The method of immunohistochemistry assay for the detection of scrapie in Chinese Short-tailed Han sheep was established using monoclonal antibody. Genomic DNA was isolated from Chinese Short-tailed Hart sheep blood. U...The method of immunohistochemistry assay for the detection of scrapie in Chinese Short-tailed Han sheep was established using monoclonal antibody. Genomic DNA was isolated from Chinese Short-tailed Hart sheep blood. Using the polymerase chain reaction technique, PrP27-30 gene sequence was amplified from Chinese Short-tailed Han sheep genomic DNA. By recombinant DNA technology, the recombinant protein of Chinese Short-tailed Han sheep PrP27-30 was obtained. Then, using standard methodology of myeloma cell fusion, a panel of monoclonal antibodies was generated. With mAbs, scrapie in Chinese Short-tailed Han sheep was detected by immunohistochemistry assay. The recombinant protein of Chinese Short-tailed Han sheep PrP27-30 was obtained and a panel of six hybridoma cell lines secreting specific antibodies to Chinese Short-tailed Han sheep PrP27-30 related to scrapie was obtained with one fusion between myeloma Sp2/0 and spleen ceils from mice immunized with the purified recombinant protein. Four hybridoma cell lines can be used in immunohistochemistry assay for the detection of scrapie in Chinese Short-tailed Han sheep. So that the special monoclonal antibody developed in author＇s institute can be used to detect PrP^sc of scrapie in Chinese Short-tailed Han sheep by immunohistochemistry in China.展开更多
Winter food constitution and favorite level of domestic sheep were studied with the microhistological analysis technique of feces in Hulunbeir Grassland. some problems were inquired into, such as the quality state of ...Winter food constitution and favorite level of domestic sheep were studied with the microhistological analysis technique of feces in Hulunbeir Grassland. some problems were inquired into, such as the quality state of local herbage resource, improvement of pasture, rational use of herbage, etc. The results showed that the staple food of domestic sheep in winter was Gramineae, which made up 73.3 percent of the total foraging species. Fabaceae was second, 11.6 per cent. Composite took third place, 7.4 per cent. The proportion of other species were very small.展开更多
The long-term productivity of a soil is greatly influenced by cation exchange capacity（CEC）.Moreover,interactions between dominant base cations and other nutrients are important for the health and stability of grass...The long-term productivity of a soil is greatly influenced by cation exchange capacity（CEC）.Moreover,interactions between dominant base cations and other nutrients are important for the health and stability of grassland ecosystems.Soil exchangeable base cations and cation ratios were examined in a 11-year experiment with sheep manure application rates 0–1,500 g/（m2？a） in a semi-arid steppe in Inner Mongolia of China,aiming to clarify the relationships of base cations with soil p H,buffer capacity and fertility.Results showed that CEC and contents of exchangeable calcium（Ca2＋）,magnesium（Mg2＋）,potassium（K＋） and sodium（Na＋） were significantly increased,and Ca2＋ saturation tended to decrease,while K＋ saturation tended to increase with the increases of sheep manure application rates.The Ca2＋/Mg2＋ and Ca2＋/K＋ ratios decreased,while Mg2＋,K＋ and Na＋ saturations increased with increasing manure application rates.Both base cations and CEC were significantly and positively correlated with soil organic carbon（SOC） and soil p H.The increases of SOC and soil p H would be the dominant factors that contribute to the increase of cations in soil.On a comparison with the initial soil p H before the experiment,we deduced that sheep manure application could partly buffer soil p H decrease potentially induced by atmospheric deposition of nitrogen and sulfur.Our results indicate that sheep manure application is beneficial to the maintenance of base cations and the buffering of soil acidification,and therefore can improve soil fertility in the semi-arid steppes of northeastern China.展开更多
