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Elevated fluid and glycosaminoglycan content in the Achilles tendon contribute to higher intratendinous pressures:Implications for Achilles tendinopathy
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作者 Lauren Pringels Gert-Jan Van Valckenborgh +5 位作者 Patrick Segers Amelie Chevalier Hedwig Stepman Evi Wezenbeek Arne Burssens Luc Vanden Bossche 《Journal of Sport and Health Science》 SCIE CAS CSCD 2024年第6期863-871,共9页
Background:Tendinopathy alters the compositional properties of the Achilles tendon by increasing fluid and glycosaminoglycan content.It has been speculated that these changes may affect intratendinous pressure,but the... Background:Tendinopathy alters the compositional properties of the Achilles tendon by increasing fluid and glycosaminoglycan content.It has been speculated that these changes may affect intratendinous pressure,but the extent of this relationship remains unclear.Therefore,we aimed to investigate the impact of elevated fluid and glycosaminoglycan content on Achilles tendon intratendinous pressure and to determine whether hyaluronidase(HYAL) therapy can intervene in this potential relationship.Methods:Twenty paired fresh-frozen cadaveric Achilles tendons were mounted in a tensile-testing machine and loaded up to 5% strain.Intratendinous resting(at 0% strain) and dynamic pressure(at 5% strain) were assessed using the microcapillary infusion technique.First,intratendinous pressure was measured under native conditions before and after infusion of 2 mL physiological saline.Next,80 mg of glycosaminoglycans were administered bilaterally to the paired tendons.The right tendons were additionally treated with 1500 units of HYAL.Finally,both groups were retested,and the glycosaminoglycan content was analyzed.Results:It was found that both elevated fluid and glycosaminoglycan content resulted in higher intratendinous resting and dynamic pressures(p <0.001).HYAL treatment induced a 2.3-fold reduction in glycosaminoglycan content(p=0.002) and restored intratendinous pressures.Conclusion:The results of this study demonstrated that elevated fluid and glycosaminoglycan content in Achilles tendinopathy contribute to increased intratendinous re sting and dynamic pressures,which can be explained by the associated increased volume and reduced permeability of the tendon matrix,respectively.HYAL degrades glycosaminoglycans sufficiently to lower intratendinous pressures and may,therefore,serve as a promising treatment. 展开更多
关键词 Achilles tendon Achilles tendinopathy Intratendinous pressure glycosaminoglycanS Tendon fluid
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Application of Serum Apelin and MMP-9 in Evaluation of Silicosis and Their Correlation with Lung Function
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作者 Gaocai XIONG Yuanfeng YANG +3 位作者 Jiasong LI Tingqian LIU Bingbing HE Zhuoling LIU 《Agricultural Biotechnology》 CAS 2023年第1期39-42,共4页
[Objectives]This study was conducted to explore the combination of serum Apelin and MMP-9 in the assessment of silicosis and their correlation with lung function. [Methods] From January 2020 to January 2021, eight pat... [Objectives]This study was conducted to explore the combination of serum Apelin and MMP-9 in the assessment of silicosis and their correlation with lung function. [Methods] From January 2020 to January 2021, eight patients with silicosis(including 3, 2 and 3 patients with silicosis in stages I, II and III respectively) were selected as the observation group, and eight persons frees of silicosis were selected as the control group during the same period. All patients were detected for serum APJ endogenous ligand(Apelin) of G protein-coupled receptor, matrix metalloproteinase-9(MMP-9) and pulmonary function indexes. The levels of serum Apelin and MMP-9 and lung function in the two groups were compared, and the correlation between serum Apelin and MMP-9 indexes and lung function was analyzed. [Results] The serum Apelin and lung function in the observation group were lower than those in the control group, and the level of MMP-9 in the observation group was higher than that in the control group, and the differences were statistically significant(P<0.05). The MMP-9 index in patients with silicosis in stage III was higher than those in patients with silicosis in stage II and stage I, and the MMP-9 index in patients with silicosis in stage II was higher than that in patients with silicosis in stage I, and the differences were statistically significant(P<0.05). The serum Apelin and lung function indexes of patients with silicosis in stage III were lower than those of patients with silicosis in stage II and stage I, and the serum Apelin and lung function indexes of patients with silicosis in stage II were lower than those of patients with silicosis in stage I, and the differences were statistically significant(P<0.05). The correlation analysis showed a positive correlation between serum Apelin and lung function indexes, and a negative correlation between MMP-9 and lung function indexes. [Conclusions] A lower serum Apelin and a higher MMP-9 value in patients with silicosis indicate severer lung function impairment, and the combined detection of serum Apelin and MMP-9 is conducive to effective evaluation of the disease of silicosis. The serum Apelin index was negatively correlated with silicosis, and the level of MMP-9 was positively correlated with silicosis, which could provide a scientific basis for clinical diagnosis and treatment. 展开更多
