目的探讨红细胞分布宽度(RDW)、血小板-淋巴细胞比值(PLR)及血小板胶原受体蛋白Ⅵ(GPⅥ)在冠心病中的水平及临床意义。方法选取2017年1—12月在我院治疗的冠心病患者104例,其中不稳定心绞痛37例(UAP组),心肌梗死32例(AMI组)和稳定型心绞...目的探讨红细胞分布宽度(RDW)、血小板-淋巴细胞比值(PLR)及血小板胶原受体蛋白Ⅵ(GPⅥ)在冠心病中的水平及临床意义。方法选取2017年1—12月在我院治疗的冠心病患者104例,其中不稳定心绞痛37例(UAP组),心肌梗死32例(AMI组)和稳定型心绞痛35例(SAP组),同时选取健康自愿者40名作为对照组,检测各组RDW、PLR、GPⅥ等指标水平,同时采用Gensini评分评估各组病情。结果 UAP组和AMI组RDW、PLR、GPⅥ分别为(15.11±1.60)%和(15.03±1.89)%、(140.41±32.24)和(137.55±30.15)、(62.16±14.46) pg/mL和(60.70±15.51) pg/mL,明显高于对照组和SAP组;UAP组和AMI组肌酸激酶同工酶MB(CK-MB)、心肌肌钙蛋白T(cTnT)、心肌肌钙蛋白I(cTnI)分别为(132.44±24.44)IU/L 和(140.58±30.22)IU/L、(0.94±0.10) g/L和(0.98±0.09) g/L、(6.22±1.24) g/L和(6.48±1.31) g/L,明显高于对照组和SAP组;AMI组Gensini评分为(70.24±9.87)分,明显高于UAP组和SAP组;UAP组Gensini评分为(48.44±10.46)分,明显高于SAP组,差异均有统计学意义( P <0.05);RDW、PLR与cTnI呈正相关( r =0.322和0.311, P <0.05);GPⅥ与cTnI、CK-MB、Gensini评分呈正相关( r =0.307、0.322和0.297, P <0.05)。结论冠心病患者RDW、PLR及GPⅥ明显升高,与心肌损伤标记物存在相关性,其中GPⅥ与冠状动脉病变程度有一定关系。展开更多
During mammalian fertilisation, the zona pellucida （ZP） matrix surrounding the oocyte is responsible for the binding of the spermatozoa to the oocyte and induction of the acrosome reaction （AR） in the ZP-bound spe...During mammalian fertilisation, the zona pellucida （ZP） matrix surrounding the oocyte is responsible for the binding of the spermatozoa to the oocyte and induction of the acrosome reaction （AR） in the ZP-bound spermatozoon. The AR is crucial for the penetration of the ZP matrix by spermatozoa. The ZP matrix in mice is composed of three glycoproteins designated ZP1, ZP2 and ZP3, whereas in humans, it is composed of four （ZP1, ZP2, ZP3 and ZP4）. ZP3 acts as the putative primary sperm receptor and is responsible for AR induction in mice, whereas in humans （in addition to ZP3）, ZP1 and ZP4 also induce the AR. The ability of ZP3 to induce the AR resides in its C-terminal fragment. O-linked glycans are critical for the murine ZP3-mediated AR. However, N-linked glycans of human ZP1, ZP3 and ZP4 have important roles in the induction of the AR. Studies with pharmacological inhibitors showed that the ZP3-induced AR involves the activation of the Gi-coupled receptor pathway, whereas ZP1- and ZP4-mediated ARs are independent of this pathway. The ZP3-induced AR involves the activation of T-type voltage-operated calcium channels （VOCCs）, whereas ZP1- and ZP4-induced ARs involve both T- and L-type VOCCs. To conclude, in mice, ZP3 is primarily responsible for the binding of capacitated spermatozoa to the ZP matrix and induction of the AR, whereas in humans （in addition to ZP3）, ZP1 and ZP4 also participate in these stages of fertilisation.展开更多
Myelin-associated glycoprotein（MAG） inhibits the growth of neurites from nerve cells. Extraction and purification of MAG require complex operations; therefore, we attempted to determine whether commercially availabl...Myelin-associated glycoprotein（MAG） inhibits the growth of neurites from nerve cells. Extraction and purification of MAG require complex operations; therefore, we attempted to determine whether commercially available MAG-Fc can replace endogenous MAG for research purposes. Immunofluorescence using specific antibodies against MAG, Nogo receptor（NgR） and paired immunoglobulin-like receptor B（PirB） was used to determine whether MAG-Fc can be endocytosed by neuro-2a cells. In addition, neurite outgrowth of neuro-2a cells treated with different doses of MAG-Fc was evaluated. Enzyme linked immunosorbent assays were used to measure RhoA activity. Western blot assays were conducted to assess Rho-associated protein kinase（ROCK） phosphorylation. Neuro-2a cells expressed NgR and PirB, and MAG-Fc could be endocytosed by binding to NgR and PirB. This activated intracellular signaling pathways to increase RhoA activity and ROCK phosphorylation, ultimately inhibiting neurite outgrowth. These findings not only verify that MAG-Fc can inhibit the growth of neural neurites by activating RhoA signaling pathways, similarly to endogenous MAG, but also clearly demonstrate that commercial MAG-Fc is suitable for experimental studies of neurite outgrowth.展开更多
