A facile route for the large scale production of graphene oxide(GO) papers and their mechanical enhancement has been presented in this work. The novel paper-like GO made from individual GO sheets in aqueous suspension...A facile route for the large scale production of graphene oxide(GO) papers and their mechanical enhancement has been presented in this work. The novel paper-like GO made from individual GO sheets in aqueous suspension can be achieved in large scale by a simple drop casting method on hydrophobic substrates.Significant enhancement in mechanical stiffness(341%) and fracture strength(234%) of GO paper have been achieved upon modification with a small amount(less than 10 wt%) of glutaraldehyde(GA). The cross-linking reaction takes place between hydroxyl groups on the surface of GO and aldehyde groups of GA, through forming hemiacetal structure, which can result in distinct mechanical enhancement of the GO papers.展开更多
Objective: Prepare cross-linked HA gels with higher mechanical stability, lower degradation velocity and desirable biocompatibility,so as to extend the usage of HA.Method:1.Test molecular weight of HA (Mr_ HA ) by vis...Objective: Prepare cross-linked HA gels with higher mechanical stability, lower degradation velocity and desirable biocompatibility,so as to extend the usage of HA.Method:1.Test molecular weight of HA (Mr_ HA ) by viscosimetry;2.prepare cross-linked HA gels by DVS, GTA, DEC;3.discuss the cross-linking and degradation procedure;4.evaluate the biocompatibility of the best HA gels. Results:The mechanical stability and durability to degradation of HA-DVS gels are superior to those of other gels, and when HA:DVS = 40:1(g/g), at 35℃ and in 0.2 M NaOH solution, the HA-DVS gel shows the best mechanical stability, and its cytotoxicity reaches class I, hemolysis ratio is lower than 5%. Conclusion:Our HA-DVS gel can be used to prepare biologic scaffolds.展开更多
In this study, we developed a fast, simple and novel process to fabricate cross-linked electro-spun gelatin with limited amounts of glutaraldehyde (GA) using trifluoroacetic acid (TFA) as the solvent. Using SEM, the u...In this study, we developed a fast, simple and novel process to fabricate cross-linked electro-spun gelatin with limited amounts of glutaraldehyde (GA) using trifluoroacetic acid (TFA) as the solvent. Using SEM, the uncross-linked gelatin fibers were determined to have diameters between 50-300 nm, while the cross-linked gelatin electro-spun fibers had diameters between 100-500 nm. FT-IR revealed that the un-cross-linked and cross-linked electro-spun gelatin was fabricated successfully by electro-spinning using TFA as a solvent, which has not been reported until now. Stress-strain curves showed that the addition of small amounts of GA increased the strength of the gelatin by two fold and allowed for the creation of a water insoluble gelatin electro-spun membrane.展开更多
In this paper,the adsorbent for the removal of hexavalent Cr(Chromium)from aqueous solutions has been prepared by modifying chitosan composite with EP(Epichlorohydrin)or GA(Glutaraldehyde).The modified cross-linked ch...In this paper,the adsorbent for the removal of hexavalent Cr(Chromium)from aqueous solutions has been prepared by modifying chitosan composite with EP(Epichlorohydrin)or GA(Glutaraldehyde).The modified cross-linked chitosan was characterized by SEM(Scanning Electron Microscopy)and FT-IR(Fourier Transform Infrared Spectroscopy).Batch adsorption experiments were carried out to evaluate the adsorption of Cr(VI)by the cross-linked chitosan under different conditions.Furthermore,the sorption mechanism of Cr(VI)by the cross-linked chitosan was investigated by applying Langmuir and Freundlich isotherm equations to the data obtained.The concentration of Cr in solution was determined by ICP-MS(Inductively Coupled Plasma Mass Spectrometry).The cross-linked chitosan can be an efficient sorbent for Cr(VI).展开更多
