Hydrochemical Characteristics and Solute Dynamics of Meltwater Runoff of Urumqi Glacier No.1,Eastern Tianshan,Northwest China

Hydrochemical characteristics and solute dynamics of bulk meltwater draining from Urumqi Glacier No.1 were investigated in years 2006 and 2007. The glacial meltwater was slightly alkaline with the mean pH of 7.64 and 7.61 in 2006 and 2007, respectively. In the meltwater, the dominant anions were the bicarbonate and sulphate, and the dominant cation was calcium. The concentration of major cations were varied as c（Ca2＋） 〉 c（Mg2＋） 〉 c（K＋） 〉 c（Na＋）, while the order for the cations was c（HCO3-） 〉 c（SO42-） 〉 c（NQ-） 〉 c（C1-）. The total dissolved solids （TDS） in meltwater had inverse relationships with the diurnal discharge. The major ion composition of meltwater was mainly controlled by rock weathering as inferred from the Gibbs model. Furthermore, the ion ratios and Piper diagram indicated that the main processes controlling the meltwater chemistry were carbonate weathering, pyrite weathering and feldspar weathering in rocks, and Ca2＋ and HCQ- were the dominant ions during the carbonate weathering process. Solute flux calculation at Glacier No.1 station suggested that chemical denudation rates were 11.46 and 13.90 tonkm-2.yr-1 in 2006 and 2007, respectively.

60-year changes and mechanisms of Urumqi Glacier No.1 in the eastern Tianshan of China,Central Asia

Worldwide examination of glacier change is based on detailed observations from only a small number of glaciers.The ground-based detailed individual glacier monitoring is of strong need and extremely important in both regional and global scales.A long-term integrated multi-level monitoring has been carried out on Urumqi Glacier No.1(UG1)at the headwaters of the Urumqi River in the eastern Tianshan Mountains of Central Asia since 1959 by the Tianshan Glaciological Station,Chinese Acamedey of Sciences(CAS),and the glaciological datasets promise to be the best in China.The boundaries of all glacier zones moved up,resulting in a shrunk accumulation area.The stratigraphy features of the snowpack on the glacier were found to be significantly altered by climate warming.Mass balances of UG1 show accelerated mass loss since 1960,which were attributed to three mechanisms.The glacier has been contracting at an accelerated rate since 1962,resulting in a total reduction of 0.37 km2 or 19.3%from 1962 to 2018.Glacier runoff measured at the UG1 hydrometeorological station demonstrates a significant increase from 1959 to 2018 with a large interannual fluctuation,which is inversely correlated with the glacier's mass balance.This study analyzes on the changes in glacier zones,mass balance,area and length,and streamflow in the nival glacial catchment over the past 60 years.It provides critical insight into the processes and mechanisms of glacier recession in response to climate change.The results are not only representative of those glaciers in the Tianshan mountains,but also for the continental-type throughout the world.The direct observation data form an essential basis for evaluating mountain glacier changes and the impact of glacier shrinkage on water resources in the interior drainage rivers within the vast arid and semi-arid land in northwestern China as well as Central Asia.

The surface velocity feature of Glacier No.1 at the headwater of Urumqi River,Tianshan Mountain

The movement of a glacier can redistribute glacier mass balance and change water and thermal conditions of the glacier.Thus,the glacier can maintain its dynamic balance.Surface velocity of a glacier is a basic feature of glacier movement.With successive monthly observations from 2006 to 2008,we obtained spatial and temporal variations for surface velocity of Glacier No.1 at the headwater of Urumqi River,Tianshan Mountain.Dynamic simulation was used to verify the findings.Results show that altitudinal distribution of glacier velocity was influenced by synthetic effects such as glacier thickness,slope,and bedrock morphology.However,seasonal variation was influenced by changing glacier thickness.

Multi-decadal variations in glacier flow velocity and the influencing factors of Urumqi Glacier No.1 in Tianshan Mountains, Northwest China

Urumqi Glacier No. 1 is a representative glacier in the inland areas of Central Asia and is the only Chinese reference glacier in the World Glacier Monitoring Service. In this study, we explored multi-decadal variations in the flow velocity of the glacier and the influencing factors based on continuous field observations and path coefficient analysis. Results show that the glacier flow velocity decreased from 5.5 m/a in 1980/1981 to 3.3 m/a in 2010/2011. The annual variation in the direction of glacier flow velocity in the western branch and eastern branch was less than 1°–3°, and the change of glacier flow velocity in the western branch was more dramatic than that in the eastern branch. Glacier flow velocity was influenced by glacier morphology（including glacier area, glacier length, and ice thickness）, glacier mass balance and local climate conditions（air temperature and precipitation）, the glacier morphology being the leading factor. The long-term flow velocity data set of Urumqi Glacier No. 1 contributes to a better understanding of glacier dynamics within the context of climatic warming.

