Numerical study on the cavity dynamics for vertical water entries of twin spheres

In this study, a three dimensional(3D) numerical model of six-degrees-of-freedom(6DOF) is applied to simulate the water entries of twin spheres side-by-side at different lateral distances and time intervals.The turbulence structure is described using the shear-stress transport k-ω(SST k-ω) model, and the volume of fluid(VOF) method is used to track the complex air-liquid interface. The motion of spheres during water entry is simulated using an independent overset grid. The numerical model is verified by comparing the cavity evolution results from simulations and experiments. Numerical results reveal that the time interval between the twin water entries evidently affects cavity expansion and contraction behaviors in the radial direction. However, this influence is significantly weakened by increasing the lateral distance between the two spheres. In synchronous water entries, pressure is reduced on the midline of two cavities during surface closure, which is directly related to the cavity volume. The evolution of vortexes inside the two cavities is analyzed using a velocity vector field, which is affected by the lateral distance and time interval of water entries.

Roles of host proteases in the entry of SARS-CoV-2

The spike protein(S)of SARS-CoV-2 is responsible for viral attachment and entry,thus a major factor for host suscep-tibility,tissue tropism,virulence and pathogenicity.The S is divided with S1 and S2 region,and the S1 contains the receptor-binding domain(RBD),while the S2 contains the hydrophobic fusion domain for the entry into the host cell.Numerous host proteases have been implicated in the activation of SARS-CoV-2 S through various c leavage sites.In this article,we review host proteases including furin,trypsin,transmembrane protease serine 2(TMPRSS2)and cathepsins in the activation of SARS-CoV-2 S.Many betacoronaviruses including SARS-CoV-2 have polybasic residues at the S1/S2 site which is subjected to the cleavage by furin.The S1/S2 cleavage facilitates more assessable RBD to the receptor ACE2,and the binding triggers further conformational changes and exposure of the S2'site to proteases such as type Il transmembrane serine proteases(TTPRs)including TMPRSS2.In the presence of TMPRSS2 on the target cells,SARS-CoV-2 can utilize a direct entry route by fusion of the viral envelope to the cellular membrane.In the absence of TMPRSS2,SARS-CoV-2 enter target cells via endosomes where multiple cathepsins cleave the S for the successful entry.Additional host proteases involved in the cleavage of the S were discussed.This article also includes roles of 3C-like protease inhibitors which have inhibitory activity against cathepsin L in the entry of SARS-CoV-2,and discussed the dual roles of such inhibitors in virus replication.

Polypyrimidine Tract-Binding Protein Enhances Zika Virus Translation by Binding to the 5'UTR of Internal Ribosomal Entry Site

Objectives To identify the 5'untranslated region of Zika virus(ZIKV 5'UTR)RNA-binding proteins and to investigate the impact of the binding protein on the activity of internal ribosomal entry site(IRES)located in ZIKV 5'UTR and virus production.Methods Interacting proteins in U251 cells were captured using tRSA-tagged ZIKV 5'UTR RNA and tRSA-ZIKV 5'UTR RNA-binding proteins were visualized by SDS-PAGE silver staining,Subsequently,liquid chromatographytandem mass spectrometry(LC-MS/MS),bioinformatics analysis,and Western blot were used to identify the candidate proteins binding to ZIKV 5'UTR.Dicistronic expression assay and plaque forming assay were performed to analyze the effect of the binding protein on ZIKV IRES activity and ZIKV production,respecitvely.Results tRSA RNA pull-down assay,LC-MS/MS,and Western blot analysis showed that polypyrimidine tractbinding protein(PTB)bound to the ZIKV 5'UTR.Furthermore,dual luciferase reporter assay revealed that overexpression of PTB significantly enhanced the IRES activity of ZIKV(t=10.220,P<0.001),while PTB knockdown had the opposite effect(t=4.897,P<0.01).Additionally,virus plaque forming assay demonstrated that up-regulation of PTB expression significantly enhanced viral titer(t=6.400,P<0.01),whereas reducing PTB expression level weakened virus infectivity(t=5.055,P<0.01).Conclusion PTB positively interacts with the ZIKV 5'UTR and enhances IRES activity and virus production.

Ground response and failure mechanism of gob-side entry by roof cutting with hard main roof

This study is the result of long-term efforts of the authors’team to assess ground response of gob-side entry by roof cutting(GSERC)with hard main roof,aiming at scientific control for GSERC deformation.A comprehensive field measurement program was conducted to determine entry deformation,roof fracture zone,and anchor bolt(cable)loading.The results indicate that GSERC deformation presents asymmetric characteristics.The maximum convergence near roof cutting side is 458 mm during the primary use process and 1120 mm during the secondary reuse process.The entry deformation is closely associated with the primary development stage,primary use stage,and secondary reuse stage.The key block movement of roof cutting structure,a complex stress environment,and a mismatch in the supporting design scheme are the failure mechanism of GSERC.A controlling ideology for mining states,including regional and stage divisions,was proposed.Both dynamic and permanent support schemes have been implemented in the field.Engineering practice results indicate that the new support scheme can efficiently ensure long-term entry safety and could be a reliable approach for other engineering practices.

