AIM: To detect the expression of p33^ING1b protein and the change of p33^ING1b gene in pancreatic carcinoma and to evaluate the significance of p33^ING1b in pancreatic cell carcinogenesis.METHODS: Pathological specime...AIM: To detect the expression of p33^ING1b protein and the change of p33^ING1b gene in pancreatic carcinoma and to evaluate the significance of p33^ING1b in pancreatic cell carcinogenesis.METHODS: Pathological specimens from pancreatic carcinoma and matched non-tumor pancreatic tissues were examined for p33^ING1b expression and mutation by immunohistochemistry, polymerase chain reaction singlestrand conformation polymorphisms (PCR-SSCP) and loss of heterozygosity (LOH).RESULTS: The rate of p33^ING1b protein expression was 85% (34/40). A single germline missense mutation was detected in 1 of 40 tumors located at codon 215:TGC-TCC (Cys-Ser).i-ourteen (60.9%) of 23 tumor samples snowea LUH In all of the informative markers tested, but no mutation was detected in these tumors and only two of the informative tumors lacked expressions of p33^ING1b protein.CONCLUSION: Mutation and loss of expression are not the main reasons for the disfunction of p33^ING1b in pancreatic carcinoma, an abnormality at the level of chromosome and/or transcription may inhibit their normal functions,potentially contributing to pancreatic cell carcinogenesis.展开更多
目的:探讨生长抑制基因(inhibitor of growth,p33ING1)在结直肠癌中的表达意义,以及p33ING1与结直肠癌临床病理特征的关系及其可能的作用机制。方法:对60例结直肠癌组织及20例癌旁非肿瘤组织标本,应用免疫组化检测肿瘤组织和正常组织的p...目的:探讨生长抑制基因(inhibitor of growth,p33ING1)在结直肠癌中的表达意义,以及p33ING1与结直肠癌临床病理特征的关系及其可能的作用机制。方法:对60例结直肠癌组织及20例癌旁非肿瘤组织标本,应用免疫组化检测肿瘤组织和正常组织的p33ING1、p21WAF1的表达。结果:p33ING1在结直肠癌组织中的表达阳性率为43.3%,在正常组织中为90.0%,显著低于正常组织(P<0.005);p33ING1减低与结直肠癌的Dukes分期及淋巴结转移有一定关系(P<0.005和P<0.01),而与患者的性别、年龄、肿瘤分化程度、肿瘤部位及浸润深度无明显关系(P>0.05)。p33ING1与p21WAF1表达呈正相关关系(P<0.05)。结论:p33ING1在结直肠癌组织中低表达,对结直肠癌的发生、发展可能起着重要的作用;p33ING1、p21WAF1表达可作为判断结直肠癌恶性程度的重要指标之一。展开更多
基金Supported by the "258" Project Foundation of Changhai Hospital,Shanghai,China
文摘AIM: To detect the expression of p33^ING1b protein and the change of p33^ING1b gene in pancreatic carcinoma and to evaluate the significance of p33^ING1b in pancreatic cell carcinogenesis.METHODS: Pathological specimens from pancreatic carcinoma and matched non-tumor pancreatic tissues were examined for p33^ING1b expression and mutation by immunohistochemistry, polymerase chain reaction singlestrand conformation polymorphisms (PCR-SSCP) and loss of heterozygosity (LOH).RESULTS: The rate of p33^ING1b protein expression was 85% (34/40). A single germline missense mutation was detected in 1 of 40 tumors located at codon 215:TGC-TCC (Cys-Ser).i-ourteen (60.9%) of 23 tumor samples snowea LUH In all of the informative markers tested, but no mutation was detected in these tumors and only two of the informative tumors lacked expressions of p33^ING1b protein.CONCLUSION: Mutation and loss of expression are not the main reasons for the disfunction of p33^ING1b in pancreatic carcinoma, an abnormality at the level of chromosome and/or transcription may inhibit their normal functions,potentially contributing to pancreatic cell carcinogenesis.
文摘目的:探讨生长抑制基因(inhibitor of growth,p33ING1)在结直肠癌中的表达意义,以及p33ING1与结直肠癌临床病理特征的关系及其可能的作用机制。方法:对60例结直肠癌组织及20例癌旁非肿瘤组织标本,应用免疫组化检测肿瘤组织和正常组织的p33ING1、p21WAF1的表达。结果:p33ING1在结直肠癌组织中的表达阳性率为43.3%,在正常组织中为90.0%,显著低于正常组织(P<0.005);p33ING1减低与结直肠癌的Dukes分期及淋巴结转移有一定关系(P<0.005和P<0.01),而与患者的性别、年龄、肿瘤分化程度、肿瘤部位及浸润深度无明显关系(P>0.05)。p33ING1与p21WAF1表达呈正相关关系(P<0.05)。结论:p33ING1在结直肠癌组织中低表达,对结直肠癌的发生、发展可能起着重要的作用;p33ING1、p21WAF1表达可作为判断结直肠癌恶性程度的重要指标之一。