Variations of Laohugou Glacier No.12 in the western Qilian Mountains, China, from 1957 to 2015

Glaciers were solid reservoirs and important water resources in western China,but they were retreating significantly in context of global warming.Laohugou Glacier No.12 was the largest valley glacier in Qilian Mountains.In this study,realtime kinematic(RTK)data,topographic map and World View-2 satellite imagery were used to measure changes in terminus,extent and volume of Laohugou Glacier No.12.Results showed that Laohugou Glacier No.12 was shrinking significantly since 1957.From1960 to 2015,the terminus reduction of Laohugou Glacier No.12 was 402.96 m(3.99%)in total,and glacier length decreased to 9.7 km from 10.1 km.Reduction of glacier area and volume were the most obvious.From 1957 to 2015,glacier area and volume decreased by 1.54 km^2(7.03%)and 0.1816 km^3,respectively.Reduction trend of terminus and area was slowing in 1950-1980s,even stable for a period in the mid-1980s,and then accelerated.Ice core analysis result and nearly meteorological station data shown an increasing trend of temperature in 1957-2015,it was a main reason of continuous retreating of Laohugou Glacier No.12.

Effect of topography on the changes of Urumqi Glacier No.1 in the Chinese Tianshan Mountains

Topography plays an important role in determining the glacier changes.However,topography has often been oversimplified in the studies of the glacier changes.No systematic studies have been conducted to evaluate the relationship between the glacier changes and topographic features.The present study provided a detailed insight into the changes in the two branches(east branch and west branch)of Urumqi Glacier No.1 in the Chinese Tianshan Mountains since 1993 and systematically discussed the effect of topography on the glacier parameters.This study analyzed comprehensive recently observed data(from 1992/1993 to 2018/2019),including mass balance,ice thickness,surface elevation,ice velocity,terminus,and area,and then determined the differences in the changes of the two branches and explored the effect of topography on the glacier changes.We also applied a topographic solar radiation model to analyze the influence of topography on the incoming shortwave radiation(SW_(in))across the entire glacier,focusing on the difference in the SW_(in) between the two branches.The glacier mass balance of the east branch was more negative than that of the west branch from 1992/1993 to 2018/2019,and this was mainly attributed to the lower average altitude of the east branch.Compared with the west branch,the decrease rate of the ice velocity was lower in the east branch owing to its relatively increased slope.The narrow shape of the west branch and its southeast aspect in the earlier period resulted in a larger glacier terminus retreat of the west branch.The spatial variability of the SW_(in) across the glacier surface became much larger as altitude increased.The SW_(in) received by the east branch was slightly larger than that received by the west branch,and the northern aspect could receive more SW_(in),leading to glacier melting.In the future,the difference of the glacier changes between the two branches will continue to exist due to their topographic differences.This work is fundamental to understanding how topographic features affect the glacier changes,and provides information for building different types of relationship between the glacier area and ice volume to promote further studies on the basin-scale glacier classification.

Elevation change of the Urumqi Glacier No.1 derived from Sentinel-1A data

Accurate measurements of glacier elevation changes play a crucial role in various glaciological studies related to glacier dynamics and mass balance. In this paper, glacier elevation changes of Urumqi Glacier No.1 between August 2015 and August 2017 were investigated using Sentinel-1 A data and DInSAR technology. Meanwhile, the atmospheric delay error was corrected with the MODIS MOD05L2 products. The weight selection iteration method was applied to calibrate the glacier elevation changes in the mass balance years 2015-2016 and 2016-2017. Finally, the geodetic method was employed to calculate the elevation change values of individual stakes of Urumqi Glacier No.1. Moreover, the elevation change values corrected by the weight selection iteration method were verified. Results showed as follows:(1) the elevation of Urumqi Glacier No.1 glacier affected by atmospheric delay was 1.270 cm from 2015 to 2016. The glacier elevation affected by atmospheric delay from 2016 to 2017 was 1.071 cm.(2) The elevation change value of Urumqi Glacier No.1 was-1.101 m from 2015 to 2016, and the elevation of Urumqi Glacier No.1 decreased by 1.299 m from 2016 to 2017. The overall thickness of Urumqi Glacier No. 1 was thinning.(3) By comparing the elevation change results of individual stakes with corresponding points corrected by the weight selection iteration method, the mean squared errors of difference were 0.343 m and 0.280 m between the two mass balance years, respectively.(4) The accuracy of elevation change in non-glaciated areas was 0.039 m from 2015 to 2016 and 0.034 m from 2016 to 2017. Therefore, it is reliable to use Sentinel-1 A data and the study method proposed in this paper to calculate the elevation change of mountain glaciers with very low horizontal movement.