关键词 Serum Apelin MMP-9 silicosis Condition assessment Pulmonary function Correlation study
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Evaluation of Glycosaminoglycan in the Lumbar Disc Using Chemical Exchange Saturation Transfer MR at 3.0 Tesla:Reproducibility and Correlation with Disc Degeneration 被引量:8
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作者 Deng Min Yuan Jing +3 位作者 Chen Wei Tian Queenie Chan James F Griffith Wang Yi Xiang 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2016年第1期47-55,共9页
Objective This study aims to explore the clinical applicability and relevance of giycosaminoglycan Chemical Exchange Saturation Transfer (gagCEST) for intervertebral disc. Methods 25 subjects ranging in age from 24 ... Objective This study aims to explore the clinical applicability and relevance of giycosaminoglycan Chemical Exchange Saturation Transfer (gagCEST) for intervertebral disc. Methods 25 subjects ranging in age from 24 yrs to 74 yrs were enrolled, gagCEST was acquired using a single-slice TSE sequence on a 3T. Saturation used a continuous rectangular RF pulse with B1=0.8 I^T and a fixed duration time =1100 ms. Sagittal image was obtained firstly without saturation pulse, and then saturated images were acquired at 52 offsets ranging from +0.i25 to +_7 parts per million (ppm). MR T2 relaxivity map was acquired at the identical location. Six subjects were scanned twice to assess scan-rescan reproducibility. Results GagCEST intraclass correlation coefficient (ICC) of six subjects was 0.759 for nucleus pulposus (NP) and 0.508 for annulus fibrosus (AF). Bland-Altman plots showed NP had a mean difference of 0.10% (95% limits of agreement: -3.02% to 3.22%); while that of AF was 0.34% (95% limits of agreement: -2.28% to 2.95%). For the 25 subjects, gag CEST in NP decreased as disc degeneration increased, with a similar trend to T2 relaxivity. Gag CEST of AF showed a better correlation with disc degeneration than T2 relaxivity. Conclusion GagCEST in NP and AF decreased as disc degeneration increased, while gagCEST in AF showed a better correlation than T2 relaxivity. 展开更多
关键词 glycosaminoglycan Chemical Exchange Saturation Transfer (CEST) REPRODUCIBILITY Discdegeneration
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Glycosaminoglycan remodeling during diabetes and the role of dietary factors in their modulation 被引量:3
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作者 Vemana Gowd Abhignan Gurukar Nandini D Chilkunda 《World Journal of Diabetes》 SCIE CAS 2016年第4期67-73,共7页
Glycosaminoglycans(GAGs) play a significant role in various aspects of cell physiology.These are complex polymeric molecules characterized by disaccharides comprising of uronic acid and amino sugar.Compounded to the h... Glycosaminoglycans(GAGs) play a significant role in various aspects of cell physiology.These are complex polymeric molecules characterized by disaccharides comprising of uronic acid and amino sugar.Compounded to the heterogeneity,these are variously sulfated and epimerized depending on the class of GAG.Among the various classes of GAG,namely,chondroitin/dermatan sulfate,heparin/heparan sulfate,keratan sulfate and hyaluronic acid(HA),only HA is non-sulfated.GAGs are known to undergo remodeling in various tissues during various pathophysiological conditions,diabetes mellitus being one among them.These changes will likely affect their structure thereby impinging on their functionality.Till date,diabetes has been shown to affect GAGs in organs such as kidney,liver,aorta,skin,erythrocytes,etc.to name a few,with deleterious consequences.One of the mainstays in the treatment of diabetes is though dietary means.Various dietary factors are known to play a significant role in regulating glucose homeostasis.Furthermore,in recent years,there has been a keen interest to decipher the role of dietary factors on GAG metabolism.This review focuses on the remodeling of GAGs in various organs during diabetes and their modulation by dietary factors.While effect of diabetes on GAG metabolism has been worked out quite a bit,studies on the role of dietary factors in their modulation has been few and far between.We have tried our best to give the latest reports available on this subject. 展开更多
关键词 glycosaminoglycanS DIABETES PROTEOGLYCANS REMODELING DIETARY factors
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Enhancement of matrix metalloproteinases 2 and 9 accompanied with neurogenesis following collagen glycosaminoglycan matrix implantation after surgical brain injury 被引量:1
