胃内菌群与胃癌(gastric cancer,GC)的关系是近年来的研究热点之一.研究发现胃内菌群通过多种途径参与GC发生,且在肿瘤发展过程中出现特征性改变.幽门螺杆菌被认为是Ⅰ类致癌原,在GC发展中发挥重要作用.近年来,越来越多研究发现非幽门...胃内菌群与胃癌(gastric cancer,GC)的关系是近年来的研究热点之一.研究发现胃内菌群通过多种途径参与GC发生,且在肿瘤发展过程中出现特征性改变.幽门螺杆菌被认为是Ⅰ类致癌原,在GC发展中发挥重要作用.近年来,越来越多研究发现非幽门螺杆菌同样参与GC发展.本文通过检索知网、PubMed、Web of Science,围绕胃内微生态与GC的相互关系、作用机制及临床价值展开综述,以期为GC的临床诊疗提供新思路.展开更多
BACKGROUND Helicobacter pylori(H.pylori)is a spiral-shaped bacterium responsible for the development of chronic gastritis,gastric ulcer,gastric cancer(GC),and MALTlymphoma of the stomach.H.pylori can be present in the...BACKGROUND Helicobacter pylori(H.pylori)is a spiral-shaped bacterium responsible for the development of chronic gastritis,gastric ulcer,gastric cancer(GC),and MALTlymphoma of the stomach.H.pylori can be present in the gastric mucosa(GM)in both spiral and coccoid forms.However,it is not known whether the severity of GM contamination by various vegetative forms of H.pylori is associated with clinical and morphological characteristics and long-term results of GC treatment.AIM To establish the features of H.pylori infection in patients with GC and their correlations with clinical and morphological characteristics of diseases and long-term results of treatment.METHODS Of 109 patients with GC were included in a prospective cohort study.H.pylori in the GM and tumor was determined by rapid urease test and by immunohistochemically using the antibody to H.pylori.The results obtained were compared with the clinical and morphological characteristics and prognosis of GC.Statistical analysis was performed using the Statistica 10.0 software.RESULTS H.pylori was detected in the adjacent to the tumor GM in 84.5%of cases,of which a high degree of contamination was noted in 50.4%of the samples.Coccoid forms of H.pylori were detected in 93.4%of infected patients,and only coccoid-in 68.9%.It was found that a high degree of GM contamination by the coccoid forms of H.pylori was observed significantly more often in diffuse type of GC(P=0.024),in poorly differentiated GC(P=0.011),in stage T3-4(P=0.04)and in N1(P=0.011).In cases of moderate and marked concentrations of H.pylori in GM,a decrease in 10-year relapse free and overall survival from 55.6%to 26.3%was observed(P=0.02 and P=0.07,respectively).The relationship between the severity of the GM contamination by the spiral-shaped forms of H.pylori and the clinical and morphological characteristics and prognosis of GC was not revealed.CONCLUSION The data obtained indicates that H.pylori may be associated not only with induction but also with the progression of GC.展开更多
主要研究了盐诱导大豆分离蛋白(soy protein isolate,SPI)形成的冷凝胶对乳酸菌在胃酸pH下的保护作用。用CaCl2作为凝胶剂诱导大豆分离蛋白成胶,以30 min时乳酸菌在胃酸pH下的存活率作为评价指标,来评价冷凝胶对细菌的抗酸保护作用。变...主要研究了盐诱导大豆分离蛋白(soy protein isolate,SPI)形成的冷凝胶对乳酸菌在胃酸pH下的保护作用。用CaCl2作为凝胶剂诱导大豆分离蛋白成胶,以30 min时乳酸菌在胃酸pH下的存活率作为评价指标,来评价冷凝胶对细菌的抗酸保护作用。变性温度为85℃、SPI浓度为9%以及CaCl2浓度为20 mmol/L时所形成的冷凝胶,在pH1.2的胃酸环境下对乳酸菌的保护性最好,活菌落的数量级由初始的107变为102,下降了5个数量级。说明大豆分离蛋白冷凝胶对乳酸菌有良好的保护性,并为益生菌在食品中的开发和应用提供了非常好的前景。展开更多
文摘胃内菌群与胃癌(gastric cancer,GC)的关系是近年来的研究热点之一.研究发现胃内菌群通过多种途径参与GC发生,且在肿瘤发展过程中出现特征性改变.幽门螺杆菌被认为是Ⅰ类致癌原,在GC发展中发挥重要作用.近年来,越来越多研究发现非幽门螺杆菌同样参与GC发展.本文通过检索知网、PubMed、Web of Science,围绕胃内微生态与GC的相互关系、作用机制及临床价值展开综述,以期为GC的临床诊疗提供新思路.
文摘BACKGROUND Helicobacter pylori(H.pylori)is a spiral-shaped bacterium responsible for the development of chronic gastritis,gastric ulcer,gastric cancer(GC),and MALTlymphoma of the stomach.H.pylori can be present in the gastric mucosa(GM)in both spiral and coccoid forms.However,it is not known whether the severity of GM contamination by various vegetative forms of H.pylori is associated with clinical and morphological characteristics and long-term results of GC treatment.AIM To establish the features of H.pylori infection in patients with GC and their correlations with clinical and morphological characteristics of diseases and long-term results of treatment.METHODS Of 109 patients with GC were included in a prospective cohort study.H.pylori in the GM and tumor was determined by rapid urease test and by immunohistochemically using the antibody to H.pylori.The results obtained were compared with the clinical and morphological characteristics and prognosis of GC.Statistical analysis was performed using the Statistica 10.0 software.RESULTS H.pylori was detected in the adjacent to the tumor GM in 84.5%of cases,of which a high degree of contamination was noted in 50.4%of the samples.Coccoid forms of H.pylori were detected in 93.4%of infected patients,and only coccoid-in 68.9%.It was found that a high degree of GM contamination by the coccoid forms of H.pylori was observed significantly more often in diffuse type of GC(P=0.024),in poorly differentiated GC(P=0.011),in stage T3-4(P=0.04)and in N1(P=0.011).In cases of moderate and marked concentrations of H.pylori in GM,a decrease in 10-year relapse free and overall survival from 55.6%to 26.3%was observed(P=0.02 and P=0.07,respectively).The relationship between the severity of the GM contamination by the spiral-shaped forms of H.pylori and the clinical and morphological characteristics and prognosis of GC was not revealed.CONCLUSION The data obtained indicates that H.pylori may be associated not only with induction but also with the progression of GC.
文摘主要研究了盐诱导大豆分离蛋白(soy protein isolate,SPI)形成的冷凝胶对乳酸菌在胃酸pH下的保护作用。用CaCl2作为凝胶剂诱导大豆分离蛋白成胶,以30 min时乳酸菌在胃酸pH下的存活率作为评价指标,来评价冷凝胶对细菌的抗酸保护作用。变性温度为85℃、SPI浓度为9%以及CaCl2浓度为20 mmol/L时所形成的冷凝胶,在pH1.2的胃酸环境下对乳酸菌的保护性最好,活菌落的数量级由初始的107变为102,下降了5个数量级。说明大豆分离蛋白冷凝胶对乳酸菌有良好的保护性,并为益生菌在食品中的开发和应用提供了非常好的前景。