gear Skiving

Aiming at the problems that the simulation accuracy which is reduced due to the simplification of the model,a three-dimensional simulation method based on solid modeling is being proposed.By analyzing the motion relationship and positional relationship between the caries knife and the workpiece,the coordinate system of the caries machining was established.With the MATLAB software,the cutting edge model and the blade sweeping surface model of the boring cutter are sequentially established.Boolean operation is performed on the blade swept surface formed by the tooth cutter teeth with time t and the workpiece tooth geometry as well as the undeformed three-dimensional chip geometry model and the instantaneous cogging geometry model are obtained at different times.Through the compare between gear end face simulation tooth profile and the theoretical inner arc tooth profile,we verified the accuracy and rationality of the proposed method.

Study of the geomagnetic field's regional gradients in Chinese continent using three-dimensional surface Spline model

We combined domestic ground-based and satellite magnetic measurements to create a regional three-dimensional surface Spline(3DSS)gradient model of the main geomagnetic field over the Chinese continent.To improve the precision of the model,we considered the data gap between the ground and satellite data.We compared and analyzed the results of the Taylor polynomial,surface Spline,and CHAOS-6(the CHAMP,?rsted and SAC-C model of Earth’s magnetic field)gradient models.Results showed that the gradients in the south-north and east-west directions of the four models were consistent.The 3DSS model was able to express not only gradients at different altitudes,but also average gradients inside the research area.The two Spline models were able to capture more information on gradient anomalies than were the fitted models.Strong local anomalies were observed in northern Xinjiang,Beijing,and the junction area between Jiangsu and Zhejiang,and the total intensity F decreased whereas the altitude increased.The gradient decreased by 21.69%in the south-north direction and increased by 11.78%in the east-west direction.In addition,the altitude gradient turned from negative to positive while the altitude increased.The Spline model and the two fitted models differed mainly in the field sources they expressed and the modeling theory.

Can preoperative planning using IRIS™three-dimensional anatomical virtual models predict operative findings during robot-assisted partial nephrectomy?

Objective To evaluate the predictive validity of IRIS™(Intuitive Surgical®,Sunnyvale,CA,USA)as a planning tool for robot-assisted partial nephrectomy(RAPN)by assessing the degree of overlap with intraoperative execution.Methods Thirty-one patients scheduled for RAPN by four experienced urologists were enrolled in a prospective study.Prior to surgery,urologists reviewed the IRIS™three-dimensional model on an iphone Operating System(iOS)app and completed a questionnaire outlining their surgical plan including surgical approach,and ischemia technique as well as confidence in executing this plan.Postoperatively,questionnaires assessing the procedural approach,clinical utility,efficiency,and effectiveness of IRIS™were completed.The degree of overlap between the preoperative and intraoperative questionnaires and between the planned approach and actual execution of the procedure was analyzed.Questionnaires were answered on a 5-point Likert scale and scores of 4 or greater were considered positive.Results Mean age was 65.1 years with a mean tumor size of 27.7 mm(interquartile range 17.5-44.0 mm).Hilar tumors consisted of 32.3%;48.4%of patients had R.E.N.A.L.nephrometry scores of 7-9.On preoperative questionnaires,the surgeons reported that in 67.7%cases they were confident that they can perform the procedure successfully,and on intraoperative questionnaires,the surgeons reported that in 96.8%cases IRIS™helped achieve good spatial sensation of the anatomy.There was a high degree of overlap between preoperative and intraoperative questionnaires for the surgical approach,interpreting anatomical details and clinical utility.When comparing plans for selective or off-clamp,the preoperative plan was executed in 90.0%of cases intraoperatively.Conclusion A high degree of overlap between the preoperative surgical approach and intraoperative RAPN execution was found using IRIS™.This is the first study to evaluate the predictive accuracy of IRIS™during RAPN by comparing preoperative plan and intraoperative execution.

Three-dimensional numerical modeling of gravity anomalies based on Poisson equation in spacewavenumber mixed domain

In gravity-anomaly-based prospecting, the computational and memory requirements for practical numerical modeling are potentially enormous. Achieving an efficient and precise inversion for gravity anomaly imaging over large-scale and complex terrain requires additional methods. To this end, we have proposed a new topography-capable By performing a two-dimensional Fourier transform in the horizontal directions, threedimensional partial differential equations in the spatial domain were transformed into a group of independent, one-dimensional differential equations engaged with different wave numbers. These independent differential equations are highly parallel across different wave numbers. differential equations with different wave numbers, and the efficiency of solving fixedbandwidth linear equations was further improved by a chasing method. In a synthetic test, a prism model was used to verify the accuracy and reliability of the proposed algorithm by comparing the numerical solution with the analytical solution. We studied the computational precision and efficiency with and without topography using different Fourier transform methods. The results showed that the Guass-FFT method has higher numerical precision, while the standard FFT method is superior, in terms of computation time, for inversion and quantitative interpretation under complicated terrain.