Influence of meteorological elements on chemical evolution of snow and ice of Urumqi Glacier No.1,eastern Tianshan Mountains

For most mountain glaciers,chemical components in snowfall are subject to the elution process under the influences of meltwater before they are preserved in ice,creating difficulties for interpreting ice core records.To understand the formation process of ice core records and analyze the influences of meteorological factors on the ice core resolution,we measured ion concentrations of snowpacks from 2003 to 2006 in the PGPI(Program for Glacier Processes Investigation)site of Urumqi Glacier No.1.The ion concentration variation in snowpack exhibits apparent seasonality.In summer,the higher snowmelt rates due to air temperature rise intensify dilution and lead to an exponential decrease in ion concentrations as the accumulated positive temperature increases.In winter,the snow ion concentrations are stable and low as a result of reduced temperature and rare precipitation.Many ions from summer precipitation are leached out by meltwater,and only the precipitation that occurs at the end of the wet season can be preserved.Through tracking the evolution of magnesium ion peaks in the snowpack,it is concluded that the ice core resolution is one year on Urumqi Glacier No.1,albeit 70%of the concentration information is lost.

Study on diversity and temporal-spatial characteristics of eukaryotic microorganisms on Glacier No.1 at the Urumqi River Head,Tianshan

Surface snow samples of different altitudes and snow pit samples were collected from Glacier No. 1 at the Urumqi River Head, Tianshan. Denaturing gradient gel electrophoresis （DGGE） was used to examine the diversity and temporal-spatial characteristics of eukaryotic microorganisms with different altitudes and depths. Results show that the eukaryotic microorganisms belong to four kingdoms--Viridiplantae, Fungi, Amoebozoa, and Alveolata. Among them, algae （especially Chlamydomonadales） were the dominant group. The diversity of eukaryotic microorganisms was negatively correlated with altitude and accumulation time, but positively correlated with 8180 values. These results indicate that temperature is the main factor for the temporal-spatial change of eukaryotic microorganisms, and the diversity of eukaryotic microorganisms could be an index for climate and environmental change.

Chemical Characteristics and Environmental Significance of Fresh Snow Deposition on Urumqi Glacier No.1 of Tianshan Mountains, China

Ice and snow chemistry of alpine glaciers is crucial for the research of regional atmospheric environment change. Fresh snow samples were weekly collected from Urumqi Glacier No.1 in the Tianshan Mountains, Xin- jiang, China, and the chemical characteristics and seasonal variations of major ions, mineral dust, δ18O and trace metals were measured. Results show that the concentrations of major ions in the snow are Ca2+ > SO42-> NH4+ > NO3-> Cl-> Na+ > Mg2+ > K+, in which Ca2+ is the dominant cation, and SO42-is the dominant anion. All major ions have close positive correlations with each other except NO3-. δ18O shows positive correlation with air temperature change during the study period. Mineral dust particle and major ionic concentrations in fresh snow have obvious seasonal change, with high concentration in spring but low concentration in summer and autumn, which indicates that the chemical mass input from Asian dust activity to snow is very significant. Temporal changes of trace metals in fresh snow, e.g., Cd, Pb, Zn, Al, Fe, have shown that human-induced pollution of central Asian region also has large contribution to the snow chemistry on alpine glaciers of the Tianshan Mountains.

