AIMTo determine the association between chlamydial conjunctivitis and genital infection by Chlamydia trachomatis, Mycoplasma genitalium and Candida albicans, in addition to the possible relationship between cultured b...AIMTo determine the association between chlamydial conjunctivitis and genital infection by Chlamydia trachomatis, Mycoplasma genitalium and Candida albicans, in addition to the possible relationship between cultured bacterial pathogens and oculogenital chlamydial infection.METHODSThis study was performed on 100 (50 symptomatic and 50 asymptomatic) women attending the Gynecological and Obstetric outpatient clinic of Alzahra hospital, Alazhar University. Simultaneously a conjunctival swab was taken from these patients. Polymerase chain reaction (PCR) was done on DNA extracted from both vaginal and conjunctival swab samples. Culture for both vaginal and conjunctival swabs was also done.RESULTSCandida albicans was the predominant organism isolated by culture in 20% and 40% of conjunctival and vaginal swabs respectively. By the PCR method, ocular Chlamydia trachomatis was present in 60% of symptomatic women, while genital Chlamydia trachomatis infection was present in 30% of symptomatic women. The results of this method also indicated that 25/50 (50%) vaginal swabs were positive with PCR for Candida albicans versus 15/50 (30%) were PCR positive in conjunctival swab. Mycoplasma genitalium was present in only 10% of vaginal swabs. Concomitant oculogenital PCR positive results for Chlamydia trachomatis and Candida albicans were 30% and 28% respectively.CONCLUSIONOcular Chlamydia trachomatis was associated with genital Chlamydia trachomatis in a high percentage of women followed by Candida albicans. Cultured bacterial organisms do not play a role in enhancement of Chlamydia trachomatis infection.展开更多
Introduction: Recent studies suggest that infectious organisms may facilitate initiation and metastasis of many human cancers. One infectious organism of interest is Mycoplasma genitalium(Mg), a prevalent organism in ...Introduction: Recent studies suggest that infectious organisms may facilitate initiation and metastasis of many human cancers. One infectious organism of interest is Mycoplasma genitalium(Mg), a prevalent organism in humans known to cause sexually transmitted infection, as well as urethritis and prostatitis. Previous studies have demonstrated that benign, non-tumorigenic human prostate cells (BPH-1) chronically exposed to M. genitalium led to the malignant transformation of these cells as demonstrated in in vitro and in vivo models. Based on work from our laboratory, we felt this malignant transformation revolved around a specific M. genitalium’s ABC transporter (MG289) with homology to M. hyorhinis’ ABC transporter, p37. In this study, differences in M. genitalium’s ability to infect and induce a unique proteome conducive to tumoral growth were studied with engineered M. genitalium in which the p37 protein was silent. Materials and Methods: Wild-type M. genitalium (strain 431c, designated as M. genitalium WT) and MG289 deficient M. genitalium mutant (strain 260_3, designated as Mg260_3) were used for this study. We studied the infectivity potential between M. genitalium WT and Mg260_3 upon exposure to BPH-1 cells. Furthermore, we set out to identify a unique proteome in BPH-1 cells exposed to M. genitalium WT that could explain its ability to induce malignant transformation of benign cells. Validation of selected proteomic targets was carried out by Western blot analysis. Results: Both M. genitalium WT and Mg260_3 strains showed somewhat similar growth curve when absorbance at 450nm was matched at day 0. Colony forming units (CFUs) were similar for both strains at the same absorbance. However, the ability to infect BPH-1 cells was greatly reduced in Mg260_3 compared to the M. genitalium WT (p < 0.001). This was evident by infectivity assays and confocal microscopy. Proteomic analysis of BPH-1 cells infected with either M. genitalium WT or Mg260_3 for 8 hr, 24 hr and 6 days demonstrated a considerable shift in protein expression over uninfected BPH-1 cells at each time point. The preponderance of perturbed proteins regulated protein synthesis and protein processing, triggering endoplasmic reticulum stress. Conclusions: In summary, we demonstrate that Mg260_3, which is deficient of the phosphonate ABC transporter substrate-binding protein;MG289 (homologue to M. hyorhinis p37), is less effective in invading and maintaining an intracellular persistence in benign human prostate cells. In addition, deletion of MG289 resulted in altered BPH-1 responses to M. genitalium infection as evidenced by differential proteome profiling of BPH-1 infected cells.展开更多
