The goal of the study was to quantify the genetic diversity of different potato varieties. Five groups were named to classify the 25 genres of potatoes as: Cluster I (Lalpakri, Diamond), Cluster II (Hagrai, Green Moun...The goal of the study was to quantify the genetic diversity of different potato varieties. Five groups were named to classify the 25 genres of potatoes as: Cluster I (Lalpakri, Diamond), Cluster II (Hagrai, Green Mountain, G.M.O., Elvera), Cluster III (Atlantic, Shepody, Raja, Fundy, Multa, Granulla, Shibilati, Japanese Red), Cluster IV (Atlas, Brondy, Yucon Gold, Monona, Petrones, Cheroki), and Cluster V (Calwhite, Prelude, Allblue, Russet, Burbank, TPS-67). The highest and lowest genotypes are found in Clusters III and I, respectively. The two groups’ maximum inter-cluster distance (the cluster’s distance between them) demonstrates the enormous diversity between Clusters II & III. Cluster III had the greatest intra-cluster distance (distance within a set), whereas Cluster V had the smallest. In most cases, the distance between gaps was more significant than the distance within the holes, showing greater genetic diversity between different groups’ genotypes. The highest, second highest, and third highest eigenvalues, accompanying the positive canonical values for Vectors I and II of three characteristics: average tuber weight in each plant, the tubers number in each plant, and the eyes number in each tuber, showed the most outstanding contribution to the complete difference between genotypes. Of the nine features, the smaller leaves number in each plant, the shorter plant height, the more minor genotypes, the fewer eyes per tube generated from Cluster I, the maximum height of the plants, the high-quality tubers in each plant, the tubers number in each plant, the fresh weight in each plant, and the leaf number in each plant from Cluster II could be chosen in the role of parents in this program for hybridization. Given the size of the genetic distance, the various characteristics that contribute to the overall difference, and the average population size, Hagrai, Green Mountain, O.M.G., Elvera Cluster II and Cluster I, Lalpakri, and Diamond genotypes can be regarded as parents of hybridization programs in the future. Thus, producers can get guidance to enhance genetic diversity by selecting materials from different relatives and reducing their vulnerability to diseases and climate change.展开更多
AIM: To assess vitamin D (Vit D) abnormalities in hepatitis C infected patients and their relationship with interleukin (IL)-17, IL-23 and N-terminal propeptide of type Ⅲ pro-collagen (PⅢNP) as immune response media...AIM: To assess vitamin D (Vit D) abnormalities in hepatitis C infected patients and their relationship with interleukin (IL)-17, IL-23 and N-terminal propeptide of type Ⅲ pro-collagen (PⅢNP) as immune response mediators. METHODS: The study was conducted on 50 Egyptian patients (36 male, 14 female) with hepatitis C virus (HCV) infection, who visited the Hepatology Outpatient Clinic in the Endemic Disease Hospital at Cairo University. Patients were compared with 25 ageand sexmatched healthy individuals. Inclusion criteria were based on a history of liver disease with HCV genotype 4 (HCV-4) infection (as new patients or under followup). Based on ultrasonography, patients were classified into four subgroups; 14 with bright hepatomegaly; 11 with perihepatic fibrosis; 11 with hepatic cirrhosis; and 14 with cirrhosis and hepatocellular carcinoma (HCC).Total Vit D (i.e., 25-OH-Vit D) and active Vit D [i.e., 1,25-(OH) 2 -Vit D] assays were carried out using commercial kits. IL-17, IL-23 and PⅢNP levels were assayed using enzyme linked immunosorbent assay kits, while HCV virus was measured by quantitative and qualitative polymerase chain reaction. RESULTS: Levels of Vit D and its active form were significantly lower in advanced liver disease (hepatic cirrhosis and/or carcinoma) patients, compared to those with bright hepatomegaly and perihepatic fibrosis. IL-17, IL-23 and PⅢNP levels were markedly increased in HCV patients and correlated with the progression of hepatic damage. The decrease in Vit D and active Vit D was concomitant with an increase in viral load, as well as levels of IL-17, IL-23 and PⅢNP among all subgroups of HCV-infected patients, compared to normal healthy controls. A significant negative correlation was evident between active Vit D and each of IL-17, IL-23 and PⅢNP (r = -0.679, -0.801 and -0.920 at P < 0.001, respectively). HCV-infected men and women showed no differences with respect to Vit D levels. The viral load was negatively correlated with Vit D and active Vit D (r = -0.084 and -0.846 at P < 0.001, respectively), and positively correlated with IL-17, IL-23 and PⅢNP (r = 0.951, 0.922 and 0.94 at P < 0.001, respectively). Whether the deficiency in Vit D was related to HCVinduced chronic liver disease or was a predisposing factor for a higher viral load remains to be elucidated. CONCLUSION: The negative correlations between Vit D and IL-17, IL-23 and PⅢNP highlight their involvement in the immune response in patients with HCV-4related liver diseases in Egypt.展开更多
