The issues of how to quickly collect seawater samples and of how to make sure that those samples truly reflect the in-situ information on gas composition and concentration have therefore become a hot but difficult top...The issues of how to quickly collect seawater samples and of how to make sure that those samples truly reflect the in-situ information on gas composition and concentration have therefore become a hot but difficult topic in the field of ocean technology.Most conventional seawater samplers only focus on collecting seawater itself,but take little consideration on gas preservation.A set of new oceanographic tools are presented for ocean resource exploration such as hydrothermal sulfide and gas hydrate,and for investigations on the processes and mechanisms of marine physical,chemical and biological evolutions.A gas-tight deep-sea water sampling system（GTWSS） is designed for the collection of deep-sea geochemical samples.This set of tools mainly consists of a conductivity temperature depth profiler（CTD）,release devices and gas-tight deep-sea water samplers（GTWS）.The GTWS is able to hold the gases in deep-sea water samples tightly,providing in-situ information on gas contents in the seawater samples and can be deployed on a routine wire-deployed CTD sampler for multi-layer discrete sampling of gas-tight seawater.Sea trials are performed successfully in 2008 and 2009,on a research vessel named HaiYang Si Hao in South China Sea,with the deepest trial depth 3 930 m.GTWSS is capable of quickly sampling 12 discrete gas-tight seawater samples（8.3 L per sample） during its single deployment.The head space method is employed to separate the gases from the seawater samples immediately after recovery of the seawater samples on the vessel.Field geochemical analysis is carried out by gaseous hydrocarbon sensors and an infrared gas analyzer.Results show that the concentrations of CH4 and CO2 in the seawater sampled by GTWSS are higher than those sampled by general non-gas-tight water samplers,thus confirming the gas tightness of GTWSS.Seawater samples can be collected quickly by using GTWSS,and GTWSS can keep the samples＇ integrity quite well.展开更多
Aiming at the requirement of the full-ocean-depth(operating water depth 11000 m)manned submersible to carry out the gas-tight sampling operation of the abyss seabed sediment,a kind of full-ocean-depth carrier submersi...Aiming at the requirement of the full-ocean-depth(operating water depth 11000 m)manned submersible to carry out the gas-tight sampling operation of the abyss seabed sediment,a kind of full-ocean-depth carrier submersible mechanical hand-held,full-ocean-depth gas-tight sediment sampler(GTSS)with the function of pressure-retaining and coring is designed.Firstly,the volume change model of pressure compensator is established,and it is pointed out that the volume of pressure compensator is about 16.14%equal to the volume of gas-tight sediment sampler(GTSS).Secondly,the pressure compensator is analyzed and calculated,and the relationship between the precharge pressure of the pressure compensator,the nominal volume of the pressure compensator and the pressure holding effect of the gas-tight sediment sampler(GTSS)is studied.The results show that with the increase of gas precharge pressure in the pressure compensator,the final pressure of the sampler also increases.Under the same precharge pressure condition,the larger the nominal volume of the pressure compensator,the greater the final pressure of the sampler.Finally,the air tightness test method is designed by using the developed gas tightness sampler of the full-ocean-depth product,and the change of the final pressure in the gas tight sampler under different precharge pressure is observed.The test results are consistent with the simulation results,indicating the correctness of the pressure compensation system(PCS)model.展开更多
This paper describes the design, construction and preliminary test results for a gas-tight serial sampler intended to be deployed at seafloor for long-term operation to take time-series fluid samples from deep-sea env...This paper describes the design, construction and preliminary test results for a gas-tight serial sampler intended to be deployed at seafloor for long-term operation to take time-series fluid samples from deep-sea environments such as cold seeps, water column and hydrothermal vents. The serial sampler is a modular system that is based on independent and identical sampling modules, which are designed to collect six 160 ml gas-tight fluid samples maintained at high pressure to a depth of 4000 meters. With two working modes, the sampler can be deployed either with seafloor cabled observatory for remote control or as a stand-alone device for autonomous operation. A prototype of the instrument has been constructed and tested on the MARS cabled observatory for two months. The laboratory and field tests proved the success of the design and construction of the serial sampler, and indicated the potential for future ocean sciences.展开更多
