In this study, an Alzheimer＇s disease model was established in rats through stereotactic injection of condensed amyloid beta 1-40 into the bilateral hippocampus, and the changes of gene expression profile in the hipp...In this study, an Alzheimer＇s disease model was established in rats through stereotactic injection of condensed amyloid beta 1-40 into the bilateral hippocampus, and the changes of gene expression profile in the hippocampus of rat models and sham-operated rats were compared by genome expression profiling analysis. Results showed that the expression of 50 genes was significantly up-regulated （fold change 〉 2）, while 21 genes were significantly down-regulated in the hippocampus of Alzheimer＇s disease model rats （fold change 〈 0.5） compared with the sham-operation group. The differentially expressed genes are involved in many functions, such as brain nerve system development, neuronal differentiation and functional regulation, cellular growth, differentiation and apoptosis, synaptogenesis and plasticity, inflammatory and immune responses, ion channels/transporters, signal transduction, cell material/energy metabolism. Our findings indicate that several genes were abnormally expressed in the metabolic and signal transduction pathways in the hippocampus of amyloid beta 1 40-induced rat model of Alzheimer＇s disease, thereby affecting the hippocampal and brain functions.展开更多
基因测序仪在读取基因序列之前需要将镜头与基因芯片精准对齐,提出了一种能够精确地计算视场(Field of View,FOV)与理想位置偏差的算法.预先在基因芯片上的特定位置设置标记,通过拍摄的图像分析视场与基因芯片的位置误差:首先,提取图像...基因测序仪在读取基因序列之前需要将镜头与基因芯片精准对齐,提出了一种能够精确地计算视场(Field of View,FOV)与理想位置偏差的算法.预先在基因芯片上的特定位置设置标记,通过拍摄的图像分析视场与基因芯片的位置误差:首先,提取图像灰度特征捕捉标记位置以初步对齐视场中心位置;其次,捕捉标记上的多个关键点的坐标;最后,对关键点的坐标映射关系进行拟合,即可计算出精确的坐标和角度偏差.实践和实验分析表明,使用设计的图像配准算法能够实现对视场与基因芯片间位置偏差计算的高精度估计.展开更多
基金sponsored by the National Natural Science Foundation of China,No. 30973779
文摘In this study, an Alzheimer＇s disease model was established in rats through stereotactic injection of condensed amyloid beta 1-40 into the bilateral hippocampus, and the changes of gene expression profile in the hippocampus of rat models and sham-operated rats were compared by genome expression profiling analysis. Results showed that the expression of 50 genes was significantly up-regulated （fold change 〉 2）, while 21 genes were significantly down-regulated in the hippocampus of Alzheimer＇s disease model rats （fold change 〈 0.5） compared with the sham-operation group. The differentially expressed genes are involved in many functions, such as brain nerve system development, neuronal differentiation and functional regulation, cellular growth, differentiation and apoptosis, synaptogenesis and plasticity, inflammatory and immune responses, ion channels/transporters, signal transduction, cell material/energy metabolism. Our findings indicate that several genes were abnormally expressed in the metabolic and signal transduction pathways in the hippocampus of amyloid beta 1 40-induced rat model of Alzheimer＇s disease, thereby affecting the hippocampal and brain functions.
文摘基因测序仪在读取基因序列之前需要将镜头与基因芯片精准对齐,提出了一种能够精确地计算视场(Field of View,FOV)与理想位置偏差的算法.预先在基因芯片上的特定位置设置标记,通过拍摄的图像分析视场与基因芯片的位置误差:首先,提取图像灰度特征捕捉标记位置以初步对齐视场中心位置;其次,捕捉标记上的多个关键点的坐标;最后,对关键点的坐标映射关系进行拟合,即可计算出精确的坐标和角度偏差.实践和实验分析表明,使用设计的图像配准算法能够实现对视场与基因芯片间位置偏差计算的高精度估计.
