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基于GEO数据库联合网络药理学研究川芎-赤芍药对治疗动脉粥样硬化的药理过程及分子机制
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作者 陈启庭 林洪 +4 位作者 陈利捷 卓超林 岳双冰 金宇 张淼 《中国医院用药评价与分析》 2024年第6期655-661,共7页
目的:探讨活血化瘀中药药对川芎-赤芍拮抗动脉粥样硬化的潜在分子机制及药理过程。方法:使用R语言Limma包分析GEO数据库GSE43292数据集,对有表达差异的动脉粥样硬化基因进行筛选和提取。川芎-赤芍药对所含的化学活性组分及靶点通过中药... 目的:探讨活血化瘀中药药对川芎-赤芍拮抗动脉粥样硬化的潜在分子机制及药理过程。方法:使用R语言Limma包分析GEO数据库GSE43292数据集,对有表达差异的动脉粥样硬化基因进行筛选和提取。川芎-赤芍药对所含的化学活性组分及靶点通过中药系统药理学数据库与分析平台检索。合并差异基因和药物作用靶点以获得共同的靶点。利用在线分析工具STRING和Cytoscape构建药物和靶点的调控网络以及靶点间的蛋白质-蛋白质相互作用(PPI)网络。然后利用R语言注释靶点基因的基因本体(GO)功能,分析京都基因与基因组百科全书(KEGG)通路,再进行基因集富集分析(GSEA)以验证KEGG的通路和富集的情况,确定靶点基因的调控功能和参与基因调控功能的信号转导通道。结果:共筛选出动脉粥样硬化差异基因1244个,川芎-赤芍药对含36个生物活性成分,其中靶点为环加氧酶1、肾上腺素受体、过氧化物酶体增殖物激活受体-γ、激酶插入域受体、孕激素受体、基质金属蛋白酶9、CXC趋化因子配体8、蛋白激酶Cβ、白细胞介素6、CD14、二肽基肽酶-4、PIK3CG、肾上腺素受体α1B、微管相关蛋白2、尿激酶纤溶酶原激活剂和单胺氧化酶B。靶点在GO中主要富集在合成DNA过程的调控、DNA生物合成的过程、含胶原的细胞外基质、细胞外基质、蛋白酶结合、细胞迁移、血管形成过程、膜筏结构、转录的调节等与动脉粥样硬化炎症、脂肪代谢及血管生成相关的生物学注释。脂质与动脉粥样硬化通路和核因子κB信号通路与动脉粥样硬化关系最为密切,并且在KEGG信号通路和GSEA均显示出富集。结论:川芎-赤芍药对通过潜在的13个活性成分作用于可能的16个靶点调控相关信号转导通路,通过抗炎、调脂和保护血管等方式产生抗动脉粥样硬化的作用。 展开更多
关键词 川芎-赤芍药对 动脉粥样硬化 geo数据库联合网络药理学 信号通路
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Assessment of Geological Security and Integrated Assessment Geo-environmental Suitability in Worst-hit Areas in Wenchuan Quake 被引量:1
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作者 LI Wenpeng WEN Dongguang +8 位作者 ZHOU Aiguo SUN Xiaoming MENG Hui SHI Jusong CHEN Zongyu ZHANG Liqin FENG Xiaoming LIU Changli ZHOU Jianwei 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2009年第4期816-825,共10页
The Wenchuan earthquake in 2008 and geo-hazards triggered by the earthquake caused large injuries and deaths as well as destructive damage for infrastructures like construction, traffic and electricity. It is urgent t... The Wenchuan earthquake in 2008 and geo-hazards triggered by the earthquake caused large injuries and deaths as well as destructive damage for infrastructures like construction, traffic and electricity. It is urgent to select relatively secure areas for townships and cities constructed in high mountainous regions with high magnitude earthquakes. This paper presents the basic thoughts, evaluation indices and evaluation methods of geological security evaluation, water and land resources security demonstration and integrated assessments of geo-environmental suitability for reconstruction in alp and ravine with high magnitude earthquakes, which are applied in the worst-hit areas (12 counties). The integrated assessment shows that: (1) located in the Longmenshan fault zone, the evaluated area is of poor regional crust stability, in which the unstable and second unstable areas account for 79% of the total; (2) the geo-hazards susceptibility is high in the evaluation area. The spots of geo-hazards triggered by earthquake are mainly distributed along the active fault zone with higher distribution in the moderate and high mountains area, in which the areas of high and moderate susceptibility zoning accounts for 40.1% of the total; (3) geological security is poor in the evaluated area, in which the area of the unsuitable construction occupies 73.1%, whereas in the suitable construction area, the areas of geological security, second security and insecurity zoning account for 8.3 %, 9.3% and 9.3 % of the evaluated area respectively; (4) geo-environmentai suitability is poor in the evaluated area, in which the areas of suitability and basic suitability zoning account for 3.5% and 7.3% of the whole evaluation area. 展开更多
关键词 Wenchuan earthquake geological security carrying capacity of water and land resources geo-environmental suitability
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Geo-environmental Investigations for the Suitability of TSDF Site
