Ginseng root residue,a by-product obtained during ginseng beverage processing,was shown to contain a high amount of ginsenosides.This study aims to prepare nanoemulsion and liposome from American ginseng root residue ...Ginseng root residue,a by-product obtained during ginseng beverage processing,was shown to contain a high amount of ginsenosides.This study aims to prepare nanoemulsion and liposome from American ginseng root residue extract(AGRE-NE and AGRE-LP)for evaluation of anti-fatigue effects in rats.The AGRE-NE was prepared by mixing an appropriate ratio of ginseng root residue extract,soybean oil,lecithin,Tween 80 and deionized water,while the AGRE-LP prepared by a suitable ratio of ginseng root residue,extract,Tween 80,phosphatidylcholine,cholesterol,PEG400 and deionized water.Based on dynamic light scattering and transmission electron microscopic methods,the mean particle size was 10.4 and 12.3 nm for AGRE-NE respectively,while 53.5 and 61.2 nm for AGRE-LP.Also,both AGRE-NE and AGRE-LP showed a high stability over a 90-day storage period at 4 and 25°C,but the former possessed a lower stability at 80 and 100°C.The anti-fatigue experiment using a swimming rat model with caffeine as positive control demonstrated an extension of exhaustive swimming time and elevation of hepatic glycogen as well as a decline in both blood urea nitrogen and lactic acid levels,following administration with high and low doses of ginseng root residue extract,AGRE-NE and AGRE-LP.By comparison,both AGRE-NE and AGRE-LP showed a pronounced anti-fatigue effect in rats,with a great potential to be developed into a functional food or even a botanic drug.展开更多
基金supported by a grant from the Ministry of Science and Technology,Taiwan(MOST 107-2637-E-234-001)Tzong Jao Hang’s Electron Microscope Laboratory,School of Medicine,Fu Jen Catholic University,Taipei,Taiwan,for technical assistance in recording TEM images.
文摘Ginseng root residue,a by-product obtained during ginseng beverage processing,was shown to contain a high amount of ginsenosides.This study aims to prepare nanoemulsion and liposome from American ginseng root residue extract(AGRE-NE and AGRE-LP)for evaluation of anti-fatigue effects in rats.The AGRE-NE was prepared by mixing an appropriate ratio of ginseng root residue extract,soybean oil,lecithin,Tween 80 and deionized water,while the AGRE-LP prepared by a suitable ratio of ginseng root residue,extract,Tween 80,phosphatidylcholine,cholesterol,PEG400 and deionized water.Based on dynamic light scattering and transmission electron microscopic methods,the mean particle size was 10.4 and 12.3 nm for AGRE-NE respectively,while 53.5 and 61.2 nm for AGRE-LP.Also,both AGRE-NE and AGRE-LP showed a high stability over a 90-day storage period at 4 and 25°C,but the former possessed a lower stability at 80 and 100°C.The anti-fatigue experiment using a swimming rat model with caffeine as positive control demonstrated an extension of exhaustive swimming time and elevation of hepatic glycogen as well as a decline in both blood urea nitrogen and lactic acid levels,following administration with high and low doses of ginseng root residue extract,AGRE-NE and AGRE-LP.By comparison,both AGRE-NE and AGRE-LP showed a pronounced anti-fatigue effect in rats,with a great potential to be developed into a functional food or even a botanic drug.