Objective:To examine the multiplication efficiency Japanese encephalitis virus(JEV)genotype Ⅰ(G Ⅰ) and genotype Ⅲ(GⅢ) of different cell lines which originated from human,porcine,mosquitoes in order to prove mechan...Objective:To examine the multiplication efficiency Japanese encephalitis virus(JEV)genotype Ⅰ(G Ⅰ) and genotype Ⅲ(GⅢ) of different cell lines which originated from human,porcine,mosquitoes in order to prove mechanism of JEV G Ⅰ replacement JEV GⅢ since it emerging in nature recent decades.Methods:The mixture of Gi and GⅢ JEV isolates was inoculated on human rhabdomyosarcoma(RD).pig kidney epithelial(PS) and Aedes albopictus C6/36 clone(C6/36) which originated from human,porcine and mosquitoes,respectively.Plaque assays were performed to calculate virus titer and real-time RT-PCR with GⅠand GⅢspecific primer sets to quantify the number of GⅠ and GUI RNA copies.Results:The highest virus titer reached at the 3rd day of post infection when G Ⅰand GⅢ mixture was inoculated on RD and PS and that of C636 was at the 4^(th)day.JEVs were amplified and maintained by C6/36 cells after 10 passages whereas that by RD and PS only limited within 8 and 6 passages,respectively.GⅠ strain amplified and maintained more efficiently on C6/36 and PS but not RD.whereas GⅢ strain amplified and maintained more efficiently on RD.Conclusions:There is a correlation between the multiplication efficiency of GⅠ and GⅢ JEV strains when these two genotype strains co-infected on different cell lines with the predominance of GⅠstrains in C6/36 and PS and the limited detection of G 1 strains in RD cells proving a possible mechanism of shift JEV genotypes in nature recent decades since GⅠ emerging.展开更多
Background:Salt stress significantly inhibits the growth,development,and productivity of cotton because of osmotic,ionic,and oxidative stresses.Therefore,the screening and development of salt tolerant cotton cultivars...Background:Salt stress significantly inhibits the growth,development,and productivity of cotton because of osmotic,ionic,and oxidative stresses.Therefore,the screening and development of salt tolerant cotton cultivars is a key issue towards sustainable agriculture.This study subjected 11 upland cotton genotypes at the seedling growth stage to five different salt concentrations and evaluated their salt tolerance and reliable traits.Results:Several morpho-physiological traits were measured after 10 days of salinity treatment and the salt tolerance performance varied significantly among the tested cotton genotypes.The optimal Na Cl concentration for the evaluation of salt tolerance was 200 mmol·L-1.Membership function value and salt tolerance index were used to identify the most consistent salt tolerance traits.Leaf relative water content and photosynthesis were identified as reliable indicators for salt tolerance at the seedling stage.All considered traits related to salt tolerance indices were significantly and positively correlated with each other except for malondialdehyde.Cluster heat map analysis based on the morpho-physiological salt tolerance-indices clearly discriminated the 11 cotton genotypes into three different salt tolerance clusters.Cluster I represented the salt-tolerant genotypes(Z9807,Z0228,and Z7526)whereas clusters II(Z0710,Z7514,Z1910,and Z7516)and III(Z0102,Z7780,Z9648,and Z9612)represented moderately salttolerant and salt-sensitive genotypes,respectively.Conclusions:A hydroponic screening system was established.Leaf relative water content and photosynthesis were identified as two reliable traits that adequately represented the salt tolerance of cotton genotypes at the seedling growth stage.Furthermore,three salt-tolerant genotypes were identified,which might be used as genetic resources for the salt-tolerance breeding of cotton.展开更多