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作者 Wei-Cherng Hsu Chun-Hsien Yu +1 位作者 Woon-Man Kung Kuo-Feng Huang 《Neural Regeneration Research》 SCIE CAS CSCD 2018年第6期1007-1012,共6页
Surgical brain injury may result in irreversible neurological deficits. Our previous report showed that partial regeneration of a traumatic brain lesion is achieved by implantation of collagen glycosaminoglycan(CGM)... Surgical brain injury may result in irreversible neurological deficits. Our previous report showed that partial regeneration of a traumatic brain lesion is achieved by implantation of collagen glycosaminoglycan(CGM). Matrix metalloproteinases(MMPs) may play an important role in neurogenesis but there is currently a lack of studies displaying the relationship between the stimulation of MMPs and neurogenesis after collagen glycosaminoglycan implantation following surgical brain trauma. The present study was carried out to further examine the expression of MMP2 and MMP9 after implantation of collagen glycosaminoglycan(CGM) following surgical brain trauma. Using the animal model of surgically induced brain lesion, we implanted CGM into the surgical trauma. Rats were thus divided into three groups:(1) sham operation group: craniotomy only;(2) lesion(L) group: craniotomy + surgical trauma lesion;(3) lesion + CGM(L + CGM) group: CGM implanted following craniotomy and surgical trauma lesion. Cells positive for SOX2(marker of proliferating neural progenitor cells) and matrix metalloproteinases(MMP2 and MMP9) in the lesion boundary zone were assayed and analyzed by immunofluorescence and ELISA commercial kits, respectively. Our results demonstrated that following implantation of CGM after surgical brain trauma, significant increases in MMP2^+/SOX2^+ cells and MMP9^+/SOX2^+ cells were seen within the lesion boundary zone in the L + CGM group. Tissue protein concentrations of MMP2 and MMP9 also increased after CGM scaffold implantation. These findings suggest that implantation of a CGM scaffold alone after surgical brain trauma can enhance the expression of MMP2 and MMP9 accompanied by neurogenesis. 展开更多
关键词 collagen glycosaminoglycan matrix metalloproteinases surgical brain trauma NEUROGENESIS neural regeneration
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The Effects of Tetrandrine (TT) and Polyvinylpyridine-N-Oxide (PVNO) on Gene Expression of Type Ⅰand Type ⅢCollagens during Experimental Silicosis 被引量:8
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作者 LIU BING-CI HE YU-XIAN +1 位作者 MIAO QING WANG HAI-HUA AND YOU BAO-RONG (Institute of Occupational Medicine, 29 Nan Wei Road,Beijing 100050, China) 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 1994年第3期199-204,共6页
In the screening tests of drugs for silicosis in our laboratory, we found that TT, a type of alkaloid isolated from Stephania tetrandra, could inhibit the development of experimental silicosis of rats and the synthesi... In the screening tests of drugs for silicosis in our laboratory, we found that TT, a type of alkaloid isolated from Stephania tetrandra, could inhibit the development of experimental silicosis of rats and the synthesis of collagen in rat lung. Chest X-rays of silicotic patients treaied with TT for 1-3 years showed obvious changes. The silicotic nodules became smallel and shadows became clearer. PVNO was proved to have anti-silicotic effect on animal and clinically. This presentation reports the effect of them on collagen mRNA.Dot blot results showed that 1 (Ⅰ) and 1 (Ⅲ) mRNA levels increased significantly at 60 and 120 days after the rats were exposed to silica dust. The mRNA levels went down at 1 and 3 months after treated by TT and PVNO. In situ hybridization observation revealed that the silver grains of Type Ⅰand Type Ⅲ collagen were scattered within the fibroblasts in cellular nodules and in thickened interstitium of silicosis tissue. The amounts of mRNA silver grains decreased in the lung tissue treated by TT and PVNO. It was suggested that TT and PVNO may inhibil the gene expression of collagen during silicosis 展开更多
关键词 TT on Gene Expression of Type Collagens during Experimental silicosis PVNO The Effects of Tetrandrine and Polyvinylpyridine-N-Oxide and Type
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A Biochemical Study on Combined Treatment of Experimental Silicosis with Tetradrine-PVNO and Tetradrine-QOHP in Rats 被引量:1
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作者 YU LAN ZOU CHANG-QI +2 位作者 LI YU-RUI QU LEI XING KANG-JI AND DU QING-CHENG (Institute of Occupational Medicine, Chinese Academy of Preventive Medicine, Beijing 100050, China) 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 1995年第3期265-268,共4页