风蚀监测可反映风沙运动规律,集沙仪是风蚀监测的必备仪器,而以往研究大多在沙漠或沙地开展,较少涉及黑土农田。本研究基于BSNE(Big Spring Number Eight)集沙仪,将采沙盒底部阻风结构中的18目孔径筛网改进为逆向百叶窗式的阻风挡板,采...风蚀监测可反映风沙运动规律,集沙仪是风蚀监测的必备仪器,而以往研究大多在沙漠或沙地开展,较少涉及黑土农田。本研究基于BSNE(Big Spring Number Eight)集沙仪,将采沙盒底部阻风结构中的18目孔径筛网改进为逆向百叶窗式的阻风挡板,采用模拟试验对比分析BSNE集沙仪和改进后rBSNE(Revised Spring Number Eight)集沙仪的保沙和集沙能力,并将其应用于野外黑土农田风蚀监测。研究结果表明:与BSNE集沙仪相比,rBSNE集沙仪降低了小粒级颗粒的损失率,当风速低于20 m·s^(-1)时,对<0.25 mm粒径的土壤颗粒的损失率显著降低74.3%~87.1%;当风速达到20 m·s^(-1)时,对所有试验粒径的土壤颗粒的损失率显著降低46.9%~74.3%。与BSNE集沙仪相比,rBSNE集沙仪有助于对小粒级颗粒的收集,当风速低于20 m·s^(-1)时,对粒径为<0.5 mm的集沙量显著增加了9.0%~44.0%;当风速达到20 m·s^(-1)时,对所有试验粒径的集沙量均显著增加7.3%~34.4%。野外监测结果显示风蚀输沙量随高度增加呈指数函数递减趋势,说明rBSNE集沙仪可应用于黑土农田风蚀的监测。展开更多
Background Many phenotypes in animal breeding are derived from incomplete measures,especially if they are challenging or expensive to measure precisely.Examples include time-dependent traits such as reproductive statu...Background Many phenotypes in animal breeding are derived from incomplete measures,especially if they are challenging or expensive to measure precisely.Examples include time-dependent traits such as reproductive status,or lifespan.Incomplete measures for these traits result in phenotypes that are subject to left-,interval-and rightcensoring,where phenotypes are only known to fall below an upper bound,between a lower and upper bound,or above a lower bound respectively.Here we compare three methods for deriving phenotypes from incomplete data using age at first elevation(>1 ng/mL)in blood plasma progesterone(AGEP4),which generally coincides with onset of puberty,as an example trait.Methods We produced AGEP4 phenotypes from three blood samples collected at about 30-day intervals from approximately 5,000 Holstein–Friesian or Holstein–Friesian×Jersey cross-bred dairy heifers managed in 54 seasonal-calving,pasture-based herds in New Zealand.We used these actual data to simulate 7 different visit scenarios,increasing the extent of censoring by disregarding data from one or two of the three visits.Three methods for deriving phenotypes from these data were explored:1)ordinal categorical variables which were analysed using categorical threshold analysis;2)continuous variables,with a penalty of 31 d assigned to right-censored phenotypes;and 3)continuous variables,sampled from within a lower and upper bound using a data augmentation approach.Results Credibility intervals for heritability estimations overlapped across all methods and visit scenarios,but estimated heritabilities tended to be higher when left censoring was reduced.For sires with at least 5 daughters,the correlations between estimated breeding values(EBVs)from our three-visit scenario and each reduced data scenario varied by method,ranging from 0.65 to 0.95.The estimated breed effects also varied by method,but breed differences were smaller as phenotype censoring increased.Conclusion Our results indicate that using some methods,phenotypes derived from one observation per offspring for a time-dependent trait such as AGEP4 may provide comparable sire rankings to three observations per offspring.This has implications for the design of large-scale phenotyping initiatives where animal breeders aim to estimate variance parameters and estimated breeding values(EBVs)for phenotypes that are challenging to measure or prohibitively expensive.展开更多
基金supported by National Hi-tech Research and Development Program of China(863 Program,Grant Nos. 2006AA09A204-1,2006AA09Z222-1,2009AA09A20401-1)