The research work was undertaken to identify the extent of genetic diversity in different parameters of wheat related to heat tolerance mechanism. Performances of currently available 25 spring wheat genotypes were stu...The research work was undertaken to identify the extent of genetic diversity in different parameters of wheat related to heat tolerance mechanism. Performances of currently available 25 spring wheat genotypes were studied at the Regional Wheat Research Institute, Shyampur, Rajshahi during the winter season of 2016/2017. All these genotypes (25) were grouped into five clusters on the basis of non-hierarchical clustering parameters viz. cluster I (G2, G5, G6, G16, G20), II (G4, G7, G9, G11, G12, G17), III (G10, G24), IV (G1, G13, G19, G21) and V (G3, G8, G14, G15, G18, G22, G23, G25). These groups were arranged into five (5) pairs of clusters viz. cluster I and III;II and III;II and V;III and IV;IV and V considering their similar potentiality of different traits. Results revealed that the maximum number of genotypes (8) was found in cluster V while cluster III comprised minimum genotypes (2). The inter-cluster distance was higher than intra-cluster distances. The highest and second highest eigenvalues belonged to spike/m<sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> (25.23%) and spikelets/spike (20.18%) respectively, along with positive canonical values in both the vectors 1 and 2 for these two traits identified them as major traits by exposing their highest potential toward genetic divergence. Cluster III produced the highest spike/m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> (388.8), grain/spike (48.1), plant height (89.5 cm), SPAD (54.2), yield (2799 kg/ha) and biomass (7758.1 Kg/ha) with lowest heading days (64.7 days), maturity days (98.7 days), canopy temperature at vegetative stage (21.7°</span><span style="font-family:Verdana;">C</span><span style="font-family:Verdana;">) and canopy temperature at grain filling stage (22.4</span><span style="font-family:Verdana;">°</span><span style="font-family:Verdana;">C</span><span style="font-family:Verdana;">). In contrast, cluster V produced the lowest 1000-grain weight (30.4 g</span><span style="font-family:Verdana;">) and grain yield (2172 kg/ha) belonging to the highest canopy temperature at both stages (23.8°</span><span style="font-family:Verdana;">C</span><span style="font-family:Verdana;"> and 24.7</span><span style="font-family:Verdana;">°</span><span style="font-family:Verdana;">C</span><span style="font-family:Verdana;">). </span><span style="font-family:;" "=""><span style="font-family:Verdana;">However, results suggested that the genotypes G10 and G24 under the cluster III, and genotypes G01, G13, G19 and G21 under the cluster IV could be considered as parents for future hybridization program, as well as the five pairs of clusters viz. cluster I and III;II and III;II and V;III and IV;IV and V might be matched as considered for getting more heterotic F</span><sub><span style="font-family:Verdana;">1</span></sub><span style="font-family:Verdana;">. The results of the study would help to identify the divergent genotypes associate with heat tolerance and this might be helpful in designing future breeding program.</span></span>展开更多
Information about the amount and distribution of genetic variation in germplasm collections is important for their efficient management and effective utilization in plant breeding. Therefore this study was conducted t...Information about the amount and distribution of genetic variation in germplasm collections is important for their efficient management and effective utilization in plant breeding. Therefore this study was conducted to assess genetic diversity of sugarcane germplasm in Ethiopia. An experiment comprising of 400 sugarcane genotypes (174 local and 226 introduced) was conducted between March 2012 and October 2013 at Wonji and Metehara Sugar Estates using partial balanced lattice design with two replications. Data was recorded on 21 quantitative characters which included cane yield and its components, sugar yield and sugar quality traits. ANOVA portrayed highly significant differences (P < 0.01) among the genotypes for 21 quantitative traits. Cluster analysis revealed intra cluster D<sup>2 </sup>values ranging from 2.16 - 10.60 and inter cluster from 7.24 - 5864. There were six principal components accounting for 79.26% of the total variation in the tested materials. Millable stalk count, single cane weight, stalk diameter, cane yield, sugar yield and sugar quality traits showed high positive loading on the first two PCs and accounted for most of the variation observed among the genotypes. Therefore, this study suggested that the important characters responsible for diversity in the sugarcane genotypes could be grouped in two principal components namely “Yield” and “Quality” with “Yield” traits being comparatively more important than “Quality”. Genotypes clustered for high mean values of various traits could be exploited for further improvement of the crop either through selection or through hybridization. The clusters having high mean value for yield could be selected for yield per se as well.展开更多