为建立罗丹明B(rhodamine B)的免疫分析方法,采用戊二醛法,将半抗原罗丹明123与载体牛血清白蛋白(BSA)及卵清蛋白(OVA)偶联制备免疫抗原R123-B S A和包被抗原R123-OVA。经动物免疫实验证实人工抗原合成,并制备抗罗丹明123的多克隆抗体,...为建立罗丹明B(rhodamine B)的免疫分析方法,采用戊二醛法,将半抗原罗丹明123与载体牛血清白蛋白(BSA)及卵清蛋白(OVA)偶联制备免疫抗原R123-B S A和包被抗原R123-OVA。经动物免疫实验证实人工抗原合成,并制备抗罗丹明123的多克隆抗体,此抗体同时能够检测食品中的罗丹明B,且最低检测限为0.001ng/mL。展开更多
基金financial supports by the National Natural Science Foundation of China (No. 51102164 and 50902092)Science and Technology Commission of Shanghai Municipality (No. 1052nm06800 and 1052nm02000)+1 种基金Shanghai Pujiang Program (No. 11PJD011)the Program for Professor of Special Appointment (Eastern Scholar) at Shanghai Institutions of Higher Learning
文摘A facile route for the large scale production of graphene oxide(GO) papers and their mechanical enhancement has been presented in this work. The novel paper-like GO made from individual GO sheets in aqueous suspension can be achieved in large scale by a simple drop casting method on hydrophobic substrates.Significant enhancement in mechanical stiffness(341%) and fracture strength(234%) of GO paper have been achieved upon modification with a small amount(less than 10 wt%) of glutaraldehyde(GA). The cross-linking reaction takes place between hydroxyl groups on the surface of GO and aldehyde groups of GA, through forming hemiacetal structure, which can result in distinct mechanical enhancement of the GO papers.
基金The Second Hospital of Tianjin Medical University,300211 Tianjin,China
文摘Objective: Prepare cross-linked HA gels with higher mechanical stability, lower degradation velocity and desirable biocompatibility,so as to extend the usage of HA.Method:1.Test molecular weight of HA (Mr_ HA ) by viscosimetry;2.prepare cross-linked HA gels by DVS, GTA, DEC;3.discuss the cross-linking and degradation procedure;4.evaluate the biocompatibility of the best HA gels. Results:The mechanical stability and durability to degradation of HA-DVS gels are superior to those of other gels, and when HA:DVS = 40:1(g/g), at 35℃ and in 0.2 M NaOH solution, the HA-DVS gel shows the best mechanical stability, and its cytotoxicity reaches class I, hemolysis ratio is lower than 5%. Conclusion:Our HA-DVS gel can be used to prepare biologic scaffolds.
文摘In this study, we developed a fast, simple and novel process to fabricate cross-linked electro-spun gelatin with limited amounts of glutaraldehyde (GA) using trifluoroacetic acid (TFA) as the solvent. Using SEM, the uncross-linked gelatin fibers were determined to have diameters between 50-300 nm, while the cross-linked gelatin electro-spun fibers had diameters between 100-500 nm. FT-IR revealed that the un-cross-linked and cross-linked electro-spun gelatin was fabricated successfully by electro-spinning using TFA as a solvent, which has not been reported until now. Stress-strain curves showed that the addition of small amounts of GA increased the strength of the gelatin by two fold and allowed for the creation of a water insoluble gelatin electro-spun membrane.
文摘In this paper,the adsorbent for the removal of hexavalent Cr(Chromium)from aqueous solutions has been prepared by modifying chitosan composite with EP(Epichlorohydrin)or GA(Glutaraldehyde).The modified cross-linked chitosan was characterized by SEM(Scanning Electron Microscopy)and FT-IR(Fourier Transform Infrared Spectroscopy).Batch adsorption experiments were carried out to evaluate the adsorption of Cr(VI)by the cross-linked chitosan under different conditions.Furthermore,the sorption mechanism of Cr(VI)by the cross-linked chitosan was investigated by applying Langmuir and Freundlich isotherm equations to the data obtained.The concentration of Cr in solution was determined by ICP-MS(Inductively Coupled Plasma Mass Spectrometry).The cross-linked chitosan can be an efficient sorbent for Cr(VI).
文摘为建立罗丹明B(rhodamine B)的免疫分析方法,采用戊二醛法,将半抗原罗丹明123与载体牛血清白蛋白(BSA)及卵清蛋白(OVA)偶联制备免疫抗原R123-B S A和包被抗原R123-OVA。经动物免疫实验证实人工抗原合成,并制备抗罗丹明123的多克隆抗体,此抗体同时能够检测食品中的罗丹明B,且最低检测限为0.001ng/mL。
