Background Global warming leading to heat stress(HS)is becoming a major challenge for broiler production.This study aimed to explore the protective effects of seaweed(Enteromorpha prolifera)polysaccharides(EPS)on the ...Background Global warming leading to heat stress(HS)is becoming a major challenge for broiler production.This study aimed to explore the protective effects of seaweed(Enteromorpha prolifera)polysaccharides(EPS)on the intestinal barrier function,microbial ecology,and performance of broilers under HS.A total of 144 yellow-feathered broilers(male,56 days old)with 682.59±7.38 g were randomly assigned to 3 groups:1)TN(thermal neutral zone,23.6±1.8℃),2)HS(heat stress,33.2±1.5℃ for 10 h/d),and 3)HSE(HS+0.1%EPS).Each group contained 6 replicates with 8 broilers per replicate.The study was conducted for 4 weeks;feed intake and body weights were measured at the end of weeks 2 and 4.At the end of the feeding trial,small intestine samples were collected for histomorphology,antioxidant,secretory immunoglobulin A(s Ig A)content,apoptosis,gene and protein expression analysis;cecal contents were also collected for microbiota analysis based on 16S r DNA sequencing.Results Dietary EPS promoted the average daily gain(ADG)of broilers during 3–4 weeks of HS(P<0.05).At the end of HS on broilers,the activity of total superoxide dismutase(T-SOD),glutathione S-transferase(GST),and the content of s Ig A in jejunum were improved by EPS supplementation(P<0.05).Besides,dietary EPS reduced the epithelial cell apoptosis of jejunum and ileum in heat-stressed broilers(P<0.05).Addition of EPS in HS group broilers'diet upregulated the relative m RNA expression of Occludin,ZO-1,γ-GCLc and IL-10 of the jejunum(P<0.05),whereas downregulated the relative m RNA expression of NF-κB p65,TNF-αand IL-1βof the jejunum(P<0.05).Dietary EPS increased the protein expression of Occludin and ZO-1,whereas it reduced the protein expression of NF-κB p65 and MLCK(P<0.01)and tended to decrease the protein expression of TNF-α(P=0.094)in heat-stressed broilers.Furthermore,the proportions of Bacteroides and Oscillospira among the three groups were positively associated with jejunal apoptosis and pro-inflammatory cytokine expression(P<0.05)and negatively correlated with jejunal Occludin level(P<0.05).However,the proportions of Lactobacillus,Barnesiella,Subdoligranulum,Megasphaera,Collinsella,and Blautia among the three groups were positively related to ADG(P<0.05).Conclusions EPS can be used as a feed additive in yellow-feathered broilers.It effectively improves growth performance and alleviates HS-induced intestinal injury by relieving inflammatory damage and improving the tight junction proteins expression.These beneficial effects may be related to inhibiting NF-κB/MLCK signaling pathway activation and regulation of cecal microbiota.展开更多
[Objective]To investigate the effect of probiotic cocktails on growth performance,intestinal barrier function and antioxidant status in heat-stressed broilers.[Methods]One thousand 1-day-old broilers were randomly div...[Objective]To investigate the effect of probiotic cocktails on growth performance,intestinal barrier function and antioxidant status in heat-stressed broilers.[Methods]One thousand 1-day-old broilers were randomly divided into five groups:the thermoneutral(TN),heat stress(HS),heat stress with antibiotic(HS-AB),heat stress with probiotic cocktail 1(HS-P1),and heat stress with probiotic cocktail 2(HS-P2)groups.The broilers in TN and HS groups received a diet without supplements,and those in HS-AB,HS-P1 and HS-P2 groups were fed a diet supplemented with chlortetracycline(300 g/t)or probiotic cocktail 1 or 2(100 g/t),respectively.[Results]The results showed that heat stress reduced growth performance and increased inflammatory factors,NF-κB and Toll-like receptors in jejunal mucosa coinciding with higher expression of jejunal tight junction proteins,mucin and heat stress protein mRNA.[Conclusion]Dietary probiotic cocktails had protective effects against the heat stress-induced changes in the intestine,with increased serum antioxidant capacity and growth performance of broilers.The effect of these cocktails was similar to that of chlortetracycline.展开更多
Nanostructured zirconia top coat was deposited by air plasma spray and NiCoCrAlTaY bond coat was deposited on Ni substrate by low pressure plasma spray.Nanostructured and conventional thermal barrier coatings were hea...Nanostructured zirconia top coat was deposited by air plasma spray and NiCoCrAlTaY bond coat was deposited on Ni substrate by low pressure plasma spray.Nanostructured and conventional thermal barrier coatings were heat-treated at temperature varying from 1050 to 1 250oC for 2-20 h.The results show that obvious grain growth was found in both nanostructured and conventional thermal barrier coatings(TBCs)after high temperature heat treatment.Monoclinic/tetragonal phases were transformed into cubic phase in the agglomerated nano-powder after calcination.The cubic phase content increased with increasing calcination temperature.Calcination of the powder made the yttria distributed on the surface of the nanocrystalline particles dissolve in zirconia when grains grew.Different from the phase constituent of the as-sprayed conventional TBC which consisted of diffusionlesstransformed tetragonal,the as-sprayed nanostructured TBC consisted of cubic phase.展开更多