Application of Thermodynamic Database to Corrosion of ZrO_(2) Containing Submerged Entry Nozzle in Steel Continuous Casting Process

The CALPHAD thermodynamic databases are very useful to analyze the complex chemical reactions happening in high temperature material process.The FactSage thermodynamic database can be used to calculate complex phase diagrams and equilibrium phases involving refractories in industrial process.In this study,the FactSage thermodynamic database relevant to ZrO_(2)-based refractories was reviewed and the application of the database to understanding the corrosion of continuous casting nozzle refractories in steelmaking was presented.

Epidata Entry-Epidata Analysis软件联用在医院处方分析评价中的应用

目的探讨不合格处方分析评价的新技术、新方法。方法运用Epidata Entry软件进行数据录入,通过编写check文件,对录入数据和流程进行控制,联用Epidata Analysis软件对不合格处方进行分析。结果 Epidata Entry软件便于不合格处方的录入和管理,Epidata Analysis软件便于不合格处方的分析,两软件联用可实现不合格处方数字化管理,方法可靠。结论该方法应用于医院处方分析评价,具有简单、易学、方便、实用、几乎不需任何费用等优点。

Comparison and Analysis on Animal and Plant Quarantine of Entry Mails among China, Australia and New Zealand

With the significant increase of the number of entry mails in China, mail inspection has become more and more challenge. Australia and New Zealand are the two countries recognized as the ones with the most stringent inspection and quarantine measures in the world. The mail inspection work in China, Australia and New Zealand was compared on the aspects of mail quarantine system, inspection, quarantine treatment and punishment, based on which something worth learning and referring could be found and some suggestions were put forward, with the aim to provide theoretical basis for the animal and plant quarantine of entry mails.

Clogging behavior of submerged entry nozzles for Ti-bearing IF steel

The nozzle clogging behavior of Ti-bearing IF steel was studied by metallographic analysis,scanning electron microscopy（SEM）, energy dispersive spectroscopy（EDS）,and X-ray diffraction（XRD）.According to the experimental results,nozzle clogging primarily appears three layers.There are a lot of large-sized iron particles in the inner layer and mainly slag phase in the middle and outer layers.The principal clog constituents of the inner layer are loose alumina cluster inclusions and granular shaped alumina inclusions,containing iron particles. The clog constituents of the middle layer are mainly dendrite alumina inclusions.The primary phases existing in nozzle clogging are FeO·TiO2 and FeO·Al2O3 besidesα-Al2O3 and a-Fe.The FeO·TiO2 phases among the deposits adhere the deposits together firmly enough to lead to the inferior castability of Ti-bearing ultra low carbon steel compared with that of Ti-free low carbon Al-killed steel.

Rock burst mechanism analysis in an advanced segment of gob-side entry under different dip angles of the seam and prevention technology

In order to investigate the frequent occurrences of rock burst in gob-side entry during the mining process of the mining zone No. 7, the mechanical model of main roof of fully-mechanized caving mining before breaking was established by the Winkler foundation beam theory, and the stress evolution law of surrounding rock with different dip angles of the seam during the mining process was analyzed by using FLAC3 D. The results show that: with the dip angle changing from 45° to 0°, the solid-coal side of gobside entry begins to form an L-shaped stress concentration zone at a dip angle of 30°, and the stress concentration degree goes to higher and higher levels. However, the stress concentration degree of the coalpillar side goes to lower and lower levels; the influence range and peak stress of the abutment at the lateral strata of adjacent gob increase with dip angle decreasing and reach a maximum value at a dip angle of 0°, but the tailgate is not affected; the abutment pressure superposition of two adjacent gobs leads to stress concentration further enhancing in both sides of gob-side entry. With the influence of strong mining disturbance, rock burst is easily induced by dynamic and static combined load in the advanced segment of gob-side entry. To achieve stability control similar to that in the roadway, the key control strategy is to reinforce surrounding rock and unload both sides. Accordingly, the large-diameter drilling and high-pressure water injection combined unloading and reinforced support cooperative control technology was proposed and applied in field test. The results of Electromagnetic Emission(EME) and field observation showed that unloading and surrounding rock control effect was obvious.