为了从产量、品质方面准确评价旱肥地试验小麦新品系,以2020—2021年连续2年参加国家黄淮冬麦区旱地组多点比较试验的26份新品系为材料,应用多元统计方法分析了大田条件下2年间产量和品质性状的变化。结果表明:26份品系的产量和品质性状...为了从产量、品质方面准确评价旱肥地试验小麦新品系,以2020—2021年连续2年参加国家黄淮冬麦区旱地组多点比较试验的26份新品系为材料,应用多元统计方法分析了大田条件下2年间产量和品质性状的变化。结果表明:26份品系的产量和品质性状2年变异系数分别为2.0%~74.2%和2.1%~95.1%,变异较大,变异系数大小顺序依次为稳定时间>湿面筋含量>蛋白质含量>吸水率=单位面积产量>容重;相关分析表明,2年产量与品质性状存在负相关关系,稳定时间均与蛋白质含量、吸水量正相关,稳定时间与产量负相关,其中稳定时间与蛋白质含量相关系数均较高。在相关分析的基础上,采用聚类分析方法将2年中26份小麦参试品系聚为4类,并在主成分品种、性状(genotype by trait,GT)双标图(biplot)和聚类图中进行展示,聚类结果与新品系的实际表现一致,其中‘泰科麦4835’‘洛旱35’‘农大162’‘山农611436’连续2年划为同一类型,表现为产量较高、品质优良。该研究结果可为参试新品系的合理评价和推广应用提供理论依据。展开更多
Twenty-four rice genotypes were examined to assess genetic variability,heritability,and correlations for seven-grain quality traits,eight nutritional elements,and protein.ANOVA revealed significant differences for the ...Twenty-four rice genotypes were examined to assess genetic variability,heritability,and correlations for seven-grain quality traits,eight nutritional elements,and protein.ANOVA revealed significant differences for the quality traits studied.For every trait under study,the phenotypic coefficient of variation was higher than the correspon-dence genotypic coefficient of variation.Heritability in a broad sense varied from 29.75%for grain length to 98.31%for the elongation trait.Hulling percentage recovery had a highly significant positive correlation with milling and head rice percentage.Consequently,milling percentage had a highly positive correlation with head rice percentage.In amylose percentage,all the genotypes belonged to low amylose except the Hassawi-1 variety,which had intermediate amylose content.Mineral nutrition contents of magnesium(Mg),sodium(Na),potas-sium(K),calcium(Ca),copper(Cu),manganese(Mn),zinc(Zn),iron(Fe),or protein percentage gave different variations for 24 rice genotypes under all the nutritional elements.Among the 24 genotypes,ten rice genotypes–HighNutrient-1,HighNutrient-2,HighNutrient-9,HighNutrient-8,HighNutrient-3,Hassawi-2,HighNutrient-7,HighNutrient-6,Hassawi-1,and HighNutrient-4–had the highest heist value for all nutritional and protein con-tents,and could be used as a donor to improving new varieties.There was a positive and significant correlation between magnesium Mg,K,Zn and Fe.Consequently,K had a positive correlation with zinc Zn,Fe,and protein percentage.Clustering analysis was divided into two groups:thefirst group included all genotypes rich in nutri-ents,while the remaining genotypes with low nutritional content were included in the second group.展开更多
Taking 65 cherry tomato core germplasms as experimental materials,the genetic diversity of seven agronomic traits were analyzed.The correlation between any two of the seven agronomic traits and the genetic relationshi...Taking 65 cherry tomato core germplasms as experimental materials,the genetic diversity of seven agronomic traits were analyzed.The correlation between any two of the seven agronomic traits and the genetic relationships of these germplasms were analyzed based on genotypic values.The genetic diversity indices of the seven agronomic traits were 4.15,4.13,4.16,4.13,4.13,4.13 and 4.01,respectively,showing that the cherry tomato core collection had abundant genetic diversity.The correlation analysis between traits based on genotype effect values showed that leaf length was significantly correlated with leaf width with the correlation coefficient of 0.56.The fruit width was significantly correlated with fruit length with the correlation coefficient of 0.52.The flesh thickness was significantly correlated with fruit length and fruit width with the correlation coefficients of 0.49 and 0.39,respectively.The single fruit weight was significantly correlated with fruit length,fruit width and flesh thickness with the correlation coefficients of 0.44,0.61 and 0.62,respectively.When the genetic distances between core germplasms of cherry tomato were calculated based on the phenotypic values,65 core germplasms of cherry tomato were divided into three groups with the rescaled distance of 10.When the genetic distances between core germplasms of cherry tomato were calculated based on the genotypic values,the 65 core germplasms of cherry tomato were divided into four groups with the rescaled distance of 10.Comparing the 2 clustering results,it could find that genotypic value-based clustering analysis could better clarify the genetic relationship between core germplasms.This study could provide a theoretical basis for the effective utilization of cherry tomato core germplasms.展开更多