OBJECTIVE Salvia miltiorrhiza bunge contains various active constituents,some of which have been developed as commercially available medicine.Moreover,some other ingredients in Salvia miltiorrhiza play great roles in ...OBJECTIVE Salvia miltiorrhiza bunge contains various active constituents,some of which have been developed as commercially available medicine.Moreover,some other ingredients in Salvia miltiorrhiza play great roles in anti-platelet activity.The aim of the present study was to investigate the effects and the underlying mechanism of miltirone,a lipophilic compound of Salvia miltiorrhiza Bunge.METHODS The ability of miltirone to modulate platelet function was investigated by a variety of in vitro and in vivo experiments.Platelet aggregation and dense granule secretion induced by various agonists were measured with platelet aggregometer.Clot retraction and spreading were imaged by digital camera and fl uorescence microscope.Ferric chloride-induced carotid injury model and pulmonary thromboembolism model were used to check miltirone effect in vivo.To elucidate the mechanisms of anti-platelet activity of miltirone,flow cytometry and Western blotting were performed.RESULTS Miltirone(2,4,8 μmol·L^(-1)) was shown to suppress platelet aggregation,dense granule and α granule secretion in a dose-dependent manner.Meanwhile,miltirone inhibited the clot retraction and spreading of washed platelets.It reduced the phosphorylation of PLCγ2,PKC,Akt,GSK3β and ERK1/2 in the downstream signal pathway of collagen receptor.It also reduced the phosphorylation of Src and FAK in the integrin αⅡbβ3 mediated "outside-in" signaling,while it did not suppress the phosphorylation of β3.In addition,miltirone prolonged the occlusion time and reduced collagen/epinephrine induced pulmonary thrombi.CONCLUSION Miltirone suppresses platelet "inside-out" and "outside-in" signaling by affecting PLCγ2/PKC/ERK1/2,PI3K/Akt and Src/FAK signaling.Therefore,miltirone might represent a potential anti-platelet candidate for the prevention of thrombotic disorders.展开更多
文摘目的探讨红细胞分布宽度(RDW)、血小板-淋巴细胞比值(PLR)及血小板胶原受体蛋白Ⅵ(GPⅥ)在冠心病中的水平及临床意义。方法选取2017年1—12月在我院治疗的冠心病患者104例,其中不稳定心绞痛37例(UAP组),心肌梗死32例(AMI组)和稳定型心绞痛35例(SAP组),同时选取健康自愿者40名作为对照组,检测各组RDW、PLR、GPⅥ等指标水平,同时采用Gensini评分评估各组病情。结果 UAP组和AMI组RDW、PLR、GPⅥ分别为(15.11±1.60)%和(15.03±1.89)%、(140.41±32.24)和(137.55±30.15)、(62.16±14.46) pg/mL和(60.70±15.51) pg/mL,明显高于对照组和SAP组;UAP组和AMI组肌酸激酶同工酶MB(CK-MB)、心肌肌钙蛋白T(cTnT)、心肌肌钙蛋白I(cTnI)分别为(132.44±24.44)IU/L 和(140.58±30.22)IU/L、(0.94±0.10) g/L和(0.98±0.09) g/L、(6.22±1.24) g/L和(6.48±1.31) g/L,明显高于对照组和SAP组;AMI组Gensini评分为(70.24±9.87)分,明显高于UAP组和SAP组;UAP组Gensini评分为(48.44±10.46)分,明显高于SAP组,差异均有统计学意义( P <0.05);RDW、PLR与cTnI呈正相关( r =0.322和0.311, P <0.05);GPⅥ与cTnI、CK-MB、Gensini评分呈正相关( r =0.307、0.322和0.297, P <0.05)。结论冠心病患者RDW、PLR及GPⅥ明显升高,与心肌损伤标记物存在相关性,其中GPⅥ与冠状动脉病变程度有一定关系。
文摘During mammalian fertilisation, the zona pellucida （ZP） matrix surrounding the oocyte is responsible for the binding of the spermatozoa to the oocyte and induction of the acrosome reaction （AR） in the ZP-bound spermatozoon. The AR is crucial for the penetration of the ZP matrix by spermatozoa. The ZP matrix in mice is composed of three glycoproteins designated ZP1, ZP2 and ZP3, whereas in humans, it is composed of four （ZP1, ZP2, ZP3 and ZP4）. ZP3 acts as the putative primary sperm receptor and is responsible for AR induction in mice, whereas in humans （in addition to ZP3）, ZP1 and ZP4 also induce the AR. The ability of ZP3 to induce the AR resides in its C-terminal fragment. O-linked glycans are critical for the murine ZP3-mediated AR. However, N-linked glycans of human ZP1, ZP3 and ZP4 have important roles in the induction of the AR. Studies with pharmacological inhibitors showed that the ZP3-induced AR involves the activation of the Gi-coupled receptor pathway, whereas ZP1- and ZP4-mediated ARs are independent of this pathway. The ZP3-induced AR involves the activation of T-type voltage-operated calcium channels （VOCCs）, whereas ZP1- and ZP4-induced ARs involve both T- and L-type VOCCs. To conclude, in mice, ZP3 is primarily responsible for the binding of capacitated spermatozoa to the ZP matrix and induction of the AR, whereas in humans （in addition to ZP3）, ZP1 and ZP4 also participate in these stages of fertilisation.
