膝沟藻毒素Gonyautoxins的分离纯化

目的 从微小亚历山大藻 (AlexandriummimutumHalim)Amtk 2中分离纯化膝沟藻毒素gonyautoxins。方法用凝胶色谱、离子交换色谱等方法从人工培养的微小亚历山大藻Amtk 2酸酒精萃取物中分离纯化膝沟藻毒素。结果 从 10 0LAmtk 2培养液中获得 ( 6 74± 0 31)× 10 9个藻细胞 ,其酸酒精萃取物经凝胶色谱分离首次在国内得到膝沟藻毒素GTX 4 ,GTX 1,GTX 3和GTX 2混合物 ( 2 9 5 9± 0 2 8)mg。从此混合物中取出 4 0 6mg经两次离子交换色谱纯化得到纯GTX 4 ( 0 4 0± 0 0 0 2 )mg ,GTX 1( 5 95± 0 0 3)× 10 - 2 mg ,GTX 3( 6 92± 0 0 5 )× 10 - 4mg和GTX 2 ( 0 11±0 0 0 5 )mg。结论 人工培养的微小亚历山大藻Amtk 2酸酒精萃取物通过凝胶色谱和两次离子交换色谱分离能够得到纯的膝沟藻毒素GTX 4 ,GTX 1,GTX 3和GTX 2。

Isolation and Purification of Gonyautoxins from Two Strain of Alexandrium minutum Halim

Aim To study isolation and purification of Gonyautoxins from two differentstrains of Alexandrium minutum Halim. Methods Ethanol extracts of the two culture strains ofAlexandrium minutum Halim were fractionated by gel filtration and ion exchange chromatography toobtain gonyautoxins. The toxin profiles were tested by HPLC method. Results Both strains containgonyautoxin-1, gonyautoxin-2, gonyautoxin-3 and gonyautoxin-4. The total gonyautoxin content ofAmtk4 strain is almost 10 times higher than that of Amtk2 strain. Conclusion Alexandrium minutumHalim Amtk4 strain is suitable for the preparation of Gonyautoxins.