Study on the transient expression of GUS gene at different growing stage of Chlorellaellipsoidea using high velocity microprojectiles, the effects of osmosis, the distance between nicroprojec-tile and target cell, bom...Study on the transient expression of GUS gene at different growing stage of Chlorellaellipsoidea using high velocity microprojectiles, the effects of osmosis, the distance between nicroprojec-tile and target cell, bombardment times, are reported in this paper. The results showed that C. ellip-soidea in exponential phase has higer level of transient expression and that treatment with osmosis can im-prove the GUS transient expression notably. The effect of distance or bombardment times was not ob-served.展开更多
Gle2 is a mutant gene that controls glandless trait in cotton plants and seeds. It is an important gene resource to gossypol-free cottonseed breeding. The objective of this research was to develop SSR markers tightly ...Gle2 is a mutant gene that controls glandless trait in cotton plants and seeds. It is an important gene resource to gossypol-free cottonseed breeding. The objective of this research was to develop SSR markers tightly linked with Gle2 by using the F2 segregating population containing 1599 plants derived from the cross of G. hirsutum genetic standard line TM-1 and G. barbadense glandless mutant line Hai-1. Genetic analysis suggested that the Gle2 was an incomplete dominant gene. Based on the backbone of genetic linkage map from G. hirsutum × G. barbadense BC1 published by our laboratory,Gle2 was lo-cated between CIR362 and NAU2251b,NAU3860b,STV033,with a genetic distance 9.27 and 0.96 cM,respectively. This result is useful for cloning Gle2 gene by map-based cloning method.展开更多
以玉米高抗灰斑病自交系齐319与高感病自交系Ye478构建的RILS(重组自交系)为试材,通过两年田间表型鉴定,选取极端表型家系高抗16个,高感15个,利用SSR分子标记,并结合群体分离分析方法(BSA)筛选玉米抗灰斑病连锁标记并进行基因定位。结...以玉米高抗灰斑病自交系齐319与高感病自交系Ye478构建的RILS(重组自交系)为试材,通过两年田间表型鉴定,选取极端表型家系高抗16个,高感15个,利用SSR分子标记,并结合群体分离分析方法(BSA)筛选玉米抗灰斑病连锁标记并进行基因定位。结果表明,在玉米第1连锁群上检测到1个主效抗病基因位点(QTL),与两侧的分子标记umc2614和bnlg1803遗传图距分别为4.74 c M和3.78 c M,该抗病基因位点可解释40.9%的表型变异率,抗病基因来源于齐319,加性效应达到了-7.817 5。展开更多
文摘Study on the transient expression of GUS gene at different growing stage of Chlorellaellipsoidea using high velocity microprojectiles, the effects of osmosis, the distance between nicroprojec-tile and target cell, bombardment times, are reported in this paper. The results showed that C. ellip-soidea in exponential phase has higer level of transient expression and that treatment with osmosis can im-prove the GUS transient expression notably. The effect of distance or bombardment times was not ob-served.
基金Supported by the National Basic Research Program of China (Grant No. 2007CB108805)Program for Changjiang Scholars and Innovative Re-search Team in University (Grant No. IRT0432)
文摘Gle2 is a mutant gene that controls glandless trait in cotton plants and seeds. It is an important gene resource to gossypol-free cottonseed breeding. The objective of this research was to develop SSR markers tightly linked with Gle2 by using the F2 segregating population containing 1599 plants derived from the cross of G. hirsutum genetic standard line TM-1 and G. barbadense glandless mutant line Hai-1. Genetic analysis suggested that the Gle2 was an incomplete dominant gene. Based on the backbone of genetic linkage map from G. hirsutum × G. barbadense BC1 published by our laboratory,Gle2 was lo-cated between CIR362 and NAU2251b,NAU3860b,STV033,with a genetic distance 9.27 and 0.96 cM,respectively. This result is useful for cloning Gle2 gene by map-based cloning method.
文摘以玉米高抗灰斑病自交系齐319与高感病自交系Ye478构建的RILS(重组自交系)为试材,通过两年田间表型鉴定,选取极端表型家系高抗16个,高感15个,利用SSR分子标记,并结合群体分离分析方法(BSA)筛选玉米抗灰斑病连锁标记并进行基因定位。结果表明,在玉米第1连锁群上检测到1个主效抗病基因位点(QTL),与两侧的分子标记umc2614和bnlg1803遗传图距分别为4.74 c M和3.78 c M,该抗病基因位点可解释40.9%的表型变异率,抗病基因来源于齐319,加性效应达到了-7.817 5。
