Pharmacology of Active Components of Glechomae Herba in the Treatment of Bladder Cancer

[Objectives]To explore the action mechanism of Glechomae Herba in the treatment of bladder cancer through network pharmacology.[Methods]TCMSP database retrieval and literature text mining were used to find out the main chemical active components and their targets,and the"component-target"network map was constructed by Cytoscape 3.7.0 software.The CTD database was used to select targets related to bladder cancer,and the interaction between the targets of the main active components of Glechomae Herba and the targets related to bladder cancer was taken.A total of 87 targets were obtained,then imported into the STRING database to obtain PPI network,and the topological analysis of the network was conducted to find out the key targets.The key targets were introduced into the DAVID database,and the biological process and KEGG(Kyoto Encyclopedia of Genes and Genomes)pathway of the key targets in the treatment of bladder cancer were obtained.[Results]Active compound components interfere with the occurrence and development of bladder cancer possibly through bladder cancer signaling pathway,proteoglycan in cancer signaling pathway,HIF-1 signaling pathway,tumor necrosis factor signaling pathway,PI3K-Akt signaling pathway,toll-like receptor signaling pathway,node-like receptor signaling pathway,and MAPK signaling pathway,etc.[Conclusions]The pharmacological mechanism of Glechomae Herba in the treatment of bladder cancer may be realized through multiple components,multiple targets and multiple pathways.In conclusion,Glechomae Herba has certain medicinal value.

连钱草总多酚提取及其抗氧化性分析

以连钱草(Glechoma longituba)为原料,乙醇为溶剂,采用浸提法提取连钱草中总多酚含量并对其抗氧化性进行分析,研究乙醇体积分数、温度、时间和料液比对总多酚含量的影响,通过正交试验对浸提条件进行了优化。结果表明,最佳浸提条件为50%乙醇,温度80℃,时间2.0 h,料液比1∶25(g∶mL)。在此条件下浸提,得到的总多酚含量为4.275 mg/g,且连钱草提取物对自由基具有一定的清除能力。

连钱草挥发油的最佳提取工艺研究

[目的]优选采用压力共沸精馏装置提取连钱草挥发油的最佳工艺。[方法]采用正交试验对连钱草挥发油的提取工艺条件进行优选,以挥发油得率为指标,选用L9(34)正交试验表;考察影响挥发油收率的浸泡时间(A)、料液比(B)、提取时间(C)、NaCl浓度(D)4个因素,每个因素取3个水平。[结果]连钱草挥发油的最佳提取工艺为浸泡时间6 h,料液比1∶16(g/ml),提取时间4 h,NaCl浓度为0;在此条件下连钱草精油的得率为0.03%。[结论]该方法可靠、快捷,准确度高,可用于连钱草挥发油的提取。

A Novel Sesquiterpenoid from Glechoma longituba

A novel sesquiterpenoid was isolated from the whole plant of Glechoma longituba. Its structure was elucidated as 1, 8-epoxy-7（11）-germacren-5-one-12, 8-olide 1 on the basis of spectral evidences.

Soil nutrient patchiness affects nutrient use efficiency,though not photosynthesis and growth of parental Glechoma longituba ramets:both patch contrast and direction matter

Aims Most plants are clonal in nature.Clonal ramets can share water,nutrients and photosynthate,especially when they experience patchy resources.Patch contrast(i.e.a difference in resources among patches)and patch direction(i.e.source–sink relations)are among the basic attributes of spatial patchiness.Here,I hypothesize that young established ramets in nutrient-rich patches support old ramets in nutrient-poor patches when ramets are subjected to different patch contrasts and patch directions.Methods In a greenhouse experiment,old and young ramets of Glechoma longituba were grown in four combinations consisting of patch contrast and patch direction.Minus patch direction refers to a patch combination in which parent ramets grow in nutrient-rich patches while connected daughter ramets grow in nutrient-poor ones and plus patch direction is the opposite direction.Imeasured photosynthesis and fluorescence traits,harvested all ramets,took morphological measures,weighed their dry mass and determined their nutrient uptake and use.Important Findings For parental ramets of G.longituba,patch contrast and patch direction and their interactions had no significant effects on net photosynthetic rate,maximal fluorescence yield,photochemical quenching(quenching refers to any process which decreases the fluorescence intensity of a given substance),non-photochemical quenching,nutrient uptake,biomass and stolon weight ratio.Patch direction alone significantly affected root weight ratio.Large patch contrast enhanced N use efficiency(NUE)and P use efficiency(PUE);plus patch direction decreased NUE,but increased PUE;the patch contrast by patch direction interaction affected PUE and K use efficiency(KUE).There were significant interactions between patch direction and patch contrast on PUE and KUE.It is concluded that soil nutrient patchiness may influence nutrient use strategies,but not nutrient uptake,photosynthesis and growth of parent ramets of G.longituba connected to daughter ramets,and that patch contrast and patch direction jointly affect PUE and KUE.