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作者 Smita V.Humbarde S.Sankaran 《地学前缘》 EI CAS CSCD 北大核心 2009年第S1期23-23,共1页
A geo-environmental investigation is carried out to identify the suitability for treatment,storage and disposal facility(TSDF) in the industrial area at Perundurai,Tamilnadu(India).State industries promotion corporati... A geo-environmental investigation is carried out to identify the suitability for treatment,storage and disposal facility(TSDF) in the industrial area at Perundurai,Tamilnadu(India).State industries promotion corporation of Tamilnadu(SIPCOT), Perundurai is a fast growing industrial centre therefore,needs a common utility i.e.TSDF site for safe management of the industrial wastes. 展开更多
关键词 TSDF SITE geo-environmental investigation VERTICAL electrical SOUNDINGS Perundhurai INDIA
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Eco-Environmental Geochemistry of Heavy Metal Pollution in Dexing Mining Area 被引量:8
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作者 滕彦国 倪师军 +3 位作者 焦鹏程 邓坚 张成江 王金生 《Chinese Journal Of Geochemistry》 EI CAS 2004年第4期349-358,共10页
An eco-environmental geochemical investigation was carried out in and around the Dexing mining area to determine the concentrations of heavy metals in the surface water, sediments, soils and plants. The main objective... An eco-environmental geochemical investigation was carried out in and around the Dexing mining area to determine the concentrations of heavy metals in the surface water, sediments, soils and plants. The main objective of this study is to assess the environmental situation and evaluate the transferring of heavy metals from mining activities into the food chain. Some samples of water, sediment, topsoil and plant were collected along the Lean River in the Dexing mining area. The total concentrations of Cu, Pb, Zn, Cd, and As were determined by AAS, and Hg was analyzed by cold-vapor AAS. Some indices such as ‘contamination degree’, ‘geoaccumulation index’, and ‘biological absorption coefficient’ were used to assess eco-environmental quality. The investigation indicated a highly localized distribution pattern closely associated with the two pollution sources along the Le’an River bank: one is strong acidity and a large amount of Cu in the drainage from the Dexing Cu mining area; and the other is the high concentrations of Pb and Zn in the effluents released from many smelters and mining, processing and extracting activities in the riparian zone. Results from the investigated localities indicated, at least in part, that some problems associated with environmental quality deterioration should be solved in the future. 展开更多
关键词 经济环境地球化学 污染度 BAC 矿床区域 江西
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Environmental and Geo-Referenced Information Modeling for Sustainable Development in Iran
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作者 Alireza Gharagozlou Mahsa Adl 《Journal of Geographic Information System》 2012年第1期27-32,共6页
By providing Geo-referenced information, which serves as an important tool for decision-making in land use planning, national mapping agencies can help provide effective monitoring of environmental resources while in ... By providing Geo-referenced information, which serves as an important tool for decision-making in land use planning, national mapping agencies can help provide effective monitoring of environmental resources while in many parts of the world lack adequate information in clear. Their role in sustainability and development planning is thus unique and es- sential. National cartographic centers and national mapping agencies offers example of such national mapping agencies. Accurate information on land and environmental resources is essential to effective land use planning. Key