Bacillus coagulans has been extensively studied so far,but there has been a lack of research on its usage in allergy.In this study,we designed to assess the effect of different concentrations of B.coagulans on food al...Bacillus coagulans has been extensively studied so far,but there has been a lack of research on its usage in allergy.In this study,we designed to assess the effect of different concentrations of B.coagulans on food allergy in a BALB/c mouse model of ovalbumin(OVA)-induced food allergy and its effect on gut microbes.The assessment of symptoms,specific immunoglobulin E(IgE),T-cell differentiation,and related gene expression levels in sensitized mice by assay indicated that high doses of oral B.coagulans could alleviate allergic symptoms.Treatment with B.coagulans,in the high-dose group,significantly reduced IgE and IgG1 levels and modulated the balance of T helper type 1 cell(Th1)and Th2 and the expression of relevant genes in the spleen.16S rRNA analysis showed that probiotics improved the structure of the microbiota,in particular by boosting the percentage of Clostridia,Bacteroides vulgatus and Enterococcus faecium,and by increasing the abundance of microbial species,thereby modulating the immune system.Therefore,this study can provide insights into the practical application of B.coagulans doses to alleviate OVA allergy.展开更多
目的用卵清蛋白(OVA)致敏小鼠特应性皮炎(atopic dermatitis,AD),建立一种更为简便、稳定的小鼠AD模型。方法采用6周龄BALB/c雌性小鼠,第1日腹腔注射200μg/m L OVA溶液0.5 m L系统致敏,第6日皮下注射100μg/m L OVA溶液0.5 m L再次致...目的用卵清蛋白(OVA)致敏小鼠特应性皮炎(atopic dermatitis,AD),建立一种更为简便、稳定的小鼠AD模型。方法采用6周龄BALB/c雌性小鼠,第1日腹腔注射200μg/m L OVA溶液0.5 m L系统致敏,第6日皮下注射100μg/m L OVA溶液0.5 m L再次致敏。并于第18、39日背部局部贴含50μL,1 000μg/m LOVA的1.5 cm2的无菌纱布,每日更换,分2次局部致敏,各持续1周。第46日,观察小鼠皮肤及其病理学改变,酶联免疫吸附法(ELISA)测定血清中总Ig E水平,统计成模率及动物死亡情况。结果 AD模型小鼠皮肤肉眼可见潮红、脱屑、偶见结痂等皮炎改变;病理可见表皮细胞增厚,过度角化,真皮内淋巴细胞等浸润。模型组小鼠血清总Ig E水平为(6 296.17±1 021.28)ng/L,显著高于空白对照组的(1 280.97±501.24)ng/L和生理盐水组的(1 327.57±629.71)ng/L(均P<0.01)。成模率为94%,死亡率6%。结论该方法可以成功建立小鼠AD模型,造模方法简单,成模率高。展开更多
基金financially supported by the National Key R&D Program of China (2019YFC1605003)the Zhejiang Provincial Natural Science Foundation of China (LGN21C200013)。
文摘Bacillus coagulans has been extensively studied so far,but there has been a lack of research on its usage in allergy.In this study,we designed to assess the effect of different concentrations of B.coagulans on food allergy in a BALB/c mouse model of ovalbumin(OVA)-induced food allergy and its effect on gut microbes.The assessment of symptoms,specific immunoglobulin E(IgE),T-cell differentiation,and related gene expression levels in sensitized mice by assay indicated that high doses of oral B.coagulans could alleviate allergic symptoms.Treatment with B.coagulans,in the high-dose group,significantly reduced IgE and IgG1 levels and modulated the balance of T helper type 1 cell(Th1)and Th2 and the expression of relevant genes in the spleen.16S rRNA analysis showed that probiotics improved the structure of the microbiota,in particular by boosting the percentage of Clostridia,Bacteroides vulgatus and Enterococcus faecium,and by increasing the abundance of microbial species,thereby modulating the immune system.Therefore,this study can provide insights into the practical application of B.coagulans doses to alleviate OVA allergy.
基金Projects(82073604, 81903369) supported by the National Natural Science Foundation of ChinaProject(S202010555075) supported by the National Innovation Training Program for College Students,ChinaProject (CX20210962) supported by the Postgraduate Research and Innovation Project of Hunan Province,China。
文摘目的用卵清蛋白(OVA)致敏小鼠特应性皮炎(atopic dermatitis,AD),建立一种更为简便、稳定的小鼠AD模型。方法采用6周龄BALB/c雌性小鼠,第1日腹腔注射200μg/m L OVA溶液0.5 m L系统致敏,第6日皮下注射100μg/m L OVA溶液0.5 m L再次致敏。并于第18、39日背部局部贴含50μL,1 000μg/m LOVA的1.5 cm2的无菌纱布,每日更换,分2次局部致敏,各持续1周。第46日,观察小鼠皮肤及其病理学改变,酶联免疫吸附法(ELISA)测定血清中总Ig E水平,统计成模率及动物死亡情况。结果 AD模型小鼠皮肤肉眼可见潮红、脱屑、偶见结痂等皮炎改变;病理可见表皮细胞增厚,过度角化,真皮内淋巴细胞等浸润。模型组小鼠血清总Ig E水平为(6 296.17±1 021.28)ng/L,显著高于空白对照组的(1 280.97±501.24)ng/L和生理盐水组的(1 327.57±629.71)ng/L(均P<0.01)。成模率为94%,死亡率6%。结论该方法可以成功建立小鼠AD模型,造模方法简单,成模率高。
