Objective: To investigate the effects of ginaton combined with batroxobin on oxidative stress, hemodynamics and coagulation in patients with sudden hearing loss. Methods: According to random data table method, 100 cas...Objective: To investigate the effects of ginaton combined with batroxobin on oxidative stress, hemodynamics and coagulation in patients with sudden hearing loss. Methods: According to random data table method, 100 cases of sudden hearing loss were randomly divided into the control group (n=50) and observation group (n=50), patients in the control group were given batroxobin treatment, the observation group received ginaton combined with batroxobin theraphy.The levels of oxidative stress, hemodynamics and coagulation were compared between the two groups before and after treatment. Results: The levels of WBV, HCT, PV, APTT, PT, TT, PF, SOD, LPO in the two groups before treatment were not statistically significant. After treatment, the levels of WBV, PV in the control group and observation group were respectively (5.33±0.62) mPa?s, (1.73±0.59) mPa?s and (4.07±0.55) mPa?s, (1.32±0.41) mPa?s, which were significantly lower than those before treatment, and after treatment in the observation group the levels of WBV, PV were significantly lower than the control group;After treatment, the levels of APTT, PT, TT, PF in the control group and observation group were respectively (43.75±6.15) s, (18.23±2.02) s, (15.38±2.51) s, (58.61±12.96) μg/L and (53.68±7.16) s, (24.67±4.35) s, (22.51±4.85) s, (41.47±8.63) μg/L, compared with those in the same group before treatment, the levels of APTT, PT and TT were significantly increased, PF level was significantly decreased, and in the observation group APTT, PT and TT levels were significantly higher and PF level was significantly lower than that in the control group. After treatment, the levels of SOD, LPO in the control group and observation group were respectively (101.78±10.53) nu/L, (5.91±0.95) mmol/L and (110.07±12.62) nu/L, (4.68±1.02) mmol/L compared with those in the same group before treatment, the level of SOD were significantly increased, the level of LPO level was significantly decreased, and in the observation group LPO level was significantly lower and SOD level was significantly higher than that in the control group. Conclusion Compared with the use of batroxobin alone, Ginaton combined with batroxobin can better reduce the stress response, improve hemodynamics and coagulation in patients with sudden hearing loss.展开更多
Objective:The objective of this study was to investigate the effects and possible mechanisms of action of ginseng on cerebral vasospasm and early brain injury(EBI)following hemorrhagic stroke.Materials and Methods:Spr...Objective:The objective of this study was to investigate the effects and possible mechanisms of action of ginseng on cerebral vasospasm and early brain injury(EBI)following hemorrhagic stroke.Materials and Methods:Sprague-Dawley(SD)rats(n=48)were randomly divided into sham operation(sham group),subarachnoid hemorrhage(SAH)model(SAH group),normal saline(NS group),and Ginaton(Extract of Ginkgo Biloba Leaves Drops)intervention(gin group)groups.MCP-1 m RNA and tumor necrosis factor levels were detected using reverse transcription-polymerase chain reaction.The relative expression of m RNA was detected by Western blotting.Results:(1)Compared with the sham group,the SAH,NS,and gin groups had different degrees of neurological dysfunction.Compared with the SAH and NS groups,the neurological deficits in the gin group were significantly improved(P<0.05).(2)Compared with the sham group,the relative expression levels of MCP-1 m RNA in the SAH,NS,and gin groups were 5.1±0.9,3.4±0.6,and 2.5±0.4,respectively;the relative expression levels of m RNA were 13.3±2.4,11.2±1.8,and 3.8±0.6,respectively.(3)The apoptosis rates of brain tissue in the sham,SAH,NS,and gin groups were 4.8±0.7,54.2±10.3,50.1±7.4,and 28.4±4.5,respectively.(4)Western blot showed that the relative expression levels of toll-like receptor-4(TLR-4)protein in the sham,SAH,NS,and gin groups were 0.29±0.03,0.87±0.15,0.65±0.13,and 0.41±0.17,respectively;the relative expression levels of B protein were 0.21±0.04,0.96±0.14,0.73±0.18,and 0.30±0.05,respectively.Gin treatment could inhibit TLR-4 and nuclear factor-κB(NF-κB)protein expression.Conclusions:Dona tablets may inhibit activation of the NF-κB signaling pathway,and SAH-induced inflammatory response,so as to reduce cerebral vasospasm and EBI.展开更多