sources of in- formation include topographic maps, aerial photos, satellite images, and data derived from geographic information sys- tems (GIS). At the national level, this information can be used to identify environmental resources, reveal the develop- ment potential of the country, and help promote effective ecological studies and sound land use planning with a land use planning approach. This article begins with some general comments on the importance of land use planning, and outli- nes some current environmental issues and also highlights the connection between roles of Geo-referenced information in land use planning with a sustainable development approach. The discussion describes several key methods of resour- ce identification, with particular emphasis on aerial photography and the existing potentials of Iran in this field. The ar- ticle also discusses the role that the Geo-referenced information and environmental planning play in developing and promoting geographic information system use to sustainable development and finally present proper models to use en- vironmental information and geo-referenced data for sustainable development. 展开更多
关键词 GIS RS LAND Use PLANNING geo-Referenced Information SUSTAINABLE Development environment
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网络药理学联合GEO数据挖掘与分子对接探究桔梗-鱼腥草治疗非小细胞肺癌的作用机制 被引量:2
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作者 李永顺 谢嘉嘉 +3 位作者 周继红 张立 陈树超 欧阳子琛 《新中医》 CAS 2023年第15期138-145,共8页
目的:通过网络药理学联合GEO数据挖掘以及分子对接技术探究桔梗-鱼腥草药对治疗非小细胞肺癌(NSCLC)的作用机制。方法:在中药系统药理学数据库与分析平台(TCMSP)数据库搜集桔梗-鱼腥草活性成分及其作用靶点,在Gencards、OMIM和GEO数据... 目的:通过网络药理学联合GEO数据挖掘以及分子对接技术探究桔梗-鱼腥草药对治疗非小细胞肺癌(NSCLC)的作用机制。方法:在中药系统药理学数据库与分析平台(TCMSP)数据库搜集桔梗-鱼腥草活性成分及其作用靶点,在Gencards、OMIM和GEO数据库获取疾病相关靶点,将二者靶点取交集获取桔梗-鱼腥草与NSCLC的共同靶点,并使用Cytoscape3.9.0软件进行药物-活性成分-靶点互作网络构建获取核心成分。通过String网站对药物-疾病共同靶点进行蛋白质-蛋白质相互作用分析得到核心靶点。使用Metascape平台对共同靶点进行GO及KEGG富集分析。最后运用AutoDock和PyMol软件对核心成分和核心靶点进行分子对接和可视化处理。结果:共获得药物活性成分7个,药物-疾病共同靶点139个。其中核心活性成分3个:槲皮素、木犀草素、山奈酚;核心靶点5个:TP53、AKT1、JUN、TNF、EGFR。GO分析得到2281个相关条目。KEGG分析得到326条信号通路。分子对接显示槲皮素、木犀草素、山奈酚与TP53、AKT1、JUN、TNF、EGFR之间均具有较强亲和力。结论:桔梗-鱼腥草药对可能通过TP53、AKT1、JUN、TNF、EGFR等靶点和癌症通路、PI3K-AKT信号通路等途径发挥抗NSCLC的作用。 展开更多
关键词 非小细胞肺癌 桔梗-鱼腥草 网络药理学 geo数据挖掘 分子对接
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慢性间歇低氧和复氧对大鼠胰岛素抵抗及骨骼肌miR-27a-3p/PPARγ/IRS1/PI3K/AKT表达的影响
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作者 周雪利 李华 +5 位作者 陈青宇 靳美娜 李海波 白炜 贾楚璇 魏翠英 《南方医科大学学报》 CAS CSCD 北大核心 2024年第9期1729-1737,共9页
目的探讨慢性间歇低氧(CIH)和复氧对大鼠胰岛素抵抗(IR)及骨骼肌miR-27a-3p/PPARγ/IRS1/PI3K/AKT表达的影响。方法从基因表达数据库(GEO)中下载CIH条件下miRNA数据集,运用DESeq2筛选差异表达最显著的miRNA作为研究对象,使用miRNA walk... 目的探讨慢性间歇低氧(CIH)和复氧对大鼠胰岛素抵抗(IR)及骨骼肌miR-27a-3p/PPARγ/IRS1/PI3K/AKT表达的影响。方法从基因表达数据库(GEO)中下载CIH条件下miRNA数据集,运用DESeq2筛选差异表达最显著的miRNA作为研究对象,使用miRNA walk网站对差异miRNA的靶基因进行基因本体(GO)和京都基因与基因组百科全书(KEGG)富集分析,并Cytoscape软件构建miRNA-mRNA-pathway调控网络。将48只雄性SD大鼠随机分为对照(CON)组和CIH组,24只/组,CON组常氧环境饲养12周,CIH组先予CIH环境饲养8周,再恢复常氧饲养4周。两组均在基线、8周、12周时留取血样和骨骼肌组织,检测空腹血糖(FBG)、空腹胰岛素(FINS)水平,HE染色观察骨骼肌病理改变并计算肌纤维横截面积,实时定量聚合酶链反应(RT-qPCR)和Western blotting法检测骨骼肌miR-27a-3p、过氧化物酶体增殖物激活受体γ(PPARγ)、葡萄糖转运蛋白4(GLUT4)、胰岛素受体1(IRS1)、p-IRS1、磷脂酰肌醇3激酶(PI3K)、磷酸激酶B(AKT)、p-AKT表达,比较组间差异。结果生信分析发现,GEO数据库未筛选出CIH状态下肌肉组织相关的miRNA数据集,仅得到肾脏组织差异表达miRNA的GSE202480数据集,从CON组和CIH组样本中筛选出165个差异表达的miRNA,选最显著miR-27a-3p作为研究对象。GO和KEGG分析显示,差异miRNA的靶基因主要参与肌肉调节和胰岛素信号传导。miRNA-mRNA-pathway调控网络显示miR-27a-3p是PPAR信号通路和PI3K/AKT信号通路的关键调控因子。动物实验发现,基线时,两组各项观察指标均无统计学意义(P>0.05);8周时,与CON组相比,CIH组FBG、FINS、HOMA-IR、PPARγ显著升高(P<0.05或P<0.01),肌纤维排列疏松,肌纤维横截面积、miR-27a-3p、p-IRS1/IRS1、PI3K、p-AKT/AKT显著降低(P<0.05或P<0.01),GLUT4表达呈升高趋势但无统计学意义(P>0.05);12周时,两组各项观察指标均无统计学意义(P>0.05)。结论CIH可导致大鼠IR增加和骨骼肌病理改变,而复氧可以逆转;CIH可致骨骼肌miR-27a-3p表达下调,PPARγ表达上调,IRS1/PI3K/AKT胰岛素信号转导受到抑制,而复氧干预可以逆转;miR-27a-3p可能通过调控PPARγ/IRS1/PI3K/AKT信号通路参与了CIH所致的IR。 展开更多
关键词 慢性间歇低氧-复氧 胰岛素抵抗 geo miR-27a-3p IRS1/PI3K/AKT通路