文摘The goal of the study was to quantify the genetic diversity of different potato varieties. Five groups were named to classify the 25 genres of potatoes as: Cluster I (Lalpakri, Diamond), Cluster II (Hagrai, Green Mountain, G.M.O., Elvera), Cluster III (Atlantic, Shepody, Raja, Fundy, Multa, Granulla, Shibilati, Japanese Red), Cluster IV (Atlas, Brondy, Yucon Gold, Monona, Petrones, Cheroki), and Cluster V (Calwhite, Prelude, Allblue, Russet, Burbank, TPS-67). The highest and lowest genotypes are found in Clusters III and I, respectively. The two groups’ maximum inter-cluster distance (the cluster’s distance between them) demonstrates the enormous diversity between Clusters II & III. Cluster III had the greatest intra-cluster distance (distance within a set), whereas Cluster V had the smallest. In most cases, the distance between gaps was more significant than the distance within the holes, showing greater genetic diversity between different groups’ genotypes. The highest, second highest, and third highest eigenvalues, accompanying the positive canonical values for Vectors I and II of three characteristics: average tuber weight in each plant, the tubers number in each plant, and the eyes number in each tuber, showed the most outstanding contribution to the complete difference between genotypes. Of the nine features, the smaller leaves number in each plant, the shorter plant height, the more minor genotypes, the fewer eyes per tube generated from Cluster I, the maximum height of the plants, the high-quality tubers in each plant, the tubers number in each plant, the fresh weight in each plant, and the leaf number in each plant from Cluster II could be chosen in the role of parents in this program for hybridization. Given the size of the genetic distance, the various characteristics that contribute to the overall difference, and the average population size, Hagrai, Green Mountain, O.M.G., Elvera Cluster II and Cluster I, Lalpakri, and Diamond genotypes can be regarded as parents of hybridization programs in the future. Thus, producers can get guidance to enhance genetic diversity by selecting materials from different relatives and reducing their vulnerability to diseases and climate change.
文摘AIM: To assess vitamin D (Vit D) abnormalities in hepatitis C infected patients and their relationship with interleukin (IL)-17, IL-23 and N-terminal propeptide of type Ⅲ pro-collagen (PⅢNP) as immune response mediators. METHODS: The study was conducted on 50 Egyptian patients (36 male, 14 female) with hepatitis C virus (HCV) infection, who visited the Hepatology Outpatient Clinic in the Endemic Disease Hospital at Cairo University. Patients were compared with 25 ageand sexmatched healthy individuals. Inclusion criteria were based on a history of liver disease with HCV genotype 4 (HCV-4) infection (as new patients or under followup). Based on ultrasonography, patients were classified into four subgroups; 14 with bright hepatomegaly; 11 with perihepatic fibrosis; 11 with hepatic cirrhosis; and 14 with cirrhosis and hepatocellular carcinoma (HCC).Total Vit D (i.e., 25-OH-Vit D) and active Vit D [i.e., 1,25-(OH) 2 -Vit D] assays were carried out using commercial kits. IL-17, IL-23 and PⅢNP levels were assayed using enzyme linked immunosorbent assay kits, while HCV virus was measured by quantitative and qualitative polymerase chain reaction. RESULTS: Levels of Vit D and its active form were significantly lower in advanced liver disease (hepatic cirrhosis and/or carcinoma) patients, compared to those with bright hepatomegaly and perihepatic fibrosis. IL-17, IL-23 and PⅢNP levels were markedly increased in HCV patients and correlated with the progression of hepatic damage. The decrease in Vit D and active Vit D was concomitant with an increase in viral load, as well as levels of IL-17, IL-23 and PⅢNP among all subgroups of HCV-infected patients, compared to normal healthy controls. A significant negative correlation was evident between active Vit D and each of IL-17, IL-23 and PⅢNP (r = -0.679, -0.801 and -0.920 at P < 0.001, respectively). HCV-infected men and women showed no differences with respect to Vit D levels. The viral load was negatively correlated with Vit D and active Vit D (r = -0.084 and -0.846 at P < 0.001, respectively), and positively correlated with IL-17, IL-23 and PⅢNP (r = 0.951, 0.922 and 0.94 at P < 0.001, respectively). Whether the deficiency in Vit D was related to HCVinduced chronic liver disease or was a predisposing factor for a higher viral load remains to be elucidated. CONCLUSION: The negative correlations between Vit D and IL-17, IL-23 and PⅢNP highlight their involvement in the immune response in patients with HCV-4related liver diseases in Egypt.
