Insights into microbiota community dynamics and flavor development mechanism during golden pomfret(Trachinotus ovatus)fermentation based on single-molecule real-time sequencing and molecular networking analysis

Popular fermented golden pomfret(Trachinotus ovatus)is prepared via spontaneous fermentation;however,the mechanisms underlying the regulation of its flavor development remain unclear.This study shows the roles of the complex microbiota and the dynamic changes in microbial community and flavor compounds during fish fermentation.Single-molecule real-time sequencing and molecular networking analysis revealed the correlations among different microbial genera and the relationships between microbial taxa and volatile compounds.Mechanisms underlying flavor development were also elucidated via KEGG based functional annotations.Clostridium,Shewanella,and Staphylococcus were the dominant microbial genera.Forty-nine volatile compounds were detected in the fermented fish samples,with thirteen identified as characteristic volatile compounds(ROAV>1).Volatile profiles resulted from the interactions among the microorganisms and derived enzymes,with the main metabolic pathways being amino acid biosynthesis/metabolism,carbon metabolism,and glycolysis/gluconeogenesis.This study demonstrated the approaches for distinguishing key microbiota associated with volatile compounds and monitoring the industrial production of high-quality fermented fish products.

Highly Accurate Golden Section Search Algorithms and Fictitious Time Integration Method for Solving Nonlinear Eigenvalue Problems

This study sets up two new merit functions,which are minimized for the detection of real eigenvalue and complex eigenvalue to address nonlinear eigenvalue problems.For each eigen-parameter the vector variable is solved from a nonhomogeneous linear system obtained by reducing the number of eigen-equation one less,where one of the nonzero components of the eigenvector is normalized to the unit and moves the column containing that component to the right-hand side as a nonzero input vector.1D and 2D golden section search algorithms are employed to minimize the merit functions to locate real and complex eigenvalues.Simultaneously,the real and complex eigenvectors can be computed very accurately.A simpler approach to the nonlinear eigenvalue problems is proposed,which implements a normalization condition for the uniqueness of the eigenvector into the eigenequation directly.The real eigenvalues can be computed by the fictitious time integration method(FTIM),which saves computational costs compared to the one-dimensional golden section search algorithm(1D GSSA).The simpler method is also combined with the Newton iterationmethod,which is convergent very fast.All the proposed methods are easily programmed to compute the eigenvalue and eigenvector with high accuracy and efficiency.

A Golden Journey: Celebrating 50 Years of China-Malaysia Trade and Economic Cooperation

As we approach the golden jubilee of Malaysia-China diplomatic relations in May 2024,it is both timely and essential to reflect on the journey that has shaped one of the most dynamic and influential economic partnerships in Asia.This 50-year saga is not merely a story of diplomatic ties;it is a testament to enduring friendship,strategic cooperation and mutual growth that have significantly impacted both nations and the wider region.The relationship between Malaysia and China,which formally began in 1974,was groundbreaking in many respects.It symbolized not only Malaysia’s commitment to a forward-looking foreign policy but also China’s expanding engagement with Southeast Asia.Over the decades,this relationship has evolved from tentative initial contact into a robust,multifaceted partnership.

Translation of Culture-loaded Words in The Golden Cangue under the Perspective of Reception Aesthetics

Good translations play a very important role in cultural exchange.The idea of reception aesthetics think the reader as the center in translation process.This paper will study the translation of culture-loaded words from the perspective of reception aesthetics.It is divided into five parts.The first part mainly introduces the background and theoretical foundation of this paper,the second part introduces the writer’s works,the third part gives examples to analyze the culture-loaded words appearing in The Golden Cangue,the fourth part gives examples of the translation methods,and the fifth part is the conclusion.It aims to provide a reference for the English translation of culture-loaded words.

Litter Productivity and Nutrient Return Characteristics of Three Typical Forest Stands in Golden Mountain