The transport of chemical components in homogeneous snowpacks on Urumqi Glacier No.1, eastern Tianshan Mountains, Central Asia

Chemical records from alpine ice cores provide an invaluable source of paleoclimatic and environ- mental information. Not only the atmospheric chemical composition but also depositional and post-depositional processes are recorded within snow/tim strata. To interpret the environmental and climatic significance of ice core records, we studied the variability of glacier snowpack chemistry by investigating homogeneous snowpacks from October 2003 to September 2006 on Urumqi Glacier No. 1 in eastern Tianshan Mountains, Central Asia. Principle Component Analysis of ionic species in dry and wet seasons revealed the impact of meltwater in redistributing ions in the snowpacks. The 1st, 2nd and 3rd principle components for dry seasons differ significantly, reflecting complex associations between depositional or/and post-depositional processes. The variability trend of ionic concentrations during the wet seasons was found to fit a Gauss Function with significant parameters. The elution factor revealed that more than half of ions are leached out during the wet seasons. Differences with respect to ion snowpack mo- bility were found. Of the ions studied SO42- was the most mobile and Mg2＋ the least mobile. A threshold relationship between air temperatures and the elution process was investigated over the study period. The results indicate that the strong melt/ablation processes and iconic redistribution occur at a threshold air temperature of 0℃. The study found that surface melt on the snowpacks is the main factor causing the alteration of the snowpack chemistry. Rainfall also has an impact on the chemistry but plays a less significant role than the surface melt.

The mass-balance characteristics and sensitivities to climate variables of Laohugou Glacier No.12,western Qilian Mountains,China

Due to global warming, glaciers on the Tibetan Plateau(TP) are experiencing widespread shrinkage; however, the mechanisms controlling glacier variations across the TP are still rather unclear, especially on the northeastern TP. In this study, a physically based, distributed surface-energy and mass-balance model was used to simulate glacier mass balance forced by meteorological data. The model was applied to Laohugou No. 12 Glacier, western Qilian Mountains, China, during2010~2012. The simulated albedo and mass balance were validated and calibrated by in situ measurements. The simulated annual glacier-wide mass balances were-385 mm water equivalent(w.e.) in 2010/2011 and-232 mm w.e. in 2011/2012,respectively. The mean equilibrium-line altitude(ELA) was 5,015 m a.s.l., during 2010~2012, which ascended by 215 m compared to that in the 1970 s. The mean accumulation area ratio(AAR) was 39% during the two years. Climatic-sensitivity experiments indicated that the change of glacier mass balance resulting from a 1.5 °C increase in air temperature could be offset by a 30% increase in annual precipitation. The glacier mass balance varied linearly with precipitation, at a rate of130 mm w.e. per 10% change in total precipitation.

The movement features analysis of Laohugou Glacier No.12 in Qilian Mountains

The Laohugou Glacier No. 12 is the largest valley glacier in Qilian Mountains, which is located in northem Qinghai-Tibet Plateau. Movement is the basic characteristic of glaciers, and is also an important distinction from other terrestrial natural ice. Glacier changes not only reflect climate change, but also play an important role in humanity society. In the arid regions of western China, glaciers are becoming an important water source. We use the GPS receiver （South-Lingrui $82） as data platform with the aid of RTK measurement technology to observe the surface velocity of Laohugou Glacier No. 12. Surface velocity data shows that the maximum value appears at an altitude of 4,750-4,850 m during the period of 2008-2009. During this period, the west branch surface velocity reached 32.6 m per year at an altitude near 4,830 m, the east branch surface velocity reached 32.4 m per year at the altitude near 4,770 m. Comparing the surface velocity data during 2008-2009 with observation results in 1959, the glacier velocity slowed down about 11%.

A MODEL SIMULATING THE PROCESSES IN RESPONSES OF GLACIER AND RUNOFF TO CLIMATIC CHANGE─A Case Study of Glacier No.1 in the ■rümqi River, China

This paper presents a dynamic glacier model that simulates the processes in response of Glacier No. 1 in headwaters of the Urumqi River to various future climatic scenarios The results indicate that the Glader No. 1 will continue retreating if current climatic conditions prevail, until it reaches an equilibrium state of 1600 m in length after 700 to 800 years. If air temperature raise 1℃, the glacier would become a hanging glacier with a length of 300 m after 700 to 800 years. Due to its retreat, cooling function of the glacier would be weakened, resulting in the air temperature in glaciated area higher than that in ice-free areas. The results also indicate that the current glacier melt runoff is in higher value period in comparison with the runoff in the equilibrium state under the current climatic condition. If the air temperature continues increasing, however, the runoff would still increase to a new peak and then decrease rapidly.