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Nanoscale mass sensing based on vibration of single-layered graphene sheet in thermal environments 被引量:2
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作者 S.Ahmad Fazelzadeh Esmaeal Ghavanloo 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2014年第1期84-91,共8页
Based on vibration analysis, single-layered graphene sheet (SLGS) with multiple attached nanoparticles is developed as nanoscale mass sensor in thermal environments. Graphene sensors are assumed to be in simplysuppo... Based on vibration analysis, single-layered graphene sheet (SLGS) with multiple attached nanoparticles is developed as nanoscale mass sensor in thermal environments. Graphene sensors are assumed to be in simplysupported configuration. Based on the nonlocal plate the- ory which incorporates size effects into the classical theory, closed-form expressions lot the frequencies and relative fre- quency shills of SLGS-based mass sensor are derived using the Galerkin method. The suggested model is justified by a good agreement between the results given by the present model and available data in literature. The effects of tem- perature difference, nonlocal parameter, the location of the nanoparticle and the number of nanoparticles on the relative frequency shift of the mass sensor are also elucidated. The obtained results show that the sensitivity of the SLGS- based mass sensor increases with increasing temperature difference. 展开更多
关键词 Vibration - Single-layered graphene sheet. Ther- mal environment - Nonlocal elasticity theory Relative frequency shift
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Effect of nitrite intercalated Mg-Al layered double hydroxides on mortar durability under Cl−and SO_(4)^(2-) coexisting environment 被引量:3
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作者 WANG Xu-hui XU Jin-xia TAN Qi-ping 《Journal of Central South University》 SCIE EI CAS CSCD 2022年第2期546-560,共15页
The aim of this paper is to investigate the effect of nitrite intercalated Mg-Al layered double hydroxides(Mg-Al LDH-NO_(2))on mortar durability under the coexisting environment of Cl−and SO_(4)^(2-).Cl−and SO_(4)^(2-... The aim of this paper is to investigate the effect of nitrite intercalated Mg-Al layered double hydroxides(Mg-Al LDH-NO_(2))on mortar durability under the coexisting environment of Cl−and SO_(4)^(2-).Cl−and SO_(4)^(2-) binding properties of Mg-Al LDH-NO_(2) in simulated concrete pore solutions,Cl−and SO_(4)^(2-) diffusion properties of mortars with Mg-Al LDHNO 2 were examined.The steel corrosion and resistance of mortar against SO_(4)^(2-) attack were also evaluated.The results indicate that Mg-Al LDH-NO_(2) can effectively adsorb the Cl−and SO_(4)^(2-) in simulated concrete pore solution,and inhibit the diffusion of Cl−and SO_(4)^(2-) into cement mortars.The presence of SO_(4)^(2-) can greatly affect the uptake amount of Cl−,and there is a coupled effect of Cl−and SO_(4)^(2-) on their penetration into mortar specimens.In addition,Mg-Al LDH-NO_(2) can greatly upgrade the resistance of mortars against SO_(4)^(2-) attack and well prevent the steel from corrosion.However,Cl−will aggravate the SO_(4)^(2-) attack and SO_(4)^(2-) can initially decrease and then increase the steel corrosion. 展开更多
关键词 layered double hydroxides mortar durability SO_(4)^(2-)attack steel corrosion Cl−and SO_(4)^(2-)coexisting environment
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Using GIS Data to Build Informed Virtual Geographic Environments (IVGE) 被引量:2
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作者 Mehdi Mekni 《Journal of Geographic Information System》 2013年第6期548-558,共11页