Objective: The paper aims to analyze the dynamic characteristics of litter production and nutrient return of the forest ecosystems in subtropical areas, and provide a theoretical basis for the nutrient cycling study in southwest Hubei Province and carbon sink function of the whole forest ecosystem. Methods: Three typical forest stands (Chinese fir plantation, Cryptomeria fortunei plantation and evergreen and deciduous broad-leaved mixed forest) in Golden Mountain Forest Farm in southwest Hubei Province were investigated and monitored continuously for the litter types and productivity and nutrient return. Results: The annual litter productivity of the three forest stands ranged from 161.77 to 396.26 kg·hm-2;Litters of branches, leaves and reproductive organs accounted for 14.14% - 20.85%, 33.26% - 78.33%, 7.52% - 42.18% of the total, respectively;The litter productivity and total litter productivity of each composition in the three forest stands show unimodal or bimodal changes over months, and the total litter productivity reached the highest value in January, April and October respectively. For different nutrient contents of the three forest stands, the common feature is C > N. The order of nutrient return amount from greatest to least is evergreen and deciduous broad-leaved mixed forest, Cryptomeria fortunei plantation and Chinese fir plantation. For different nutrient return amounts, the common feature is C > N, and the nutrient return amounts are 76.51-180.69 kg·hm-2 and 2.3 - 5.71 kg·hm-2 respectively. Conclusion: The annual litter productivity and nutrient return amount of the evergreen and deciduous broad-leaved mixed forest are the highest among the three forest stands. Therefore, protecting the evergreen and deciduous broad-leaved mixed forest and studying the litter changes of Chinese fir plantation and Cryptomeria fortunei plantation are of far-reaching significance for the development of sustainable forest management in this region and the further improvement of the carbon sequestration function of the whole forest ecosystem.

Exploring Corporate Tax Compliance in the Era of Digital Tax Collection and Administration:Insights from the“Golden Tax Phase Four”Project

This article primarily examines the current state of tax collection and management,alongside other associated issues.It integrates insights from China’s“14th Five-Year Plan”and anticipates the imminent implementation of the“Golden Tax Phase Four.”With this backdrop,the article offers recommendations for advancing the reform of the tax collection and management system,fostering the development of intelligent taxation,and accelerating the modernization of tax collection and management in China.

基于Golden Gate高效构建psiCHECK双荧光素酶报告基因的方法及应用

目的 基于Golden Gate技术构建psiCHECK-CcdB重组双荧光素酶报告载体,并探讨其能否用于RNA干扰(RNA interference, RNAi)药物筛选。方法 通过聚合酶链反应(polymerase chain reaction,PCR)扩增CcdB致死基因、氯霉素基因与Golden Gate组装所需的BsmBI酶切位点,将其克隆至psiCHECK-2载体,构建出重组载体psiCHECK-CcdB。利用2种大肠杆菌DB3.1与DH5α对该载体的致死效率进行检测,选用1 387 bp大小的外源片段克隆至psiCHECK-CcdB载体,检测其连接效率,并验证重组载体能否发挥双荧光素酶报告系统功能,监测目的基因的表达变化。结果 菌落PCR以及测序鉴定psiCHECK-CcdB重组质粒构建成功,该质粒可有效致死无法耐受CcdB毒素的大肠杆菌DH5α菌株,在DB3.1菌株中正常生长。采用Golden Gate的方法可将外源片段简单快速克隆至psiCHECKCcdB载体中,连接效率显著提高且低背景克隆。通过测定双荧光素酶表达强度,证实了小干扰RNA(small interfering RNA,siRNA)靶向性结合目的基因可显著下调荧光素酶的表达,成功发挥双荧光素酶报告基因功能。结论 本研究成功构建了psiCHECK-CcdB重组双荧光素酶报告载体,应用Golden Gate组装技术,简化了实验操作流程,降低了实验成本,具备较高的阳性克隆率与多片段一次性组装的潜力,在RNAi药物筛选领域应用前景广阔。

Tao and Golden Ratio: A Scientific View of Contemporary Acupunctural Principles through Geometry

It is universally accepted that the philosophy of the Tao is the essence that animates the Chinese Cosmogony. Therefore, in last decades I have tried to consolidate its scientific background, looking for solid explanations through Exact Sciences (“Between Heaven and Earth” Scientific Basis of the Action of Shao Yin: Lightning’s Physical-Mathematical Analysis”;“Is Traditional Chinese Medicine Definitely an Exact Science?”;“Euclidean Geometry and Traditional Chinese Medicine: Diving into the Real Origin of the Five Elements”;“Solitons: A Cutting-Edge Scientific Proposal Explaining the Mechanisms of Acupuntural Action”) Currently, research on Chinese medicine leads us—with Dr. Erica Arakaki, collaborator and assistant—to verify through a profound bibliographic review the application of Fibonacci’s Golden Ratio in the constitution of T’ai Ji Tu, adding yet more substance to the hypothesis of Acupuncture and demonstrating how said Chinese Ancient Science is not only an empirical knowledge but a wisdom derived from the most ancient exact science: Geometry.