Ice thickness distribution and volume estimation of Burqin Glacier No.18 in the Chinese Altay Mountains

Information on the thickness distribution and volume of glacier ice is highly important for glaciological applications;however,detailed measurements of the ice thickness of many glaciers in the Chinese Altay Mountains remain lacking.Burqin Glacier No.18 is a northeast-orientated cirque glacier located on the southern side of the Altay Mountains.This study used PulseEKKO®PRO 100A enhancement ground-penetrating radar(GPR)to survey the ice thickness and volume of Burqin Glacier No.18 in summer 2018.Together with GPR surveying,spatial distributed profiles of the GPR measurements were concurrently surveyed using the real-time kinematic(RTK)global navigation satellite system(GNSS,Unistrong E650).Besides,we used QuickBird,WorldView-2,and Landsat TM to delineate accurate boundary of the glacier for undertaking estimation of glacier ice volume.GPR measurements revealed that the basal topography of profile B1-B2 was flat,the basal topography of profile C1-C2 presented a V-type form,and the basal topography of profile D1-D2 had a typical U-type topographic feature because the bedrock near the central elevation of the glacier was relatively flat.The longitudinal profile A1-A2 showed a ladder-like distribution.Glacier ice was thin at the terminus and its thickness increased gradually from the elevation of approximately 2620 m a.s.l.along the main axis of the glacier tongue with an average value of 80(±1)m.The average ice thickness of the glacier was determined as 27(±2)m and its total ice volume was estimated at 0.031(±0.002)km3.Interpretation of remote sensing images indicated that during 1989–2016,the glacier area reduced from 1.30 to 1.17 km2(reduction of 0.37%/a)and the glacier terminus retreated at the rate of 8.48 m/a.The mean ice thickness of Burqin Glacier No.18 was less than that of the majority of other observed glaciers in China,especially those in the Qilian Mountains and Central Chinese Tianshan Mountains;this is probably attributable to differences in glacier type and climatic setting.

Comparisons of stable isotopic fractionation in winter and summer at Baishui Glacier No.1,Mt.Yulong,China

Based on the data of δ^18O in surface snow, snow pits, meltwater and the glacier-fed fiver water at Baishui Glacier No. 1, Mt. Yulong, the isotopic fractionation behaviors in the typical monsoonal temperate glacier system in winter and summer were compared. The results indicate that the isotopic fractionation degree in summer is greater than that in winter, suggesting that the snow/ice melting is more intense in summer. Moreover, whenever it is in winter or summer, from surface snow to meltwater, and to glacier-fed fiver water, the gradient of δ^18O with altitude gradually increases. This shows that the degree of isotopic fractionation gradually strengthens when surface snow is being converted into meltwater and finally into glacial fiver water, which suggests that the influence of post-depositional processes on δ^18O gradient in the monsoonal temperate glacier region differs spatially.

High-precision measurements of the inter-annual evolution for Urumqi Glacier No.1 in eastern Tien Shan,China

High-precision measuring of glacier evolution remains a challenge as the available global and regional remote sensing techniques cannot satisfactorily capture the local-scale processes of most small-and medium-sized mountain glaciers.In this study,we use a high-precision local remote sensing technique,long-range terrestrial laser scanning(TLS),to measure the evolution of Urumqi Glacier No.1 at an annual scale.We found that the dense point clouds derived from the TLS survey can be used to reconstruct glacier surface terrain,with certain details,such as depressions,debris-covered areas,and supra-glacial drainages can be distinguished.The glacier experienced pronounced thickness thinning and continuous retreat over the last four mass-balance years(2015−2019).The mean surface slope of Urumqi Glacier No.1 gradually steepened,which may increase the removal of glacier mass.The glacier was deeply incised by two very prominent primary supra-glacial rivers,and those rivers presented a widening trend.Extensive networks of supra-glacial channels had a significant impact on accelerated glacier mass loss.High-precision measuring is of vital importance to understanding the annual evolution of this type of glacier.

Will Glacier No.1 Tianshan exist in the 22^(nd) century?