In this paper, we propose a novel approach to automatically building Informed Virtual Geographic Environments (IVGE) using data provided by Geographic Information Systems (GIS). The obtained IVGE provides 2D and 3D ge... In this paper, we propose a novel approach to automatically building Informed Virtual Geographic Environments (IVGE) using data provided by Geographic Information Systems (GIS). The obtained IVGE provides 2D and 3D geographic information for visualization and simulation purposes. Conventional VGE approaches are generally built upon a grid-based representation, raising the well-known problems of the lack of accuracy of the localized data and the difficulty to merge data with multiple semantics. On the contrary, our approach uses a topological model and provides an exact representation of GIS data, allowing an accurate geometrical exploitation. Moreover, our model can merge semantic information, even if spatially overlapping. In addition, the proposed IVGE contains spatial information which can be enhanced thanks to a geometric abstraction method. We illustrate this model with an application which automatically extracts the required data from standard GIS files and allows a user to navigate and retrieve information from the computed IVGE. 展开更多
关键词 geoGRAPHIC INFORMATION System (GIS) INFORMED VIRTUAL geoGRAPHIC environments (IVGE) MULTI-AGENT geo-Simulation (MAGS)
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Groundwater Conditions and the Geoenvironmental Impacts of the Recent Development in the South Eastern Part of the Western Desert of Egypt
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作者 Ahmed Aziz Abdel Moneim Sameh Zaki Maghawri Diab 《Journal of Water Resource and Protection》 2014年第4期381-401,共21页
The area to the southeast of the Western Desert of Egypt has been subjected to considerable development activities over the last few years. The development includes the cultivation of about 2260 km2 of the desert lan... The area to the southeast of the Western Desert of Egypt has been subjected to considerable development activities over the last few years. The development includes the cultivation of about 2260 km2 of the desert lands “the well-known Toshka Project”. The hydrogeological conditions of the area are subjected to detailed investigation based upon the construction of the water table maps, hydrologeologic cross-sections, pumping tests, aquifer geometry, and recharge-discharge relationship. The study revealed that the Quaternary and the Nubia sediments are the main water bearing layers in the area. The Quaternary aquifer is of limited potential and made of mixed sand with clay deposit ranges in thickness between 5 to 10 m. The Nubia aquifer is the oldest sedimentary formation and the main groundwater resources in the area. It is represented by multilayered of sand and silt exists generally under artesian conditions. It is composed of three water bearing horizons partially separated by two confining horizons and extends in thickness ranges between 70 and 230 meters. The thickness increases away from the high dam lake. The analysis of pumping tests of the aquifer indicated that its potentiality is increasing north of the High Dam Lake (HDL) whereas it decreases in the other direction. This is due to high hydraulic conductivity and aquifer thickness in the area northeast of Khor Toshka and at west of Garf Hussein. The hydraulic conductivity of the aquifer ranges between 12.73 and 0.9 m/day. The review of the changes in groundwater levels in the area showed that there is a drop in ranges between 1 and 14 meters in the last few years indicating that the extraction from the groundwater is much more higher that the replacement rate. Also, the analysis of the fluctuation of water levels of the HDL and the groundwater level indicated that the influence of water on groundwater level in the area is observed only at a distance less than 10 km from the lake shore line. Seepage from the HDL is estimated as 238.13 × 106 m3/year. The geo-environmental impacts of the development on the surface water and groundwater in the area are evaluated. 展开更多
