Rice leaffolder,Cnaphalocrocis medinalis(Guenée),has become a major pest throughout the rice cultivating areas of China and caused severe damage to rice production.Cnaphalocrocis medinalis granulovirus(CnmeGV),a ...Rice leaffolder,Cnaphalocrocis medinalis(Guenée),has become a major pest throughout the rice cultivating areas of China and caused severe damage to rice production.Cnaphalocrocis medinalis granulovirus(CnmeGV),a naturally occurring baculovirus,is revealed as a potential microbial agent for the pest control.Field applications of CnmeGV were conducted against rice leaffolder larvae in rice paddies.CnmeGV infected the larvae not only in the current generation but also in the successive generation,resulting in a sustained infection in the larva population for at least 48 days.Under diferent concentrations of CnmeGV(7.5×1011 and 1.125×1012 occlusion body(OB)ha-1)at 30 days after spraying,larval population reduced up to 76.32%and rice leaf rolled rate kept in 15.42%.Simultaneously,CnmeGV had no impact on arthropod predators of C.medinalis,with abundances ranging from 2.39 to 3.79 per ten hills.These results revealed that CnmeGV is suitable as a bio-pesticide for rice leaffolder management in rice paddies.展开更多
To better understand the origination and evolution of lef-3 gene from baculovirus genome and determine the relationships between various viruses at molecular level, late expression factor 3 gene （lef-3） fragments of...To better understand the origination and evolution of lef-3 gene from baculovirus genome and determine the relationships between various viruses at molecular level, late expression factor 3 gene （lef-3） fragments of Pieris rapae granulovirus were obtained through conventional PCR method and sequenced after cloned into T-vector. Then, bioinformatics analysis on lef-3 gene and its encoding sequences were conducted by using bid-softs. Four mutations were appeared in the ORF of cloned lef-3 gene, which did not alter the characteristics of amino acids. It was inferred that PiraGV LEF-3 protein contained 399 amino acids, the molecular weight of which was 3.99 kD. Prediction of the LEF-3 advanced structure and homology comparison between other LEF-3 from various baculoviruses showed that the lef-3 gene might encode the single-stranded DNA-binding protein. The result of BLAST revealed that the lef-3 gene only existed in Lepidoptera host for the baculovirus genome, and the evolution analysis illustrated that lef-3 gene could be divided into 3 groups including one granulovirus （GV） group and 2 nucleopolyhedrovirus （NPV） groups. The selection pressure analysis of GV lef-3 gene coding region showed that the majority of lef-3 genes performed negative selection, while the Ka/Ks differed from different lef-3 gene, to some extent, which also performed positive selection. The origination analysis revealed that lef-3 gene of baculovirus might derive from bacteria. The lef-3 gene of PiraGV was cloned successfully and the possible patterns of origination and evolution were speculated through bioinformatics analysis.展开更多
通过PCR方法获得家蚕核型多角体病毒(Bombyx mori nuclearpolyhedrosis virus,BmNPV)的蜕皮甾体尿苷二磷酸葡萄糖基转移酶基因(egt)片段,序列分析表明该片段带有EGT的完整ORF,推测的多肽可形成EGT结构域的高级结构。为了研究egt的起源,...通过PCR方法获得家蚕核型多角体病毒(Bombyx mori nuclearpolyhedrosis virus,BmNPV)的蜕皮甾体尿苷二磷酸葡萄糖基转移酶基因(egt)片段,序列分析表明该片段带有EGT的完整ORF,推测的多肽可形成EGT结构域的高级结构。为了研究egt的起源,利用家蚕基因组数据库,电子克隆了多个家蚕尿苷二磷酸葡萄糖醛酸转移酶(UGT)基因,在此基础上进行了进化分析,表明BmNPV的EGT为antennal-enriched型UGT;推测核型多角体病毒(nucleopolyhedrivirus,NPV)和颗粒体病毒(granulovirus,GV)的egt基因在进化上来源于昆虫的UGT基因,但GV的egt基因在进化上的起源可能要早于NPV的egt基因;可能在昆虫祖先种进化形成不同昆虫目的某一时期,杆状病毒的祖先种从昆虫中获得了antennal-enriched型UGT基因,并进化为egt基因。家蚕的部分UGT基因与转座子元件连锁的基因组结构特点反映了杆状病毒的egt基因可能通过转座子的传递而获得。展开更多