Time series measurements (2010–2017) from the Research Moored Array for African–Asian–Australian Monsoon Analysis and Prediction (RAMA) moorings at 15°N,90°E and 12°N,90°E are used to investigat...Time series measurements (2010–2017) from the Research Moored Array for African–Asian–Australian Monsoon Analysis and Prediction (RAMA) moorings at 15°N,90°E and 12°N,90°E are used to investigate the effect of the seasonal barrier layer (BL) on the mixed-layer heat budget in the Bay of Bengal (BoB).The mixed-layer temperature tendency (?T/?t) is primarily controlled by the net surface heat flux that remains in the mixed layer(Q’) from March to October,while both Q’and the vertical heat flux at the base of the mixed layer (Q_(h)),estimated as the residual of the mixed-layer heat budget,dominate during winter (November–February).An inverse relation is observed between the BL thickness and the mixed-layer temperature (MLT).Based on the estimations at the moorings,it is suggested that when the BL thickness is≥25 m,it exerts a considerable influence on ?T/?t through the modulation of Q_(h) (warming) in the BoB.The cooling associated with Q_(h) is strongest when the BL thickness is≤10 m with the MLT exceeding 29°C,while the contribution from Q_(h) remains nearly zero when the BL thickness varies between 10 m and 25 m.Temperature inversion is evident in the BoB during winter when the BL thickness remains≥25 m with an average MLT<28.5°C.Furthermore,Q_(h) follows the seasonal cycle of the BL at these RAMA mooring locations,with r>0.72 at the 95%significance level.展开更多
In the present research,we proposed a scheme to address the issues of severe heat damage,high energy consumption,low cooling system efficiency,and wastage of cold capacity in mines.To elucidate the seasonal variations...In the present research,we proposed a scheme to address the issues of severe heat damage,high energy consumption,low cooling system efficiency,and wastage of cold capacity in mines.To elucidate the seasonal variations of environmental temperature through field measurements,we selected a high-temperature working face in a deep mine as our engineering background.To enhance the heat damage control cability of the working face and minimize unnecessary cooling capac-ity loss,we introduced the multi-dimensional heat hazard prevention and control method called"Heat source barrier and cooling equipment".First,we utilize shotcrete and liquid nitrogen injection to eliminate the heat source and implemented pressure equalization ventilation to disrupt the heat transfer path,thereby creating a heat barrier.Second,we establish divi-sional prediction models for airflow temperature based on the variation patterns obtained through numerical simulation.Third,we devise the location and dynamic control strategy for the cooling equipment based on the prediction models.The results of field application show that the heat resistance and cooling linkage method comply with the safety requirement throughout the entire mining cycle while effectively reducing energy consumption.The ambient temperature is maintained below 30℃,resulting in the energy saving of 10%during the high-temperature period and over 50%during the low-temperature period.These findings serve as a valuable reference for managing heat damage in high-temperature working faces.展开更多
The southeastern Indian Ocean is characterized by the warm barrier layer(BL)underlying the cool mixed layer water in austral winter.This phenomenon lasts almost half a year and thus provides a unique positive effect o...The southeastern Indian Ocean is characterized by the warm barrier layer(BL)underlying the cool mixed layer water in austral winter.This phenomenon lasts almost half a year and thus provides a unique positive effect on the upper mixed layer heat content through the entrainment processes at the base of the mixed layer,which has not been well evaluated due to the lack of proper method and dataset.Among various traditional threshold methods,here it is shown that the 5 m fixed depth difference can produce a reliable and accurate estimate of the entrainment heat flux(EHF)in this BL region.The comparison between the daily and monthly EHF warming indicates that the account for high-frequency EHF variability almost doubles the warming effect in the BL period,which can compensate for or even surpass the surface heat loss.This increased warming is a result of stronger relative rate of the mixed layer deepening and larger temperature differences between the mixed layer and its immediate below in the daily-resolving data.The interannual EHF shows a moderately increasing trend and similar variabilities to the Southern Annular Mode(SAM),likely because the mixed layer deepening under the positive SAM trend is accompanied by enhanced turbulent entrainment and thus increases the BL warming.展开更多