关键词 Toshka Project Groundwater Aswan High Dam LAKE (Nasser Lake) AQUIFER POTENTIALITY geo-environmental Impacts
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Store Environment:Its Importance and Consumers'Expectations on Casual-wear Chain Stores in Honk Kong
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作者 朱惠靖 林美芝 《Journal of China Textile University(English Edition)》 EI CAS 1999年第4期29-33,共5页
This study attempted to examine the importance of storeenvironment in affecting consumers’store choice deci-sion;the relative importance among the three environ-mental factors;and consumers’expectations on store en-... This study attempted to examine the importance of storeenvironment in affecting consumers’store choice deci-sion;the relative importance among the three environ-mental factors;and consumers’expectations on store en-vironment for the casual-wear chain stores in HongKong.Julie Baker’s Three-category framework onstore environments namely ambient,design and socialfactors was adopted for investigation.The results indi-cated that the store environment was important in affect-ing consumers’selection of store for shopping.It alsorevealed that social factor was perceived relatively im-portant than ambient and design factors.Nine store ex-pectation dimensions were categorised and the resultdemonstrated that most respondents were concerned onthe social aspect in their expectation of having a satisfy-ing store environment. 展开更多
关键词 STORE environment casual - WEAR CHAIN STORE FASHION retailing Hong Kong
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Geochemistry of Potentially Toxic Elements in Soil and Sediments of a Tanzanian Small-Scale Gold Mining Area
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作者 Johnbosco Karungamye Mwemezi Rwiza +1 位作者 Juma Selemani Janeth Marwa 《Journal of Geoscience and Environment Protection》 2023年第11期41-61,共21页
Small-scale gold mining is linked to significant environmental pollution by potentially toxic elements (PTEs). However, research on the pollution caused by such mining activities remains insufficient especially in dev... Small-scale gold mining is linked to significant environmental pollution by potentially toxic elements (PTEs). However, research on the pollution caused by such mining activities remains insufficient especially in developing countries. In the present study, a systematic investigation assessed the pollution and level of ecological risk of PTEs in soil and stream sediments in an active small scale gold mining area of Isanga, in Nzega, Tanzania. Samples amounting to 16 soil and 20 sediment were gathered from the study area and analyzed for five PTEs concentrations (As, Cd, Cr, Hg and Pb) using the AAS method. The contamination level and ecological risk were assessed using several pollution indices. The results suggest that the assessed environmental systems of the Isanga mining area and its vicinities are lowly contaminated by PTEs and have a low potential to pose ecological risks. Hg and Cd with mean concentrations of 0.09 mg/kg and 0.26 mg/kg respectively were found to be the most enriched PTEs in soil, compared to their average continental crust concentrations (0.056 mg/kg and 0.102 mg/kg respectively). The levels of the evaluated PTEs in the study area are susceptible to increase over time if proactive steps are not taken to control mining and waste disposal activities. 展开更多
关键词 environmental Pollution Pollution Indices Ecological Risk geo-Accumulation Index Sediment Quality
