n-3 polyunsaturated fatty acids(n-3 PUFA)have biologically important functions in human beings,but their contents in natural oils are usually low.This study investigated the concentration of n-3 PUFA-enriched glycerid...n-3 polyunsaturated fatty acids(n-3 PUFA)have biologically important functions in human beings,but their contents in natural oils are usually low.This study investigated the concentration of n-3 PUFA-enriched glycerides by Candida antarctica lipase A(CAL-A)-catalyzed selective methanolysis of algal oil.First,lipases and acyl acceptors were screened.Subsequently,the methanolysis conditions including methanol concentration in aqueous solution,molar ratio of methanol to oil,reaction temperature,lipase loading and reaction time,were optimized.The results indicated that CAL-A could effectively identify n-3 PUFA during methanolysis.Saturated fatty acids and monounsaturated fatty acids were released from algal oil by CAL-A-catalyzed methanolysis,causing the production of n-3 PUFA-enriched glycerides.Under optimal conditions(3:1 of molar ratio of methanol to algal oil,20%methanol aqueous solution,6%CAL-A dosage,25℃,18 h),the n-3 PUFA content increased from the initial 45.96%to 73.96%,with an n-3 PUFA yield of 81.76%.The reusability of CAL-A demonstrated that under optimal conditions the lipase could be used for 8 times.Therefore,this enzymatic process is efficient for synthesis of n-3 PUFA-enriched glycerides.展开更多
The present study was undertaken to investigate the effect of dietary replacement of commonly used vegetable oil(sunflower oil, SFO) with n-3 polyunsaturated fatty acids(PUFA) rich oil sources on broiler chicken perfo...The present study was undertaken to investigate the effect of dietary replacement of commonly used vegetable oil(sunflower oil, SFO) with n-3 polyunsaturated fatty acids(PUFA) rich oil sources on broiler chicken performance, carcass yield, meat fatty acid composition, keeping quality and sensory attributes of meat. In the current experiment, 300 day-old Krishibro broiler chicks were randomly distributed to 5 dietary groups(50 replicates with 6 chicks in each) prepared by replacing SFO(2% and 3% of diet during starter and finisher periods, respectively) with n-3 PUFA rich soybean oil(SO), mustard oil(MO), linseed oil(LO) or fish oil(FO) on weight basis. Variation in oil sources had no influence(P > 0.05) on performance and carcass yield. Supplementation of MO, LO or FO significantly(P < 0.01) increased the n-3 PUFA, lowered the n-6 PUFA deposition and n-6:n-3 ratio in breast and thigh without affecting the organoleptic characters(appearance, flavour, juiciness, tenderness and overall acceptability) of meat.However, thiobarbituric acid reacting substances concentration in meat was increased(P < 0.01) with LO and FO supplementation compared with SFO. It is concluded that, dietary incorporation of MO, LO or FO at 2% and 3% levels during starter and finisher phase can enrich broiler chicken meat with n-3 PUFA without affecting the bird's performance and sensory characters of meat.展开更多
基金This study was financially supported by"National Natural Science Foundation of China(Grant No.:31972035).
文摘n-3 polyunsaturated fatty acids(n-3 PUFA)have biologically important functions in human beings,but their contents in natural oils are usually low.This study investigated the concentration of n-3 PUFA-enriched glycerides by Candida antarctica lipase A(CAL-A)-catalyzed selective methanolysis of algal oil.First,lipases and acyl acceptors were screened.Subsequently,the methanolysis conditions including methanol concentration in aqueous solution,molar ratio of methanol to oil,reaction temperature,lipase loading and reaction time,were optimized.The results indicated that CAL-A could effectively identify n-3 PUFA during methanolysis.Saturated fatty acids and monounsaturated fatty acids were released from algal oil by CAL-A-catalyzed methanolysis,causing the production of n-3 PUFA-enriched glycerides.Under optimal conditions(3:1 of molar ratio of methanol to algal oil,20%methanol aqueous solution,6%CAL-A dosage,25℃,18 h),the n-3 PUFA content increased from the initial 45.96%to 73.96%,with an n-3 PUFA yield of 81.76%.The reusability of CAL-A demonstrated that under optimal conditions the lipase could be used for 8 times.Therefore,this enzymatic process is efficient for synthesis of n-3 PUFA-enriched glycerides.
文摘The present study was undertaken to investigate the effect of dietary replacement of commonly used vegetable oil(sunflower oil, SFO) with n-3 polyunsaturated fatty acids(PUFA) rich oil sources on broiler chicken performance, carcass yield, meat fatty acid composition, keeping quality and sensory attributes of meat. In the current experiment, 300 day-old Krishibro broiler chicks were randomly distributed to 5 dietary groups(50 replicates with 6 chicks in each) prepared by replacing SFO(2% and 3% of diet during starter and finisher periods, respectively) with n-3 PUFA rich soybean oil(SO), mustard oil(MO), linseed oil(LO) or fish oil(FO) on weight basis. Variation in oil sources had no influence(P > 0.05) on performance and carcass yield. Supplementation of MO, LO or FO significantly(P < 0.01) increased the n-3 PUFA, lowered the n-6 PUFA deposition and n-6:n-3 ratio in breast and thigh without affecting the organoleptic characters(appearance, flavour, juiciness, tenderness and overall acceptability) of meat.However, thiobarbituric acid reacting substances concentration in meat was increased(P < 0.01) with LO and FO supplementation compared with SFO. It is concluded that, dietary incorporation of MO, LO or FO at 2% and 3% levels during starter and finisher phase can enrich broiler chicken meat with n-3 PUFA without affecting the bird's performance and sensory characters of meat.