Mountains have been described as the water towers of the world. Almost all major rivers have their sources in mountains; glaciers are important water resources that contribute meltwater to river discharge. Glaciers participate in the global water cycle and, with their solid water storage, are an important component of the water balance. As solid reservoirs, glaciers continue to receive the mass nourishment of solid precipitation from the atmosphere, and their meltwater feed and regulate fiver discharge. Physical changes in glaciers are an indicator of climate change. Over the past half century, the global temperature has increased by 1-2 ℃, which emphasizes the urgent task of monitoring glaciers and predicting their trend. As an example, we have investigated, researched, and surveyed Glacier No. 1 in the Urumqi River source, Tianshan （abbr. Glacier No. 1 Tianshan or Glacier No.l） for half a century. We have found an increase by degrees of the glacial regression during the last 400 years and discovered a terminal moraine which is forming today. The global temperature is rising continually, while the local glacial temperature is 0.4 times that of the global temperature change. Thus, we forecast that Glacier No. 1 Tianshan will disappear during the late 21 st Century （2074-2100 A.D.）.

Influence of Sub-Cloud Secondary Evaporation Effects on the Stable Isotopes in Precipitation of Urumqi Glacier No.1,Eastern Tianshan

In arid regions,the stable hydrogen and oxygen isotopic composition in raindrops is often modified by sub-cloud secondary evaporation when they descend from cloud base to ground through the unsaturated air.As a result of kinetic fractionation,the slope and intercept of the δ^(2)H-δ^(18)O correlation equation decrease.The variation of deuterium excess from cloud base to the ground is often used to quantitatively evaluate the influence of secondary evaporation effect on isotopes in precipitation.Based on the event-based precipitation samples collected at Urumqi Glacier No.1,eastern Tianshan during four-year observation,the existence and impact of secondary evaporation effects were analyzed by the methods of isotope-evaporation model.Under high air temperature,small raindrop diameter and precipitation amount,and low relative humidity conditions,the remaining rate of raindrops is small and the change of deuterium excess is large relatively,and the slope and intercept of δ^(2)H-δ^(18)O correlation equation are much lower than those of Global Meteoric Water Line,which mean that the influence secondary evaporation on precipitation enhanced.While on the conditions of low air temperature,high relative humidity,heavy rainfall,and large raindrop diameter,the change of deuterium excess is small relatively and the remaining rate of raindrops is large,and the slope and intercept of δ^(2)H-δ^(18)O correlation equation increase,the secondary evaporation is weakened.The isotope-evaporation model described a good linear correlation between changes of deuterium excess and evaporation proportion with the slope of 0.90‰/%,which indicated that an increase of 1%in evaporation may result in a decrease of deuterium excess about 0.90‰.

LncRNA GNAS-AS1通过调节miR-449a/Notch1轴参与胃癌细胞的增殖和迁移

目的探究长链非编码RNA(LncRNA)GNAS反义RNA1(GNAS-AS1)通过调节miR-449a/缺刻基因1(Notch1)轴对胃癌(GC)细胞增殖和迁移的影响。方法收集四川省人民医院2013年9月至2017年9月30例确诊为GC的患者肿瘤组织与癌旁组织标本;将GC细胞AGS随机分为对照组(Control组)、si-NC组、si-GNAS-AS1组、si-GNAS-AS1+inhibitor NC组、si-GNAS-AS1+miR-449a inhibitor组。实时荧光定量PCR检测GNAS-AS1、miR-449a和Notch1 mRNA的表达;MTT实验、平板克隆形成实验检测增殖;wound healing实验检测细胞迁移;Transwell实验检测细胞侵袭。Western Blot检测Notch1、E-cadherin、Vimentin、N-cadherin蛋白表达。双荧光素酶报告基因实验验证miR-449a和GNAS-AS1、Notch1的关系。结果与癌旁组织相比,肿瘤组织中GNAS-AS1、Notch1 mRNA表达升高,miR-449a表达降低(P<0.05)。与Control组、si-NC组相比,si-GNAS-AS1组AGS细胞GNAS-AS1表达、OD_(490)值、克隆形成数、划痕愈合率、细胞侵袭数目、Notch1、Vimentin、N-cadherin蛋白表达表达降低,miR-449a表达、E-cadherin蛋白表达升高(P<0.05)。与si-GNASAS1组、si-GNAS-AS1+inhibitor NC组相比,si-GNAS-AS1+miR-449a inhibitor组OD_(490)值、划痕愈合率、细胞侵袭数目、Notch1、Vimentin、N-cadherin表达升高(P<0.05),miR-449a表达、E-cadherin蛋白表达降低(P<0.05)。GNAS-AS1靶向负调控miR-449a表达,miR-449a靶向负调控Notch1表达。结论沉默GNAS-AS1可能通过上调miR-449a来抑制Notch1蛋白的表达,从而抑制GC细胞增殖、迁移、侵袭过程。