Objective To investigate Mycoplasma genitalium (Mg) infection in high risk populations of sexually transmitted diseases (STDs) in China by means of culture, Mg DNA detection, observation under an electron microscope...Objective To investigate Mycoplasma genitalium (Mg) infection in high risk populations of sexually transmitted diseases (STDs) in China by means of culture, Mg DNA detection, observation under an electron microscope (EM) and an atomic force microscope (AFM) Methods 227 urogenital specimens taken from both patients in clinics and high risk populations of STDs in Beijing, Nanjing and Shanghai areas were cultured in SP 4 medium for isolation of Mg Suspicious positive strains were identified first by PCR technique which could amplify the special DNA fragments of Mg adhesion protein (Mg Pa) and 16S rRNA sequences The morphologic features of Mg were observed under EM and AFM Results Eight strains were isolated from the 227 specimens, the total positive rate was 3 52% The isolated strains met the criteria of growth and metabolic conditions required by Mg Specific fragments of Mg DNA (374bp based on Mg Pa and 402bp on 16S rRNA primers, respectively) could be amplified from both the isolates and the genital secretions Under EM and AFM, Mg showed different shapes, including flask, pear, spindle and globe shape The typical shape of Mg was flask or pear shape with projecting neck portion and broadened terminal structure under EM and with constrictive ring between the neck and body under AFM Conclusion Mg infection exists in high risk populations of STDs in China We highly suggest the use of AFM in dermatological study ?展开更多
To detect Mycoplasma genitalium (Mg) infection in high risk populations of sexually transmitted diseases (STDs) from several cities in China and to clarify the possible role of Mg in the pathogenesis of non ...To detect Mycoplasma genitalium (Mg) infection in high risk populations of sexually transmitted diseases (STDs) from several cities in China and to clarify the possible role of Mg in the pathogenesis of non gonococcal urethritis Methods Polymerase chain reaction (PCR) based on 2 pairs of primers, one for Mg Pa (adhesion protein) and the other for 16S rRNA (Mycoplasma genera) was used to detect Mg infection Urogenital specimens of different high risk populations and urethritis patients, were collected from the Guangdong, Kunming, Shanghai, Nanjing, and Changzhou areas Results The positive detection rate of Mg DNA in high risk populations of STDs was significantly higher than that of the control group (χ 2=7 82, P <0 01) The positive detection rates in Guangdong STD clinics and promiscuous persons from Kunming were higher than those from the Shanghai, Nanjing, and Changzhou areas (χ 2=8 54, P <0 01 and χ 2=5 89, P <0 05) Mg DNA could be detected in those patients without other relevant pathogens Some patients were simultaneously infected with Mg and other microbes, such as Chlamydia trachomatis and/or Ureaplasma urelyticum The positive Mg DNA detection rate in patients with urethritis symptoms was higher than in patients without the symptoms (χ 2=11 68, P <0 01) Conclusion Mg infection exists in high risk populations of STDs in China, and the Mg infection rate is different among different high risk groups展开更多
目的分析高危型人乳头瘤病毒(high-risk human papillomavirus,HR-HPV)感染的相关危险因素。方法选取2020年10月—2021年1月就诊于首都医科大学附属北京妇产医院妇科门诊的HR-HPV感染者作为感染组,同期于妇科门诊行子宫颈癌筛查且结果为...目的分析高危型人乳头瘤病毒(high-risk human papillomavirus,HR-HPV)感染的相关危险因素。方法选取2020年10月—2021年1月就诊于首都医科大学附属北京妇产医院妇科门诊的HR-HPV感染者作为感染组,同期于妇科门诊行子宫颈癌筛查且结果为HR-HPV阴性的患者作为对照组。两组均填写自行设计的《HR-HPV感染相关危险因素调查表》,统计分析HR-HPV感染相关危险因素。结果共纳入感染组患者125例,对照组患者53例。对两组进行单因素组间比较显示,感染组无业或职业社会经济地位较低、用洗剂清洁外阴、冲洗阴道频率高、性取向为同性、生育次数多、既往阴道炎病史、阴道分泌物量多、阴道分泌物性状异常和沙眼衣原体感染率均高于对照组(P均<0.05)。多因素Logistic回归分析显示,生育史(OR=5.106,95%CI:1.521~17.145,P=0.008)、既往阴道炎病史(OR=3.910,95%CI:1.167~13.099,P=0.027)、阴道分泌物异常(OR=758.313,95%CI:58.151~9888.714,P<0.001)是HR-HPV感染的危险因素。此外,用洗剂清洗外阴或冲洗阴道的清洁习惯(OR=2.004)、性取向为同性(OR=13.972)、沙眼衣原体阳性(OR=15.058)均显示出与HR-HPV感染具有较强的关联性,但由于对照组样本量较少,并未得出有统计学意义的结果。结论HR-HPV感染与多种因素相关,生育次数多、既往阴道炎病史、阴道分泌物性状异常是HR-HPV感染的危险因素,建议重视阴道分泌物变化,如有异常,及时就诊。在有条件的情况下,行宫颈HR-HPV筛查时加入阴道微生态的相关检查,如患有生殖道炎性疾病,应及时治疗,从多方面预防HR-HPV感染及降低HR-HPV持续感染的风险。展开更多