基金supported by the National Natural Science Foundation of China (31071740 and 31701792)the Jiangsu Science Foundation of China (BK20181283)the Jiangsu Agricultural Science and Technology Innovation Fund, China (ZX(17)2002)
文摘Rice leaffolder,Cnaphalocrocis medinalis(Guenée),has become a major pest throughout the rice cultivating areas of China and caused severe damage to rice production.Cnaphalocrocis medinalis granulovirus(CnmeGV),a naturally occurring baculovirus,is revealed as a potential microbial agent for the pest control.Field applications of CnmeGV were conducted against rice leaffolder larvae in rice paddies.CnmeGV infected the larvae not only in the current generation but also in the successive generation,resulting in a sustained infection in the larva population for at least 48 days.Under diferent concentrations of CnmeGV(7.5×1011 and 1.125×1012 occlusion body(OB)ha-1)at 30 days after spraying,larval population reduced up to 76.32%and rice leaf rolled rate kept in 15.42%.Simultaneously,CnmeGV had no impact on arthropod predators of C.medinalis,with abundances ranging from 2.39 to 3.79 per ten hills.These results revealed that CnmeGV is suitable as a bio-pesticide for rice leaffolder management in rice paddies.
基金supported by the National High Technology Research and Development Program of China(2006AA10A118)
文摘To better understand the origination and evolution of lef-3 gene from baculovirus genome and determine the relationships between various viruses at molecular level, late expression factor 3 gene （lef-3） fragments of Pieris rapae granulovirus were obtained through conventional PCR method and sequenced after cloned into T-vector. Then, bioinformatics analysis on lef-3 gene and its encoding sequences were conducted by using bid-softs. Four mutations were appeared in the ORF of cloned lef-3 gene, which did not alter the characteristics of amino acids. It was inferred that PiraGV LEF-3 protein contained 399 amino acids, the molecular weight of which was 3.99 kD. Prediction of the LEF-3 advanced structure and homology comparison between other LEF-3 from various baculoviruses showed that the lef-3 gene might encode the single-stranded DNA-binding protein. The result of BLAST revealed that the lef-3 gene only existed in Lepidoptera host for the baculovirus genome, and the evolution analysis illustrated that lef-3 gene could be divided into 3 groups including one granulovirus （GV） group and 2 nucleopolyhedrovirus （NPV） groups. The selection pressure analysis of GV lef-3 gene coding region showed that the majority of lef-3 genes performed negative selection, while the Ka/Ks differed from different lef-3 gene, to some extent, which also performed positive selection. The origination analysis revealed that lef-3 gene of baculovirus might derive from bacteria. The lef-3 gene of PiraGV was cloned successfully and the possible patterns of origination and evolution were speculated through bioinformatics analysis.
文摘通过PCR方法获得家蚕核型多角体病毒(Bombyx mori nuclearpolyhedrosis virus,BmNPV)的蜕皮甾体尿苷二磷酸葡萄糖基转移酶基因(egt)片段,序列分析表明该片段带有EGT的完整ORF,推测的多肽可形成EGT结构域的高级结构。为了研究egt的起源,利用家蚕基因组数据库,电子克隆了多个家蚕尿苷二磷酸葡萄糖醛酸转移酶(UGT)基因,在此基础上进行了进化分析,表明BmNPV的EGT为antennal-enriched型UGT;推测核型多角体病毒(nucleopolyhedrivirus,NPV)和颗粒体病毒(granulovirus,GV)的egt基因在进化上来源于昆虫的UGT基因,但GV的egt基因在进化上的起源可能要早于NPV的egt基因;可能在昆虫祖先种进化形成不同昆虫目的某一时期,杆状病毒的祖先种从昆虫中获得了antennal-enriched型UGT基因,并进化为egt基因。家蚕的部分UGT基因与转座子元件连锁的基因组结构特点反映了杆状病毒的egt基因可能通过转座子的传递而获得。