UNDERWATER ACOUSTICS AND CAVITATING FLOW OF WATER ENTRY

The fluid mechanics of water entry is studied through investigating the underwater acoustics and the supercavitation.Underwater acoustic signals in water entry are extensively measured at about 30 different positions by using a PVDF needle hydrophone.From the measurements we obtain (1)the primary shock wave caused by the impact of the blunt body on free surface;(2)the vapor pressure inside the cavity;(3)the secondary shock wave caused by pulling away of the cavity from free surface;and so on.The supercavitation induced by the blunt body is observed by using a digital high-speed video camera as well as the single shot photography.The periodic and 3 dimensional motion of the supercavitation is revealed.The experiment is carried out at room temperature.

Integrated Entry Guidance for Reusable Launch Vehicle

A method for the implementation of integrated three-degree-of-freedom constrained entry guidance for reusable launch vehicle is presented. Given any feasible entry conditions, terminal area energy management interface conditions, and the reference trajectory generated onboard then, the method can generate a longitudinal guidance profile rapidly, featuring linear quadratic regular method and a proportional-integral-derivative tracking law with time-varying gains, which satisfies all the entry corridor constraints and meets the requirements with high precision. Afterwards, by utilizing special features of crossrange parameter, establishing bank-reversal corridor, and determining bank-reversals according to dynamically adjusted method, the algorithm enables the lateral entry guidance system to fly a wide range of missions and provides reliable and good performance in the presence of significant aerodynamic modeling uncertainty. Fast trajectory guidance profiles and simulations with a reusable launch vehicle model for various missions and aerodynamic uncertainties are presented to demonstrate the capacity and reliability of this method.

HYDRODYNAMIC BEHAVIOR OF AN UNDERWATER MOVING BODY AFTER WATER ENTRY

An experimental study was conducted to investigate the water entry phenomenon. A facility was designed to carry out the tests with the entry veloci- ties of around 352 m/s. Visualization, pressure ineasurement, velocity measurement and underwater impact test were performed to investigate the hydroballistic behav- ior of the underwater moving body, the underwater flow field, the supercavitation, etc.. This study shows that the motion of a high-speed underwater body is strongly three-dimensional and chaotic. Furthermore, it is found that the distribution of the trajectory deflection of the underwater projectile depends on the depth of water. It is also found by measuring the deformation on a witness plate submerged in water, that the impact energy of an underwater projectile is reduced as it penetrates deeper into water.

Surrounding rock control of gob-side entry driving with narrow coal pillar and roadway side sealing technology in Yangliu Coal Mine

Gob-side entry driving can increase coal recovery ratio, and it is implied in many coal mines. Based on geological condition of 10416 working face tailentry in Yangliu Coal Mine, the surrounding rock deformation characteristics of gob-side entry driving with narrow coal pillar is analysed, reasonable size of coal pillar and reasonable roadway excavation time after mining are achieved. Surrounding rock control technology and effective roadway side sealing technology are proposed and are taken into field practice. The results showed that a safer and more efficient mining of working face can be achieved. In addition, results of this paper also have important theoretical significance and valuable reference for surrounding rock control technology of gob-side entry driving with narrow coal pillar under special geological condition.

How hepatitis C virus invades hepatocytes: The mystery of viral entry

Hepatitis C virus(HCV)infection is a global health problem,with an estimated 170 million people being chronically infected.HCV cell entry is a complex multi-step process,involving several cellular factors that trigger virus uptake into the hepatocytes.The high-density lipoprotein receptor scavenger receptor class B type I,tetraspanin CD81,tight junction protein claudin-1,and occludin are the main receptors that mediate the initial step of HCV infection.In addition,the virus uses cell receptor tyrosine kinases as entry regulators,such as epidermal growth factor receptor and ephrin receptor A2.This review summarizes the current understanding about how cell surface molecules are involved in HCV attachment,internalization,and membrane fusion,and how host cell kinases regulate virus entry.The advances of the potential antiviral agents targeting this process are introduced.

New insights into store-independent Ca^(2+) entry: secretory pathway calcium ATPase 2 in normal physiology and cancer

Recent studies in secretory pathway calcium ATPases （SPCA） revealed novel functions of SPCA2 in interacting with store-operated Ca2＋ channel Oral I and inducing Ca2＋ influx at the cell surface. Importantly, SPCA2-mediated Ca2＋ signaling is uncoupled from its conventional role of Ca2＋-ATPase and independent of store-operated Ca2＋ signaling pathway. SPCA2-induced store-independent Ca2＋ entry （SICE） plays essential roles in many important physiological processes, while unbalanced SICE leads to enhanced cell proliferation and tumorigenesis. Finally, we have summarized the clinical implication of SICE in oral cancer prognosis and treatment. Inhibition of SICE may be a new target for the development of cancer therapeutics.