Novel Lipase from Golden Pompano(Trachinotus ovatus)Viscera:Purification,Characterization,and Application in the Concentrating of n-3 Polyunsaturated Fatty Acids

Lipases have been widely applied in a variety of industrial fields,such as food,pharmaceuticals,biofuels,and biotechnology.Recent years have witnessed a great interest in modifying lipids for the production of triacylglycerols enriched with n-3 polyunsaturated fatty acids(PUFAs).Here,a novel salt-tolerant,organic solvent-stable,and bile salt-activated lipase was purified from golden pompano(Trachinotus ovatus)viscera,which was named as golden pompano lipase(GPL).GPL had a specific activity of 57.2U mg^(-1)with an estimated molecular weight of 14 k Da,exhibited optimal activity at 40℃a nd pH 8.0,and showed K_(m)and V_(max)of 40.16μmol L^(-1)and 769.23μmol L^(-1)min^(-1),respectively.GPL activity was enhanced by Mn^(2+)and sodium deoxycholate.It was active in organic solvents,including methanol,ethanol,chloroform,and hexane.GPL also showed a good salinity tolerance of up to 1 mol L^(-1).n-3PUFA enrichment in the glyceride fraction of golden pompano oil was performed by GPL-catalyzed hydrolysis and yielded a total PUFA concentration of 56.99%.EPA,DHA,and DPA were enriched by 10.4-,3.2-,and 1.8-fold of their initial levels,respectively.This study recognized the industrial applicability of GPL to prepare enriched C_(20-22)n-3 PUFA.

Market capitalization shock effects on open innovation models in e‑commerce:golden cut q‑rung orthopair fuzzy multicriteria decision‑making analysis

This research paper analyzes revenue trends in e-commerce,a sector with an annual sales volume of more than 340 billion dollars.The article evaluates,despite a scarcity of data,the effects on e-commerce development of the ubiquitous lockdowns and restriction measures introduced by most countries during the pandemic period.The analysis covers monthly data from January 1996 to February 2021.The research paper analyzes relative changes in the original time series through the autocorrelation function.The objects of this analysis are Amazon and Alibaba,as they are benchmarks in the e-commerce industry.This paper tests the shock effect on the e-commerce companies Alibaba in China and Amazon in the USA,concluding that it is weaker for companies with small market capitalizations.As a result,the effect on estimated e-trade volume in the USA was approximately 35%in 2020.Another evaluation considers fuzzy decision-making methodology.For this purpose,balanced scorecard-based open financial innovation models for the e-commerce industry are weighted with multistepwise weight assessment ratio analysis based on q-rung orthopair fuzzy sets and the golden cut.Within this framework,a detailed analysis of competitors should be made.The paper proves that this situation positively affects the development of successful financial innovation models for the e-commerce industry.Therefore,it may be possible to attract greater attention from e-commerce companies for these financial innovation products.

Optimization of Chiller Loading Problem Using Improved Golden Jackal Optimization Algorithm Leads to Reduction in Energy Consumption

This paper proposes a modified golden jackal optimization(IGJO)algorithm to solve the OCL(which stands for optimal cooling load)problem to minimize energy consumption.In this algorithm,many tools have been developed,such as numerical visualization,local field method,competitive selectionmethod,and iterative strategy.The IGJO algorithm is used to improve the research capabilities of the algorithm in terms of global tuning and rotation speed.In order to fully utilize the effectiveness of the proposed algorithm,three famous examples of OCL problems in basic ventilation systems were studied and compared with some previously published works.The results show that the IGJO algorithm can find solutions equal to or better than other methods.Underpinning these studies is the need to reduce energy consumption in air conditioning systems,which is a critical business and environmental decision.The Optimal Chiller Load(OCL)problem is well-known in the industry.It is the best method of operation for the refrigeration plant to satisfy the requirement of cooling.In order to solve the OCL problem,an improved Golden Jackal optimization algorithm(IGJO)was proposed.The IGJO algorithm consists of a number of parts to improve the global optimization and rotation speed.These studies are intended to address more effectively the issue of OCL,which results in energy savings in air-conditioning systems.The performance of the proposed IGJO algorithm is evaluated,and the results are compared with the results of three known OCL problems in the ventilation system.The results indicate that the IGJO method has the same or better optimization ability as other methods and can improve the energy efficiency of the system’s cold air.