基金supported by the National Natural Science Foundation of China,No.81171178
文摘Myelin-associated glycoprotein（MAG） inhibits the growth of neurites from nerve cells. Extraction and purification of MAG require complex operations; therefore, we attempted to determine whether commercially available MAG-Fc can replace endogenous MAG for research purposes. Immunofluorescence using specific antibodies against MAG, Nogo receptor（NgR） and paired immunoglobulin-like receptor B（PirB） was used to determine whether MAG-Fc can be endocytosed by neuro-2a cells. In addition, neurite outgrowth of neuro-2a cells treated with different doses of MAG-Fc was evaluated. Enzyme linked immunosorbent assays were used to measure RhoA activity. Western blot assays were conducted to assess Rho-associated protein kinase（ROCK） phosphorylation. Neuro-2a cells expressed NgR and PirB, and MAG-Fc could be endocytosed by binding to NgR and PirB. This activated intracellular signaling pathways to increase RhoA activity and ROCK phosphorylation, ultimately inhibiting neurite outgrowth. These findings not only verify that MAG-Fc can inhibit the growth of neural neurites by activating RhoA signaling pathways, similarly to endogenous MAG, but also clearly demonstrate that commercial MAG-Fc is suitable for experimental studies of neurite outgrowth.
基金supported by National Natural Science Fund of China(81273574) Chinese Herb Key Project by Health and Family Planning Commission of Hubei Province
文摘OBJECTIVE Salvia miltiorrhiza bunge contains various active constituents,some of which have been developed as commercially available medicine.Moreover,some other ingredients in Salvia miltiorrhiza play great roles in anti-platelet activity.The aim of the present study was to investigate the effects and the underlying mechanism of miltirone,a lipophilic compound of Salvia miltiorrhiza Bunge.METHODS The ability of miltirone to modulate platelet function was investigated by a variety of in vitro and in vivo experiments.Platelet aggregation and dense granule secretion induced by various agonists were measured with platelet aggregometer.Clot retraction and spreading were imaged by digital camera and fl uorescence microscope.Ferric chloride-induced carotid injury model and pulmonary thromboembolism model were used to check miltirone effect in vivo.To elucidate the mechanisms of anti-platelet activity of miltirone,flow cytometry and Western blotting were performed.RESULTS Miltirone(2,4,8 μmol·L^(-1)) was shown to suppress platelet aggregation,dense granule and α granule secretion in a dose-dependent manner.Meanwhile,miltirone inhibited the clot retraction and spreading of washed platelets.It reduced the phosphorylation of PLCγ2,PKC,Akt,GSK3β and ERK1/2 in the downstream signal pathway of collagen receptor.It also reduced the phosphorylation of Src and FAK in the integrin αⅡbβ3 mediated "outside-in" signaling,while it did not suppress the phosphorylation of β3.In addition,miltirone prolonged the occlusion time and reduced collagen/epinephrine induced pulmonary thrombi.CONCLUSION Miltirone suppresses platelet "inside-out" and "outside-in" signaling by affecting PLCγ2/PKC/ERK1/2,PI3K/Akt and Src/FAK signaling.Therefore,miltirone might represent a potential anti-platelet candidate for the prevention of thrombotic disorders.