文摘AIMTo determine the association between chlamydial conjunctivitis and genital infection by Chlamydia trachomatis, Mycoplasma genitalium and Candida albicans, in addition to the possible relationship between cultured bacterial pathogens and oculogenital chlamydial infection.METHODSThis study was performed on 100 (50 symptomatic and 50 asymptomatic) women attending the Gynecological and Obstetric outpatient clinic of Alzahra hospital, Alazhar University. Simultaneously a conjunctival swab was taken from these patients. Polymerase chain reaction (PCR) was done on DNA extracted from both vaginal and conjunctival swab samples. Culture for both vaginal and conjunctival swabs was also done.RESULTSCandida albicans was the predominant organism isolated by culture in 20% and 40% of conjunctival and vaginal swabs respectively. By the PCR method, ocular Chlamydia trachomatis was present in 60% of symptomatic women, while genital Chlamydia trachomatis infection was present in 30% of symptomatic women. The results of this method also indicated that 25/50 (50%) vaginal swabs were positive with PCR for Candida albicans versus 15/50 (30%) were PCR positive in conjunctival swab. Mycoplasma genitalium was present in only 10% of vaginal swabs. Concomitant oculogenital PCR positive results for Chlamydia trachomatis and Candida albicans were 30% and 28% respectively.CONCLUSIONOcular Chlamydia trachomatis was associated with genital Chlamydia trachomatis in a high percentage of women followed by Candida albicans. Cultured bacterial organisms do not play a role in enhancement of Chlamydia trachomatis infection.
文摘Introduction: Recent studies suggest that infectious organisms may facilitate initiation and metastasis of many human cancers. One infectious organism of interest is Mycoplasma genitalium(Mg), a prevalent organism in humans known to cause sexually transmitted infection, as well as urethritis and prostatitis. Previous studies have demonstrated that benign, non-tumorigenic human prostate cells (BPH-1) chronically exposed to M. genitalium led to the malignant transformation of these cells as demonstrated in in vitro and in vivo models. Based on work from our laboratory, we felt this malignant transformation revolved around a specific M. genitalium’s ABC transporter (MG289) with homology to M. hyorhinis’ ABC transporter, p37. In this study, differences in M. genitalium’s ability to infect and induce a unique proteome conducive to tumoral growth were studied with engineered M. genitalium in which the p37 protein was silent. Materials and Methods: Wild-type M. genitalium (strain 431c, designated as M. genitalium WT) and MG289 deficient M. genitalium mutant (strain 260_3, designated as Mg260_3) were used for this study. We studied the infectivity potential between M. genitalium WT and Mg260_3 upon exposure to BPH-1 cells. Furthermore, we set out to identify a unique proteome in BPH-1 cells exposed to M. genitalium WT that could explain its ability to induce malignant transformation of benign cells. Validation of selected proteomic targets was carried out by Western blot analysis. Results: Both M. genitalium WT and Mg260_3 strains showed somewhat similar growth curve when absorbance at 450nm was matched at day 0. Colony forming units (CFUs) were similar for both strains at the same absorbance. However, the ability to infect BPH-1 cells was greatly reduced in Mg260_3 compared to the M. genitalium WT (p < 0.001). This was evident by infectivity assays and confocal microscopy. Proteomic analysis of BPH-1 cells infected with either M. genitalium WT or Mg260_3 for 8 hr, 24 hr and 6 days demonstrated a considerable shift in protein expression over uninfected BPH-1 cells at each time point. The preponderance of perturbed proteins regulated protein synthesis and protein processing, triggering endoplasmic reticulum stress. Conclusions: In summary, we demonstrate that Mg260_3, which is deficient of the phosphonate ABC transporter substrate-binding protein;MG289 (homologue to M. hyorhinis p37), is less effective in invading and maintaining an intracellular persistence in benign human prostate cells. In addition, deletion of MG289 resulted in altered BPH-1 responses to M. genitalium infection as evidenced by differential proteome profiling of BPH-1 infected cells.