文摘Objective: To investigate the effects of ginaton combined with batroxobin on oxidative stress, hemodynamics and coagulation in patients with sudden hearing loss. Methods: According to random data table method, 100 cases of sudden hearing loss were randomly divided into the control group (n=50) and observation group (n=50), patients in the control group were given batroxobin treatment, the observation group received ginaton combined with batroxobin theraphy.The levels of oxidative stress, hemodynamics and coagulation were compared between the two groups before and after treatment. Results: The levels of WBV, HCT, PV, APTT, PT, TT, PF, SOD, LPO in the two groups before treatment were not statistically significant. After treatment, the levels of WBV, PV in the control group and observation group were respectively (5.33±0.62) mPa?s, (1.73±0.59) mPa?s and (4.07±0.55) mPa?s, (1.32±0.41) mPa?s, which were significantly lower than those before treatment, and after treatment in the observation group the levels of WBV, PV were significantly lower than the control group;After treatment, the levels of APTT, PT, TT, PF in the control group and observation group were respectively (43.75±6.15) s, (18.23±2.02) s, (15.38±2.51) s, (58.61±12.96) μg/L and (53.68±7.16) s, (24.67±4.35) s, (22.51±4.85) s, (41.47±8.63) μg/L, compared with those in the same group before treatment, the levels of APTT, PT and TT were significantly increased, PF level was significantly decreased, and in the observation group APTT, PT and TT levels were significantly higher and PF level was significantly lower than that in the control group. After treatment, the levels of SOD, LPO in the control group and observation group were respectively (101.78±10.53) nu/L, (5.91±0.95) mmol/L and (110.07±12.62) nu/L, (4.68±1.02) mmol/L compared with those in the same group before treatment, the level of SOD were significantly increased, the level of LPO level was significantly decreased, and in the observation group LPO level was significantly lower and SOD level was significantly higher than that in the control group. Conclusion Compared with the use of batroxobin alone, Ginaton combined with batroxobin can better reduce the stress response, improve hemodynamics and coagulation in patients with sudden hearing loss.
基金financially supported by the Xingtai Key Research Plan Project(2021zc096)。
文摘Objective:The objective of this study was to investigate the effects and possible mechanisms of action of ginseng on cerebral vasospasm and early brain injury(EBI)following hemorrhagic stroke.Materials and Methods:Sprague-Dawley(SD)rats(n=48)were randomly divided into sham operation(sham group),subarachnoid hemorrhage(SAH)model(SAH group),normal saline(NS group),and Ginaton(Extract of Ginkgo Biloba Leaves Drops)intervention(gin group)groups.MCP-1 m RNA and tumor necrosis factor levels were detected using reverse transcription-polymerase chain reaction.The relative expression of m RNA was detected by Western blotting.Results:(1)Compared with the sham group,the SAH,NS,and gin groups had different degrees of neurological dysfunction.Compared with the SAH and NS groups,the neurological deficits in the gin group were significantly improved(P<0.05).(2)Compared with the sham group,the relative expression levels of MCP-1 m RNA in the SAH,NS,and gin groups were 5.1±0.9,3.4±0.6,and 2.5±0.4,respectively;the relative expression levels of m RNA were 13.3±2.4,11.2±1.8,and 3.8±0.6,respectively.(3)The apoptosis rates of brain tissue in the sham,SAH,NS,and gin groups were 4.8±0.7,54.2±10.3,50.1±7.4,and 28.4±4.5,respectively.(4)Western blot showed that the relative expression levels of toll-like receptor-4(TLR-4)protein in the sham,SAH,NS,and gin groups were 0.29±0.03,0.87±0.15,0.65±0.13,and 0.41±0.17,respectively;the relative expression levels of B protein were 0.21±0.04,0.96±0.14,0.73±0.18,and 0.30±0.05,respectively.Gin treatment could inhibit TLR-4 and nuclear factor-κB(NF-κB)protein expression.Conclusions:Dona tablets may inhibit activation of the NF-κB signaling pathway,and SAH-induced inflammatory response,so as to reduce cerebral vasospasm and EBI.