Three-dimensional cell culture systems as an in vitro platform for cancer and stem cell modeling

Three-dimensional(3D)culture systems are becoming increasingly popular due to their ability to mimic tissue-like structures more effectively than the monolayer cultures.In cancer and stem cell research,the natural cell characteristics and architectures are closely mimicked by the 3D cell models.Thus,the 3D cell cultures are promising and suitable systems for various proposes,ranging from disease modeling to drug target identification as well as potential therapeutic substances that may transform our lives.This review provides a comprehensive compendium of recent advancements in culturing cells,in particular cancer and stem cells,using 3D culture techniques.The major approaches highlighted here include cell spheroids,hydrogel embedding,bioreactors,scaffolds,and bioprinting.In addition,the progress of employing 3D cell culture systems as a platform for cancer and stem cell research was addressed,and the prominent studies of 3D cell culture systems were discussed.

Three-dimensional land FD-CSEM forward modeling using edge finite-element method

A modeling tool for simulating three-dimensional land frequency-domain controlled-source electromagnetic surveys,based on a finite-element discretization of the Helmholtz equation for the electric fields,has been developed.The main difference between our modeling method and those previous works is edge finite-element approach applied to solving the three-dimensional land frequency-domain electromagnetic responses generated by horizontal electric dipole source.Firstly,the edge finite-element equation is formulated through the Galerkin method based on Helmholtz equation of the electric fields.Secondly,in order to check the validity of the modeling code,the numerical results are compared with the analytical solutions for a homogeneous half-space model.Finally,other three models are simulated with three-dimensional electromagnetic responses.The results indicate that the method can be applied for solving three-dimensional electromagnetic responses.The algorithm has been demonstrated,which can be effective to modeling the complex geo-electrical structures.This efficient algorithm will help to study the distribution laws of3-D land frequency-domain controlled-source electromagnetic responses and to setup basis for research of three-dimensional inversion.

Microstructure modeling and virtual test of asphalt mixture based on three-dimensional discrete element method

The objective of this work is to model the microstructure of asphalt mixture and build virtual test for asphalt mixture by using Particle Flow Code in three dimensions(PFC^(3D))based on three-dimensional discrete element method.A randomly generating algorithm was proposed to capture the three-dimensional irregular shape of coarse aggregate.And then,modeling algorithm and method for graded aggregates were built.Based on the combination of modeling of coarse aggregates,asphalt mastic and air voids,three-dimensional virtual sample of asphalt mixture was modeled by using PFC^(3D).Virtual tests for penetration test of aggregate and uniaxial creep test of asphalt mixture were built and conducted by using PFC^(3D).By comparison of the testing results between virtual tests and actual laboratory tests,the validity of the microstructure modeling and virtual test built in this study was verified.Additionally,compared with laboratory test,the virtual test is easier to conduct and has less variability.It is proved that microstructure modeling and virtual test based on three-dimensional discrete element method is a promising way to conduct research of asphalt mixture.

Three-dimensional forward modeling for magnetotelluric sounding by finite element method

A finite element algorithm combined with divergence condition was presented for computing three-dimensional(3D) magnetotelluric forward modeling. The finite element equation of three-dimensional magnetotelluric forward modeling was derived from Maxwell's equations using general variation principle. The divergence condition was added forcedly to the electric field boundary value problem, which made the solution correct. The system of equation of the finite element algorithm was a large sparse, banded, symmetric, ill-conditioned, non-Hermitian complex matrix equation, which can be solved using the Bi-CGSTAB method. In order to prove correctness of the three-dimensional magnetotelluric forward algorithm, the computed results and analytic results of one-dimensional geo-electrical model were compared. In addition, the three-dimensional magnetotelluric forward algorithm is given a further evaluation by computing COMMEMI model. The forward modeling results show that the algorithm is very efficient, and it has a lot of advantages, such as the high precision, the canonical process of solving problem, meeting the internal boundary condition automatically and adapting to all kinds of distribution of multi-substances.

Three-dimensional fuzzy logic system for process modeling and control

The traditional fuzzy logic system （FLS） can only model and control the process in two-dimensional nature. Many of real-world systems are of multidimensional features, such as, thermal and fluid processes with spatiotemporal dynamics, biological systems, or decision-making processes that contain stochastic and imprecise uncertainties. These types of systems are difficult for the traditional FLS to model and control because they require a third dimension for spatial or probabilistic information. The type-2 fuzzy set provides the possibility to develop a three-dimensional fuzzy logic system for modeling and controlling these processes in three-dimensional nature.