A better understanding is needed to explain the mechanism of therapeutic effect of combined use of tetradrine-PVNO and tetradrine-QOHP which play very important roles in treatment of silicosis. Blood prolidase (PLD), ... A better understanding is needed to explain the mechanism of therapeutic effect of combined use of tetradrine-PVNO and tetradrine-QOHP which play very important roles in treatment of silicosis. Blood prolidase (PLD), monoamine oxidase (MAO) and plasminogen (PLG) in silicotic rats after treatment with tetradrine-PVNO or tetradrine-QOHP were measured. The values obtained were compared with the untreated silicotic rats. It was found that the silicotic rats that received tetradrine-PVNO showed significant increase in PLD and decrease in PLG, but no significant change in MAO. The PLD in plasma of silicotic rats that received tetradrine-QOHP were elevated significantly, but PLG and MAO did not change appreciably. These findings suggest that the combined use of tetradrine-PVNO and tetradrine-QOHP can accelerate the degradation of collagen in silicotic rats 展开更多
关键词 PVN A Biochemical Study on Combined Treatment of Experimental silicosis with Tetradrine-PVNO and Tetradrine-QOHP in Rats
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Assessment of Glycosaminoglycan Content of Lumbar Intervertebral Discs in Patients with Radiculopathy
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作者 Sophie Heüveldop Florian Fichter +5 位作者 Anja Müller-Lutz Markus Konieczny Markus Eichner Hans-Jö rg Wittsack Christoph Schleich 《International Journal of Clinical Medicine》 2019年第4期259-269,共11页
Objective: To assess glycosaminoglycan (GAG) content of lumbar intervertebral discs (IVDs) in patients with radiculopathy compared with healthy volunteers with glycosaminoglycan chemical exchange saturation transfer (... Objective: To assess glycosaminoglycan (GAG) content of lumbar intervertebral discs (IVDs) in patients with radiculopathy compared with healthy volunteers with glycosaminoglycan chemical exchange saturation transfer (gagCEST). Methods: The lumbar spines of 15 patients with radiculopathy (9 women, 6 men;mean age 45 years;range: 19 - 80 years) and 13 healthy controls (10 women, 3 men;mean age 29 years;range: 19 - 38 years) without lumbar back pain or previous spine surgery were examined at a 3 Tesla (T) magnetic resonance imaging (MRI) scanner in this prospective study. The MRI protocol included standard morphological, sagittal, and transverse T2-weighted (T2w) images of the five lumbar IVDs (L1-S1) to assess Pfirrmann score and to detect disc disorders according to the Combined Task Force classification. To analyze biochemically the lumbar IVDs, a gagCEST sequence was applied to measure the GAG content of the nucleus pulposus (NP) and annulus fibrosus (AF). Results: Patients with radiculopathy indicated significantly lower gagCEST values in NP than healthy volunteers (2.82% &plusmn;3.12% vs. 4.09% &plusmn;2.25%, P = 0.017). The GAG content of AF showed no significant difference between volunteers and patients (2.66% &plusmn;2.01% vs. 1.92% &plusmn;2.56%;P = 0.175). Conclusions. Patients with radiculopathy presented with lower GAG values than healthy volunteers in NP, indicating an association between pain and IVD degeneration. gagCEST of lumbar IVDs is a powerful, non-invasive tool to investigate early disc degeneration, which we could demonstrate in the NP in our study collective. 展开更多
关键词 glycosaminoglycan Chemical Exchange Saturation Transfer RADICULOPATHY INTERVERTEBRAL Disc LUMBAR SPINE Early DEGENERATION
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Studies on Structural Changes of Collagen in Silicosis
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作者 LIYU-RUI HUXUN 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 1994年第4期302-306,共5页
In order to provide scientific information on the prevention and treatment of silicosis,studies about changes of silicotic collagen in lungs were carried out. In this paper, we present experiments about the structural... In order to provide scientific information on the prevention and treatment of silicosis,studies about changes of silicotic collagen in lungs were carried out. In this paper, we present experiments about the structural changes of collagen in silicotic lungs of rats and patients. These included electron microscopy, circular dichroism and infrared spectroscopy studies of collagen fibers. The results indicated that fibers of silicotic collagen were shorter in length, smaller in diameter and decreased in α-helix content. The -Si-O-R- group and -OH group were found increased and -C-C- backbone shortened. The increase of -Si-O-R-group indicated that silica formed linking bridges between collagens which may be the cause of progressive enlargement of nodules 展开更多
关键词 SI In Studies on Structural Changes of Collagen in silicosis
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Preparation and Biocompatibility of Porous Poly(vinylalcohol)-Glycosaminoglycan-Collagen Scaffold 被引量:1
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作者 LI Qin-hua MO Xiao-hui 《Chinese Journal of Biomedical Engineering(English Edition)》 2013年第1期15-22,共8页
