Study on Genetic Toxicity of Solidago decurrens

[Objectives]This study was conducted to analyze the genetic toxicity of Solidago decurrens,with a view to scientifically and safely using S.decurrens.[Methods]Using commercially available S.decurrens as an experimental material,according to Vicia faba root tip micronucleus technique,V.faba root tips were treated with different doses of aqueous extract from S.decurrens for different times,and the mitotic index,frequency of micronucleus and chromosomal aberration of root tips in V.faba were detected,so as to reveal the allelopathic potential and the potential ecological risks of S.decurrens at the cellular level.[Results]The decrease in the mitotic index caused by the aqueous extract of S.decurrens was positively related to the concentration of the aqueous extract and the treatment time.The aqueous extract of S.decurrens increased the frequency of micronucleus in root tip cells of V.faba,and the increase was positively related to the concentration of the aqueous extract and the treatment time.The aqueous extract of S.decurrens caused abnormal phenomena such as chromosome bridges,chromosome fragmentation,and chromosome lag in V.faba root tip cells,and the effect of the aqueous extract on V.faba root tips had significant effects dependent on time and concentration.Above results indicate that the aqueous extract of S.decurrens inhibited and damaged the mitosis of V.faba root tip cells to some degrees,had certain genetic toxicity and had the ability to rapidly spread.[Conclusions]This study provides a theoretical basis for scientifically and rationally using S.decurrens and preventing the spread of S.decurrens.

Study on the Genetic and Physiological Toxicity of Wastewater from a Pharmaceutical Factory Using Root Tip Micronucleus Technology of Vicia faba

ObjectiveThe aim was to assess genetic and physiological toxicity of wastewater from a pharmaceutical factory using root tip micronucleus technology of Vicia faba. MethodThe pollution of wastewater from a pharmaceutical factory was detected by using root tip micronucleus technology of Vicia faba, and the genetic and physiological toxicity of the wastewater to Vicia faba was assessed. ResultNon-processed wastewater had an extremely high level of biological toxicity; the cells were unable to live with the wastewater at a high concentration; the cells were able to grow with the wastewater at a low concentration, though the micronucleus ratio was extremely high. The processed wastewater had no significant impact on cell growth, but the micronucleus ratio was extremely high, showing that the processed water also had a high pollution index. ConclusionThe research could provide scientific references for the national treatment of wastewater from a pharmaceutical factory.

Study on the Acute Toxicity and Genetics Toxicity of Bensulfuronk-methyl on Danio rerio

[Objective] The aim was to study the effect of bensulfuron-methyl herbicide on acute toxicity and genetics toxicity of Danio redo. [ Method] Median lethal concentration was calculated by acute toxicity test, and analyzing the herbicide whether existing in potential toxicity to aquatic organisms or not. Based on the study of acute toxicity, genetics toxicity was carried out, by calculating the micronucleus rate to judge bensulfuron-methyl herbicide whether existing in potential toxicity or not. [ Result ] The LD5o （24 h and 48 h） of bensulfuron-methyl herbicide are 0.698 ml/L and 0.637 ml/L respectively, the safe concentration was 0.159 ml/L. The results on the effects of micronucleus （MN） in erythrocytes of Danio redo induced by bensulfuron-methyl at different times and different concentrations showed that the MN rate of control group was 0.010 3%, the highest MN rate of experimental group reached to 0. 372%, it also indicated that bensulfuron-methyl herbicide had genetics toxicity to Danio redo. At the same detection time, there was dose-effect relationship of MN rate in erythrocytes between treatment and control groups with different concentrations. In the same treatment group, the MN rate in erythrocytes reached to peak value at 24 h, and decreased at 48 h and 72 h with the infection time was prolonged. [ Conclusion ] The study provides some basis for scientifically selecting and reasonably using herbicide.

Security evaluation of compounded microbial flocculant

A new kind of compounded microbial flocculant (CMBF) for water and wastewater treatment has been developed through biological technology. In order to discuss its biological security, four groups of experiments, rat acute toxicity test via mouth, salmonella assay in vitro, mouse micronucleus in vivo test and teratogenesis test were conducted to evaluate its general toxicity, genotoxicity and generative toxicity. The experimental results showed that this type of compounded microbial flocculant was a substantial non-toxic substance based on the fact that LD50 value was over 10 mg/kg. The results from salmonella in vivo test and mouse micronucleus in vivo test revealed that the compounded microbial flocculant is a genetically non-toxic substance. Furthermore, compounded microbial flocculant has little effect on the growth of all the rats, and any morphologic abnormal phenomenon hasn’t appeared.

Chemical identification and genotoxicity analysis of petrochemical industrial wastewater

The actual harmful effects of industrial waste- water can not be reflected by the conventional water quality index. Therefore, the change in dissolved organic matter and the genetic toxicity of petrochemical waste- water were observed in the current study by examining the wastewater treatment plant of a large petrochemical enterprise in Northwest China. Using XAD-8, MSC, and DA-7 resins, the wastewater was separated into six fractions, namely, hydrophobic acid （HOA）, hydrophobic neutral （HOB）, hydrophobic alkaline, hydrophilic acid, hydrophilic alkaline, and hydrophilic neutral. Umu-test was used to detect the genetic toxicity of the wastewater samples, and fluorescence spectra were also obtained to examine genetic toxic substances. The results show that wastewater treatment facilities can effectively reduce the concentration of organic matter in petrochemical waste- water （p 〈 0.05）. However, the mixing of aniline waste- water can increase the amount of organic carbon （p 〈 0.05） and can overload facilities. This finding shows that the mixed collection and joint treatment of different types of petrochemical wastewater can affect the water quality of the effluent. Particularly, hydrophobic substances can be difficult to remove and account for a relatively large proportion of the effluent. The mixture of aniline waste- water can increase the genetic toxicity of the effluent （p〈0.05）, and biologic treatment can not effectively decrease the toxicity. Most of the genetic toxicology may exist in the HOA and HOB fractions. Fluorescence spectroscopy also confirms this result, and tryptophan- like substances may play an important role in genetic toxicity.