文摘The issues of how to quickly collect seawater samples and of how to make sure that those samples truly reflect the in-situ information on gas composition and concentration have therefore become a hot but difficult topic in the field of ocean technology.Most conventional seawater samplers only focus on collecting seawater itself,but take little consideration on gas preservation.A set of new oceanographic tools are presented for ocean resource exploration such as hydrothermal sulfide and gas hydrate,and for investigations on the processes and mechanisms of marine physical,chemical and biological evolutions.A gas-tight deep-sea water sampling system（GTWSS） is designed for the collection of deep-sea geochemical samples.This set of tools mainly consists of a conductivity temperature depth profiler（CTD）,release devices and gas-tight deep-sea water samplers（GTWS）.The GTWS is able to hold the gases in deep-sea water samples tightly,providing in-situ information on gas contents in the seawater samples and can be deployed on a routine wire-deployed CTD sampler for multi-layer discrete sampling of gas-tight seawater.Sea trials are performed successfully in 2008 and 2009,on a research vessel named HaiYang Si Hao in South China Sea,with the deepest trial depth 3 930 m.GTWSS is capable of quickly sampling 12 discrete gas-tight seawater samples（8.3 L per sample） during its single deployment.The head space method is employed to separate the gases from the seawater samples immediately after recovery of the seawater samples on the vessel.Field geochemical analysis is carried out by gaseous hydrocarbon sensors and an infrared gas analyzer.Results show that the concentrations of CH4 and CO2 in the seawater sampled by GTWSS are higher than those sampled by general non-gas-tight water samplers,thus confirming the gas tightness of GTWSS.Seawater samples can be collected quickly by using GTWSS,and GTWSS can keep the samples＇ integrity quite well.
基金Supported by National Key Research and Development Program of China(Grant No.2016YFC0300502)National Natural Science Foundation of China(Grant No.517779092)+1 种基金Special Project for the Construction of Innovative Provinces in Hunan(Grant No.2019GK1012)Postgraduate Scientific Research Innovation Project of Hunan Province(Grant No.CX20210985).
文摘Aiming at the requirement of the full-ocean-depth(operating water depth 11000 m)manned submersible to carry out the gas-tight sampling operation of the abyss seabed sediment,a kind of full-ocean-depth carrier submersible mechanical hand-held,full-ocean-depth gas-tight sediment sampler(GTSS)with the function of pressure-retaining and coring is designed.Firstly,the volume change model of pressure compensator is established,and it is pointed out that the volume of pressure compensator is about 16.14%equal to the volume of gas-tight sediment sampler(GTSS).Secondly,the pressure compensator is analyzed and calculated,and the relationship between the precharge pressure of the pressure compensator,the nominal volume of the pressure compensator and the pressure holding effect of the gas-tight sediment sampler(GTSS)is studied.The results show that with the increase of gas precharge pressure in the pressure compensator,the final pressure of the sampler also increases.Under the same precharge pressure condition,the larger the nominal volume of the pressure compensator,the greater the final pressure of the sampler.Finally,the air tightness test method is designed by using the developed gas tightness sampler of the full-ocean-depth product,and the change of the final pressure in the gas tight sampler under different precharge pressure is observed.The test results are consistent with the simulation results,indicating the correctness of the pressure compensation system(PCS)model.
基金financially supported by the National High Technology Research and Development Program of China(863 Program,Grant No.2010AA09Z202)the National Natural Science Foundation of China(Grant No.41106081)+2 种基金the Science Fund for Creative Research Groups of the National Natural Science Foundation of China(Grant No.51221004)the United States National Science Foundation(Grant No.0927615)the Knowledge Innovation Project from Sanya Institute of Deep-sea Science and Engineering(Grant No.SIDSSE-201204)
文摘This paper describes the design, construction and preliminary test results for a gas-tight serial sampler intended to be deployed at seafloor for long-term operation to take time-series fluid samples from deep-sea environments such as cold seeps, water column and hydrothermal vents. The serial sampler is a modular system that is based on independent and identical sampling modules, which are designed to collect six 160 ml gas-tight fluid samples maintained at high pressure to a depth of 4000 meters. With two working modes, the sampler can be deployed either with seafloor cabled observatory for remote control or as a stand-alone device for autonomous operation. A prototype of the instrument has been constructed and tested on the MARS cabled observatory for two months. The laboratory and field tests proved the success of the design and construction of the serial sampler, and indicated the potential for future ocean sciences.
