New Diterpenes from Cultures of the Fungus Engleromyces goetzii and Their CETP Inhibitory Activity

One new cleistanthane-type diterpene named engleromycenolic acid A(1),one new rosane-type diterpene named engleromycenolic acid B(2)and one new natural rosane-type diterpene,engleromycenol(3),along with three known rosanetype diterpenes,rosololactone(4),rosenonolactone(5)and 7-deoxyrosenonolactone(6)were isolated from cultures of the fungus Engleromyces goetzii,where it naturally grows on Alpine bamboo culms.The new compounds were elucidated based on their spectroscopic data.In addition,compounds 1–6 were evaluated for their cholesterol ester transfer protein(CETP)inhibition activity.This paper reports the isolation,structural elucidation,and CETP inhibition activity of these compounds.

Identification and Cytotoxic Activities of Two New Trichothecenes and a New Cuparane-Type Sesquiterpenoid from the Cultures of the Mushroom Engleromyces goetzii

Engleromyces goetzii is a traditional medicinal mushroom that is widely used to treat infection,inflammation and cancer in Tibet,Sichuan and Yunnan provinces of China.Two new trichothecenes,engleromycones A and B(1 and 2),one new cuparane-type sesquiterpenoid named infuscol F(11),eight known trichothecene analogs,sambucinol(3),3-deoxysambucinol(4),trichothecolone(5),trichodermol(6),8-deoxytrichothecin(7),trichothecin(8),trichothecinol B(9)and trichothecinol A(10),and one known cyclopentanoid sesquiterpene cyclonerodiol(12)were isolated from the cultures of E.goetzii.The new compounds were elucidated through spectroscopic analyses.The anticancer effects of trichothecenes 1–10 were examined in the HL-60,SMMC-7721,A549,MCF-7,and SW-480 human cancer cell lines using an MTT assay.Trichothecinol A(10)significantly inhibited the growth of MCF-7 cells,with an IC50 value of 0.006 lM,which was comparable to the cytotoxic activity of the positive control,paclitaxel,indicating that trichothecinol A(10)represents a potential anticancer agent.