Three-Dimensional Simulations of RESET Operation in Phase-Change Random Access Memory with Blade-Type Like Phase Change Layer by Finite Element Modeling

An optimized device structure for reducing the RESET current of phase-change random access memory （PCRAM） with blade-type like （BTL） phase change layer is proposed. The electrical thermal analysis of the BTL cell and the blade heater contactor structure by three-dimensional finite element modeling are compared with each other during RESET operation. The simulation results show that the programming region of the phase change layer in the BTL cell is much smaller, and thermal electrical distributions of the BTL cell are more concentrated on the TiN/GST interface. The results indicate that the BTL cell has the superiorities of increasing the heating efficiency, decreasing the power consumption and reducing the RESET current from 0.67mA to 0.32mA. Therefore, the BTL cell will be appropriate for high performance PCRAM device with lower power consumption and lower RESET current.

The Design of a Three-Dimensional Physical Modeling System for Real-Time Groundwater Flows

In the past decades,physical modeling has been widely used in hydrogeology for teaching,studying and exhibition purposes.Most of these models are used to illustrate hydrogeological profiles,but few can depict three-dimensional groundwater flows,making it impossible to validate groundwater flows simulated by numerical methods with physical modeling.

Research on the Three-dimensional Modeling and Optimization of a Virtual Tower Crane Based on 3DS Max, Solidworks and EON Professional

Three-dimensional modeling of virtual hoisting machinery is the critical works to structure the system of virtual construction, and the foundation to realize intelligent and interactive virtual hoisting. Aimed at enhancing the requests of image quality and stability of the virtual construction scene, taking a tower crane for example. We studied the technology of three-dimensional modeling and optimization of a virtual tower crane, and a method named two-stage model optimization was put forward. This depended on the modeling stage using Solidworks and 3DS Max and the performance optimization stage in EON. The practice of software development indicates that the proposed methods of three-dimensional modeling and optimization could satisfy the performance request of virtual construction system and be popularized to other virtual system.

In vitro three-dimensional cancer metastasis modeling:Past,present,and future

Metastasis is the leading cause of most cancer deaths, as opposed to dysregulated cell growth of the primary tumor. Molecular mechanisms of metastasis have been studied for decades and the findings have evolved our understanding of the progression of malignancy. However, most of the molecular mechanisms fail to address the causes of cancer and its evolutionary origin, demonstrating an inability to find a solution for complete cure of cancer. After being a neglected area of tumor biology for quite some time, recently several studies have focused on the impact of the tumor microenvironment on cancer growth. The importance of the tumor microenvironment is gradually gaining attention, particularly from the per- spective of biophysics. In vitro three-dimensional （3-D） metastatic models are an indispensable platform for investigating the tumor microenvironment, as they mimic the in vivo tumor tissue. In 3-D metastatic in vitro models, static factors such as the mechanical properties, biochemical factors, as well as dynamic factors such as cell-cell, cell-ECM interactions, and fluid shear stress can be studied quantitatively. With increasing focus on basic cancer research and drug development, the in vitro 3-D models offer unique advantages in fundamental and clinical biomedical studies.

Central nervous system tumors and three-dimensional cell biology: Current and future perspectives in modeling

Central nervous system(CNS)tumors are a variety of distinct neoplasms that present multiple challenges in terms of treatment and prognosis.Glioblastoma,the most common primary tumor in adults,is associated with poor survival and remains one of the least treatable neoplasms.These tumors are highly heterogenous and complex in their nature.Due to this complexity,traditional cell culturing techniques and methods do not provide an ideal recapitulating model for the study of these tumors’behavior in vivo.Two-dimensional models lack the spatial arrangement,the heterogeneity in cell types,and the microenvironment that play a large role in tumor cell behavior and response to treatment.Recently,scientists have turned towards three-dimensional culturing methods,namely spheroids and organoids,as they have been shown to recapitulate tumors in a more faithful manner to their in vivo counterparts.Moreover,tumor-on-a-chip systems have lately been employed in CNS tumor modeling and have shown great potential in both studying the pathophysiology and therapeutic testing.In this review,we will discuss the current available literature on in vitro threedimensional culturing models in CNS tumors,in addition to presenting their advantages and current limitations.We will also elaborate on the future implications of these models and their benefit in the clinical setting.

Three-Dimensional Pressure Modeling of South China Sea in High Temperature High Pressure Field

Yingqiong basin is a proven hydrocarbon-rich basin in South China Sea. There are a number of large exploration prospects in high temperature and over-pressured formations, especially in Yacheng Block of Qiongdongnan basin and Dongfang District of Yinggehai Basin. Owing to good exploration situation, we have already achieved proven geological reserves over 1000 × 108 m3. In recent years, a few drilled HPHT wells have confirmed that pressure predicted by conventional method was wildly inaccurate. From the view of regional stress, the accuracy of the pressure prediction will be substantially improved. Accurate pressure prediction and three-dimensional pressure modeling which are based on three-dimensional lithology modeling are the cornerstone to achieve exploration breakthrough. In this paper, the use of the triple constraint trend lithology model broke through the traditional method of seismic lithology prediction only by means of impedance threshold value. Compared with actual data and prediction, it confirms that three-dimensional pressure modeling method is reasonable and effective, and has a wide prospect of application.