基金funded by the National Nature Science Foundation of China(32002196)。
文摘Background Global warming leading to heat stress(HS)is becoming a major challenge for broiler production.This study aimed to explore the protective effects of seaweed(Enteromorpha prolifera)polysaccharides(EPS)on the intestinal barrier function,microbial ecology,and performance of broilers under HS.A total of 144 yellow-feathered broilers(male,56 days old)with 682.59±7.38 g were randomly assigned to 3 groups:1)TN(thermal neutral zone,23.6±1.8℃),2)HS(heat stress,33.2±1.5℃ for 10 h/d),and 3)HSE(HS+0.1%EPS).Each group contained 6 replicates with 8 broilers per replicate.The study was conducted for 4 weeks;feed intake and body weights were measured at the end of weeks 2 and 4.At the end of the feeding trial,small intestine samples were collected for histomorphology,antioxidant,secretory immunoglobulin A(s Ig A)content,apoptosis,gene and protein expression analysis;cecal contents were also collected for microbiota analysis based on 16S r DNA sequencing.Results Dietary EPS promoted the average daily gain(ADG)of broilers during 3–4 weeks of HS(P<0.05).At the end of HS on broilers,the activity of total superoxide dismutase(T-SOD),glutathione S-transferase(GST),and the content of s Ig A in jejunum were improved by EPS supplementation(P<0.05).Besides,dietary EPS reduced the epithelial cell apoptosis of jejunum and ileum in heat-stressed broilers(P<0.05).Addition of EPS in HS group broilers'diet upregulated the relative m RNA expression of Occludin,ZO-1,γ-GCLc and IL-10 of the jejunum(P<0.05),whereas downregulated the relative m RNA expression of NF-κB p65,TNF-αand IL-1βof the jejunum(P<0.05).Dietary EPS increased the protein expression of Occludin and ZO-1,whereas it reduced the protein expression of NF-κB p65 and MLCK(P<0.01)and tended to decrease the protein expression of TNF-α(P=0.094)in heat-stressed broilers.Furthermore,the proportions of Bacteroides and Oscillospira among the three groups were positively associated with jejunal apoptosis and pro-inflammatory cytokine expression(P<0.05)and negatively correlated with jejunal Occludin level(P<0.05).However,the proportions of Lactobacillus,Barnesiella,Subdoligranulum,Megasphaera,Collinsella,and Blautia among the three groups were positively related to ADG(P<0.05).Conclusions EPS can be used as a feed additive in yellow-feathered broilers.It effectively improves growth performance and alleviates HS-induced intestinal injury by relieving inflammatory damage and improving the tight junction proteins expression.These beneficial effects may be related to inhibiting NF-κB/MLCK signaling pathway activation and regulation of cecal microbiota.
文摘[Objective]To investigate the effect of probiotic cocktails on growth performance,intestinal barrier function and antioxidant status in heat-stressed broilers.[Methods]One thousand 1-day-old broilers were randomly divided into five groups:the thermoneutral(TN),heat stress(HS),heat stress with antibiotic(HS-AB),heat stress with probiotic cocktail 1(HS-P1),and heat stress with probiotic cocktail 2(HS-P2)groups.The broilers in TN and HS groups received a diet without supplements,and those in HS-AB,HS-P1 and HS-P2 groups were fed a diet supplemented with chlortetracycline(300 g/t)or probiotic cocktail 1 or 2(100 g/t),respectively.[Results]The results showed that heat stress reduced growth performance and increased inflammatory factors,NF-κB and Toll-like receptors in jejunal mucosa coinciding with higher expression of jejunal tight junction proteins,mucin and heat stress protein mRNA.[Conclusion]Dietary probiotic cocktails had protective effects against the heat stress-induced changes in the intestine,with increased serum antioxidant capacity and growth performance of broilers.The effect of these cocktails was similar to that of chlortetracycline.
基金Project(1343-77212)supported by the Innovation Program for Graduate Students of Central South University,China
文摘Nanostructured zirconia top coat was deposited by air plasma spray and NiCoCrAlTaY bond coat was deposited on Ni substrate by low pressure plasma spray.Nanostructured and conventional thermal barrier coatings were heat-treated at temperature varying from 1050 to 1 250oC for 2-20 h.The results show that obvious grain growth was found in both nanostructured and conventional thermal barrier coatings(TBCs)after high temperature heat treatment.Monoclinic/tetragonal phases were transformed into cubic phase in the agglomerated nano-powder after calcination.The cubic phase content increased with increasing calcination temperature.Calcination of the powder made the yttria distributed on the surface of the nanocrystalline particles dissolve in zirconia when grains grew.Different from the phase constituent of the as-sprayed conventional TBC which consisted of diffusionlesstransformed tetragonal,the as-sprayed nanostructured TBC consisted of cubic phase.
