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根癌农杆菌介导大果西番莲基因转化
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作者 余贤美 刘志昕 +1 位作者 曾华金 彭存智 《热带作物学报》 CSCD 2003年第4期18-22,共5页
以大果西番莲种子无菌苗的子叶和下胚轴为外植体,在添加不同质量浓度BA和IAA的MS系列培养基上进行不定芽的诱导,确定MS+BA2.0mg·L^(-1)+IAA0.1 mg·L^(-1)为最适的不定芽诱导培养基,下胚轴、子叶外植体诱导芽分化率分别为88.33... 以大果西番莲种子无菌苗的子叶和下胚轴为外植体,在添加不同质量浓度BA和IAA的MS系列培养基上进行不定芽的诱导,确定MS+BA2.0mg·L^(-1)+IAA0.1 mg·L^(-1)为最适的不定芽诱导培养基,下胚轴、子叶外植体诱导芽分化率分别为88.33%,90.00%。将诱导出的不定芽接种在添加不同质量浓度IBA和NAA的MS系列培养基上,进行不定根的诱导,确定MS+NAA0.5mg·L^(-1)+IBA0.2mg·L^(-1)为最适的不定根诱导培养基,生根率达92.86%。用三亲交配法将质粒pBICr先转移到E.coli DH_5α,然后转移到根癌农杆菌。利用含有NPT-Ⅱ基因和CaMV35S启动子控制下的黄瓜花叶病毒外壳蛋白(CMV-CP)反义基因的表达载体,通过根癌农杆菌介导法将其导入西番莲受体细胞,在质量浓度为50mg·L^(-1)的Kan^+选择压力下培养,获得大果西番莲CP转基因再生植株。 展开更多
关键词 大果西番莲 基因转化 农杆菌 不定芽 无菌苗 培养基 压力强度
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Effects of chitosan coatings fused with medicinal plant extracts on postharvest quality and storage stability of purple passion fruit(Passiflora edulis var.Ester)
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作者 Kwanele A.Nxumalo Olaniyi A.Fawole 《Food Quality and Safety》 SCIE CSCD 2022年第1期93-111,共19页
Chitosan edible coating(Ch;2%,mass concentration)enriched with 2%of 0.1 mg/L Bidens pilosa(Ch+B),Lippia javanica(Ch+L),Syzygium cordatum(Ch+S),or Ximenia caffra(Ch+X)was applied as a composite edible coating in allevi... Chitosan edible coating(Ch;2%,mass concentration)enriched with 2%of 0.1 mg/L Bidens pilosa(Ch+B),Lippia javanica(Ch+L),Syzygium cordatum(Ch+S),or Ximenia caffra(Ch+X)was applied as a composite edible coating in alleviating shrivel and maintaining the quality of purple passion fruit(Passiflora edulis var.Ester).Treated fruit was dipped for 3 min in the coating solution,and control fruit was dipped in distilled water.The fruit were stored at(8±2)℃and 90%±5%relative humidity(RH)for 32 d.Sampling was done every 8 d plus 3 d(20±2)℃and(50%±5%)RH to simulate retail conditions.Efficacy of medicinal plant extracts in the chitosan matrix varied;lower ethylene production(82.42μL C_(2)H_(4)/(kg·h))was seen in fruit coated with Ch+S,and the lowest respiration rate(75 mL CO_(2)/(kg·h))was observed in fruit coated with Ch+B.The control fruit showed the highest ethylene production(84.90μL C_(2)H_(4)/(kg·h))and respiration rate(117.98 mL CO_(2)/(kg·h)).Fruit coated with Ch+B had the lowest weight loss(41.67%),higher juice content(60.13%)and BrimA(3.31);while the control fruit had the highest weight loss(88.03%),lowest juice content(21.90%),and BrimA(2.49).Shrivel incidence was lowest(23.70%)on fruit coated with Ch+L and highest(83.30%)on the control fruit.Fruit coated with Ch+X had the lowest electrolyte leakage(71.40%),while the control fruit had the highest(91.97%).Fruit coated with chitosan alone performed better than the control fruit but did not exceed the quality of composite chitosan-coated fruit.Based on the principal component analysis,it can be concluded that passion fruit coated with Ch+B was more effective in alleviating shrivel incidence,better maintained the quality ofpassion fruit duringstorage,andshows potential for commercial applications. 展开更多
关键词 Quality attributes anthocyanins perishability RESPIRATION granadilla
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