The growth of grass industry is essential for meeting human needs for proteins and sustaining grassland ecosystems(Bengtsson et al., 2019). Leymus chinensis, also known as sheepgrass, is a perennial Triticeae forage r...The growth of grass industry is essential for meeting human needs for proteins and sustaining grassland ecosystems(Bengtsson et al., 2019). Leymus chinensis, also known as sheepgrass, is a perennial Triticeae forage renowned for its adaptability and top-notch forage quality(Li et al., 2013).展开更多
基金the National Natural Sci-ence Foundation of China (C02030606)948 Project from Agriculture Ministry of China (2001-366)
文摘The method of immunohistochemistry assay for the detection of scrapie in Chinese Short-tailed Han sheep was established using monoclonal antibody. Genomic DNA was isolated from Chinese Short-tailed Hart sheep blood. Using the polymerase chain reaction technique, PrP27-30 gene sequence was amplified from Chinese Short-tailed Han sheep genomic DNA. By recombinant DNA technology, the recombinant protein of Chinese Short-tailed Han sheep PrP27-30 was obtained. Then, using standard methodology of myeloma cell fusion, a panel of monoclonal antibodies was generated. With mAbs, scrapie in Chinese Short-tailed Han sheep was detected by immunohistochemistry assay. The recombinant protein of Chinese Short-tailed Han sheep PrP27-30 was obtained and a panel of six hybridoma cell lines secreting specific antibodies to Chinese Short-tailed Han sheep PrP27-30 related to scrapie was obtained with one fusion between myeloma Sp2/0 and spleen ceils from mice immunized with the purified recombinant protein. Four hybridoma cell lines can be used in immunohistochemistry assay for the detection of scrapie in Chinese Short-tailed Han sheep. So that the special monoclonal antibody developed in author＇s institute can be used to detect PrP^sc of scrapie in Chinese Short-tailed Han sheep by immunohistochemistry in China.
文摘Winter food constitution and favorite level of domestic sheep were studied with the microhistological analysis technique of feces in Hulunbeir Grassland. some problems were inquired into, such as the quality state of local herbage resource, improvement of pasture, rational use of herbage, etc. The results showed that the staple food of domestic sheep in winter was Gramineae, which made up 73.3 percent of the total foraging species. Fabaceae was second, 11.6 per cent. Composite took third place, 7.4 per cent. The proportion of other species were very small.
基金funded by the National Natural Science Foundation of China (41371251,31370009)the National Basic Research Program of China (2011CB403204)
文摘The long-term productivity of a soil is greatly influenced by cation exchange capacity（CEC）.Moreover,interactions between dominant base cations and other nutrients are important for the health and stability of grassland ecosystems.Soil exchangeable base cations and cation ratios were examined in a 11-year experiment with sheep manure application rates 0–1,500 g/（m2？a） in a semi-arid steppe in Inner Mongolia of China,aiming to clarify the relationships of base cations with soil p H,buffer capacity and fertility.Results showed that CEC and contents of exchangeable calcium（Ca2＋）,magnesium（Mg2＋）,potassium（K＋） and sodium（Na＋） were significantly increased,and Ca2＋ saturation tended to decrease,while K＋ saturation tended to increase with the increases of sheep manure application rates.The Ca2＋/Mg2＋ and Ca2＋/K＋ ratios decreased,while Mg2＋,K＋ and Na＋ saturations increased with increasing manure application rates.Both base cations and CEC were significantly and positively correlated with soil organic carbon（SOC） and soil p H.The increases of SOC and soil p H would be the dominant factors that contribute to the increase of cations in soil.On a comparison with the initial soil p H before the experiment,we deduced that sheep manure application could partly buffer soil p H decrease potentially induced by atmospheric deposition of nitrogen and sulfur.Our results indicate that sheep manure application is beneficial to the maintenance of base cations and the buffering of soil acidification,and therefore can improve soil fertility in the semi-arid steppes of northeastern China.
文摘The growth of grass industry is essential for meeting human needs for proteins and sustaining grassland ecosystems(Bengtsson et al., 2019). Leymus chinensis, also known as sheepgrass, is a perennial Triticeae forage renowned for its adaptability and top-notch forage quality(Li et al., 2013).