基金Project supported by the National Natural Science Foundation of China （No. 41106081）, and the Science Fund for Creative Research Groups of the National Natural Science Foundation of China （No. 51221004）
文摘风蚀监测可反映风沙运动规律,集沙仪是风蚀监测的必备仪器,而以往研究大多在沙漠或沙地开展,较少涉及黑土农田。本研究基于BSNE(Big Spring Number Eight)集沙仪,将采沙盒底部阻风结构中的18目孔径筛网改进为逆向百叶窗式的阻风挡板,采用模拟试验对比分析BSNE集沙仪和改进后rBSNE(Revised Spring Number Eight)集沙仪的保沙和集沙能力,并将其应用于野外黑土农田风蚀监测。研究结果表明:与BSNE集沙仪相比,rBSNE集沙仪降低了小粒级颗粒的损失率,当风速低于20 m·s^(-1)时,对<0.25 mm粒径的土壤颗粒的损失率显著降低74.3%~87.1%;当风速达到20 m·s^(-1)时,对所有试验粒径的土壤颗粒的损失率显著降低46.9%~74.3%。与BSNE集沙仪相比,rBSNE集沙仪有助于对小粒级颗粒的收集,当风速低于20 m·s^(-1)时,对粒径为<0.5 mm的集沙量显著增加了9.0%~44.0%;当风速达到20 m·s^(-1)时,对所有试验粒径的集沙量均显著增加7.3%~34.4%。野外监测结果显示风蚀输沙量随高度增加呈指数函数递减趋势,说明rBSNE集沙仪可应用于黑土农田风蚀的监测。
基金funded by New Zealand dairy farmers through Dairy NZ Inc. and by the New Zealand Ministry of Business,Innovation and Employment (DRCX1302)support was kindly received from LIC and CRV
文摘Background Many phenotypes in animal breeding are derived from incomplete measures,especially if they are challenging or expensive to measure precisely.Examples include time-dependent traits such as reproductive status,or lifespan.Incomplete measures for these traits result in phenotypes that are subject to left-,interval-and rightcensoring,where phenotypes are only known to fall below an upper bound,between a lower and upper bound,or above a lower bound respectively.Here we compare three methods for deriving phenotypes from incomplete data using age at first elevation(>1 ng/mL)in blood plasma progesterone(AGEP4),which generally coincides with onset of puberty,as an example trait.Methods We produced AGEP4 phenotypes from three blood samples collected at about 30-day intervals from approximately 5,000 Holstein–Friesian or Holstein–Friesian×Jersey cross-bred dairy heifers managed in 54 seasonal-calving,pasture-based herds in New Zealand.We used these actual data to simulate 7 different visit scenarios,increasing the extent of censoring by disregarding data from one or two of the three visits.Three methods for deriving phenotypes from these data were explored:1)ordinal categorical variables which were analysed using categorical threshold analysis;2)continuous variables,with a penalty of 31 d assigned to right-censored phenotypes;and 3)continuous variables,sampled from within a lower and upper bound using a data augmentation approach.Results Credibility intervals for heritability estimations overlapped across all methods and visit scenarios,but estimated heritabilities tended to be higher when left censoring was reduced.For sires with at least 5 daughters,the correlations between estimated breeding values(EBVs)from our three-visit scenario and each reduced data scenario varied by method,ranging from 0.65 to 0.95.The estimated breed effects also varied by method,but breed differences were smaller as phenotype censoring increased.Conclusion Our results indicate that using some methods,phenotypes derived from one observation per offspring for a time-dependent trait such as AGEP4 may provide comparable sire rankings to three observations per offspring.This has implications for the design of large-scale phenotyping initiatives where animal breeders aim to estimate variance parameters and estimated breeding values(EBVs)for phenotypes that are challenging to measure or prohibitively expensive.