This paper aims to prepare a PVA-GAG-COL composite with polyvinyl alcohol (PVA), glycosaminoglycan (GAG) and collagen (COL) by the method of freeze drying and to investigate the feasibility as a tissue engineering sca... This paper aims to prepare a PVA-GAG-COL composite with polyvinyl alcohol (PVA), glycosaminoglycan (GAG) and collagen (COL) by the method of freeze drying and to investigate the feasibility as a tissue engineering scaffold for tissue or organ repairing. In this study, SEM was used to observe the morphology. Biocompatibility was tested by cell culture with the extracted fluid of composite materials. Different proportional scaffolds could be obtained with different concentrations and alcoholysis degree of PVA. Different proportional scaffolds also had different porous structures. SEM proved that large amount of porous structure could be formed. Biocompatibility test showed that the extracted fluid of composite materials was nontoxic, which could promote the adhesion and proliferation of the fibroblast. Fibroblast could grow on the scaffold normally.A porous scaffold for tissue engineering with high water content can be fabricated by PVA, GAG and COL, which has excellent cell biocompatibility. The porous structure shows potential in tissue engineering and cell culture. 展开更多
关键词 poly(vinylalcohol) glycosaminoglycan collagen porous composite scaffold biocompatibility
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Polyvinyl Alcohol-Collagen Composite with Glycosaminoglycan as Scaffolds for Tissue Engineering
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作者 LI Qin-hua MO Xiao-hui CHEN Jian-su 《Chinese Journal of Biomedical Engineering(English Edition)》 2008年第4期166-176,共11页
The aim of this study is to prepare a PVA-GAG-COL composite material by polyvinyl alcohol (PVA),glycosaminoglycan (GAG) and collagen (COL),and to investigate the feasibility of serving as a scaffold for tissue enginee... The aim of this study is to prepare a PVA-GAG-COL composite material by polyvinyl alcohol (PVA),glycosaminoglycan (GAG) and collagen (COL),and to investigate the feasibility of serving as a scaffold for tissue engineering. PVA was blended with various amounts of GAG and COL. Different proportional scaffolds could be obtained with different molecular weight and alcoholysis degree of PVA and different amounts of GAG,which exhibited high water content (60%-95%) and showed different inner configuration with swelling ratio (120%-620%). SEM proved that different composite materials had different porous structures. 展开更多
关键词 polyvinyl alcohol glycosaminoglycan COLLAGEN SCAFFOLD tissue engineering
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Pores Created by Laser Surface Modification of Poly(vinylalcohol)-Collagen with Glycosaminoglycan Scaffold for Cell Culture in Tissue Engineering
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作者 LI Qin-hua MO Xiao-hui LI Hui 《Chinese Journal of Biomedical Engineering(English Edition)》 2012年第1期22-28,共7页
A PVA-GAG-COL composite scaffold is fabricated by polyvinyl alcohol (PVA), glyeosaminoglycan (GAG) and collagen (COL). Laser surface modification technology is used to make holes on the surface of the scaffolds.... A PVA-GAG-COL composite scaffold is fabricated by polyvinyl alcohol (PVA), glyeosaminoglycan (GAG) and collagen (COL). Laser surface modification technology is used to make holes on the surface of the scaffolds. Inside and outside interconnection micro-porous structure is obtained. Bioeompatibility test of the scaffolds shows that PVA-GAG-COL scaffold can promote the adhesion and proliferation of the fibroblast. Also, fibroblast can grow normally on the scaffolds with pore diameter from 115 um to 255 um and pore distance from 500 um to 2000 um. PVA-GAG-COL scaffolds possess excellent cell biocompatibility. The porous structure is suitable for cell culture in tissue engineering. 展开更多
关键词 polyvinyl alcohol glycosaminoglycan COLLAGEN tissue engineering composite scaffold laser surface modification
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TNF-α and IL-1RA Polymorphisms and Silicosis Susceptibility in Chinese Workers Exposed to Silica Particles:A Case-Control Study 被引量:12
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作者 WANG Yong Wei LAN Ya Jia +2 位作者 YANG Yue Lin WANG De Jun KUANG Jie 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2012年第5期517-525,共9页
Abstract Objective To assess the association of TNF-α and IL-1RA SNPs with the risk of silicosis in Chinese workers exposed to silica particles. Methods Case-control study design was used to enroll 68 silicotic patie... Abstract Objective To assess the association of TNF-α and IL-1RA SNPs with the risk of silicosis in Chinese workers exposed to silica particles. Methods Case-control study design was used to enroll 68 silicotic patients induced by silica particles and 68 healthy workers matched for length of silica particle exposure as controls. Both cases and controls were from the same company in southwest China, and each of them was requested to complete a questionnaire. Blood samples were drawn for genomic DNA extraction from each participant. The genotyping of TNF-α (-238 and -308) and IL-1RA (+2018) was performed using polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) and SYBR green-based quantitative polymerase chain reaction {qPCR), respectively. Unconditional logistic regression model