基金funded by Vietnam National Foundation for ScienceTechnology Development(NAFOSTED)under grant number:106.16-2011.68
文摘Objective:To examine the multiplication efficiency Japanese encephalitis virus(JEV)genotype Ⅰ(G Ⅰ) and genotype Ⅲ(GⅢ) of different cell lines which originated from human,porcine,mosquitoes in order to prove mechanism of JEV G Ⅰ replacement JEV GⅢ since it emerging in nature recent decades.Methods:The mixture of Gi and GⅢ JEV isolates was inoculated on human rhabdomyosarcoma(RD).pig kidney epithelial(PS) and Aedes albopictus C6/36 clone(C6/36) which originated from human,porcine and mosquitoes,respectively.Plaque assays were performed to calculate virus titer and real-time RT-PCR with GⅠand GⅢspecific primer sets to quantify the number of GⅠ and GUI RNA copies.Results:The highest virus titer reached at the 3rd day of post infection when G Ⅰand GⅢ mixture was inoculated on RD and PS and that of C636 was at the 4^(th)day.JEVs were amplified and maintained by C6/36 cells after 10 passages whereas that by RD and PS only limited within 8 and 6 passages,respectively.GⅠ strain amplified and maintained more efficiently on C6/36 and PS but not RD.whereas GⅢ strain amplified and maintained more efficiently on RD.Conclusions:There is a correlation between the multiplication efficiency of GⅠ and GⅢ JEV strains when these two genotype strains co-infected on different cell lines with the predominance of GⅠstrains in C6/36 and PS and the limited detection of G 1 strains in RD cells proving a possible mechanism of shift JEV genotypes in nature recent decades since GⅠ emerging.
基金supported by National Key R&D Program(2017YFD0101600)State Key Laboratory of Cotton Biology(CB2019C17)。
文摘Background:Salt stress significantly inhibits the growth,development,and productivity of cotton because of osmotic,ionic,and oxidative stresses.Therefore,the screening and development of salt tolerant cotton cultivars is a key issue towards sustainable agriculture.This study subjected 11 upland cotton genotypes at the seedling growth stage to five different salt concentrations and evaluated their salt tolerance and reliable traits.Results:Several morpho-physiological traits were measured after 10 days of salinity treatment and the salt tolerance performance varied significantly among the tested cotton genotypes.The optimal Na Cl concentration for the evaluation of salt tolerance was 200 mmol·L-1.Membership function value and salt tolerance index were used to identify the most consistent salt tolerance traits.Leaf relative water content and photosynthesis were identified as reliable indicators for salt tolerance at the seedling stage.All considered traits related to salt tolerance indices were significantly and positively correlated with each other except for malondialdehyde.Cluster heat map analysis based on the morpho-physiological salt tolerance-indices clearly discriminated the 11 cotton genotypes into three different salt tolerance clusters.Cluster I represented the salt-tolerant genotypes(Z9807,Z0228,and Z7526)whereas clusters II(Z0710,Z7514,Z1910,and Z7516)and III(Z0102,Z7780,Z9648,and Z9612)represented moderately salttolerant and salt-sensitive genotypes,respectively.Conclusions:A hydroponic screening system was established.Leaf relative water content and photosynthesis were identified as two reliable traits that adequately represented the salt tolerance of cotton genotypes at the seedling growth stage.Furthermore,three salt-tolerant genotypes were identified,which might be used as genetic resources for the salt-tolerance breeding of cotton.
