Isolation and cloning of the gene encoding high affinity phosphate transporter in rice

High affinity phosphate transporterplays an important role in plantadapting to low phosphorus. Isolationof genes coding this kind of proteinhas attracted worldwide scholars toaccomplish. We aimed to isolate thegene and transfer it to target plants

Isolation and Characterization of the Cytoplasmic Male Sterility Associated Gene of Cotton(Gossypium harknessii)

Cytoplasmic male sterility(CMS) is a maternally inherited trait that results in the failure to produce functional pollen.It was identified in many plants,and it is widely used to exploit heterosis.

Characterization of Class 1 Integron Gene Cassettes among Clinical Bacteria Isolated from One Large Hospital in Northern China

The class 1 integron and complex gene cassettes among different species of clinical isolates in northern China were characterized in this study. 383 clinical isolates were obtained from northern China, and class 1 integrons containing gene cassettes widely distributed among gram negative clinical isolates was observed. We find that the class 1 integron showed positive correlation with multidrug resistance phenotype of gram negative bacteria. In addition, we find that isolates belonged to one species harbored different types of gene cassette arrays, while same types of gene cassette arrays were observed in different species of isolates. The diversity of gene cassette arrays among the isolates indicated the complexity of multidrug resistance in clinical isolates in northern China.

Intracellular Interactions in the Reproduction Control of Introgressed Strains Involving Species from Drosophila saltans Group(Sophophora Subgenus)with Emphasis to the Effect of Wolbachia Infection

Interspecific hybrids and constructed research models have provided infor-mation on intracellular interactions. We used two introgressed, chromosomally differentiated strains (H4 and H2) derived from F1 hybrids of crosses between D. prosaltans females and D. saltans males. In H4, the D. prosaltans chromosomes were eliminated. In H2, a mixture was maintained, preserving the entire genome of D. prosaltans (except the Y chromosome) and parts of the D. saltans genome. The IIR arm and a segment of chromosome III were eliminated. A third strain, used for comparison, was a normal D. prosaltans strain (P). This study aimed primarily to analyze the effect on the reproductive char-acteristics productivity (number of progeny) and sex-ratio caused by Wolbachia infection in interaction with different chromosome constitutions. For this, infected and uninfected flies were used in intrastrain cross combinations. Firstly, we analyzed the productivity of intracrosses of uninfected parents, in each strain, in order to detect the effects of intracellular interactions, in flies carrying different chromosome constitutions and sharing a Wolbach-ia-free, D. prosaltans cytoplasm. Data indicated that the chromosome parts that were eliminated, in H2, carry the isolating genes that impair productivity in hybrids of the two species, and suggested the occurrence of a nuclear/nuclear interaction. The analysis of Wolbachia-infected flies showed that the three strains presented different responses, depending on the chromosome constitution. As to productivity, the infection was harmful in P strain, in H2 behaved as mutualistic, and, in H4, produced the effect cytoplasmic incompatibility. As to sex-ratio, intracrosses showed significant differences in P and H4 strains. These results, associated with the cytological characteristics of the strains, pointed to the fundamental importance of host chromosome constitution to define the interactive process host/Wolbachia, and showed the flexibility of the endo-symbiont manifested in different forms of self-preservation.