Glucose isomerase(GI)is an enzyme with high potential applications.Characterization of GI producing bacteria with interesting properties from an industrial point of view is essential.Bacillus sp.,Paenarthrobacter sp.,...Glucose isomerase(GI)is an enzyme with high potential applications.Characterization of GI producing bacteria with interesting properties from an industrial point of view is essential.Bacillus sp.,Paenarthrobacter sp.,Chryseobacterium sp.,Hymenobacter sp.,Mycobacterium sp.,and Stenotrophomonas sp.were isolated from soil samples.Optimization of enzyme production yield was investigated in various fermentation conditions using response surface methodology.All isolates exhibited maximum GI activity at 40℃,pH 6–8 after 4 days of incubation.A mixture of peptone/yeast extract or tryptone/peptone enhanced higher enzyme production.The same trend was observed in fermentation medium containing 1%xylose or 2%–2.5%wheat straw.This study advanced the knowledge of these bacterial isolates in promoting wheat straw as feedstock for the bio-based industry.展开更多
High fructose corn syrup has been industrially produced by converting glucose to fructose by glucose isomerases,tetrameric metalloenzymes widely used in industrial biocatalysis.Advances in enzyme engineering and comme...High fructose corn syrup has been industrially produced by converting glucose to fructose by glucose isomerases,tetrameric metalloenzymes widely used in industrial biocatalysis.Advances in enzyme engineering and commercial production of glucose isomerase have paved the way to explore more efficient variants of these enzymes.The 5-hydroxymethylfurfural can be produced from high fructose corn syrup catalytic dehydration,and it can be further converted into various furanic compounds chemically or biologically for various industrial applications as a promising platform chemical.Although the chemical conversion of 5-hydroxymethylfurfural into furanic compounds has been extensively investigated in recent years,bioconversion has shown promise for its mild conditions due to the harsh chemical reaction conditions.This review discusses pro-tein engineering potential for improving glucose isomerase production and recent advancements in bioconversion of 5-hydroxymethylfurfural into value-added furanic derivatives.It suggests bi-ological strategies for the industrial transformation of 5-hydroxymethylfurfural.展开更多
基金supported by the Natural Science and Engineering Research Council of Canada(NSERC)Discovery Grant(RGPIN-2017-05366)to WQ.
文摘Glucose isomerase(GI)is an enzyme with high potential applications.Characterization of GI producing bacteria with interesting properties from an industrial point of view is essential.Bacillus sp.,Paenarthrobacter sp.,Chryseobacterium sp.,Hymenobacter sp.,Mycobacterium sp.,and Stenotrophomonas sp.were isolated from soil samples.Optimization of enzyme production yield was investigated in various fermentation conditions using response surface methodology.All isolates exhibited maximum GI activity at 40℃,pH 6–8 after 4 days of incubation.A mixture of peptone/yeast extract or tryptone/peptone enhanced higher enzyme production.The same trend was observed in fermentation medium containing 1%xylose or 2%–2.5%wheat straw.This study advanced the knowledge of these bacterial isolates in promoting wheat straw as feedstock for the bio-based industry.
基金supported by the Natural Sciences and Engineering Research Council of Canada(Grant number RGPIN-2017-05366)to WQ.
文摘High fructose corn syrup has been industrially produced by converting glucose to fructose by glucose isomerases,tetrameric metalloenzymes widely used in industrial biocatalysis.Advances in enzyme engineering and commercial production of glucose isomerase have paved the way to explore more efficient variants of these enzymes.The 5-hydroxymethylfurfural can be produced from high fructose corn syrup catalytic dehydration,and it can be further converted into various furanic compounds chemically or biologically for various industrial applications as a promising platform chemical.Although the chemical conversion of 5-hydroxymethylfurfural into furanic compounds has been extensively investigated in recent years,bioconversion has shown promise for its mild conditions due to the harsh chemical reaction conditions.This review discusses pro-tein engineering potential for improving glucose isomerase production and recent advancements in bioconversion of 5-hydroxymethylfurfural into value-added furanic derivatives.It suggests bi-ological strategies for the industrial transformation of 5-hydroxymethylfurfural.