文摘The research work was undertaken to identify the extent of genetic diversity in different parameters of wheat related to heat tolerance mechanism. Performances of currently available 25 spring wheat genotypes were studied at the Regional Wheat Research Institute, Shyampur, Rajshahi during the winter season of 2016/2017. All these genotypes (25) were grouped into five clusters on the basis of non-hierarchical clustering parameters viz. cluster I (G2, G5, G6, G16, G20), II (G4, G7, G9, G11, G12, G17), III (G10, G24), IV (G1, G13, G19, G21) and V (G3, G8, G14, G15, G18, G22, G23, G25). These groups were arranged into five (5) pairs of clusters viz. cluster I and III;II and III;II and V;III and IV;IV and V considering their similar potentiality of different traits. Results revealed that the maximum number of genotypes (8) was found in cluster V while cluster III comprised minimum genotypes (2). The inter-cluster distance was higher than intra-cluster distances. The highest and second highest eigenvalues belonged to spike/m<sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> (25.23%) and spikelets/spike (20.18%) respectively, along with positive canonical values in both the vectors 1 and 2 for these two traits identified them as major traits by exposing their highest potential toward genetic divergence. Cluster III produced the highest spike/m</span><sup><span style="font-family:Verdana;">2</span></sup><span style="font-family:Verdana;"> (388.8), grain/spike (48.1), plant height (89.5 cm), SPAD (54.2), yield (2799 kg/ha) and biomass (7758.1 Kg/ha) with lowest heading days (64.7 days), maturity days (98.7 days), canopy temperature at vegetative stage (21.7°</span><span style="font-family:Verdana;">C</span><span style="font-family:Verdana;">) and canopy temperature at grain filling stage (22.4</span><span style="font-family:Verdana;">°</span><span style="font-family:Verdana;">C</span><span style="font-family:Verdana;">). In contrast, cluster V produced the lowest 1000-grain weight (30.4 g</span><span style="font-family:Verdana;">) and grain yield (2172 kg/ha) belonging to the highest canopy temperature at both stages (23.8°</span><span style="font-family:Verdana;">C</span><span style="font-family:Verdana;"> and 24.7</span><span style="font-family:Verdana;">°</span><span style="font-family:Verdana;">C</span><span style="font-family:Verdana;">). </span><span style="font-family:;" "=""><span style="font-family:Verdana;">However, results suggested that the genotypes G10 and G24 under the cluster III, and genotypes G01, G13, G19 and G21 under the cluster IV could be considered as parents for future hybridization program, as well as the five pairs of clusters viz. cluster I and III;II and III;II and V;III and IV;IV and V might be matched as considered for getting more heterotic F</span><sub><span style="font-family:Verdana;">1</span></sub><span style="font-family:Verdana;">. The results of the study would help to identify the divergent genotypes associate with heat tolerance and this might be helpful in designing future breeding program.</span></span>
文摘Information about the amount and distribution of genetic variation in germplasm collections is important for their efficient management and effective utilization in plant breeding. Therefore this study was conducted to assess genetic diversity of sugarcane germplasm in Ethiopia. An experiment comprising of 400 sugarcane genotypes (174 local and 226 introduced) was conducted between March 2012 and October 2013 at Wonji and Metehara Sugar Estates using partial balanced lattice design with two replications. Data was recorded on 21 quantitative characters which included cane yield and its components, sugar yield and sugar quality traits. ANOVA portrayed highly significant differences (P < 0.01) among the genotypes for 21 quantitative traits. Cluster analysis revealed intra cluster D<sup>2 </sup>values ranging from 2.16 - 10.60 and inter cluster from 7.24 - 5864. There were six principal components accounting for 79.26% of the total variation in the tested materials. Millable stalk count, single cane weight, stalk diameter, cane yield, sugar yield and sugar quality traits showed high positive loading on the first two PCs and accounted for most of the variation observed among the genotypes. Therefore, this study suggested that the important characters responsible for diversity in the sugarcane genotypes could be grouped in two principal components namely “Yield” and “Quality” with “Yield” traits being comparatively more important than “Quality”. Genotypes clustered for high mean values of various traits could be exploited for further improvement of the crop either through selection or through hybridization. The clusters having high mean value for yield could be selected for yield per se as well.