基金The Strategic Priority Research Program of Chinese Academy of Sciences under contract No.XDA 20060502the National Natural Science Foundation of China under contract Nos 41976016, 42076021 and 41521005+4 种基金the Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory under contract No.GML2019ZD0306the Guangdong Basic and Applied Basic Research Foundation under contract No.2021A1515011534the Grant for Innovation Academy of South China Sea Ecology and Environmental Engineering,Chinese Academy of Sciences under contract No.ISEE2021ZD01the Grant for State Key Laboratory of Tropical OceanographySouth China Sea Institute of Oceanology under contract No.LTOZZ2002。
文摘Time series measurements (2010–2017) from the Research Moored Array for African–Asian–Australian Monsoon Analysis and Prediction (RAMA) moorings at 15°N,90°E and 12°N,90°E are used to investigate the effect of the seasonal barrier layer (BL) on the mixed-layer heat budget in the Bay of Bengal (BoB).The mixed-layer temperature tendency (?T/?t) is primarily controlled by the net surface heat flux that remains in the mixed layer(Q’) from March to October,while both Q’and the vertical heat flux at the base of the mixed layer (Q_(h)),estimated as the residual of the mixed-layer heat budget,dominate during winter (November–February).An inverse relation is observed between the BL thickness and the mixed-layer temperature (MLT).Based on the estimations at the moorings,it is suggested that when the BL thickness is≥25 m,it exerts a considerable influence on ?T/?t through the modulation of Q_(h) (warming) in the BoB.The cooling associated with Q_(h) is strongest when the BL thickness is≤10 m with the MLT exceeding 29°C,while the contribution from Q_(h) remains nearly zero when the BL thickness varies between 10 m and 25 m.Temperature inversion is evident in the BoB during winter when the BL thickness remains≥25 m with an average MLT<28.5°C.Furthermore,Q_(h) follows the seasonal cycle of the BL at these RAMA mooring locations,with r>0.72 at the 95%significance level.
基金supported by the National Natural Science Foundation of China (51874281)the Graduate Innovation Program of China University of Mining and Technology (2022WLKXJ006)the Postgraduate Research&Practice Innovation Program of Jiangsu Province (KYCX22_2612).
文摘In the present research,we proposed a scheme to address the issues of severe heat damage,high energy consumption,low cooling system efficiency,and wastage of cold capacity in mines.To elucidate the seasonal variations of environmental temperature through field measurements,we selected a high-temperature working face in a deep mine as our engineering background.To enhance the heat damage control cability of the working face and minimize unnecessary cooling capac-ity loss,we introduced the multi-dimensional heat hazard prevention and control method called"Heat source barrier and cooling equipment".First,we utilize shotcrete and liquid nitrogen injection to eliminate the heat source and implemented pressure equalization ventilation to disrupt the heat transfer path,thereby creating a heat barrier.Second,we establish divi-sional prediction models for airflow temperature based on the variation patterns obtained through numerical simulation.Third,we devise the location and dynamic control strategy for the cooling equipment based on the prediction models.The results of field application show that the heat resistance and cooling linkage method comply with the safety requirement throughout the entire mining cycle while effectively reducing energy consumption.The ambient temperature is maintained below 30℃,resulting in the energy saving of 10%during the high-temperature period and over 50%during the low-temperature period.These findings serve as a valuable reference for managing heat damage in high-temperature working faces.
基金The National Natural Science Foundation of China under contract No.42276003the Oceanic Interdisciplinary Program of Shanghai Jiao Tong University under contract No.SL2021MS021.
文摘The southeastern Indian Ocean is characterized by the warm barrier layer(BL)underlying the cool mixed layer water in austral winter.This phenomenon lasts almost half a year and thus provides a unique positive effect on the upper mixed layer heat content through the entrainment processes at the base of the mixed layer,which has not been well evaluated due to the lack of proper method and dataset.Among various traditional threshold methods,here it is shown that the 5 m fixed depth difference can produce a reliable and accurate estimate of the entrainment heat flux(EHF)in this BL region.The comparison between the daily and monthly EHF warming indicates that the account for high-frequency EHF variability almost doubles the warming effect in the BL period,which can compensate for or even surpass the surface heat loss.This increased warming is a result of stronger relative rate of the mixed layer deepening and larger temperature differences between the mixed layer and its immediate below in the daily-resolving data.The interannual EHF shows a moderately increasing trend and similar variabilities to the Southern Annular Mode(SAM),likely because the mixed layer deepening under the positive SAM trend is accompanied by enhanced turbulent entrainment and thus increases the BL warming.