The Form of Literary Translation——“Foregrounding Translation” and the Translator's Poetic Entry

From the epistemological perspective translating sense is to pursue truth,while translating flavor is to pursue beauty.Rational knowledge is aimed at"fidelity";perceptive knowledge the"beauty".Literary language approaches fidelity because of beauty while non-literary language approaches beauty because of its fidelity.

Deformation characteristics and reinforcement technology for entry subjected to mining-induced stresses

The entry at Zhangcun coal mine in Lu＇an coal mining area in Shanxi Province suffered from severe mining-induced stresses with the heading face driven oppositely to an adjacent working face. In this paper, the characteristics of deformation and failure of the entry were investigated in terms of the tempo-spatial relations between heading and working faces through field study and numerical modeling. The three-dimensional （3D） finite difference models were built to investigate stresses, displacements and damages in the surrounding rocks of the entry and the working face. The field study includes selection of reinforcing methods and materials, design parameters, and determination of cable prestress. The monitoring data of entry deformation and stress along the cables during every stage were presented. The state of the reinforced entry was evaluated based on the monitoring data. The results demonstrate that before the heading face of the entry crosses the adjacent working face, the influence of advanced abutment pressure caused by adjacent working face upon the entry is not significant. After they cross each other, however, the lateral abutment pressure will have an evident impact on the entry. The displacement rate of the entry will be greatly increased and reaches a certain value within a certain distance between the heading face and the working face. Then, it will increase again with the presence of secondary mining-induced pressure on the entry when the present working face advances. The fully-grouted cable with short length, high strength and high prestress is an effective way to reinforce the entry suffering from severe mining-induced stresses, which greatly reduces the displacement and failure possibility of the entry. Finally, the principles and recommendations for reinforcing design of entries suffering from severe mining-induced stresses were proposed according to field study, numerical modeling and experiences from other coal mines. Problems encountered in field study and suggestions for reinforcement were also discussed.

Control mechanism and technique of floor heave with reinforcing solid coal side and floor corner in gob-side coal entry retaining

Floor heave is the most common convergence in gob-side entry retaining.The paper analyzes the form,process and characteristics of gob-side entry retaining with the comprehensive methods of theoretical analysis,numerical simulation and the field trial.Research results present that bending and folding floor heave is the main factor in the stage of the first panel mining;squeezing and fluidity floor heave plays a great role in the stable stage of gob-side entry retaining;the combination of the former two factors affects mainly the stage of the second mining ahead;abutment pressure is a fundamental contribution to the serious floor heave of gob-side entry retaining,and sides corners of solid coal body are key part in the case of floor heave controlling of gob-side entry retaining.Floor heave of gob-side entry retaining can be significantly controlled by reinforcing sides and corners of solid coal body,and influence rules on the floor heave of gob side entry retaining of sides supporting strength and the bottom bolt orientation in solid coal side are obtained.Research results have been successfully applied in gob-side entry retaining of G20-F23070 face haulage roadway in #2 coal mine of Pingmei Group,and the field observation shows that the proposed technique is an effective way in controlling the floor heave of gob-side entry retaining.

An Innovative Non-Pillar Coal-Mining Technology with Automatically Formed Entry: A Case Study

A non-pillar coal-mining technology with an automatically formed entry is proposed,which reduces the waste of coal resources and the underground entry drivage workload.Three key techniques in this technology cooperate to achieve automatic formation and retaining of the gob-side entry,and to realize nonpillar mining.Constant-resistance large deformation(CRLD)support ensures the stability of the entry roof;directional presplitting blasting(DPB)separates the entry roof and the gob roof;and a blockinggangue support system(BGSS)integrates the caved rock material as an effective entry rib.An industrial test was conducted to verify the engineering effects of these key techniques.The field application results showed that the retained entry was under the pressure-relief zone due to the broken-expansion nature of the caved rock mass within the DPB height.After going through a provisional dynamic pressure-bearing zone,the retained entry entered the stability zone.The final stable entry meets the requirements of safety and production.The research results demonstrate the good engineering applicability of this technology.By taking the framework of the technology design principles into consideration and adjusting the measures according to different site conditions,it is expected that the proposed non-pillar coal-mining technology can be popularized on a large scale.

Thermochemical ablation of spherical cone during re-entry

Presents the use of the similar transform and potential theory for calculation of the bypass flow factor and pressure gradient and the analysis of the influence of bypass flow factor and pressure gradient on heat transfer is analyzed, and the distribution of nose cone ablation obtained by combining the controlling equations of boundary layer, the compatible relation of interface and the heat conduction of interior.