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China's WTO Accession and Sustainable Development ----- 2002 Report by Task Force on WTO and Environment, November 23-25, 2002, Beijing, China
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《China Population Today》 2002年第Z1期27-36,共10页
关键词 WTO China’s WTO Accession and Sustainable Development November 23-25 Report by Task Force on WTO and environment BEIJING China
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Genetic relationship of rapeseed yield-related traits revealed by elite lines under multiple environments
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作者 Hui WANG Qiong HU +6 位作者 Jun WANG Yunchang LI Li FU Jia LIU Zhongfen WEI Wenxiang WANG Desheng MEI 《Oil Crop Science》 2016年第2期26-34,共9页
To provide a theoretical basis for further improvement of Brassica napus yield, additive dominance with additive - by - additive epistatic effects ( ADAA) genetic model and a 6 X 8 partial dial- lel cross des... To provide a theoretical basis for further improvement of Brassica napus yield, additive dominance with additive - by - additive epistatic effects ( ADAA) genetic model and a 6 X 8 partial dial- lel cross design were used to analyze the genetic effects and correlations of five yield related traits of 14 excellent Brassica napus parental lines and their 46 and F2 populations. The results showed that silique density (SD) , siliques per plant (SPP) , seeds per silique (SPS) and thousand - seed weight (TSW) exhibited not only additive and dominant effects, but also significant epistatic effects. The dominant effects of all five yield - related traits were obviously greater than their additive effects and epistatic effects. Yield per plant (YPP) showed significant genetic correlation with SD, SPP and SPS, and the main component of the genetic correlation was the dominance correlation. SPP and SPS both showed a significant negative correlation with TSW. The SD of rapeseed was genetically correlated with all three components of yield to a certain extent, and there were different components of genetic effects positively correlated with the three yield components, indicating that SD is a potential trait to reconcile the conflict between TSW and SPP as well as SPS. 展开更多
关键词 Brassica napus L. yield related traits correlation analysis genetic effects additive - dominance - epistatic genetic model interaction with environment
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GeO_2-SiO_2纳米复合材料的合成及其光学特性研究 被引量:7
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作者 周延怀 冯玉英 陆海彦 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 2000年第1期23-24,共2页
运用溶胶 凝胶法合成了GeO2 SiO2 纳米复合材料 ,探索了为 pH在 2的条件下通过水解Si(OC2 H5) 4和GeCl4合成GeO2 SiO2 干胶的过程。在 10 0~ 12 0 0℃下对干胶进行热处理。采用UV光谱法测定了材料的光学性质 ,观察到随热处理温度的... 运用溶胶 凝胶法合成了GeO2 SiO2 纳米复合材料 ,探索了为 pH在 2的条件下通过水解Si(OC2 H5) 4和GeCl4合成GeO2 SiO2 干胶的过程。在 10 0~ 12 0 0℃下对干胶进行热处理。采用UV光谱法测定了材料的光学性质 ,观察到随热处理温度的升高 ,样品光谱的吸收边向长波方向移动。由于温度的影响 ,晶粒长大 ,带隙能量减小 ,纳米微晶的量子尺寸效应显示出来。经X射线衍射分析 ,观察到了随热处理温度的增高 ,材料结构从无序到有序的转变过程 ,直到 110 0℃时 ,有明显的微晶生成 ,其尺寸为 5~ 10nm。 展开更多
关键词 溶胶-凝胶 纳米半导体材料 geo2-SiO2
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基于Geo-WebServices的农田环境动态监测与评价分析系统 被引量:5
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作者 王莉 潘瑜春 +2 位作者 王映龙 周艳兵 单东方 《农业工程学报》 EI CAS CSCD 北大核心 2013年第5期109-116,F0002,共9页
为实现农田环境质量数据的在线共享、查询统计与评价分析服务,该文结合地统计学及农田环境质量监测相关专业知识建立采样点布设优选评价模型,并基于GIS、GNSS、角色访问控制等技术,设计开发了农田环境动态监测与评价分析系统。应用证明... 为实现农田环境质量数据的在线共享、查询统计与评价分析服务,该文结合地统计学及农田环境质量监测相关专业知识建立采样点布设优选评价模型,并基于GIS、GNSS、角色访问控制等技术,设计开发了农田环境动态监测与评价分析系统。应用证明,系统采用Geo-WebServices技术能够实现分布式存储的极高分辨率遥感影像和大比例尺基础地理数据等数据服务的实时在线集成与共享,提高了数据分析精度,也解决了高精度基础空间数据的共享安全性及数据版权的问题;能依据角色分配用户权限,使得系统安全可靠;能灵活定制与管理农田环境监测评价与预警分析等模型,并实现从采样布设优选评价、采样点数据预处理、环境质量评价与预警分析及结果多方式直观的可视化等全流程的动态监测分析功能,系统能有效应用于农田环境质量监测与管理。 展开更多