文摘为了从产量、品质方面准确评价旱肥地试验小麦新品系,以2020—2021年连续2年参加国家黄淮冬麦区旱地组多点比较试验的26份新品系为材料,应用多元统计方法分析了大田条件下2年间产量和品质性状的变化。结果表明:26份品系的产量和品质性状2年变异系数分别为2.0%~74.2%和2.1%~95.1%,变异较大,变异系数大小顺序依次为稳定时间>湿面筋含量>蛋白质含量>吸水率=单位面积产量>容重;相关分析表明,2年产量与品质性状存在负相关关系,稳定时间均与蛋白质含量、吸水量正相关,稳定时间与产量负相关,其中稳定时间与蛋白质含量相关系数均较高。在相关分析的基础上,采用聚类分析方法将2年中26份小麦参试品系聚为4类,并在主成分品种、性状(genotype by trait,GT)双标图(biplot)和聚类图中进行展示,聚类结果与新品系的实际表现一致,其中‘泰科麦4835’‘洛旱35’‘农大162’‘山农611436’连续2年划为同一类型,表现为产量较高、品质优良。该研究结果可为参试新品系的合理评价和推广应用提供理论依据。
基金supported and funded by Deanship of Scientific Research,Vice Presidency for Graduate Studies and Scientific Research,King Faisal University,Saudi Arabia,grant number(Grant A410).
文摘Twenty-four rice genotypes were examined to assess genetic variability,heritability,and correlations for seven-grain quality traits,eight nutritional elements,and protein.ANOVA revealed significant differences for the quality traits studied.For every trait under study,the phenotypic coefficient of variation was higher than the correspon-dence genotypic coefficient of variation.Heritability in a broad sense varied from 29.75%for grain length to 98.31%for the elongation trait.Hulling percentage recovery had a highly significant positive correlation with milling and head rice percentage.Consequently,milling percentage had a highly positive correlation with head rice percentage.In amylose percentage,all the genotypes belonged to low amylose except the Hassawi-1 variety,which had intermediate amylose content.Mineral nutrition contents of magnesium(Mg),sodium(Na),potas-sium(K),calcium(Ca),copper(Cu),manganese(Mn),zinc(Zn),iron(Fe),or protein percentage gave different variations for 24 rice genotypes under all the nutritional elements.Among the 24 genotypes,ten rice genotypes–HighNutrient-1,HighNutrient-2,HighNutrient-9,HighNutrient-8,HighNutrient-3,Hassawi-2,HighNutrient-7,HighNutrient-6,Hassawi-1,and HighNutrient-4–had the highest heist value for all nutritional and protein con-tents,and could be used as a donor to improving new varieties.There was a positive and significant correlation between magnesium Mg,K,Zn and Fe.Consequently,K had a positive correlation with zinc Zn,Fe,and protein percentage.Clustering analysis was divided into two groups:thefirst group included all genotypes rich in nutri-ents,while the remaining genotypes with low nutritional content were included in the second group.
基金Supported by Hainan Provincial Science and Technology Project(ZDYF2018035)
文摘Taking 65 cherry tomato core germplasms as experimental materials,the genetic diversity of seven agronomic traits were analyzed.The correlation between any two of the seven agronomic traits and the genetic relationships of these germplasms were analyzed based on genotypic values.The genetic diversity indices of the seven agronomic traits were 4.15,4.13,4.16,4.13,4.13,4.13 and 4.01,respectively,showing that the cherry tomato core collection had abundant genetic diversity.The correlation analysis between traits based on genotype effect values showed that leaf length was significantly correlated with leaf width with the correlation coefficient of 0.56.The fruit width was significantly correlated with fruit length with the correlation coefficient of 0.52.The flesh thickness was significantly correlated with fruit length and fruit width with the correlation coefficients of 0.49 and 0.39,respectively.The single fruit weight was significantly correlated with fruit length,fruit width and flesh thickness with the correlation coefficients of 0.44,0.61 and 0.62,respectively.When the genetic distances between core germplasms of cherry tomato were calculated based on the phenotypic values,65 core germplasms of cherry tomato were divided into three groups with the rescaled distance of 10.When the genetic distances between core germplasms of cherry tomato were calculated based on the genotypic values,the 65 core germplasms of cherry tomato were divided into four groups with the rescaled distance of 10.Comparing the 2 clustering results,it could find that genotypic value-based clustering analysis could better clarify the genetic relationship between core germplasms.This study could provide a theoretical basis for the effective utilization of cherry tomato core germplasms.