Online and Offline Integrated Teaching of Histology and Embryology Based on Concept of "Golden Course"

Taking construction of the online and offline integrated first-class undergraduate curriculum teaching modes of Histology and Embryology in Guangxi as an opportunity,under the guidance of student-centered teaching concept,efforts were made to innovate online and offline integrated teaching mode to overcome the shortcomings and dilemma of traditional Histology and Embryology teaching,with attention paid to the competence education in students including schematic knowledge,professional techniques,analytical thinking,and ideological and political theories,which would be of great significance for the cultivation of high-quality professionals specialized in traditional Chinese medicine.

Sticking to the Original Commitment to Develop the Sea Cucumber Business——Exclusive Interview with Chan Waili,Founder and Chairman of Golden Row Trading Limited&Sea Cucumber Palace International Limited

From going overseas to make a living,to starting his own business from scratch,to engaging in the sea cucumber industry and improving quality standards,in order to enable Chinese people to enjoy high-quality sea cucumber products,Chan Waili,the founder and Chairman of Golden Row Trading Limited and Sea Cucumber Palace International Limited,has continually worked hard.

Towards a Happy Life Through the Golden Bridge on Silk Road

Over the years,the Belt and Road cooperation has strengthened business and cultural ties between China and Africa.Eight years ago,a Ugandan girl named Rose joined a Chinese company in her home country and got in a cross-national romantic relationship.After marrying a Chinese husband,she moved to China and has become a worldrenowned"Chinese Agricultural Maker"who shares her happy life in China and Africa with over 20 million followers on social media.

The Golden Ratio Theorem: A Framework for Interchangeability and Self-Similarity in Complex Systems

The Golden Ratio Theorem, deeply rooted in fractal mathematics, presents a pioneering perspective on deciphering complex systems. It draws a profound connection between the principles of interchangeability, self-similarity, and the mathematical elegance of the Golden Ratio. This research unravels a unique methodological paradigm, emphasizing the omnipresence of the Golden Ratio in shaping system dynamics. The novelty of this study stems from its detailed exposition of self-similarity and interchangeability, transforming them from mere abstract notions into actionable, concrete insights. By highlighting the fractal nature of the Golden Ratio, the implications of these revelations become far-reaching, heralding new avenues for both theoretical advancements and pragmatic applications across a spectrum of scientific disciplines.

The Most Irrational Number that Shows up Everywhere: The Golden Ratio

Since the time of the ancient Greeks, humans have been aware of this mathematical idea. Golden ratio is an irrational number that is symbolized by the Greek numeral phi (φ). One can find this ratio everywhere. It is in nature, art, architecture, human body, etc. But this symbolism can result in a strong connection with mathematical nature. In this paper we will be discussing the connection between Fibonacci sequence (a series of numbers where every number is equal to the sum of two numbers before it) and Golden ratio. Secondly, how this mathematical idea shows up in a nature, such as sunflower and human DNA.

From Golden to Platinum

The extended“Golden Week”holiday,made more lustrous by the Hangzhou Asian Games and supporting policies,saw thriving tourism across China.

A Golden Relationship Gears Up for a New Takeoff

An Exclusive Interview with Greek Minister of Infrastructure and Transport Christos Staikouras.GREEK Prime Minister Kyriakos Mitsotakis paid an official visit to China on November 2-3,2023,ahead of the sixth China International Import Expo(CIIE).It was his second official visit to China.The first one was made during the second CIIE in November 2019,less than half a year after he took office.The two trips show the importance the Greek prime minister gives to Greece-China relations and the CIIE.

Second Approximation of the Generalized Planetary Equation Based upon Golden Metric Tensors

In this paper, we consider the Post Einstein Planetary equation of motion. We succeeded in offering a solution using second approximation method, in which we obtained eight exact mathematical solutions that rebel amazing theoretical results. To the order of C-2, two of these exact solutions are reduced to the approximate solutions from the method of successive approximations.

Double Delight China’s ‘Super Golden Week’ brings lively tapestry of tourism

Travelling in China,especially during the annual National Day holiday,also known as Golden Week,offers an unmissable spectacle-the travellers themselves.This time of year,bathed in the gentle embrace of early autumn,boasts delightful weather and offers the gift of an extended holiday,beckoning people outdoors.With China’s vast population,as people from all walks of life set forth on their trips,the fascinating panorama of bustling crowds becomes an integral part of the travel experience.