was used to estimate odds ratios (ORs) and their 95% confidential intervals (Cl) for SNPs. Results No significant differences were found between cases and controls in particles exposure length, body mass index (BMI), and status of smoking and alcohol consumption except for age (P=O.O01) and blood type (P=0.042). The frequencies of TNF-c((-238) and IL-1RA (+2018) genotypes in cases were significantly different from those in controls, (P=O.O01 and P=0.O02, respectively), while a borderline significant difference was found in the frequencies of TNF-α(-308) between cases and controls (P=0.063). The variants of three SNPs increased the risk of silicosis in the Chinese workers exposed to silica particles. The adjusted ORs of TNFα(-308), TNF-α(-238) and IL-1RA (+2018) were 2.8 (95% Ch 2.1-7.5), 20.9 (95% Ch 2.8-236.4) and 4.0 (95% CI: 2.6-10.2), respectively. Conclusion It is suggested that cytokine polymorphisms of TNF-ct (-238, -308) and IL-1RA (+2018) are associated with the risk of silicosis in the Chinese workers exposed to silica particles. Further independent studies on the interaction between SNPs and exposure to silica particles with a larger sample size are therefore warranted. 展开更多
关键词 TNF-α IL-1RA POLYMORPHISM silicosis Case-control study
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Quantitative detection and comparison of sulfate glycosaminoglycans content in extracellular matrix of in vitro cultured epiphyseal, articular and rib chondrocytes
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作者 陶凌晖 《外科研究与新技术》 2003年第2期97-97,共1页
Objective To establish a method for quantitative detection of the sulfate glycosaminoglycans ( GAG) content in extracellular matrix of in vitro cultured chondrocytes so as to evaluate the biological characteristics of... Objective To establish a method for quantitative detection of the sulfate glycosaminoglycans ( GAG) content in extracellular matrix of in vitro cultured chondrocytes so as to evaluate the biological characteristics of epiphyseal, articular and rib chondrocytes. Methods Sulfate GAG content in extracellular matrix of three chondrocytes was measured by the modified dimethylmethylene blue (DMB) method. The changes of the toluidine blue (TB) stain of chondrocytes were observed by light microscope. Results Primary chondrocytes had the highest content of sulfate GAG in the extracellular matrix, ie, epiphyseal chondrocytes reached ( 70. 12 ± 7. 72 )μg/cm2, articular chondrocytes (92.00 ± 10.15) μg/cm2 and rib chondrocytes (80.61 ± 11. 40) μg/cm2, respectively. On the third pasage chondrocytes, epiphyceal chondrocytes decreased to (53.27 ± 9. 50 ) μg/cm2, articular chondrocytes to (63.88 ± 11.92) μg/cm2 and rib chondrocytes to (58.94 ±8.21) μg/cm2, respectively. The change of TB in every passage 展开更多
关键词 in of Quantitative detection and comparison of sulfate glycosaminoglycans content in extracellular matrix of in vitro cultured epiphyseal
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Combined silicosis and mixed dust pneumoconiosis with rapid progression: A case report and literature review 被引量:4
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作者 Hee-Young Yoon Yookyung Kim +2 位作者 Heae Surng Park Chung-Won Kang Yon Ju Ryu 《World Journal of Clinical Cases》 SCIE 2018年第16期1164-1168,共5页
BACKGROUND Rapidly progressive pneumoconiosis(RPP) occasionally occurs in coal workers, particularly those with high exposure to silica. Here, we report the case of a 64-year-old male miller with RPP. CASE SUMMARY The... BACKGROUND Rapidly progressive pneumoconiosis(RPP) occasionally occurs in coal workers, particularly those with high exposure to silica. Here, we report the case of a 64-year-old male miller with RPP. CASE SUMMARY The patient had a persistent cough for one month and had been clinically diagnosed with pulmonary tuberculosis in 2011. He worked in a stone processing factory from the ages of 20 through 37 and has owned his own mill for the past 25 years. His chest radiograph showed significant increases in the size and number of lung nodules since his last follow-up in 2013. By percutaneous needle lung biopsy, the nodular lesions showed diffuse infiltration of phagocytic macrophages and birefringent crystals by polarizing microscopy. He was finally diagnosed with RPP of mixed dust pneumoconiosis combined with silicosis. CONCLUSION In this case, mixed dust pneumoconiosis with silicosis might be accelerated by persistent exposure to graindust from working in a mill environment. 展开更多
关键词 PATHOLOGY Case report PNEUMOCONIOSIS silicosis DUST OCCUPATIONAL diseases
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The Relationship between Polymorphisms of Interleukin-4 Gene and Silicosis 被引量:3
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作者 FANG Guo Feng FAN Xue Yun SHEN Fu Hai 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2011年第6期678-682,共5页