文摘Objective To investigate Mycoplasma genitalium (Mg) infection in high risk populations of sexually transmitted diseases (STDs) in China by means of culture, Mg DNA detection, observation under an electron microscope (EM) and an atomic force microscope (AFM) Methods 227 urogenital specimens taken from both patients in clinics and high risk populations of STDs in Beijing, Nanjing and Shanghai areas were cultured in SP 4 medium for isolation of Mg Suspicious positive strains were identified first by PCR technique which could amplify the special DNA fragments of Mg adhesion protein (Mg Pa) and 16S rRNA sequences The morphologic features of Mg were observed under EM and AFM Results Eight strains were isolated from the 227 specimens, the total positive rate was 3 52% The isolated strains met the criteria of growth and metabolic conditions required by Mg Specific fragments of Mg DNA (374bp based on Mg Pa and 402bp on 16S rRNA primers, respectively) could be amplified from both the isolates and the genital secretions Under EM and AFM, Mg showed different shapes, including flask, pear, spindle and globe shape The typical shape of Mg was flask or pear shape with projecting neck portion and broadened terminal structure under EM and with constrictive ring between the neck and body under AFM Conclusion Mg infection exists in high risk populations of STDs in China We highly suggest the use of AFM in dermatological study ?
文摘To detect Mycoplasma genitalium (Mg) infection in high risk populations of sexually transmitted diseases (STDs) from several cities in China and to clarify the possible role of Mg in the pathogenesis of non gonococcal urethritis Methods Polymerase chain reaction (PCR) based on 2 pairs of primers, one for Mg Pa (adhesion protein) and the other for 16S rRNA (Mycoplasma genera) was used to detect Mg infection Urogenital specimens of different high risk populations and urethritis patients, were collected from the Guangdong, Kunming, Shanghai, Nanjing, and Changzhou areas Results The positive detection rate of Mg DNA in high risk populations of STDs was significantly higher than that of the control group (χ 2=7 82, P <0 01) The positive detection rates in Guangdong STD clinics and promiscuous persons from Kunming were higher than those from the Shanghai, Nanjing, and Changzhou areas (χ 2=8 54, P <0 01 and χ 2=5 89, P <0 05) Mg DNA could be detected in those patients without other relevant pathogens Some patients were simultaneously infected with Mg and other microbes, such as Chlamydia trachomatis and/or Ureaplasma urelyticum The positive Mg DNA detection rate in patients with urethritis symptoms was higher than in patients without the symptoms (χ 2=11 68, P <0 01) Conclusion Mg infection exists in high risk populations of STDs in China, and the Mg infection rate is different among different high risk groups
文摘目的分析高危型人乳头瘤病毒(high-risk human papillomavirus,HR-HPV)感染的相关危险因素。方法选取2020年10月—2021年1月就诊于首都医科大学附属北京妇产医院妇科门诊的HR-HPV感染者作为感染组,同期于妇科门诊行子宫颈癌筛查且结果为HR-HPV阴性的患者作为对照组。两组均填写自行设计的《HR-HPV感染相关危险因素调查表》,统计分析HR-HPV感染相关危险因素。结果共纳入感染组患者125例,对照组患者53例。对两组进行单因素组间比较显示,感染组无业或职业社会经济地位较低、用洗剂清洁外阴、冲洗阴道频率高、性取向为同性、生育次数多、既往阴道炎病史、阴道分泌物量多、阴道分泌物性状异常和沙眼衣原体感染率均高于对照组(P均<0.05)。多因素Logistic回归分析显示,生育史(OR=5.106,95%CI:1.521~17.145,P=0.008)、既往阴道炎病史(OR=3.910,95%CI:1.167~13.099,P=0.027)、阴道分泌物异常(OR=758.313,95%CI:58.151~9888.714,P<0.001)是HR-HPV感染的危险因素。此外,用洗剂清洗外阴或冲洗阴道的清洁习惯(OR=2.004)、性取向为同性(OR=13.972)、沙眼衣原体阳性(OR=15.058)均显示出与HR-HPV感染具有较强的关联性,但由于对照组样本量较少,并未得出有统计学意义的结果。结论HR-HPV感染与多种因素相关,生育次数多、既往阴道炎病史、阴道分泌物性状异常是HR-HPV感染的危险因素,建议重视阴道分泌物变化,如有异常,及时就诊。在有条件的情况下,行宫颈HR-HPV筛查时加入阴道微生态的相关检查,如患有生殖道炎性疾病,应及时治疗,从多方面预防HR-HPV感染及降低HR-HPV持续感染的风险。