关键词 农田 监测 环境评价系统 geo-WebServices
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生物信息学分析miR-16-5p在乳腺癌中的作用及临床验证
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作者 黄亮 高东 +1 位作者 周丹丹 陈方红 《临床肿瘤学杂志》 CAS 2023年第6期488-494,共7页
目的 生物信息学分析miR-16-5p在乳腺癌(BC)中的作用并进行临床验证。方法 BC的队列数据集来自高通量基因表达数据库(GEO)和癌症基因组图谱(TCGA),并通过生物信息学工具进行分析。在临床验证中,前瞻性纳入2018年1月至2022年2月接受手术... 目的 生物信息学分析miR-16-5p在乳腺癌(BC)中的作用并进行临床验证。方法 BC的队列数据集来自高通量基因表达数据库(GEO)和癌症基因组图谱(TCGA),并通过生物信息学工具进行分析。在临床验证中,前瞻性纳入2018年1月至2022年2月接受手术的BC患者(BC组),获取BC组织和对应的癌旁正常组织。另纳入同期150例体检健康女性作为对照组,获取其血清样本,采用实时荧光定量PCR(qPCR)检测组织及血清miR-16-5p表达。所有BC患者至少随访3年。结果 共鉴定出195个差异表达基因(DEG),根据logFC≥1.5或logFC≤-1.5阈值,miR-16-5p表达显著下调,其受试者工作特征(ROC)曲线和Kaplan-Meier曲线分析显示,miR-16-5p区分BC组织及正常乳腺组织的曲线下面积(AUC)为0.73(95%CI:0.68~0.79),且miR-16-5p低表达的患者预后较差(P=0.013),因此miR-16-5p可作为候选miRNA。在临床验证集中,BC组织miR-16-5p相对表达量明显低于癌旁正常组织[0.46(0.29,0.66)vs. 0.82(0.56,1.08),P<0.001]。且BC患者血清miR-16-5p相对表达量也显著低于对照组[0.34(0.15,0.50)vs. 0.99(0.70,1.20),P<0.001]。ROC曲线显示,当血清miR-16-5p相对表达量≤0.520时可良好地诊断BC患者,AUC值为0.897(95%CI:0.863~0.932)。BC患者血清样本和BC组织样本中miR-16-5p相对表达量呈正相关(rs=0.373,P<0.001)。血清miR-16-5p表达与T分期、N分期、TNM分期有关(P<0.05)。血清miR-16-5p低表达组是影响BC患者患者3年内发生局部复发/远处转移及死亡的独立预测生物标志物(P<0.05)。结论 血清miR-16-5p表达对BC有诊断效能,低血清miR-16-5p表达预示着BC患者更差的疾病进展情况和3年内预后不良。 展开更多
关键词 乳腺癌 geo 癌症基因组图谱 miR-16-5p 临床验证
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基于GEO数据库的黄芪-当归药对治疗糖尿病合并心血管并发症的分子机制研究 被引量:3
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作者 董又滋 赵泉霖 王毓国 《解放军医学杂志》 CAS CSCD 北大核心 2022年第12期1190-1200,共11页
目的探究经典中药配伍组合黄芪-当归药对(芪归药对)对糖尿病合并心血管并发症的治疗作用及分子机制。方法运用BATMAN数据库对芪归药对进行分析,检索GEO数据库中关于糖尿病合并心血管并发症的相关基因芯片数据,借助GEO2R分析差异基因,构... 目的探究经典中药配伍组合黄芪-当归药对(芪归药对)对糖尿病合并心血管并发症的治疗作用及分子机制。方法运用BATMAN数据库对芪归药对进行分析,检索GEO数据库中关于糖尿病合并心血管并发症的相关基因芯片数据,借助GEO2R分析差异基因,构建靶点互作关系网络,拓扑得到芪归药对治疗糖尿病心血管并发症的关键基因;运用DAVID数据库对关键基因进行基因本体功能和KEGG通路富集分析。将20只SD大鼠随机分为健康对照组、糖尿病合并心血管并发症模型组、阳性对照二甲双胍治疗组和芪归药对治疗组,每组5只;除健康对照组外,其余各组均给予高脂高糖饲料并建立糖尿病合并心血管并发症模型;观察各组大鼠心肌细胞中PI3K、Akt3蛋白的表达情况。结果(1)在BATMAN数据库中分析得到芪归药对的相关靶点基因1332个,在GEO数据库中检索得到3个糖尿病合并心血管病变的芯片数据文件(GSE26887、GSE43950、GSE46262),利用GEO2R分析筛选出2759个差异基因;经映射得到芪归药对治疗糖尿病合并心血管并发症的关键基因247个。(2)通过STRING数据库构建关键靶点的差异蛋白相互作用(PPI)网络图,运用Cytoscape 3.7.2构建“芪归药对—活性成分—靶点”网络。采用DAVID数据库进行功能富集分析提示,芪归药对治疗糖尿病合并心血管并发症的生物学进程主要集中在RNA聚合酶Ⅱ启动子转录的正负调控、氧化还原过程、信号转导、凋亡过程、炎症反应、细胞增殖的正调控等,信号通路涉及新陈代谢通路、癌症通路、肿瘤坏死因子(TNF)信号通路、5'-单磷酸(cAMP)信号通路、环磷酸鸟苷酸-蛋白激酶(cGMP-PKG)信号通路、磷脂酰肌醇3-激酶与丝氨酸/苏氨酸激酶(PI3K-Akt)信号通路、非酒精性脂肪肝通路、胰岛素抵抗通路等。(3)采用SD大鼠的动物模型实验结果显示,与健康对照组比较,未接受治疗的糖尿病合并心血管并发症模型组心肌细胞中PI3K、Akt3蛋白表达量均明显降低(P<0.01);芪归药对治疗组和二甲双胍治疗组心肌细胞中PI3K表达量均高于模型组(P<0.01),但低于健康对照组(P<0.01);芪归药对治疗组Akt3蛋白表达量低于健康对照组(P<0.05),二甲双胍治疗组Akt3蛋白表达量高于模型组(P<0.05)。结论芪归药对治疗糖尿病合并心血管并发症的特点是“多成分、多靶点、多途径”,多个关键基因和信号通路发挥了重要作用,进一步研究有望拓宽糖尿病中医药诊疗的思路。 展开更多
关键词 糖尿病合并心血管并发症 黄芪-当归 geo数据库 生物信息学
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基于GEO数据库和网络药理学探讨柴胡-葛根治疗原发性青光眼的机制 被引量:1
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作者 杨稀瑞 王继雪 +2 位作者 袁星星 董霏雪 赵辉 《眼科新进展》 CAS 北大核心 2022年第8期603-607,共5页
目的基于GEO数据库和网络药理学探讨柴胡-葛根治疗原发性青光眼的机制,为原发性青光眼的治疗提供新方向。方法通过TCMSP数据库筛选柴胡、葛根的有效活性成分;通过检索GeneCards、OMIM等数据库及二次挖掘GEO数据库中的芯片数据筛选原发... 目的基于GEO数据库和网络药理学探讨柴胡-葛根治疗原发性青光眼的机制,为原发性青光眼的治疗提供新方向。方法通过TCMSP数据库筛选柴胡、葛根的有效活性成分;通过检索GeneCards、OMIM等数据库及二次挖掘GEO数据库中的芯片数据筛选原发性青光眼的作用靶点;利用David平台对靶基因进行GO功能及KEGG富集分析。体外实验以小鼠视网膜神经节细胞(RGC)为研究对象,以过氧化氢诱导RGC构建氧化应激损伤模型,设置空白组、模型组、空白+中药组、模型+中药组,倒置相差显微镜下观察各组RGC形态变化,RT-PCR检测各组RGC中PI3K和AKT mRNA表达情况。结果网络药理学研究结果证实,与原发性青光眼相关的差异蛋白靶点共63个,包括IL-6、VEGFA等。GO功能及KEGG富集分析结果显示,柴胡-葛根治疗原发性青光眼的生物学过程与蛋白结合、催化、有机环状化合物结合及有机物代谢过程等有关,主要通过调节PI3K-AKT、TNF、MAPK、VEGF、JAK-STAT等信号通路发挥作用。体外实验结果显示,模型+中药组RGC从缓慢贴壁到完全贴壁,并可见少量聚集性生长及串珠样排列,细胞体缓慢增大。RT-PCR检测结果显示,与空白组相比,空白+中药组RGC中PI3K和AKT mRNA表达均下降(均为P<0.05);与模型组相比,模型+中药组RGC中PI3K和AKT mRNA表达均下降(均为P<0.05)。结论柴胡-葛根能够通过抑制PI3K-AKT信号通路抑制细胞凋亡,起到改善原发性青光眼的作用。 展开更多
关键词 geo数据库 网络药理学 柴胡-葛根 原发性青光眼 视网膜神经节细胞
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