Objective To explore the relationship between polymorphisms of interleukin-4 (IL-4) gene (-33, +45, intron3, +429, +448) and the susceptibility of silicosis. Methods A case-control study was carried out. 101 si... Objective To explore the relationship between polymorphisms of interleukin-4 (IL-4) gene (-33, +45, intron3, +429, +448) and the susceptibility of silicosis. Methods A case-control study was carried out. 101 silicosis patients were selected as cases. As strictly matching, 121 of non silicosis workers were selected as the controls. The polymophisms of IL-4 (five locus) were detected by the method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques. Results The GA genotype in the IL-4+429 locus and the CC genotype in the IL-4+448 locus were found. The frequencies ofAA, GG and AG of IL-4+45 locus in the cases were 55.4%, 10.9%, and 33.7% and in the controls were 62.0%, 12.6%, and 26.4%. The differences between cases and controls were not significant. The frequencies of B1B1, B2B2, and B1B2 of intron3 VNTR locus in the cases were 73.3%, 1.0%, and 25.7% and in the controls were 68.6%, 1.7%, and 29.8%. The differences were not significant. The frequencies of TT, CC, and CT in -33 locus in the cases were 55.4%, 11.9%, and 32.7% and in the controls were 69.4%, 4.1%, and 26.4%. The differences were significant (P=0.034). Conclusion The relationship between genetic polymorphism of IL-4-33 site and silicosis has been found and -33TT is a protective genotype for silicosis. 展开更多
关键词 silicosis INTERLEUKIN-4 POLYMORPHISM SUSCEPTIBILITY
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Modified Glasgow Prognostic Score, and Neutrophil/lymphocyte and Platelet/lymphocyte Ratios in Different Stages of Silicosis 被引量:6
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作者 HE La Gu WU Peng Fei +7 位作者 PENG Yan Hua FENG Juan ZHONG Dai Ming ZHANG Gui Hua ZENG Lei ZHONG Yu Min WANG Xiao Ye YUAN Hua Min 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2019年第5期376-379,共4页
Until now, although comprehensive management strategies have improved treatment, there are no treatments to alleviate symptoms and slow disease progression[1]. In the past few decades, there has been increasing eviden... Until now, although comprehensive management strategies have improved treatment, there are no treatments to alleviate symptoms and slow disease progression[1]. In the past few decades, there has been increasing evidence that inflammation plays a very important role in silicosis. Injury-induced inflammation is an effective strategy to remove harmful stimuli and initiate a healing process. However, it might be harmful to the organism and result in a permanent disease state if the inflammation is prolonged[2]. 展开更多
关键词 silicosis PROGNOSTIC SCORE
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Serum neuron-specific enolase:A promising biomarker of silicosis 被引量:3
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作者 Hong-Bo Huang Jun-Ling Huang +4 位作者 Xiao-Ting Xu Kun-Bo Huang Yi-Jian Lin Jie-Bin Lin Xi-Bin Zhuang 《World Journal of Clinical Cases》 SCIE 2021年第5期1016-1025,共10页
BACKGROUND Silicosis is a type of chronic pulmonary fibrosis caused by long-term inhalation of silica dust particles.There has been no ideal biomarker for the diagnosis and differential diagnosis of silicosis until no... BACKGROUND Silicosis is a type of chronic pulmonary fibrosis caused by long-term inhalation of silica dust particles.There has been no ideal biomarker for the diagnosis and differential diagnosis of silicosis until now.Studies have found that elevated neuron-specific enolase(NSE)concentration in the serum of silicosis patients is helpful for diagnosis and severity assessment of the disease.However,the number of cases in these studies was not enough to arouse attention.AIM To investigate the clinical significance of serum NSE in the diagnosis and staging of silicosis.METHODS From January 2017 to June 2019,326 cases of silicosis confirmed in Quanzhou First Hospital Affiliated to Fujian Medical University were included in the silicosis group.A total of 328 healthy individuals or medical patients without silicosis were included in the control group.Serum NSE concentrations of all subjects were determined by electrochemical luminescence.RESULTS There were no significant differences in sex,age,smoking index and complications between the silicosis and control groups.The mean serum NSE concentration was 26.57±20.95 ng/mL in the silicosis group and 12.42±2.68 ng/mL in the control group.The difference between the two groups was significant(U=15187,P=0.000).Among the 326 patients with silicosis,103 had stage I silicosis,and the mean serum NSE concentration was 15.55±6.23 ng/mL.The mean serum NSE concentration was 21.85±12.05 ng/mL in 70 patients with stage II silicosis.The mean serum NSE concentration was 36.14±25.72 ng/mL in 153 patients with stage III silicosis.Kruskal-Wallis H test suggested that the difference in serum NSE concentration in silicosis patients in the three groups was significant(H=130.196,P=0.000).Receiver operating characteristic curve analysis indicated that the area under the curve was 0.858(95%confidence interval:0.828-0.888;P=0.000).When the NSE concentration was 15.82 ng/mL,the Jorden index was the largest,the sensitivity was 72%,and the specificity was 90%.CONCLUSION Serum NSE concentration may be a promising biomarker for the diagnosis and assessment of severity of silicosis. 展开更多
关键词 silicosis Neuron-specific enolase Receiver operating characteristic curve Disease stage BIOMARKER DIAGNOSIS
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Effects of respiratory rehabilitation training combined with Corbrin Capsule on the inflammatory response and pulmonary fibrosis in patients with silicosis complicated by COPD 被引量:1
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作者 Xiang-Jie Liu Hong-Yi Yao +4 位作者 Yan Tu Ying Shen Yan-Qiong Qin Chao Lei Ning-Xu Li 《Journal of Hainan Medical University》 2018年第4期51-54,共4页
Objective: To explore the effects of respiratory rehabilitation training combined with Corbrin Capsule on the inflammatory response and pulmonary fibrosis in patients with silicosis complicated by COPD. Methods: A tot... Objective: To explore the effects of respiratory rehabilitation training combined with Corbrin Capsule on the inflammatory response and pulmonary fibrosis in patients with silicosis complicated by COPD. Methods: A total of 80 patients with silicosis complicated by COPD who were treated in our hospital between January 2013 and December 2016 were selected as the research subjects and randomly divided into control group (n=40) and observation group (n=40). Control group were treated with Corbrin Capsule on the basis of routine treatment, and observation group were treated with respiratory rehabilitation training combined with Corbrin Capsule on the basis of routine treatment. The differences in serum levels of inflammatory factors and pulmonary fibrosis indexes were compared between the two groups before intervention, after 6 months of intervention and after 1 year of intervention. Results: Before intervention, there was no statistically significant difference in serum levels of inflammatory factors and pulmonary fibrosis indexes between the two groups. After 6 months of intervention and after 1 year of intervention, serum inflammatory factors IL-6, IL-8, IL-12, IL-17, IL-18 and MCP-1 levels of observation group were lower than those of control group;serum pulmonary fibrosis indexes PCⅢ, LN, HA, ICAM-1 and HO-1 contents were lower than those of control group. Conclusion: Respiratory rehabilitation training combined with Corbrin Capsule therapy can effectively reduce the systemic inflammatory response and inhibit the pulmonary fibrosis process in patients with silicosis complicated by COPD. 展开更多
关键词 silicosis COPD RESPIRATORY rehabilitation training Corbrin CAPSULE
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Natural Course of Silicosis in Dust-exposed Workers
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作者 杨海兵 杨磊 +1 位作者 张钧岳 陈镜琼 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2006年第2期257-260,共4页
To provide a scientific basis for determining the health surveillance period of dust-exposed workers, data of a retrospective cohort study was re-analyzed with emphasis on natural course of silicosis. 33640 workers ex... To provide a scientific basis for determining the health surveillance period of dust-exposed workers, data of a retrospective cohort study was re-analyzed with emphasis on natural course of silicosis. 33640 workers exposed to silica dust who were employed for at least 1 year from 1972 to 1974 in twenty Chinese mines or pottery factories were included as subjects, and were followed up till December 31, 1994. The cohort included subjects from 8 tungsten mines, 4 tin mines and 8 pottery factories. Our results showed that the mean latency of silicosis, for all the cases of the cohorts, was 22.9±9.8 y. 52.2 % of silicosis was diagnosed approximately 9.1±5.7 y after the dust exposure had ceased. The progression rates of silicosis from stage Ⅰ to Ⅱ and from stage Ⅱ to Ⅲ were 48.2 % and 18.5 %, and the duration was 4.1±0. 2 and 6.8±0.2 y, respectively. The survival times of silicosis stage Ⅰ , Ⅱ and Ⅲ, from the year of diagnosis to death, were 21.5, 15.8 and 6.8 years, respectively. There was 25 % of the silicosis patients whose survival time was beyond 33 y. The mean death age of all silicosis cases was 56.0 y. The death age inereased to 65.6 y in the middle of 1990s. Among dust-exposed workers, subjects who became suspected case (0^+ ) accounted for 15.0 %. 48.7 % of the suspected silicosis cases developed to silicosis, and the average year from the time of being suspected of the disease to the first stage of silicosis was 5.1y. The natural characteristics, as mentioned above, varied with different mines and factories. We are led to conclude that silicosis is chronic in nature, but progress quickly. As a serious occupational disease it significantly reduces the life span of exposed workers. The study of its natural history is of importance for the development of health surveillance criteria for dust-exposed workers. 展开更多
关键词 silicosis LATENCY retrospective cohort study health surveillance criteria
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